Rh6DG490300

Phytosulfokines

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003745
Physical Location & Seq
Reverse (-)
65038967 .. 65039671
705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG490300.1

Sequence Viewer

Length: 267 bp
ATGGCCAAGTTAACCGCCCTTTTCACCATCGCCGCTCTCCTCCTCCTCCTCAGCTCTGATCTAACCTCCGCGGTTCGCCCGGAGCCTGCTTTCTCCAACAGCGCGCTCAAAACCGCCCAACACCAAGATGTAGGAGCAGATCAGAACATTAATGCCGAGGATGGCGAGGTTAGCTGTGAAGGAGTGAACGAGGAGGAGTGCCTGATGCGAAGAACCCTGGCTGCTCATGTGGATTATATATATACTCAGAAGCACAACAAGCCTTGA

Protein Analysis

88

Amino Acids

9.59

Weight (kDa)

4.95

Isoelectric Point (pI)

49.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSK PF06404 13 - 87 1.7e-17 Phytosulfokine precursor protein (PSK)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 35
AccII CGCG 2 cut(s) 71, 104
AciI CCGC 5 cut(s) 15, 33, 69, 71, 114
AcoI YGGCCR 1 cut(s) 3
AjnI CCWGG 1 cut(s) 216
AluBI AGCT 2 cut(s) 54, 174
AluI AGCT 2 cut(s) 54, 174
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 221
AseI ATTAAT 1 cut(s) 150
AspLEI GCGC 2 cut(s) 104, 106
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 16
BalI TGGCCA 1 cut(s) 5
BbvCI CCTCAGC 1 cut(s) 50
BbvI GCAGC 1 cut(s) 208
BccI CCATC 2 cut(s) 35, 155
BciT130I CCWGG 1 cut(s) 218
BcnI CCSGG 1 cut(s) 80
BisI GCNGC 2 cut(s) 33, 222
BlsI GCNGC 2 cut(s) 34, 223
Bme1390I CCNGG 2 cut(s) 80, 218
BmiI GGNNCC 1 cut(s) 84
BmrFI CCNGG 2 cut(s) 80, 218
BmsI GCATC 1 cut(s) 195
Bpu10I CCTNAGC 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 80
BsaJI CCNNGG 3 cut(s) 69, 156, 216
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 3 cut(s) 69, 156, 216
BseGI GGATG 1 cut(s) 166
BseMII CTCAG 2 cut(s) 64, 260
BsePI GCGCGC 1 cut(s) 102
BseRI GAGGAG 6 cut(s) 29, 32, 35, 38, 206, 209
BseXI GCAGC 1 cut(s) 208
Bsh1236I CGCG 2 cut(s) 71, 104
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 80
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 2 cut(s) 58, 139
BspACI CCGC 5 cut(s) 15, 33, 69, 71, 114
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 63, 259
BspFNI CGCG 2 cut(s) 71, 104
BspLI GGNNCC 1 cut(s) 84
BsrBI CCGCTC 1 cut(s) 35
BssECI CCNNGG 3 cut(s) 69, 156, 216
BssHII GCGCGC 1 cut(s) 102
BssMI GATC 2 cut(s) 58, 139
Bst2UI CCWGG 1 cut(s) 218
BstC8I GCNNGC 2 cut(s) 87, 104
BstDEI CTNAG 2 cut(s) 50, 246
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 1 cut(s) 166
BstFNI CGCG 2 cut(s) 71, 104
BstHHI GCGC 2 cut(s) 104, 106
BstKTI GATC 2 cut(s) 61, 142
BstMBI GATC 2 cut(s) 58, 139
BstMWI GCNNNNNNNGC 2 cut(s) 171, 259
BstNI CCWGG 1 cut(s) 218
BstSCI CCNGG 2 cut(s) 78, 216
BstUI CGCG 2 cut(s) 71, 104
BstV1I GCAGC 1 cut(s) 208
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 69
BtgZI GCGATG 1 cut(s) 13
