MD15G1067900.v1.1

NADH dehydrogenase ubiquinone iron-sulfur protein 4, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002607
Physical Location & Seq
Reverse (-)
4712079 .. 4715213
3135 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1067900.v1.1.491

Sequence Viewer

Length: 348 bp
ATGGCGCGACCTCTACGACACGGCTTCAACGTCGCCCGAGCGCACGTGCTTTCGCGGCCGCGATGGGCGTCGTCGCAGTCAGCGGAGGCACTGGTGGAGGTCAAAGTCTCCGACATAGGCATGATATCGGGAATCCCCGAACAGCATCTCCGACGAAGGGTTATAATCTATTCGCCTGCTCGAACTGCGACCCAACAAGGTTCTGGAAAAGTCGGGAAATGGAAAATTAATTTTCTTTCGACGCAAAAGAAGCGTCACACACCACTTTTGAAGGCATATGCAGACAACTTCAAGTGGAAGGGCCCTGATAAGAGTGAGAAGACTGCAGCTGATGCTGTAAACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
ETC_C1_NDUFA4

Protein Analysis

116

Amino Acids

12.86

Weight (kDa)

10.84

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUS4 PF04800 53 - 90 1.8e-06 NADH dehydrogenase ubiquinone Fe-S protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 164
AccII CGCG 3 cut(s) 7, 55, 61
AciI CCGC 3 cut(s) 55, 59, 83
AcoI YGGCCR 1 cut(s) 56
AcvI CACGTG 1 cut(s) 46
AcyI GRCGYC 1 cut(s) 68
AfiI CCNNNNNNNGG 1 cut(s) 157
AgsI TTSAA 3 cut(s) 28, 271, 292
AloI GAACNNNNNNTCC 2 cut(s) 132, 164
AluBI AGCT 1 cut(s) 329
AluI AGCT 1 cut(s) 329
Alw26I GTCTC 1 cut(s) 112
Ama87I CYCGRG 1 cut(s) 36
AoxI GGCC 2 cut(s) 56, 301
ApaI GGGCCC 1 cut(s) 305
ApeKI GCWGC 1 cut(s) 326
ArsI GACNNNNNNTTYG 2 cut(s) 238, 270
AseI ATTAAT 1 cut(s) 228
AspLEI GCGC 2 cut(s) 7, 43
AspS9I GGNCC 2 cut(s) 301, 302
AvaI CYCGRG 1 cut(s) 36
BaeGI GKGCMC 1 cut(s) 305
BanII GRGCYC 1 cut(s) 305
BbrPI CACGTG 1 cut(s) 46
BbsI GAAGAC 1 cut(s) 326
BbvI GCAGC 1 cut(s) 338
BccI CCATC 1 cut(s) 57
BceAI ACGGC 1 cut(s) 37
BcoDI GTCTC 1 cut(s) 112
BfmI CTRYAG 1 cut(s) 324
BisI GCNGC 3 cut(s) 56, 59, 327
BlsI GCNGC 3 cut(s) 57, 60, 328
BmeT110I CYCGRG 1 cut(s) 36
BmgT120I GGNCC 2 cut(s) 301, 302
BmiI GGNNCC 1 cut(s) 303
BmsI GCATC 2 cut(s) 154, 322
BpiI GAAGAC 1 cut(s) 326
BsaAI YACGTR 1 cut(s) 46
BsaHI GRCGYC 1 cut(s) 68
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 96
BseLI CCNNNNNNNGG 1 cut(s) 157
BseNI ACTGG 1 cut(s) 96
BseSI GKGCMC 1 cut(s) 305
BseX3I CGGCCG 1 cut(s) 56
BseXI GCAGC 1 cut(s) 338
Bsh1236I CGCG 3 cut(s) 7, 55, 61
Bsh1285I CGRYCG 1 cut(s) 59
BshFI GGCC 2 cut(s) 58, 303
BsiEI CGRYCG 1 cut(s) 59
BsiHKCI CYCGRG 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 1 cut(s) 112
BsnI GGCC 2 cut(s) 58, 303
BsoBI CYCGRG 1 cut(s) 36
Bsp120I GGGCCC 1 cut(s) 301
Bsp1286I GDGCHC 1 cut(s) 305
BspACI CCGC 3 cut(s) 55, 59, 83
BspANI GGCC 2 cut(s) 58, 303
BspFNI CGCG 3 cut(s) 7, 55, 61
BspLI GGNNCC 1 cut(s) 303
BspMAI CTGCAG 1 cut(s) 328
BsrI ACTGG 1 cut(s) 96
BssNI GRCGYC 1 cut(s) 68
BstACI GRCGYC 1 cut(s) 68
BstAPI GCANNNNNTGC 1 cut(s) 332
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 1 cut(s) 177
BstFNI CGCG 3 cut(s) 7, 55, 61
BstHHI GCGC 2 cut(s) 7, 43
BstMAI GTCTC 1 cut(s) 112
BstMCI CGRYCG 1 cut(s) 59
BstMWI GCNNNNNNNGC 4 cut(s) 55, 185, 250, 332
BstSFI CTRYAG 1 cut(s) 324
BstSLI GKGCMC 1 cut(s) 305
BstUI CGCG 3 cut(s) 7, 55, 61
BstV1I GCAGC 1 cut(s) 338
BstV2I GAAGAC 1 cut(s) 326
BstZI CGGCCG 1 cut(s) 56
BsuRI GGCC 2 cut(s) 58, 303
BtgZI GCGATG 1 cut(s) 76
BtsIMutI CAGTG 1 cut(s) 89
