MD15G1066300.v1.1

Phytosulfokines

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003745
Physical Location & Seq
Forward (+)
4612903 .. 4613689
787 bp
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UTR
Exon/CDS
Intron
MD15G1066300.v1.1.491

Sequence Viewer

Length: 255 bp
ATGGCTAAATATATTATCACCTTCTTCACCATAGTTCTGCTTCTCAGTTTTCAACTCACAGCCTTCGCTGCTCGTCCAGTCCCTGCTGCTTTCACCAACAACGCTCTCAAAACCCAACACCAAGACATAGACGCGGAGATCGTTGCAGCGGAGGGAGGCGGGAGCTGCGAAGGAGTAGCGGAGGAGGAGTGCCTGATGAGGAGGACATTGGCGGCTCATGTGGATTACATATATACACAGAAGCACAAGCCTTGA

Protein Analysis

85

Amino Acids

9.26

Weight (kDa)

5.82

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSK PF06404 13 - 84 1.4e-15 Phytosulfokine precursor protein (PSK)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 134
AciI CCGC 5 cut(s) 134, 149, 159, 179, 212
AgsI TTSAA 1 cut(s) 53
AluBI AGCT 1 cut(s) 165
AluI AGCT 1 cut(s) 165
AlwNI CAGNNNCTG 1 cut(s) 83
ApeKI GCWGC 4 cut(s) 68, 86, 146, 165
AsuHPI GGTGA 3 cut(s) 10, 19, 85
BbvI GCAGC 4 cut(s) 55, 73, 152, 158
BisI GCNGC 5 cut(s) 69, 87, 147, 166, 213
BlsI GCNGC 5 cut(s) 70, 88, 148, 167, 214
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bse1I ACTGG 1 cut(s) 77
BseMII CTCAG 1 cut(s) 58
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 3 cut(s) 197, 200, 214
BseXI GCAGC 4 cut(s) 55, 73, 152, 158
Bsh1236I CGCG 1 cut(s) 134
BslFI GGGAC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 65
Bsp143I GATC 1 cut(s) 138
BspACI CCGC 5 cut(s) 134, 149, 159, 179, 212
BspCNI CTCAG 1 cut(s) 57
BspFNI CGCG 1 cut(s) 134
BsrI ACTGG 1 cut(s) 77
BssMI GATC 1 cut(s) 138
BstDEI CTNAG 1 cut(s) 44
BstFNI CGCG 1 cut(s) 134
BstKTI GATC 1 cut(s) 141
BstMBI GATC 1 cut(s) 138
BstMWI GCNNNNNNNGC 2 cut(s) 68, 165
BstUI CGCG 1 cut(s) 134
BstV1I GCAGC 4 cut(s) 55, 73, 152, 158
CaiI CAGNNNCTG 1 cut(s) 83
CseI GACGC 1 cut(s) 140
CviAII CATG 1 cut(s) 218
CviJI RGCY 5 cut(s) 5, 62, 165, 215, 250
CviKI_1 RGCY 5 cut(s) 5, 62, 165, 215, 250
DdeI CTNAG 1 cut(s) 44
DpnI GATC 1 cut(s) 140
DpnII GATC 1 cut(s) 138
FaeI CATG 1 cut(s) 221
FaiI YATR 7 cut(s) 12, 32, 128, 219, 230, 232, 234
FaqI GGGAC 1 cut(s) 65
FatI CATG 1 cut(s) 217
FauI CCCGC 1 cut(s) 152
Fnu4HI GCNGC 5 cut(s) 69, 87, 147, 166, 213
Fsp4HI GCNGC 5 cut(s) 69, 87, 147, 166, 213
GluI GCNGC 5 cut(s) 69, 87, 147, 166, 213
HgaI GACGC 1 cut(s) 140
Hin1II CATG 1 cut(s) 221
HphI GGTGA 3 cut(s) 10, 19, 85
HpyAV CCTTC 3 cut(s) 31, 73, 164
HpyCH4V TGCA 1 cut(s) 146
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 165
HpyF3I CTNAG 1 cut(s) 44
Hsp92II CATG 1 cut(s) 221
Kzo9I GATC 1 cut(s) 138
LmnI GCTCC 1 cut(s) 162
LpnPI CCDG 3 cut(s) 90, 96, 206
Lsp1109I GCAGC 4 cut(s) 55, 73, 152, 158
MalI GATC 1 cut(s) 140
MboI GATC 1 cut(s) 138
MboII GAAGA 1 cut(s) 16
MnlI CCTC 6 cut(s) 145, 149, 175, 178, 192, 195
MspA1I CMGCKG 1 cut(s) 149
MvnI CGCG 1 cut(s) 134
MwoI GCNNNNNNNGC 2 cut(s) 68, 165
NdeII GATC 1 cut(s) 138
NlaIII CATG 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PkrI GCNGC 5 cut(s) 70, 88, 148, 167, 214
PstNI CAGNNNCTG 1 cut(s) 83
SatI GCNGC 5 cut(s) 69, 87, 147, 166, 213
Sau3AI GATC 1 cut(s) 138
SetI ASST 2 cut(s) 23, 167
SgeI CNNG 8 cut(s) 84, 89, 95, 134, 145, 172, 205, 230
SsiI CCGC 5 cut(s) 134, 149, 159, 179, 212
TauI GCSGC 1 cut(s) 215
TseI GCWGC 4 cut(s) 68, 86, 146, 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.