MD08G1080900.v1.1

NADH dehydrogenase ubiquinone iron-sulfur protein 4, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002607
Physical Location & Seq
Reverse (-)
6729707 .. 6732174
2468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1080900.v1.1.491

Sequence Viewer

Length: 483 bp
ATGGCCCGATCTCTACGACAGGGCTTCAACATCGCCCGAGCGCACGTGCTTTCGCGGCCACGATGGGCGTCGTCGCAGTCTTCGGAGGCGCTGGTGGAGGTCAATGTCCCCGACTTTGGCATGATCTCCGGAATTCCCGAACAGCATCTCCGACGAAGGGTTATAATCTACTCGCCTGCTCGAACTGCGAGCCAACAAGGTTCTGGGAAAGTCGGGAGATGGAAAATTAATTTTCTTTCGACGCAGAAGTGGGAAAACCCATTGATGGGATGGACATCTACAGGTGATCCCTACGCCAATGTTGGTGAAGCAGGACTGAGTTTTGAGAGTGAAGAAGCTGCGAAGGCATTTGCTGAGAAACATGGCTGGGATTATACGGTCAGGAAGCGTCACACTCCACTTTTGAAGGCTTATGCAGACAACTTCAAGTGGAAGGGCCCTGAAAAGAGTGAGAAGACTGCAGCTGATGCTGCAAACATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
ETC_C1_NDUFA4

Protein Analysis

161

Amino Acids

17.89

Weight (kDa)

9.81

Isoelectric Point (pI)

