Rroxscaffold_7G00159040

Phytosulfokines

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003745
Physical Location & Seq
Forward (+)
2344557 .. 2345177
621 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_7G00159040.1

Sequence Viewer

Length: 276 bp
ATGGCCAAGTTAACCGCCCTTTTCACCATCGCCGCTCTCCTCCTCCTCCTCCTCCTCCTCAGCTCTGAGCTAACCTCCGCGGTTCGCCCAGAGCCTGCTTTCACCAACAGCGCGCTCAAAACCGCCCAACACCAAGATGTAGGAGCAGATCAGAACATATTAGCCCAGGATGGTGAGGTTAGCTGTGAAGGAGTGAACGAGGAGGAGTGCCTGATGCGAAGAACCCTGGCTGCTCATGTGGATTATATATATACTCAGAAGCACAACAAGCCTTGA

Protein Analysis

91

Amino Acids

9.95

Weight (kDa)

5.17

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSK PF06404 16 - 90 1.9e-17 Phytosulfokine precursor protein (PSK)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 35
AccII CGCG 2 cut(s) 80, 113
AciI CCGC 5 cut(s) 15, 33, 78, 80, 123
AcoI YGGCCR 1 cut(s) 3
AjnI CCWGG 2 cut(s) 165, 225
AluBI AGCT 3 cut(s) 63, 70, 183
AluI AGCT 3 cut(s) 63, 70, 183
AlwNI CAGNNNCTG 1 cut(s) 95
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 230
AspLEI GCGC 2 cut(s) 113, 115
AsuHPI GGTGA 3 cut(s) 16, 94, 185
BalI TGGCCA 1 cut(s) 5
BbvCI CCTCAGC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 217
BccI CCATC 2 cut(s) 35, 164
BciT130I CCWGG 2 cut(s) 167, 227
BisI GCNGC 2 cut(s) 33, 231
BlsI GCNGC 2 cut(s) 34, 232
Bme1390I CCNGG 2 cut(s) 167, 227
BmrFI CCNGG 2 cut(s) 167, 227
BmsI GCATC 1 cut(s) 204
BplI GAGNNNNNCTC 2 cut(s) 59, 91
Bpu10I CCTNAGC 1 cut(s) 59
BsaJI CCNNGG 3 cut(s) 78, 165, 225
BseBI CCWGG 2 cut(s) 167, 227
BseDI CCNNGG 3 cut(s) 78, 165, 225
BseGI GGATG 1 cut(s) 175
BseMII CTCAG 3 cut(s) 57, 73, 269
BsePI GCGCGC 1 cut(s) 111
BseRI GAGGAG 9 cut(s) 29, 32, 35, 38, 41, 44, 47, 215, 218
BseXI GCAGC 1 cut(s) 217
Bsh1236I CGCG 2 cut(s) 80, 113
BshFI GGCC 1 cut(s) 5
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 148
BspACI CCGC 5 cut(s) 15, 33, 78, 80, 123
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 58, 72, 268
BspFNI CGCG 2 cut(s) 80, 113
BsrBI CCGCTC 1 cut(s) 35
BssECI CCNNGG 3 cut(s) 78, 165, 225
BssHII GCGCGC 1 cut(s) 111
BssMI GATC 1 cut(s) 148
Bst2UI CCWGG 2 cut(s) 167, 227
BstC8I GCNNGC 2 cut(s) 96, 113
BstDEI CTNAG 3 cut(s) 59, 66, 255
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 1 cut(s) 175
BstFNI CGCG 2 cut(s) 80, 113
BstHHI GCGC 2 cut(s) 113, 115
BstKTI GATC 1 cut(s) 151
BstMBI GATC 1 cut(s) 148
BstMWI GCNNNNNNNGC 1 cut(s) 268
BstNI CCWGG 2 cut(s) 167, 227
BstSCI CCNGG 2 cut(s) 165, 225
BstUI CGCG 2 cut(s) 80, 113
BstV1I GCAGC 1 cut(s) 217
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 78
