MD10G1110000.v1.1

Phytosulfokines

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003745
Physical Location & Seq
Reverse (-)
18087718 .. 18088297
580 bp
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UTR
Exon/CDS
Intron
MD10G1110000.v1.1.491

Sequence Viewer

Length: 258 bp
ATGTCGTCCATGCTCACCAGCCTCTGTGTGGTTGTTCTCCTTCTCTTCCCCTCATTATCCGGCATGGCGGCACGGCCGTTGCCTTCTTCTTCTTCTGATGTCAGTGCGGTGAAAGTTCAACATGAGTTGGAGGATGTTGAGGTAGAAAAATCCATGGTGGAGGAAAGTTGTGAAGGAGTTGGGGAAGATGAGTGCCTAACGAGAAGAACTCTTGCGGCTCATATTGATTACATCTATACGCAGAAGCACAGCCCATAA

Protein Analysis

86

Amino Acids

9.27

Weight (kDa)

4.68

Isoelectric Point (pI)

68.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSK PF06404 13 - 84 7.1e-17 Phytosulfokine precursor protein (PSK)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 68, 107, 215
AcoI YGGCCR 1 cut(s) 74
AfiI CCNNNNNNNGG 1 cut(s) 28
AgsI TTSAA 1 cut(s) 119
AlwNI CAGNNNCTG 1 cut(s) 24
AoxI GGCC 1 cut(s) 74
AsuHPI GGTGA 2 cut(s) 7, 121
BceAI ACGGC 2 cut(s) 61, 89
BisI GCNGC 2 cut(s) 69, 216
BlsI GCNGC 2 cut(s) 70, 217
BplI GAGNNNNNCTC 2 cut(s) 193, 225
BsaJI CCNNGG 1 cut(s) 153
Bsc4I CCNNNNNNNGG 1 cut(s) 28
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 1 cut(s) 139
BseLI CCNNNNNNNGG 1 cut(s) 28
BseX3I CGGCCG 1 cut(s) 74
Bsh1285I CGRYCG 1 cut(s) 77
BshFI GGCC 1 cut(s) 76
BsiEI CGRYCG 1 cut(s) 77
BsiSI CCGG 1 cut(s) 60
BslI CCNNNNNNNGG 1 cut(s) 28
BsnI GGCC 1 cut(s) 76
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 3 cut(s) 68, 107, 215
BspANI GGCC 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 153
BssT1I CCWWGG 1 cut(s) 153
Bst6I CTCTTC 1 cut(s) 50
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 139
BstMCI CGRYCG 1 cut(s) 77
BstZI CGGCCG 1 cut(s) 74
BsuRI GGCC 1 cut(s) 76
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 139
BtsIMutI CAGTG 1 cut(s) 109
CaiI CAGNNNCTG 1 cut(s) 24
CviAII CATG 4 cut(s) 10, 64, 122, 154
CviJI RGCY 4 cut(s) 21, 76, 218, 252
CviKI_1 RGCY 4 cut(s) 21, 76, 218, 252
EaeI YGGCCR 1 cut(s) 74
EagI CGGCCG 1 cut(s) 74
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
EclXI CGGCCG 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 153
Eco52I CGGCCG 1 cut(s) 74
EcoT14I CCWWGG 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 4 cut(s) 13, 67, 125, 157
FaiI YATR 7 cut(s) 11, 65, 123, 155, 222, 237, 256
FatI CATG 4 cut(s) 9, 63, 121, 153
Fnu4HI GCNGC 2 cut(s) 69, 216
FokI GGATG 1 cut(s) 146
Fsp4HI GCNGC 2 cut(s) 69, 216
GluI GCNGC 2 cut(s) 69, 216
HaeIII GGCC 1 cut(s) 76
HapII CCGG 1 cut(s) 60
Hin1II CATG 4 cut(s) 13, 67, 125, 157
HpaII CCGG 1 cut(s) 60
HphI GGTGA 2 cut(s) 7, 121
Hpy188I TCNGA 1 cut(s) 97
HpyAV CCTTC 3 cut(s) 50, 93, 167
Hsp92II CATG 4 cut(s) 13, 67, 125, 157
LpnPI CCDG 2 cut(s) 31, 73
MboII GAAGA 6 cut(s) 37, 78, 81, 84, 197, 216
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 5 cut(s) 32, 61, 124, 133, 154
MspI CCGG 1 cut(s) 60
NcoI CCATGG 1 cut(s) 153
NlaIII CATG 4 cut(s) 13, 67, 125, 157
PkrI GCNGC 2 cut(s) 70, 217
PstNI CAGNNNCTG 1 cut(s) 24
SatI GCNGC 2 cut(s) 69, 216
SetI ASST 1 cut(s) 144
SgeI CNNG 9 cut(s) 22, 30, 72, 76, 84, 134, 166, 213, 224
SsiI CCGC 3 cut(s) 68, 107, 215
StyI CCWWGG 1 cut(s) 153
TauI GCSGC 2 cut(s) 71, 218
TscAI CASTG 1 cut(s) 109
TspRI CASTG 1 cut(s) 109
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.