FvH4_2g34810

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003463
Physical Location & Seq
Forward (+)
25772897 .. 25775123
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34810.t1

Sequence Viewer

Length: 1236 bp
ATGGAGAAAGCAAGTGCAATCCTTTCCCACCCCCCTCCCAGTCATGGAAAGCTCATCACTGTTCTCAGCATTGACGGAGGTGGAATCAAAGGGATTATTCCCGGAACTCTGCTCAGCTTCCTAGAGTCCGAGCTCCAGAGGCTGGATGGTGAAAATGCAAGGTTGGCCGATTACTTTGATGTTATCACCGGGACTAGCACAGGTGGCCTTGTCACCGCCATGATTACTTCCCCAAATGAAAACAAACGTCCCCTGTTTGCGGCTAGCCAGATTGTCGATTTTTACCTTGAACACTGTCCTAAAATCTTCCCTCAAATCAAGACAATTTTTGGTGACGCCTTAGGTGACGTCTTAGAAAATGCAAAAGGTGTATACGCACCAAAATATGATGGCAAGTATTTACACAAGCTTATCCAGGAAAAATTAGGAAACAGACGATTGAACCAGACATTGACCAATGTTGTGATCCCAACCTATGATATTAAGTGCCTTCAACCAGCTATCTTCTCCAGCTATGAGTTGAAAAGGAACAAGAAAATAGAGCCTCCTCTACTCTCTGATATATGCATCGGAACTTCAGCAGCACCAACTTATCTTCCCTCGCATTGTTTCAAAACCTCAACAAGAGAATACCACCTCGTTGATGGCGGAGTGGCTGCTAATAACCCGGCTTTGACTGCTATAACTGAAGTGACAAAAGAAATCAAGAACGGAAATTCGGATTTCTCTCGGATTGGGCCAGCTGAGCAGGGAGATAGGTTCCTACTTGTATCATTGGGCACTGGTTCATCAGAAAAATCTCATTATGATGCCGATAAAGCAAAATGCTGGGGAATTTTGGGATGGTTGATGCAGCCCGACTTCAAATGTCCATTGTTGGATGTACTTGGTGAAGCCAGTAGTGACATGGTAGATTGCCACATTTCTACCATTTTCCAGGCTCTTTGGTCTGATGATAACTACCTTCGAATTCAGGATGACAAACTGGATTCCAAACTGAATTCTATGGATAATGCCACGAAAGAAAACTTGAGAGACCTTAAGAAAGCGGGAGAGGCGCTGTTAAAGAAACGAGCTACAAAGTTGAATTTAGATACCGGTATCCATGAGCCTGATGAACGTAATATTACTAATGAAGAGGCTCTCAGAAGGGTGGCGAAAAGGCTCTCCCAAGAAAGGAAGGAACGTGCAGTCAGATCATCAGGAGTACAAGCAAACCCAGCATCAGCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

412

Amino Acids

45.2

Weight (kDa)

