Rorug06G0374300

NADH dehydrogenase ubiquinone iron-sulfur protein 4, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002607
Physical Location & Seq
Reverse (-)
52027996 .. 52028549
554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0374300.1

Sequence Viewer

Length: 423 bp
ATGTATCGTAAGGATGTGTATGTTTGGTGGGTGGTAGAGATAGTAACTTTTCATTTAGGAGGTCACGTATTCAAGTCCCATCAAGAGTGCGATGGGGTTGAGCTCTTTGCTCTGCAAGCTGCAGTTCAGTTTGCGATATCTTTGCACCATGAGAAGGTTCAATTTGAGACAGATTGCCTCCAACTCGTGCAAGTTATTCATGCTGAGGATGAAGATGCTTCAGTTGTTGGCCATATTGTGGAGGAGGTTCGTGGGCTGTTTCATAATTATCCTTTCTTTTCTCTAGCTCATGTTAATCGTCGAGCGAATAATGTAGCTCATATTCTTGCTCAGACGGCTCTTCACTCTGAGCTGTCTCATACTTGGTTCTTGTATGCTCCCGAGTTTATACGGGATGCCATCTTAAGCGATATTCATCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
ETC_C1_NDUFA4

Protein Analysis

140

Amino Acids

16.2

Weight (kDa)

