Rh6DG488500

NADH dehydrogenase ubiquinone iron-sulfur protein 4, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002607
Physical Location & Seq
Forward (+)
64924826 .. 64928948
4123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG488500.1

Sequence Viewer

Length: 462 bp
ATGGCAAGCTCTCTACGACACGGATTCACCAAAGCCCGATCACCTATTCTCTCCCGGACCAGATGGTTCTCGTCGGAATCTCAGGCCCTGGTGGAGACCCGGATCCCCGACGTCGGAATCATATCGGGAATCCCCGAACAGCATCTCAAAAGAAGGGTCGTAATTTATTCTCCTGCTCGAACTGCGTCACAGCAAGGTTCTGGGAAAGTTGGGAAATGGAAAATTAATTTCCTTTCTACGCAGAAGTGGGAAAATCCATTGATGGGTTGGACATCCACAGGTGATCCCTATGCCAATGTGGGTGAGGCAGGACTGACTTTCGATAGTGAAGAAGCTGCAAGAGAATTTGCTGAGAAACATGGTTGGGATTATACGGTCAGGAAGCGTCACACACCACTTCTAAGGGTCAAGAGTTATGCAGACAACTTCAAGTGGAAGGGGCTACCAAAGAGCGAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04714 ko04723 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04714 map04723 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
ETC_C1_NDUFA4

Protein Analysis

153

Amino Acids

17.35

Weight (kDa)