6.77

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 22 - 228 1.9e-19 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 351
AccB7I CCANNNNNTGG 1 cut(s) 142
AccI GTMKAC 1 cut(s) 372
AciI CCGC 4 cut(s) 216, 260, 648, 1049
AclWI GGATC 1 cut(s) 460
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 5 cut(s) 715, 834, 969, 1000, 1087
AcuI CTGAAG 2 cut(s) 561, 708
AcyI GRCGYC 2 cut(s) 336, 348
AfaI GTAC 2 cut(s) 885, 1209
AfiI CCNNNNNNNGG 4 cut(s) 44, 142, 259, 1176
AflII CTTAAG 1 cut(s) 1040
AgeI ACCGGT 1 cut(s) 1097
AgsI TTSAA 7 cut(s) 290, 442, 494, 523, 613, 865, 1087
AjnI CCWGG 2 cut(s) 414, 936
AluBI AGCT 9 cut(s) 52, 117, 133, 409, 500, 513, 743, 1076, 1233
AluI AGCT 9 cut(s) 52, 117, 133, 409, 500, 513, 743, 1076, 1233
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 1 cut(s) 1029
AlwI GGATC 1 cut(s) 460
AlwNI CAGNNNCTG 1 cut(s) 142
AoxI GGCC 3 cut(s) 165, 205, 737
ApeKI GCWGC 3 cut(s) 581, 656, 853
ApoI RAATTY 5 cut(s) 715, 834, 969, 1000, 1087
AsiGI ACCGGT 1 cut(s) 1097
AspLEI GCGC 1 cut(s) 1060
AspS9I GGNCC 1 cut(s) 737
AsuC2I CCSGG 3 cut(s) 102, 190, 668
AsuHPI GGTGA 6 cut(s) 161, 178, 205, 344, 356, 902
AsuII TTCGAA 1 cut(s) 967
AsuNHI GCTAGC 1 cut(s) 263
AxyI CCTNAGG 1 cut(s) 340
BaeGI GKGCMC 1 cut(s) 782
BanII GRGCYC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 3 cut(s) 593, 643, 865
BccI CCATC 4 cut(s) 140, 383, 638, 837
BciT130I CCWGG 2 cut(s) 416, 938
BciVI GTATCC 1 cut(s) 1112
BcnI CCSGG 3 cut(s) 102, 190, 668
BcoDI GTCTC 1 cut(s) 1029
BfaI CTAG 3 cut(s) 122, 195, 264
BfoI RGCGCY 1 cut(s) 1061
BfrI CTTAAG 1 cut(s) 1040
BfuI GTATCC 1 cut(s) 1112
BisI GCNGC 4 cut(s) 261, 582, 657, 854
BlpI GCTNAGC 2 cut(s) 113, 744
BlsI GCNGC 4 cut(s) 262, 583, 658, 855
Bme1390I CCNGG 5 cut(s) 102, 190, 416, 668, 938
BmgT120I GGNCC 1 cut(s) 737
BmiI GGNNCC 1 cut(s) 761
BmrFI CCNGG 5 cut(s) 102, 190, 416, 668, 938
BmrI ACTGGG 1 cut(s) 33
BmsI GCATC 4 cut(s) 576, 799, 840, 1232
BmtI GCTAGC 1 cut(s) 267
BmuI ACTGGG 1 cut(s) 33
BpmI CTGGAG 2 cut(s) 119, 493
Bpu1102I GCTNAGC 2 cut(s) 113, 744
Bpu14I TTCGAA 1 cut(s) 967
BpuEI CTTGAG 1 cut(s) 1051
BpuMI CCSGG 3 cut(s) 102, 190, 668
BsaHI GRCGYC 2 cut(s) 336, 348
BsaI GGTCTC 1 cut(s) 1029
BsaWI WCCGGW 1 cut(s) 1097
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 142, 259, 1176
Bse118I RCCGGY 1 cut(s) 1097
Bse1I ACTGG 4 cut(s) 39, 787, 897, 990
Bse21I CCTNAGG 1 cut(s) 340
BseBI CCWGG 2 cut(s) 416, 938
BseGI GGATG 4 cut(s) 151, 848, 886, 982
BseLI CCNNNNNNNGG 4 cut(s) 44, 142, 259, 1176