55.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUS4 PF04800 54 - 145 2.3e-29 NADH dehydrogenase ubiquinone Fe-S protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 164
AccII CGCG 1 cut(s) 55
AccIII TCCGGA 1 cut(s) 128
AciI CCGC 1 cut(s) 55
AclWI GGATC 1 cut(s) 281
AcoI YGGCCR 1 cut(s) 56
AcsI RAATTY 1 cut(s) 132
AcvI CACGTG 1 cut(s) 46
AcyI GRCGYC 1 cut(s) 68
AfiI CCNNNNNNNGG 4 cut(s) 116, 157, 265, 266
AgsI TTSAA 3 cut(s) 28, 406, 427
AloI GAACNNNNNNTCC 2 cut(s) 132, 164
AluBI AGCT 2 cut(s) 338, 464
AluI AGCT 2 cut(s) 338, 464
AlwI GGATC 1 cut(s) 281
Ama87I CYCGRG 1 cut(s) 36
Aor13HI TCCGGA 1 cut(s) 128
AoxI GGCC 3 cut(s) 3, 56, 436
ApaI GGGCCC 1 cut(s) 440
ApeKI GCWGC 3 cut(s) 338, 461, 470
ApoI RAATTY 1 cut(s) 132
AseI ATTAAT 1 cut(s) 228
AspLEI GCGC 2 cut(s) 43, 91
AspS9I GGNCC 3 cut(s) 4, 436, 437
AsuHPI GGTGA 2 cut(s) 296, 317
AvaI CYCGRG 1 cut(s) 36
BaeGI GKGCMC 1 cut(s) 440
BanII GRGCYC 1 cut(s) 440
BbrPI CACGTG 1 cut(s) 46
BbsI GAAGAC 2 cut(s) 72, 461
BbvI GCAGC 3 cut(s) 325, 457, 473
BccI CCATC 4 cut(s) 57, 213, 259, 264
BfmI CTRYAG 2 cut(s) 279, 459
BfoI RGCGCY 1 cut(s) 92
BisI GCNGC 4 cut(s) 56, 339, 462, 471
BlsI GCNGC 4 cut(s) 57, 340, 463, 472
BmeT110I CYCGRG 1 cut(s) 36
BmgT120I GGNCC 3 cut(s) 4, 436, 437
BmiI GGNNCC 1 cut(s) 438
BmsI GCATC 2 cut(s) 154, 457
BpiI GAAGAC 2 cut(s) 72, 461
BsaAI YACGTR 1 cut(s) 46
BsaBI GATNNNNATC 1 cut(s) 274
BsaHI GRCGYC 1 cut(s) 68
BsaWI WCCGGW 1 cut(s) 128
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 4 cut(s) 116, 157, 265, 266
Bse8I GATNNNNATC 1 cut(s) 274
BseAI TCCGGA 1 cut(s) 128
BseGI GGATG 1 cut(s) 275
BseJI GATNNNNATC 1 cut(s) 274
BseLI CCNNNNNNNGG 4 cut(s) 116, 157, 265, 266
BseMII CTCAG 2 cut(s) 308, 345
BseSI GKGCMC 1 cut(s) 440
BseXI GCAGC 3 cut(s) 325, 457, 473
BseYI CCCAGC 1 cut(s) 366
Bsh1236I CGCG 1 cut(s) 55
BshFI GGCC 3 cut(s) 5, 58, 438
BsiHKCI CYCGRG 1 cut(s) 36
BsiSI CCGG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 4 cut(s) 116, 157, 265, 266
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 3 cut(s) 5, 58, 438
BsoBI CYCGRG 1 cut(s) 36
Bsp120I GGGCCC 1 cut(s) 436
Bsp1286I GDGCHC 1 cut(s) 440
Bsp13I TCCGGA 1 cut(s) 128
Bsp143I GATC 3 cut(s) 8, 123, 286
BspACI CCGC 1 cut(s) 55
BspANI GGCC 3 cut(s) 5, 58, 438
BspCNI CTCAG 2 cut(s) 309, 346
BspEI TCCGGA 1 cut(s) 128
BspFNI CGCG 1 cut(s) 55
BspLI GGNNCC 1 cut(s) 438
BspMAI CTGCAG 1 cut(s) 463
BspPI GGATC 1 cut(s) 281
BssMI GATC 3 cut(s) 8, 123, 286
BssNI GRCGYC 1 cut(s) 68
Bst4CI ACNGT 1 cut(s) 379
BstACI GRCGYC 1 cut(s) 68
BstAPI GCANNNNNTGC 1 cut(s) 467
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 2 cut(s) 177, 190
BstDEI CTNAG 2 cut(s) 317, 354
BstF5I GGATG 1 cut(s) 275
BstFNI CGCG 1 cut(s) 55
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 2 cut(s) 43, 91
BstKTI GATC 3 cut(s) 11, 126, 289
BstMBI GATC 3 cut(s) 8, 123, 286
BstMWI GCNNNNNNNGC 5 cut(s) 55, 185, 344, 467, 470
BstSFI CTRYAG 2 cut(s) 279, 459
BstSLI GKGCMC 1 cut(s) 440
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 3 cut(s) 325, 457, 473
BstV2I GAAGAC 2 cut(s) 72, 461
BsuRI GGCC 3 cut(s) 5, 58, 438
BtgZI GCGATG 1 cut(s) 16
BtsCI GGATG 1 cut(s) 275
Cac8I GCNNGC 2 cut(s) 177, 190
CfoI GCGC 2 cut(s) 43, 91
Cfr13I GGNCC 3 cut(s) 4, 436, 437
CseI GACGC 3 cut(s) 57, 250, 377
CviAII CATG 2 cut(s) 121, 362