BtgZI GCGATG 1 cut(s) 13
BtsCI GGATG 1 cut(s) 175
Cac8I GCNNGC 2 cut(s) 96, 113
CaiI CAGNNNCTG 1 cut(s) 95
CfoI GCGC 2 cut(s) 113, 115
Cfr42I CCGCGG 1 cut(s) 81
CviAII CATG 1 cut(s) 236
CviJI RGCY 8 cut(s) 5, 63, 70, 94, 164, 183, 230, 271
CviKI_1 RGCY 8 cut(s) 5, 63, 70, 94, 164, 183, 230, 271
DdeI CTNAG 3 cut(s) 59, 66, 255
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 3
EcoRII CCWGG 2 cut(s) 165, 225
FaeI CATG 1 cut(s) 239
FaiI YATR 6 cut(s) 158, 237, 246, 248, 250, 252
FatI CATG 1 cut(s) 235
Fnu4HI GCNGC 2 cut(s) 33, 231
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 2 cut(s) 33, 231
GlaI GCGC 2 cut(s) 112, 114
GluI GCNGC 2 cut(s) 33, 231
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 2 cut(s) 113, 115
Hin1II CATG 1 cut(s) 239
Hin6I GCGC 2 cut(s) 111, 113
HinP1I GCGC 2 cut(s) 111, 113
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HpaI GTTAAC 1 cut(s) 12
HphI GGTGA 3 cut(s) 16, 94, 185
Hpy166II GTNNAC 2 cut(s) 12, 196
Hpy188I TCNGA 3 cut(s) 67, 153, 258
Hpy8I GTNNAC 2 cut(s) 12, 196
HpyAV CCTTC 1 cut(s) 182
HpyF10VI GCNNNNNNNGC 1 cut(s) 268
HpyF3I CTNAG 3 cut(s) 59, 66, 255
Hsp92II CATG 1 cut(s) 239
HspAI GCGC 2 cut(s) 111, 113
KspAI GTTAAC 1 cut(s) 12
KspI CCGCGG 1 cut(s) 81
Kzo9I GATC 1 cut(s) 148
LmnI GCTCC 1 cut(s) 143
LpnPI CCDG 7 cut(s) 102, 108, 152, 179, 212, 224, 239
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 1 cut(s) 204
MalI GATC 1 cut(s) 150
MbiI CCGCTC 1 cut(s) 35
MboI GATC 1 cut(s) 148
MboII GAAGA 1 cut(s) 231
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 11
MslI CAYNNNNRTG 1 cut(s) 135
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 80
MspR9I CCNGG 2 cut(s) 167, 227
MvaI CCWGG 2 cut(s) 167, 227
MvnI CGCG 2 cut(s) 80, 113
MwoI GCNNNNNNNGC 1 cut(s) 268
NdeII GATC 1 cut(s) 148
NlaIII CATG 1 cut(s) 239
PauI GCGCGC 1 cut(s) 111
PkrI GCNGC 2 cut(s) 34, 232
Psp6I CCWGG 2 cut(s) 165, 225
PspGI CCWGG 2 cut(s) 165, 225
PstNI CAGNNNCTG 1 cut(s) 95
PteI GCGCGC 1 cut(s) 111
RseI CAYNNNNRTG 1 cut(s) 135
SacII CCGCGG 1 cut(s) 81
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 2 cut(s) 33, 231
Sau3AI GATC 1 cut(s) 148
ScrFI CCNGG 2 cut(s) 167, 227
SetI ASST 5 cut(s) 65, 72, 77, 180, 185
SfaNI GCATC 1 cut(s) 204
Sfr303I CCGCGG 1 cut(s) 81
SgrBI CCGCGG 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 135
SsiI CCGC 5 cut(s) 15, 33, 78, 80, 123
StyD4I CCNGG 2 cut(s) 165, 225
TauI GCSGC 1 cut(s) 35
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TseI GCWGC 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.