BseMII CTCAG 4 cut(s) 79, 127, 735, 1159
BseNI ACTGG 4 cut(s) 39, 787, 897, 990
BseRI GAGGAG 1 cut(s) 537
BseSI GKGCMC 1 cut(s) 782
BseXI GCAGC 3 cut(s) 593, 643, 865
BseYI CCCAGC 2 cut(s) 828, 1219
BsgI GTGCAG 1 cut(s) 1209
BshFI GGCC 3 cut(s) 167, 207, 739
BshTI ACCGGT 1 cut(s) 1097
BsiHKAI GWGCWC 1 cut(s) 135
BsiSI CCGG 4 cut(s) 102, 189, 668, 1098
BslFI GGGAC 2 cut(s) 205, 234
BslI CCNNNNNNNGG 4 cut(s) 44, 142, 259, 1176
BsmAI GTCTC 1 cut(s) 1029
BsmFI GGGAC 2 cut(s) 205, 234
BsnI GGCC 3 cut(s) 167, 207, 739
Bso31I GGTCTC 1 cut(s) 1029
Bsp119I TTCGAA 1 cut(s) 967
Bsp1286I GDGCHC 2 cut(s) 135, 782
Bsp143I GATC 2 cut(s) 465, 1196
Bsp1720I GCTNAGC 2 cut(s) 113, 744
BspACI CCGC 4 cut(s) 216, 260, 648, 1049
BspANI GGCC 3 cut(s) 167, 207, 739
BspCNI CTCAG 4 cut(s) 78, 126, 736, 1158
BspLI GGNNCC 1 cut(s) 761
BspOI GCTAGC 1 cut(s) 267
BspPI GGATC 1 cut(s) 460
BspT104I TTCGAA 1 cut(s) 967
BspTI CTTAAG 1 cut(s) 1040
BspTNI GGTCTC 1 cut(s) 1029
BsrFI RCCGGY 1 cut(s) 1097
BsrI ACTGG 4 cut(s) 39, 787, 897, 990
BssAI RCCGGY 1 cut(s) 1097
BssMI GATC 2 cut(s) 465, 1196
BssNAI GTATAC 1 cut(s) 373
BssNI GRCGYC 2 cut(s) 336, 348
Bst1107I GTATAC 1 cut(s) 373
Bst2UI CCWGG 2 cut(s) 416, 938
Bst4CI ACNGT 2 cut(s) 61, 296
Bst6I CTCTTC 1 cut(s) 1131
BstACI GRCGYC 2 cut(s) 336, 348
BstAFI CTTAAG 1 cut(s) 1040
BstBI TTCGAA 1 cut(s) 967
BstC8I GCNNGC 3 cut(s) 265, 741, 1231
BstDEI CTNAG 6 cut(s) 65, 113, 340, 352, 744, 1145
BstF5I GGATG 4 cut(s) 151, 848, 886, 982
BstH2I RGCGCY 1 cut(s) 1061
BstHHI GCGC 1 cut(s) 1060
BstKTI GATC 2 cut(s) 468, 1199
BstMAI GTCTC 1 cut(s) 1029
BstMBI GATC 2 cut(s) 465, 1196
BstMWI GCNNNNNNNGC 8 cut(s) 139, 164, 204, 677, 745, 818, 1055, 1220
BstNI CCWGG 2 cut(s) 416, 938
BstSCI CCNGG 5 cut(s) 100, 188, 414, 666, 936
BstSLI GKGCMC 1 cut(s) 782
BstV1I GCAGC 3 cut(s) 593, 643, 865
BstZ17I GTATAC 1 cut(s) 373
Bsu36I CCTNAGG 1 cut(s) 340
BsuI GTATCC 1 cut(s) 1112
BsuRI GGCC 3 cut(s) 167, 207, 739
BtsCI GGATG 4 cut(s) 151, 848, 886, 982
BtsIMutI CAGTG 3 cut(s) 57, 292, 780
Cac8I GCNNGC 3 cut(s) 265, 741, 1231
CaiI CAGNNNCTG 1 cut(s) 142
CfoI GCGC 1 cut(s) 1060
Cfr10I RCCGGY 1 cut(s) 1097
Cfr13I GGNCC 1 cut(s) 737
CseI GACGC 1 cut(s) 344
Csp6I GTAC 2 cut(s) 884, 1208
CspAI ACCGGT 1 cut(s) 1097
CviAII CATG 4 cut(s) 44, 220, 907, 1106
CviQI GTAC 2 cut(s) 884, 1208
DdeI CTNAG 6 cut(s) 65, 113, 340, 352, 744, 1145
DpnI GATC 2 cut(s) 467, 1198
DpnII GATC 2 cut(s) 465, 1196
EaeI YGGCCR 1 cut(s) 165
Eam1104I CTCTTC 1 cut(s) 1131
EarI CTCTTC 1 cut(s) 1131
EciI GGCGGA 1 cut(s) 663