Cac8I GCNNGC 1 cut(s) 177
CciNI GCGGCCGC 1 cut(s) 56
CfoI GCGC 2 cut(s) 7, 43
Cfr13I GGNCC 2 cut(s) 301, 302
CseI GACGC 3 cut(s) 57, 242, 250
CviAII CATG 1 cut(s) 121
CviJI RGCY 4 cut(s) 24, 58, 303, 329
CviKI_1 RGCY 4 cut(s) 24, 58, 303, 329
EaeI YGGCCR 1 cut(s) 56
EagI CGGCCG 1 cut(s) 56
EclXI CGGCCG 1 cut(s) 56
Eco24I GRGCYC 1 cut(s) 305
Eco32I GATATC 1 cut(s) 126
Eco52I CGGCCG 1 cut(s) 56
Eco72I CACGTG 1 cut(s) 46
Eco88I CYCGRG 1 cut(s) 36
EcoO109I RGGNCCY 2 cut(s) 301, 302
EcoRV GATATC 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 305
FaeI CATG 1 cut(s) 124
FaiI YATR 5 cut(s) 116, 122, 164, 277, 279
FatI CATG 1 cut(s) 120
FauNDI CATATG 1 cut(s) 277
Fnu4HI GCNGC 3 cut(s) 56, 59, 327
FriOI GRGCYC 1 cut(s) 305
Fsp4HI GCNGC 3 cut(s) 56, 59, 327
GlaI GCGC 2 cut(s) 6, 42
GluI GCNGC 3 cut(s) 56, 59, 327
HaeIII GGCC 2 cut(s) 58, 303
HgaI GACGC 3 cut(s) 57, 242, 250
HhaI GCGC 2 cut(s) 7, 43
Hin1I GRCGYC 1 cut(s) 68
Hin1II CATG 1 cut(s) 124
Hin6I GCGC 2 cut(s) 5, 41
HinP1I GCGC 2 cut(s) 5, 41
HinfI GANTC 1 cut(s) 132
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 3 cut(s) 112, 152, 347
Hpy188III TCNNGA 3 cut(s) 129, 204, 214
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 5 cut(s) 35, 73, 76, 156, 244
HpyAV CCTTC 3 cut(s) 150, 265, 292
HpyCH4IV ACGT 2 cut(s) 30, 45
HpyCH4V TGCA 2 cut(s) 281, 326
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 185, 250, 332
HpySE526I ACGT 2 cut(s) 30, 45
Hsp92I GRCGYC 1 cut(s) 68
Hsp92II CATG 1 cut(s) 124
HspAI GCGC 2 cut(s) 5, 41
LpnPI CCDG 4 cut(s) 77, 189, 189, 318
Lsp1109I GCAGC 1 cut(s) 338
LweI GCATC 2 cut(s) 154, 322
MaeII ACGT 2 cut(s) 30, 45
MaeIII GTNAC 1 cut(s) 254
MboII GAAGA 1 cut(s) 331
MhlI GDGCHC 1 cut(s) 305
MluCI AATT 2 cut(s) 225, 229
MmeI TCCRAC 2 cut(s) 135, 175
MnlI CCTC 3 cut(s) 21, 79, 91
MseI TTAA 1 cut(s) 228
MslI CAYNNNNRTG 1 cut(s) 119
MspA1I CMGCKG 2 cut(s) 83, 329
MvnI CGCG 3 cut(s) 7, 55, 61
MwoI GCNNNNNNNGC 4 cut(s) 55, 185, 250, 332
NdeI CATATG 1 cut(s) 277
NlaIII CATG 1 cut(s) 124
NlaIV GGNNCC 1 cut(s) 303
NmuCI GTSAC 1 cut(s) 254
NotI GCGGCCGC 1 cut(s) 56
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PfeI GAWTC 1 cut(s) 132
PkrI GCNGC 3 cut(s) 57, 60, 328
PmaCI CACGTG 1 cut(s) 46
PmlI CACGTG 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 46
PshBI ATTAAT 1 cut(s) 228
PsiI TTATAA 1 cut(s) 164
PspCI CACGTG 1 cut(s) 46
PspN4I GGNNCC 1 cut(s) 303
PspOMI GGGCCC 1 cut(s) 301
PspPI GGNCC 2 cut(s) 301, 302
PstI CTGCAG 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 329
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 3 cut(s) 56, 59, 327
Sau96I GGNCC 2 cut(s) 301, 302
SduI GDGCHC 1 cut(s) 305
SetI ASST 6 cut(s) 13, 33, 48, 102, 202, 331
SfaNI GCATC 2 cut(s) 154, 322
SfcI CTRYAG 1 cut(s) 324
SmiMI CAYNNNNRTG 1 cut(s) 119
Sse9I AATT 2 cut(s) 225, 229
SsiI CCGC 3 cut(s) 55, 59, 83
TaiI ACGT 2 cut(s) 33, 48
TaqI TCGA 2 cut(s) 181, 239
TasI AATT 2 cut(s) 225, 229
TauI GCSGC 2 cut(s) 58, 61
TfiI GAWTC 1 cut(s) 132
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 1 cut(s) 96
TseFI GTSAC 1 cut(s) 254
TseI GCWGC 1 cut(s) 326
Tsp45I GTSAC 1 cut(s) 254
TspRI CASTG 1 cut(s) 96
VspI ATTAAT 1 cut(s) 228
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.