FvH4_2g32930

NADH dehydrogenase ubiquinone iron-sulfur protein 4, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002607
Physical Location & Seq
Reverse (-)
24837765 .. 24838839
1075 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32930.t1

Sequence Viewer

Length: 468 bp
ATGGCGAGCTCTCTACGACACGGATTCATCAAAGCCCGATCACCTCTTCTCTCCCGGACGAGATGGTTCTCCTCGTCGCAATCTCAGGCGGTGGTGGAGACCAAGATTCCCGACGTCGGCATCATCTCTGGAATCCCCGAACAGCATCTCAAAAGAAGGGTCGTAATATATTCTCCTGCTCGAACTGCATCACAGCAAGGTTCTGGGAAAGTTGGGAAATGGAAAATTAATTTCCTTTCTACGCAGAAGTGGGAAAATCCATTGATGGGTTGGACTTCCACTGGTGATCCCTATGCCAATGTGGGTGATGCAGCACTGAGTTTCGATAGTGAACAAGCTGCAAAGGAGTTTGCTGAGAAACATGGCTGGGATTATACGGTCAGGAAGCGTCACACACCACTTCTAAGGGTCAGGACTTATGCAGACAACTTCAAGTGGAAGGGGCTGCCAAAGAGCGAGGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
ETC_C1_NDUFA4

Protein Analysis

156

Amino Acids

17.53

Weight (kDa)

10.08

Isoelectric Point (pI)