Ecl136II GAGCTC 1 cut(s) 133
Eco24I GRGCYC 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 1029
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 2 cut(s) 561, 708
Eco81I CCTNAGG 1 cut(s) 340
EcoICRI GAGCTC 1 cut(s) 133
EcoRI GAATTC 2 cut(s) 969, 1000
EcoRII CCWGG 2 cut(s) 414, 936
EcoT22I ATGCAT 1 cut(s) 569
EcoT38I GRGCYC 1 cut(s) 135
FaeI CATG 4 cut(s) 47, 223, 910, 1109
FaqI GGGAC 2 cut(s) 205, 234
FatI CATG 4 cut(s) 43, 219, 906, 1105
FauI CCCGC 1 cut(s) 1042
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 4 cut(s) 261, 582, 657, 854
FokI GGATG 4 cut(s) 158, 855, 893, 989
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 4 cut(s) 261, 582, 657, 854
FspBI CTAG 3 cut(s) 122, 195, 264
GlaI GCGC 1 cut(s) 1059
GluI GCNGC 4 cut(s) 261, 582, 657, 854
GsaI CCCAGC 2 cut(s) 832, 1223
GsuI CTGGAG 2 cut(s) 119, 493
HaeII RGCGCY 1 cut(s) 1061
HaeIII GGCC 3 cut(s) 167, 207, 739
HapII CCGG 4 cut(s) 102, 189, 668, 1098
HgaI GACGC 1 cut(s) 344
HhaI GCGC 1 cut(s) 1060
Hin1I GRCGYC 2 cut(s) 336, 348
Hin1II CATG 4 cut(s) 47, 223, 910, 1109
Hin6I GCGC 1 cut(s) 1058
HinP1I GCGC 1 cut(s) 1058
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 3 cut(s) 84, 125, 989
HpaII CCGG 4 cut(s) 102, 189, 668, 1098
HphI GGTGA 6 cut(s) 161, 178, 205, 344, 356, 902
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 9 cut(s) 130, 559, 572, 721, 732, 793, 952, 1148, 1196
Hpy188III TCNNGA 5 cut(s) 136, 319, 706, 974, 1203
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 4 cut(s) 500, 974, 1143, 1174
HpyCH4III ACNGT 2 cut(s) 61, 296
HpyCH4IV ACGT 4 cut(s) 247, 348, 1120, 1186
HpyCH4V TGCA 6 cut(s) 17, 158, 362, 567, 853, 1190
HpyF10VI GCNNNNNNNGC 8 cut(s) 139, 164, 204, 677, 745, 818, 1055, 1220
HpyF3I CTNAG 6 cut(s) 65, 113, 340, 352, 744, 1145
HpySE526I ACGT 4 cut(s) 247, 348, 1120, 1186
Hsp92I GRCGYC 2 cut(s) 336, 348
Hsp92II CATG 4 cut(s) 47, 223, 910, 1109
HspAI GCGC 1 cut(s) 1058
Kzo9I GATC 2 cut(s) 465, 1196
LmnI GCTCC 1 cut(s) 138
Lsp1109I GCAGC 3 cut(s) 593, 643, 865
LweI GCATC 4 cut(s) 576, 799, 840, 1232
MaeI CTAG 3 cut(s) 122, 195, 264
MaeII ACGT 4 cut(s) 247, 348, 1120, 1186
MaeIII GTNAC 5 cut(s) 211, 332, 344, 691, 902
MalI GATC 2 cut(s) 467, 1198
MboI GATC 2 cut(s) 465, 1196
MboII GAAGA 4 cut(s) 298, 496, 587, 1148
MhlI GDGCHC 2 cut(s) 135, 782
MluCI AATT 7 cut(s) 324, 422, 715, 834, 969, 1000, 1087
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 858
Mph1103I ATGCAT 1 cut(s) 569
MseI TTAA 3 cut(s) 483, 1041, 1064
MslI CAYNNNNRTG 2 cut(s) 218, 807
MspA1I CMGCKG 2 cut(s) 743, 1233