5.73

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 23 - 113 6.9e-16 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 238
AcoI YGGCCR 1 cut(s) 229
AcuI CTGAAG 1 cut(s) 204
AfiI CCNNNNNNNGG 2 cut(s) 154, 238
AflII CTTAAG 1 cut(s) 403
AgsI TTSAA 2 cut(s) 73, 161
AluBI AGCT 5 cut(s) 103, 119, 287, 317, 352
AluI AGCT 5 cut(s) 103, 119, 287, 317, 352
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 2 cut(s) 161, 360
Ama87I CYCGRG 1 cut(s) 380
AoxI GGCC 1 cut(s) 229
ApeKI GCWGC 1 cut(s) 119
AvaI CYCGRG 1 cut(s) 380
BalI TGGCCA 1 cut(s) 231
BanII GRGCYC 1 cut(s) 105
BauI CACGAG 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 105
BbvCI CCTCAGC 1 cut(s) 204
BbvI GCAGC 1 cut(s) 106
BccI CCATC 3 cut(s) 86, 87, 407
BceAI ACGGC 1 cut(s) 351
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BcoDI GTCTC 2 cut(s) 161, 360
BfaI CTAG 1 cut(s) 284
BfmI CTRYAG 1 cut(s) 120
BfrI CTTAAG 1 cut(s) 403
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 380
BmsI GCATC 2 cut(s) 205, 385
Bpu10I CCTNAGC 1 cut(s) 204
BsaAI YACGTR 1 cut(s) 67
BsaBI GATNNNNATC 1 cut(s) 414
BsaXI ACNNNNNCTCC 2 cut(s) 51, 81
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 238
Bse8I GATNNNNATC 1 cut(s) 414
BseGI GGATG 3 cut(s) 19, 214, 400
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 2 cut(s) 154, 238
BseMII CTCAG 3 cut(s) 195, 339, 344
BseRI GAGGAG 1 cut(s) 257
BseXI GCAGC 1 cut(s) 106
BshFI GGCC 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 380
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 2 cut(s) 154, 238
BsmAI GTCTC 2 cut(s) 161, 360
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 1 cut(s) 231
BsoBI CYCGRG 1 cut(s) 380
Bsp1286I GDGCHC 1 cut(s) 105
BspANI GGCC 1 cut(s) 231
BspCNI CTCAG 3 cut(s) 196, 340, 343
BspMAI CTGCAG 1 cut(s) 124
BspQI GCTCTTC 1 cut(s) 345
BspTI CTTAAG 1 cut(s) 403
BssSI CACGAG 1 cut(s) 185
Bst2BI CACGAG 1 cut(s) 185
Bst6I CTCTTC 1 cut(s) 345
BstAFI CTTAAG 1 cut(s) 403
BstBAI YACGTR 1 cut(s) 67
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 3 cut(s) 204, 330, 348
BstF5I GGATG 3 cut(s) 19, 214, 400
BstMAI GTCTC 2 cut(s) 161, 360
BstMWI GCNNNNNNNGC 2 cut(s) 116, 335
BstSFI CTRYAG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 106
BsuRI GGCC 1 cut(s) 231
BtgZI GCGATG 2 cut(s) 105, 401
BtsCI GGATG 3 cut(s) 19, 214, 400
Cac8I GCNNGC 1 cut(s) 117
CviAII CATG 3 cut(s) 149, 200, 290
CviJI RGCY 8 cut(s) 103, 119, 231, 256, 287, 317, 338, 352
CviKI_1 RGCY 8 cut(s) 103, 119, 231, 256, 287, 317, 338, 352
DdeI CTNAG 3 cut(s) 204, 330, 348
EaeI YGGCCR 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
Ecl136II GAGCTC 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 105
Eco32I GATATC 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 380
EcoICRI GAGCTC 1 cut(s) 103
EcoRV GATATC 1 cut(s) 138
EcoT38I GRGCYC 1 cut(s) 105
FaeI CATG 3 cut(s) 152, 203, 293
FaqI GGGAC 1 cut(s) 61
FatI CATG 3 cut(s) 148, 199, 289
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 3 cut(s) 26, 221, 407
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 1 cut(s) 120
FspBI CTAG 1 cut(s) 284
GluI GCNGC 1 cut(s) 120
HaeIII GGCC 1 cut(s) 231
Hin1II CATG 3 cut(s) 152, 203, 293
Hpy188I TCNGA 2 cut(s) 333, 349
Hpy188III TCNNGA 2 cut(s) 83, 380
Hpy99I CGWCG 1 cut(s) 303
HpyAV CCTTC 1 cut(s) 148
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 4 cut(s) 115, 122, 145, 190
HpyF10VI GCNNNNNNNGC 2 cut(s) 116, 335
HpyF3I CTNAG 3 cut(s) 204, 330, 348
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 3 cut(s) 152, 203, 293
LguI GCTCTTC 1 cut(s) 345
LmnI GCTCC 1 cut(s) 382
Lsp1109I GCAGC 1 cut(s) 106
LweI GCATC 2 cut(s) 205, 385
MaeI CTAG 1 cut(s) 284
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 2 cut(s) 43, 62
MboII GAAGA 2 cut(s) 224, 332
MhlI GDGCHC 1 cut(s) 105
MlsI TGGCCA 1 cut(s) 231
MluCI AATT 2 cut(s) 161, 265
MluNI TGGCCA 1 cut(s) 231
MmeI TCCRAC 1 cut(s) 205
MnlI CCTC 5 cut(s) 53, 188, 199, 235, 238
Mox20I TGGCCA 1 cut(s) 231
MscI TGGCCA 1 cut(s) 231
MseI TTAA 2 cut(s) 294, 404
Msp20I TGGCCA 1 cut(s) 231
MspCI CTTAAG 1 cut(s) 403
MwoI GCNNNNNNNGC 2 cut(s) 116, 335
NlaIII CATG 3 cut(s) 152, 203, 293
NmuCI GTSAC 1 cut(s) 62
PciSI GCTCTTC 1 cut(s) 345
PflMI CCANNNNNTGG 1 cut(s) 238
PkrI GCNGC 1 cut(s) 121
Ppu21I YACGTR 1 cut(s) 67
Psp124BI GAGCTC 1 cut(s) 105
PstI CTGCAG 1 cut(s) 124
SacI GAGCTC 1 cut(s) 105
SapI GCTCTTC 1 cut(s) 345
SaqAI TTAA 2 cut(s) 294, 404
SatI GCNGC 1 cut(s) 120
SduI GDGCHC 1 cut(s) 105
SetI ASST 9 cut(s) 64, 69, 105, 121, 159, 249, 289, 319, 354
SfaNI GCATC 2 cut(s) 205, 385
SfcI CTRYAG 1 cut(s) 120
SmlI CTYRAG 1 cut(s) 403
SmoI CTYRAG 1 cut(s) 403
Sse9I AATT 2 cut(s) 161, 265
SspMI CTAG 1 cut(s) 284
SstI GAGCTC 1 cut(s) 105
TaiI ACGT 1 cut(s) 69
TaqI TCGA 1 cut(s) 301
TasI AATT 2 cut(s) 161, 265
Tru1I TTAA 2 cut(s) 294, 404
Tru9I TTAA 2 cut(s) 294, 404
TseFI GTSAC 1 cut(s) 62
TseI GCWGC 1 cut(s) 119
Tsp45I GTSAC 1 cut(s) 62
TspDTI ATGAA 5 cut(s) 41, 188, 225, 251, 404
Van91I CCANNNNNTGG 1 cut(s) 238
Vha464I CTTAAG 1 cut(s) 403
XspI CTAG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.