CviJI RGCY 9 cut(s) 5, 24, 58, 192, 338, 366, 410, 438, 464
CviKI_1 RGCY 9 cut(s) 5, 24, 58, 192, 338, 366, 410, 438, 464
DdeI CTNAG 2 cut(s) 317, 354
DpnI GATC 3 cut(s) 10, 125, 288
DpnII GATC 3 cut(s) 8, 123, 286
EaeI YGGCCR 1 cut(s) 56
Eco24I GRGCYC 1 cut(s) 440
Eco72I CACGTG 1 cut(s) 46
Eco88I CYCGRG 1 cut(s) 36
EcoO109I RGGNCCY 2 cut(s) 436, 437
EcoRI GAATTC 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 440
FaeI CATG 2 cut(s) 124, 365
FaiI YATR 5 cut(s) 122, 164, 363, 375, 414
FaqI GGGAC 1 cut(s) 92
FatI CATG 2 cut(s) 120, 361
Fnu4HI GCNGC 4 cut(s) 56, 339, 462, 471
FokI GGATG 1 cut(s) 282
FriOI GRGCYC 1 cut(s) 440
Fsp4HI GCNGC 4 cut(s) 56, 339, 462, 471
GlaI GCGC 2 cut(s) 42, 90
GluI GCNGC 4 cut(s) 56, 339, 462, 471
GsaI CCCAGC 1 cut(s) 370
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 3 cut(s) 5, 58, 438
HapII CCGG 1 cut(s) 129
HgaI GACGC 3 cut(s) 57, 250, 377
HhaI GCGC 2 cut(s) 43, 91
Hin1I GRCGYC 1 cut(s) 68
Hin1II CATG 2 cut(s) 124, 365
Hin6I GCGC 2 cut(s) 41, 89
HinP1I GCGC 2 cut(s) 41, 89
HpaII CCGG 1 cut(s) 129
HphI GGTGA 2 cut(s) 296, 317
Hpy188I TCNGA 3 cut(s) 85, 152, 482
Hpy188III TCNNGA 4 cut(s) 129, 137, 214, 382
Hpy99I CGWCG 4 cut(s) 73, 76, 156, 244
HpyAV CCTTC 4 cut(s) 150, 337, 400, 427
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 3 cut(s) 416, 461, 473
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 185, 344, 467, 470
HpyF3I CTNAG 2 cut(s) 317, 354
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 1 cut(s) 68
Hsp92II CATG 2 cut(s) 124, 365
HspAI GCGC 2 cut(s) 41, 89
Kpn2I TCCGGA 1 cut(s) 128
Kzo9I GATC 3 cut(s) 8, 123, 286
Lsp1109I GCAGC 3 cut(s) 325, 457, 473
LweI GCATC 2 cut(s) 154, 457
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 389
MalI GATC 3 cut(s) 10, 125, 288
MboI GATC 3 cut(s) 8, 123, 286
MboII GAAGA 3 cut(s) 72, 344, 466
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 3 cut(s) 132, 225, 229
MmeI TCCRAC 1 cut(s) 175
MnlI CCTC 2 cut(s) 79, 91
MroI TCCGGA 1 cut(s) 128
MseI TTAA 1 cut(s) 228
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 1 cut(s) 129
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 5 cut(s) 55, 185, 344, 467, 470
NdeII GATC 3 cut(s) 8, 123, 286
NlaIII CATG 2 cut(s) 124, 365
NlaIV GGNNCC 1 cut(s) 438
NmuCI GTSAC 1 cut(s) 389
PkrI GCNGC 4 cut(s) 57, 340, 463, 472
PmaCI CACGTG 1 cut(s) 46
PmlI CACGTG 1 cut(s) 46
Ppu21I YACGTR 1 cut(s) 46
PshBI ATTAAT 1 cut(s) 228
PsiI TTATAA 1 cut(s) 164
PspCI CACGTG 1 cut(s) 46
PspFI CCCAGC 1 cut(s) 366
PspN4I GGNNCC 1 cut(s) 438
PspOMI GGGCCC 1 cut(s) 436
PspPI GGNCC 3 cut(s) 4, 436, 437
PstI CTGCAG 1 cut(s) 463
PvuII CAGCTG 1 cut(s) 464
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 4 cut(s) 56, 339, 462, 471
Sau3AI GATC 3 cut(s) 8, 123, 286
Sau96I GGNCC 3 cut(s) 4, 436, 437
SduI GDGCHC 1 cut(s) 440
SetI ASST 6 cut(s) 48, 102, 202, 286, 340, 466
SfaNI GCATC 2 cut(s) 154, 457
SfcI CTRYAG 2 cut(s) 279, 459
Sse9I AATT 3 cut(s) 132, 225, 229
SsiI CCGC 1 cut(s) 55
TaaI ACNGT 1 cut(s) 379
TaiI ACGT 1 cut(s) 48
TaqI TCGA 2 cut(s) 181, 239
TasI AATT 3 cut(s) 132, 225, 229
TauI GCSGC 1 cut(s) 58
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 3 cut(s) 338, 461, 470
Tsp45I GTSAC 1 cut(s) 389
VspI ATTAAT 1 cut(s) 228
XapI RAATTY 1 cut(s) 132
XcmI CCANNNNNNNNNTGG 2 cut(s) 200, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.