pycom08g06550

Phytosulfokines

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003745
Physical Location & Seq
Forward (+)
5104750 .. 5105265
516 bp
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UTR
Exon/CDS
Intron
pycom08g06550.1

Sequence Viewer

Length: 258 bp
ATGGCGAAGCATATTATCACCTTCTTCACCGTAGCTCTCCTCCTCAACCTTCAACTCACAACCTTCGCTGCTCGTCCAGTCCCTGCTGCTTTCACCATCAACATTCTCAAAACCCAACACCAACAGGACATAGAAGCAAAGATCGCTGCGGCGGAGGAAGGCGGGAGCTGCGAAGGAGTAGATAAGGAGGAGTGCCTGATGAGGAGGACGCTGGCGGCTCATTTGGATTATATATACACCCAGAAGCACAAGCCTTGA

Protein Analysis

86

Amino Acids

9.5

Weight (kDa)

6.89

Isoelectric Point (pI)

69.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSK PF06404 35 - 85 2.2e-16 Phytosulfokine precursor protein (PSK)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 149, 152, 162, 215
AgsI TTSAA 1 cut(s) 53
AluBI AGCT 2 cut(s) 35, 168
AluI AGCT 2 cut(s) 35, 168
AlwNI CAGNNNCTG 1 cut(s) 83
ApeKI GCWGC 4 cut(s) 68, 86, 146, 168
AsuHPI GGTGA 3 cut(s) 10, 19, 85
BbvI GCAGC 4 cut(s) 55, 73, 133, 155
BccI CCATC 1 cut(s) 104
BisI GCNGC 6 cut(s) 69, 87, 147, 150, 169, 216
BlsI GCNGC 6 cut(s) 70, 88, 148, 151, 170, 217
Bse1I ACTGG 1 cut(s) 77
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 4 cut(s) 29, 32, 203, 217
BseXI GCAGC 4 cut(s) 55, 73, 133, 155
BslFI GGGAC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 65
Bsp143I GATC 1 cut(s) 141
BspACI CCGC 4 cut(s) 149, 152, 162, 215
BsrI ACTGG 1 cut(s) 77
BssMI GATC 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 31
BstC8I GCNNGC 1 cut(s) 213
BstKTI GATC 1 cut(s) 144
BstMBI GATC 1 cut(s) 141
BstMWI GCNNNNNNNGC 2 cut(s) 143, 168
BstV1I GCAGC 4 cut(s) 55, 73, 133, 155
Cac8I GCNNGC 1 cut(s) 213
CaiI CAGNNNCTG 1 cut(s) 83
CseI GACGC 1 cut(s) 217
CviJI RGCY 4 cut(s) 35, 168, 218, 253
CviKI_1 RGCY 4 cut(s) 35, 168, 218, 253
DpnI GATC 1 cut(s) 143
DpnII GATC 1 cut(s) 141
EciI GGCGGA 1 cut(s) 167
FaiI YATR 5 cut(s) 12, 131, 231, 233, 235
FaqI GGGAC 1 cut(s) 65
FauI CCCGC 1 cut(s) 155
Fnu4HI GCNGC 6 cut(s) 69, 87, 147, 150, 169, 216
Fsp4HI GCNGC 6 cut(s) 69, 87, 147, 150, 169, 216
GluI GCNGC 6 cut(s) 69, 87, 147, 150, 169, 216
HgaI GACGC 1 cut(s) 217
HphI GGTGA 3 cut(s) 10, 19, 85
HpyAV CCTTC 5 cut(s) 31, 59, 73, 152, 167
HpyCH4III ACNGT 1 cut(s) 31
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 168
Kzo9I GATC 1 cut(s) 141
LmnI GCTCC 1 cut(s) 165
LpnPI CCDG 6 cut(s) 90, 96, 110, 197, 209, 254
Lsp1109I GCAGC 4 cut(s) 55, 73, 133, 155
MalI GATC 1 cut(s) 143
MboI GATC 1 cut(s) 141
MboII GAAGA 1 cut(s) 16
MnlI CCTC 6 cut(s) 50, 53, 148, 181, 195, 198
MwoI GCNNNNNNNGC 2 cut(s) 143, 168
NdeII GATC 1 cut(s) 141
PkrI GCNGC 6 cut(s) 70, 88, 148, 151, 170, 217
PstNI CAGNNNCTG 1 cut(s) 83
SatI GCNGC 6 cut(s) 69, 87, 147, 150, 169, 216
Sau3AI GATC 1 cut(s) 141
SetI ASST 5 cut(s) 23, 37, 51, 65, 170
SgeI CNNG 8 cut(s) 84, 89, 95, 137, 175, 208, 224, 253
SsiI CCGC 4 cut(s) 149, 152, 162, 215
TaaI ACNGT 1 cut(s) 31
TauI GCSGC 2 cut(s) 152, 218
TseI GCWGC 4 cut(s) 68, 86, 146, 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.