MD08G1079100.v1.1

Phytosulfokines

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003745
Physical Location & Seq
Forward (+)
6590799 .. 6591412
614 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1079100.v1.1.491

Sequence Viewer

Length: 258 bp
ATGGCGAAGCATATTATCACCTTCTTCACCGTAGCTCTCCTCCTCAACCTTCAACTCACAACCTTCGCTGCTCGTCCAGTCCCTGCTGCTTTTACCAACAACACTCTCAAAATCCAACACCAACAGGACATAGAAGCAAAGATCGCTGCAGCGGAGGAAGGCGGGAGCTGCCAAGGAGTAGAGAAGGAGGAGTGCCTGATGAGGAGGACGCTGGCGGCTCATTTGGATTATATATACACCCAGAAGCACAAGCCTTGA

Protein Analysis

86

Amino Acids

9.51

Weight (kDa)

7.84

Isoelectric Point (pI)

60.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSK PF06404 19 - 85 2.7e-16 Phytosulfokine precursor protein (PSK)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 152, 162, 215
AgsI TTSAA 1 cut(s) 53
AluBI AGCT 2 cut(s) 35, 168
AluI AGCT 2 cut(s) 35, 168
AlwNI CAGNNNCTG 1 cut(s) 83
ApeKI GCWGC 5 cut(s) 68, 86, 146, 149, 168
AsuHPI GGTGA 2 cut(s) 10, 19
BbvI GCAGC 5 cut(s) 55, 73, 133, 155, 161
BfmI CTRYAG 1 cut(s) 147
BisI GCNGC 6 cut(s) 69, 87, 147, 150, 169, 216
BlsI GCNGC 6 cut(s) 70, 88, 148, 151, 170, 217
BsaJI CCNNGG 1 cut(s) 172
Bse1I ACTGG 1 cut(s) 77
BseDI CCNNGG 1 cut(s) 172
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 4 cut(s) 29, 32, 203, 217
BseXI GCAGC 5 cut(s) 55, 73, 133, 155, 161
BslFI GGGAC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 65
Bsp143I GATC 1 cut(s) 141
BspACI CCGC 3 cut(s) 152, 162, 215
BspMAI CTGCAG 1 cut(s) 151
BsrI ACTGG 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 172
BssMI GATC 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 172
Bst4CI ACNGT 1 cut(s) 31
BstC8I GCNNGC 1 cut(s) 213
BstKTI GATC 1 cut(s) 144
BstMBI GATC 1 cut(s) 141
BstMWI GCNNNNNNNGC 2 cut(s) 143, 168
BstSFI CTRYAG 1 cut(s) 147
BstV1I GCAGC 5 cut(s) 55, 73, 133, 155, 161
Cac8I GCNNGC 1 cut(s) 213
CaiI CAGNNNCTG 1 cut(s) 83
CseI GACGC 1 cut(s) 217
CviJI RGCY 4 cut(s) 35, 168, 218, 253
CviKI_1 RGCY 4 cut(s) 35, 168, 218, 253
DpnI GATC 1 cut(s) 143
DpnII GATC 1 cut(s) 141
Eco130I CCWWGG 1 cut(s) 172
EcoT14I CCWWGG 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 172
FaiI YATR 5 cut(s) 12, 131, 231, 233, 235
FaqI GGGAC 1 cut(s) 65
FauI CCCGC 1 cut(s) 155
Fnu4HI GCNGC 6 cut(s) 69, 87, 147, 150, 169, 216
Fsp4HI GCNGC 6 cut(s) 69, 87, 147, 150, 169, 216
GluI GCNGC 6 cut(s) 69, 87, 147, 150, 169, 216
HgaI GACGC 1 cut(s) 217
HphI GGTGA 2 cut(s) 10, 19
HpyAV CCTTC 5 cut(s) 31, 59, 73, 152, 178
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4V TGCA 1 cut(s) 149
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 168
Kzo9I GATC 1 cut(s) 141
LmnI GCTCC 1 cut(s) 165
LpnPI CCDG 6 cut(s) 90, 96, 110, 197, 209, 254
Lsp1109I GCAGC 5 cut(s) 55, 73, 133, 155, 161
MalI GATC 1 cut(s) 143
MboI GATC 1 cut(s) 141
MboII GAAGA 1 cut(s) 16
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 6 cut(s) 50, 53, 148, 181, 195, 198
MspA1I CMGCKG 1 cut(s) 152
MwoI GCNNNNNNNGC 2 cut(s) 143, 168
NdeII GATC 1 cut(s) 141
PkrI GCNGC 6 cut(s) 70, 88, 148, 151, 170, 217
PstI CTGCAG 1 cut(s) 151
PstNI CAGNNNCTG 1 cut(s) 83
SatI GCNGC 6 cut(s) 69, 87, 147, 150, 169, 216
Sau3AI GATC 1 cut(s) 141
SetI ASST 5 cut(s) 23, 37, 51, 65, 170
SfcI CTRYAG 1 cut(s) 147
SgeI CNNG 9 cut(s) 84, 89, 95, 137, 175, 185, 208, 224, 253
SsiI CCGC 3 cut(s) 152, 162, 215
StyI CCWWGG 1 cut(s) 172
TaaI ACNGT 1 cut(s) 31
TauI GCSGC 1 cut(s) 218
TseI GCWGC 5 cut(s) 68, 86, 146, 149, 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.