49.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUS4 PF04800 53 - 147 2e-30 NADH dehydrogenase ubiquinone Fe-S protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 117
AciI CCGC 1 cut(s) 89
AclWI GGATC 1 cut(s) 281
AcyI GRCGYC 1 cut(s) 114
AfiI CCNNNNNNNGG 2 cut(s) 116, 266
AgsI TTSAA 1 cut(s) 433
AluBI AGCT 2 cut(s) 9, 338
AluI AGCT 2 cut(s) 9, 338
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 1 cut(s) 281
ApeKI GCWGC 3 cut(s) 311, 338, 445
ArsI GACNNNNNNTTYG 2 cut(s) 144, 176
AseI ATTAAT 1 cut(s) 228
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 3 cut(s) 33, 296, 317
BanII GRGCYC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 3 cut(s) 323, 325, 432
BccI CCATC 2 cut(s) 57, 259
BcnI CCSGG 1 cut(s) 55
BcoDI GTCTC 1 cut(s) 92
BisI GCNGC 3 cut(s) 312, 339, 446
BlsI GCNGC 3 cut(s) 313, 340, 447
Bme1390I CCNGG 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 55
BmsI GCATC 4 cut(s) 129, 154, 197, 298
BpuMI CCSGG 1 cut(s) 55
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 92
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 266
Bse1I ACTGG 1 cut(s) 286
BseLI CCNNNNNNNGG 2 cut(s) 116, 266
BseMII CTCAG 3 cut(s) 98, 308, 345
BseNI ACTGG 1 cut(s) 286
BseRI GAGGAG 1 cut(s) 61
BseXI GCAGC 3 cut(s) 323, 325, 432
BseYI CCCAGC 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 11
BsiSI CCGG 1 cut(s) 55
BslI CCNNNNNNNGG 2 cut(s) 116, 266
BsmAI GTCTC 1 cut(s) 92
Bso31I GGTCTC 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 2 cut(s) 38, 286
BspACI CCGC 1 cut(s) 89
BspCNI CTCAG 3 cut(s) 97, 309, 346
BspPI GGATC 1 cut(s) 281
BspTNI GGTCTC 1 cut(s) 92
BsrI ACTGG 1 cut(s) 286
BssMI GATC 2 cut(s) 38, 286
BssNI GRCGYC 1 cut(s) 114
Bst4CI ACNGT 1 cut(s) 379
Bst6I CTCTTC 1 cut(s) 51
BstACI GRCGYC 1 cut(s) 114
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 4 cut(s) 84, 317, 354, 404
BstKTI GATC 2 cut(s) 41, 289
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 2 cut(s) 38, 286
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 53
BstV1I GCAGC 3 cut(s) 323, 325, 432
BtsIMutI CAGTG 2 cut(s) 279, 314
Cac8I GCNNGC 1 cut(s) 7
CseI GACGC 1 cut(s) 377
CviAII CATG 1 cut(s) 362
CviJI RGCY 5 cut(s) 9, 35, 338, 366, 445
CviKI_1 RGCY 5 cut(s) 9, 35, 338, 366, 445
DdeI CTNAG 4 cut(s) 84, 317, 354, 404
DpnI GATC 2 cut(s) 40, 288
DpnII GATC 2 cut(s) 38, 286
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Ecl136II GAGCTC 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 11
Eco31I GGTCTC 1 cut(s) 92
Eco53kI GAGCTC 1 cut(s) 9
EcoICRI GAGCTC 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 11
FaeI CATG 1 cut(s) 365
FaiI YATR 5 cut(s) 169, 294, 363, 375, 420
FatI CATG 1 cut(s) 361
Fnu4HI GCNGC 3 cut(s) 312, 339, 446
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 3 cut(s) 312, 339, 446
GluI GCNGC 3 cut(s) 312, 339, 446
GsaI CCCAGC 1 cut(s) 370
HapII CCGG 1 cut(s) 55
HgaI GACGC 1 cut(s) 377
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 1 cut(s) 365
HinfI GANTC 3 cut(s) 24, 106, 132
HpaII CCGG 1 cut(s) 55
HphI GGTGA 3 cut(s) 33, 296, 317
Hpy166II GTNNAC 1 cut(s) 332
Hpy188III TCNNGA 4 cut(s) 110, 129, 382, 412
Hpy8I GTNNAC 1 cut(s) 332
Hpy99I CGWCG 3 cut(s) 79, 116, 119
HpyAV CCTTC 2 cut(s) 150, 433
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 4 cut(s) 188, 311, 341, 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpyF3I CTNAG 4 cut(s) 84, 317, 354, 404
HpySE526I ACGT 1 cut(s) 114
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 1 cut(s) 365
Kzo9I GATC 2 cut(s) 38, 286
LpnPI CCDG 9 cut(s) 68, 71, 114, 189, 189, 267, 352, 367, 397
Lsp1109I GCAGC 3 cut(s) 323, 325, 432
LweI GCATC 4 cut(s) 129, 154, 197, 298
MaeII ACGT 1 cut(s) 114
MaeIII GTNAC 1 cut(s) 389
MalI GATC 2 cut(s) 40, 288
MboI GATC 2 cut(s) 38, 286
MboII GAAGA 1 cut(s) 38
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 2 cut(s) 225, 229
MmeI TCCRAC 1 cut(s) 251
MnlI CCTC 3 cut(s) 54, 82, 451
MseI TTAA 1 cut(s) 228
MspI CCGG 1 cut(s) 55
MspR9I CCNGG 1 cut(s) 55
MwoI GCNNNNNNNGC 1 cut(s) 185
NciI CCSGG 1 cut(s) 55
NdeII GATC 2 cut(s) 38, 286
NlaIII CATG 1 cut(s) 365
NmuCI GTSAC 1 cut(s) 389
PfeI GAWTC 3 cut(s) 24, 106, 132
PfoI TCCNGGA 1 cut(s) 53
PkrI GCNGC 3 cut(s) 313, 340, 447
PshBI ATTAAT 1 cut(s) 228
Psp124BI GAGCTC 1 cut(s) 11
PspFI CCCAGC 1 cut(s) 366
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 3 cut(s) 312, 339, 446
Sau3AI GATC 2 cut(s) 38, 286
ScrFI CCNGG 1 cut(s) 55
SduI GDGCHC 1 cut(s) 11
SetI ASST 5 cut(s) 11, 46, 117, 202, 340
SfaNI GCATC 4 cut(s) 129, 154, 197, 298
Sse9I AATT 2 cut(s) 225, 229
SsiI CCGC 1 cut(s) 89
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 53
TaaI ACNGT 1 cut(s) 379
TaiI ACGT 1 cut(s) 117
TaqI TCGA 2 cut(s) 181, 324
TasI AATT 2 cut(s) 225, 229
TfiI GAWTC 3 cut(s) 24, 106, 132
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 2 cut(s) 286, 321
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 3 cut(s) 311, 338, 445
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 1 cut(s) 16
TspGWI ACGGA 1 cut(s) 36
TspRI CASTG 2 cut(s) 286, 321
VspI ATTAAT 1 cut(s) 228
XcmI CCANNNNNNNNNTGG 1 cut(s) 267
ZraI GACGTC 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.