9.88

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NDUS4 PF04800 52 - 146 1e-31 NADH dehydrogenase ubiquinone Fe-S protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 114
AclWI GGATC 3 cut(s) 97, 110, 278
AcsI RAATTY 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 111
AfiI CCNNNNNNNGG 2 cut(s) 113, 263
AgsI TTSAA 1 cut(s) 430
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 2 cut(s) 9, 335
AluI AGCT 2 cut(s) 9, 335
Alw26I GTCTC 1 cut(s) 89
AlwI GGATC 3 cut(s) 97, 110, 278
AlwNI CAGNNNCTG 1 cut(s) 88
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 1 cut(s) 335
ApoI RAATTY 1 cut(s) 344
ArsI GACNNNNNNTTYG 2 cut(s) 141, 173
AseI ATTAAT 1 cut(s) 225
AspS9I GGNCC 2 cut(s) 57, 85
AsuC2I CCSGG 2 cut(s) 55, 100
AsuHPI GGTGA 4 cut(s) 19, 33, 293, 314
AvaII GGWCC 1 cut(s) 57
BamHI GGATCC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 322
BccI CCATC 2 cut(s) 57, 256
BciT130I CCWGG 1 cut(s) 89
BcnI CCSGG 2 cut(s) 55, 100
BcoDI GTCTC 1 cut(s) 89
BisI GCNGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 337
Bme1390I CCNGG 3 cut(s) 55, 89, 100
Bme18I GGWCC 1 cut(s) 57
BmgT120I GGNCC 2 cut(s) 57, 85
BmiI GGNNCC 1 cut(s) 104
BmrFI CCNGG 3 cut(s) 55, 89, 100
BmsI GCATC 1 cut(s) 151
BpuMI CCSGG 2 cut(s) 55, 100
BsaHI GRCGYC 1 cut(s) 111
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 1 cut(s) 87
Bsc4I CCNNNNNNNGG 2 cut(s) 113, 263
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 1 cut(s) 272
BseLI CCNNNNNNNGG 2 cut(s) 113, 263
BseMII CTCAG 2 cut(s) 95, 342
BseXI GCAGC 1 cut(s) 322
BshFI GGCC 1 cut(s) 86
BsiSI CCGG 2 cut(s) 55, 100
BslI CCNNNNNNNGG 2 cut(s) 113, 263
BsmAI GTCTC 1 cut(s) 89
BsnI GGCC 1 cut(s) 86
Bso31I GGTCTC 1 cut(s) 89
Bsp143I GATC 3 cut(s) 38, 102, 283
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 2 cut(s) 94, 343
BspLI GGNNCC 1 cut(s) 104
BspPI GGATC 3 cut(s) 97, 110, 278
BspTNI GGTCTC 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 3 cut(s) 38, 102, 283
BssNI GRCGYC 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 376
BstACI GRCGYC 1 cut(s) 111
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 3 cut(s) 81, 351, 401
BstF5I GGATG 1 cut(s) 272
BstKTI GATC 3 cut(s) 41, 105, 286
BstMAI GTCTC 1 cut(s) 89
BstMBI GATC 3 cut(s) 38, 102, 283
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 1 cut(s) 89
BstSCI CCNGG 3 cut(s) 53, 87, 98
BstV1I GCAGC 1 cut(s) 322
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BsuRI GGCC 1 cut(s) 86
BtsCI GGATG 1 cut(s) 272
Cac8I GCNNGC 1 cut(s) 7
CaiI CAGNNNCTG 1 cut(s) 88
Cfr13I GGNCC 2 cut(s) 57, 85
CseI GACGC 2 cut(s) 174, 374
CviAII CATG 1 cut(s) 359
CviJI RGCY 5 cut(s) 9, 35, 86, 335, 442
CviKI_1 RGCY 5 cut(s) 9, 35, 86, 335, 442
DdeI CTNAG 3 cut(s) 81, 351, 401
DpnI GATC 3 cut(s) 40, 104, 285
DpnII GATC 3 cut(s) 38, 102, 283
Eco31I GGTCTC 1 cut(s) 89
Eco47I GGWCC 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 87
FaeI CATG 1 cut(s) 362
FaiI YATR 5 cut(s) 122, 291, 360, 372, 417
FatI CATG 1 cut(s) 358
Fnu4HI GCNGC 1 cut(s) 336
FokI GGATG 1 cut(s) 259
Fsp4HI GCNGC 1 cut(s) 336
GluI GCNGC 1 cut(s) 336
HaeIII GGCC 1 cut(s) 86
HapII CCGG 2 cut(s) 55, 100
HgaI GACGC 2 cut(s) 174, 374
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 1 cut(s) 362
HinfI GANTC 4 cut(s) 24, 77, 117, 129
HpaII CCGG 2 cut(s) 55, 100
HphI GGTGA 4 cut(s) 19, 33, 293, 314
Hpy188I TCNGA 2 cut(s) 76, 116
Hpy188III TCNNGA 3 cut(s) 126, 379, 409
Hpy99I CGWCG 3 cut(s) 76, 113, 116
HpyAV CCTTC 2 cut(s) 147, 430
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 2 cut(s) 338, 419
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 3 cut(s) 81, 351, 401
HpySE526I ACGT 1 cut(s) 111
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 1 cut(s) 362
Kzo9I GATC 3 cut(s) 38, 102, 283
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 1 cut(s) 151
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 2 cut(s) 186, 386
MalI GATC 3 cut(s) 40, 104, 285
MboI GATC 3 cut(s) 38, 102, 283
MboII GAAGA 1 cut(s) 341
MflI RGATCY 1 cut(s) 102
MluCI AATT 4 cut(s) 162, 222, 226, 344
MmeI TCCRAC 3 cut(s) 54, 94, 248
MnlI CCTC 2 cut(s) 298, 448
MseI TTAA 1 cut(s) 225
MspI CCGG 2 cut(s) 55, 100
MspR9I CCNGG 3 cut(s) 55, 89, 100
MvaI CCWGG 1 cut(s) 89
MwoI GCNNNNNNNGC 1 cut(s) 182
NciI CCSGG 2 cut(s) 55, 100
NdeII GATC 3 cut(s) 38, 102, 283
NlaIII CATG 1 cut(s) 362
NlaIV GGNNCC 1 cut(s) 104
NmuCI GTSAC 2 cut(s) 186, 386
PfeI GAWTC 4 cut(s) 24, 77, 117, 129
PfoI TCCNGGA 1 cut(s) 53
PkrI GCNGC 1 cut(s) 337
PshBI ATTAAT 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 1 cut(s) 104
PspPI GGNCC 2 cut(s) 57, 85
PstNI CAGNNNCTG 1 cut(s) 88
PsuI RGATCY 1 cut(s) 102
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 1 cut(s) 336
Sau3AI GATC 3 cut(s) 38, 102, 283
Sau96I GGNCC 2 cut(s) 57, 85
ScrFI CCNGG 3 cut(s) 55, 89, 100
SetI ASST 6 cut(s) 11, 46, 114, 199, 283, 337
SfaNI GCATC 1 cut(s) 151
SinI GGWCC 1 cut(s) 57
Sse9I AATT 4 cut(s) 162, 222, 226, 344
StyD4I CCNGG 3 cut(s) 53, 87, 98
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 1 cut(s) 114
TaqI TCGA 2 cut(s) 178, 321
TasI AATT 4 cut(s) 162, 222, 226, 344
TfiI GAWTC 4 cut(s) 24, 77, 117, 129
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TseFI GTSAC 2 cut(s) 186, 386
TseI GCWGC 1 cut(s) 335
Tsp45I GTSAC 2 cut(s) 186, 386
TspGWI ACGGA 1 cut(s) 36
VpaK11BI GGWCC 1 cut(s) 57
VspI ATTAAT 1 cut(s) 225
XapI RAATTY 1 cut(s) 344
XcmI CCANNNNNNNNNTGG 1 cut(s) 264
ZraI GACGTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.