BtsCI GGATG 1 cut(s) 166
Cac8I GCNNGC 2 cut(s) 87, 104
CfoI GCGC 2 cut(s) 104, 106
Cfr42I CCGCGG 1 cut(s) 72
CviAII CATG 1 cut(s) 227
CviJI RGCY 6 cut(s) 5, 54, 85, 174, 221, 262
CviKI_1 RGCY 6 cut(s) 5, 54, 85, 174, 221, 262
DdeI CTNAG 2 cut(s) 50, 246
DpnI GATC 2 cut(s) 60, 141
DpnII GATC 2 cut(s) 58, 139
EaeI YGGCCR 1 cut(s) 3
EcoRII CCWGG 1 cut(s) 216
FaeI CATG 1 cut(s) 230
FaiI YATR 5 cut(s) 228, 237, 239, 241, 243
FatI CATG 1 cut(s) 226
Fnu4HI GCNGC 2 cut(s) 33, 222
FokI GGATG 1 cut(s) 173
Fsp4HI GCNGC 2 cut(s) 33, 222
GlaI GCGC 2 cut(s) 103, 105
GluI GCNGC 2 cut(s) 33, 222
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 80
HhaI GCGC 2 cut(s) 104, 106
Hin1II CATG 1 cut(s) 230
Hin6I GCGC 2 cut(s) 102, 104
HinP1I GCGC 2 cut(s) 102, 104
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HpaI GTTAAC 1 cut(s) 12
HpaII CCGG 1 cut(s) 80
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 12, 187
Hpy188I TCNGA 3 cut(s) 58, 144, 249
Hpy8I GTNNAC 2 cut(s) 12, 187
HpyAV CCTTC 1 cut(s) 173
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 259
HpyF3I CTNAG 2 cut(s) 50, 246
Hsp92II CATG 1 cut(s) 230
HspAI GCGC 2 cut(s) 102, 104
KspAI GTTAAC 1 cut(s) 12
KspI CCGCGG 1 cut(s) 72
Kzo9I GATC 2 cut(s) 58, 139
LmnI GCTCC 2 cut(s) 82, 134
LpnPI CCDG 5 cut(s) 93, 99, 203, 215, 230
Lsp1109I GCAGC 1 cut(s) 208
LweI GCATC 1 cut(s) 195
MalI GATC 2 cut(s) 60, 141
MbiI CCGCTC 1 cut(s) 35
MboI GATC 2 cut(s) 58, 139
MboII GAAGA 1 cut(s) 222
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 9 cut(s) 50, 53, 56, 59, 76, 151, 160, 184, 187
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 11, 150
MslI CAYNNNNRTG 1 cut(s) 126
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 2 cut(s) 80, 218
MvaI CCWGG 1 cut(s) 218
MvnI CGCG 2 cut(s) 71, 104
MwoI GCNNNNNNNGC 2 cut(s) 171, 259
NciI CCSGG 1 cut(s) 80
NdeII GATC 2 cut(s) 58, 139
NlaIII CATG 1 cut(s) 230
NlaIV GGNNCC 1 cut(s) 84
NmeAIII GCCGAG 1 cut(s) 181
PauI GCGCGC 1 cut(s) 102
PkrI GCNGC 2 cut(s) 34, 223
PshBI ATTAAT 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 216
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 84
PteI GCGCGC 1 cut(s) 102
RseI CAYNNNNRTG 1 cut(s) 126
SacII CCGCGG 1 cut(s) 72
SaqAI TTAA 2 cut(s) 11, 150
SatI GCNGC 2 cut(s) 33, 222
Sau3AI GATC 2 cut(s) 58, 139
ScrFI CCNGG 2 cut(s) 80, 218
SetI ASST 4 cut(s) 56, 68, 171, 176
SfaNI GCATC 1 cut(s) 195
Sfr303I CCGCGG 1 cut(s) 72
SgrBI CCGCGG 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 126
SsiI CCGC 5 cut(s) 15, 33, 69, 71, 114
StyD4I CCNGG 2 cut(s) 78, 216
TauI GCSGC 1 cut(s) 35
Tru1I TTAA 2 cut(s) 11, 150
Tru9I TTAA 2 cut(s) 11, 150
TseI GCWGC 1 cut(s) 221
VspI ATTAAT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.