MspCI CTTAAG 1 cut(s) 1040
MspI CCGG 4 cut(s) 102, 189, 668, 1098
MspR9I CCNGG 5 cut(s) 102, 190, 416, 668, 938
MvaI CCWGG 2 cut(s) 416, 938
MwoI GCNNNNNNNGC 8 cut(s) 139, 164, 204, 677, 745, 818, 1055, 1220
NciI CCSGG 3 cut(s) 102, 190, 668
NdeII GATC 2 cut(s) 465, 1196
NheI GCTAGC 1 cut(s) 263
NlaIII CATG 4 cut(s) 47, 223, 910, 1109
NlaIV GGNNCC 1 cut(s) 761
NmuCI GTSAC 5 cut(s) 211, 332, 344, 691, 902
NsiI ATGCAT 1 cut(s) 569
NspV TTCGAA 1 cut(s) 967
PfeI GAWTC 2 cut(s) 84, 989
PflMI CCANNNNNTGG 1 cut(s) 142
PfoI TCCNGGA 2 cut(s) 100, 414
PinAI ACCGGT 1 cut(s) 1097
PkrI GCNGC 4 cut(s) 262, 583, 658, 855
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
Psp124BI GAGCTC 1 cut(s) 135
Psp6I CCWGG 2 cut(s) 414, 936
PspFI CCCAGC 2 cut(s) 828, 1219
PspGI CCWGG 2 cut(s) 414, 936
PspN4I GGNNCC 1 cut(s) 761
PspPI GGNCC 1 cut(s) 737
PstNI CAGNNNCTG 1 cut(s) 142
PvuII CAGCTG 2 cut(s) 743, 1233
RsaI GTAC 2 cut(s) 885, 1209
RsaNI GTAC 2 cut(s) 884, 1208
RseI CAYNNNNRTG 2 cut(s) 218, 807
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 3 cut(s) 483, 1041, 1064
SatI GCNGC 4 cut(s) 261, 582, 657, 854
Sau3AI GATC 2 cut(s) 465, 1196
Sau96I GGNCC 1 cut(s) 737
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 5 cut(s) 102, 190, 416, 668, 938
SduI GDGCHC 2 cut(s) 135, 782
SfaNI GCATC 4 cut(s) 576, 799, 840, 1232
SfuI TTCGAA 1 cut(s) 967
SmiMI CAYNNNNRTG 2 cut(s) 218, 807
SmlI CTYRAG 2 cut(s) 1030, 1040
SmoI CTYRAG 2 cut(s) 1030, 1040
Sse9I AATT 7 cut(s) 324, 422, 715, 834, 969, 1000, 1087
SsiI CCGC 4 cut(s) 216, 260, 648, 1049
SspI AATATT 1 cut(s) 1126
SspMI CTAG 3 cut(s) 122, 195, 264
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 5 cut(s) 100, 188, 414, 666, 936
TaaI ACNGT 2 cut(s) 61, 296
TaiI ACGT 4 cut(s) 250, 351, 1123, 1189
TaqI TCGA 2 cut(s) 276, 967
TasI AATT 7 cut(s) 324, 422, 715, 834, 969, 1000, 1087
TatI WGTACW 2 cut(s) 883, 1207
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 2 cut(s) 84, 989
Tru1I TTAA 3 cut(s) 483, 1041, 1064
Tru9I TTAA 3 cut(s) 483, 1041, 1064
TscAI CASTG 3 cut(s) 64, 299, 787
TseFI GTSAC 5 cut(s) 211, 332, 344, 691, 902
TseI GCWGC 3 cut(s) 581, 656, 853
Tsp45I GTSAC 5 cut(s) 211, 332, 344, 691, 902
TspDTI ATGAA 4 cut(s) 252, 777, 1131, 1149
TspGWI ACGGA 2 cut(s) 90, 726
TspRI CASTG 3 cut(s) 64, 299, 787
Van91I CCANNNNNTGG 1 cut(s) 142
Vha464I CTTAAG 1 cut(s) 1040
XapI RAATTY 5 cut(s) 715, 834, 969, 1000, 1087
XcmI CCANNNNNNNNNTGG 3 cut(s) 143, 641, 904
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 3 cut(s) 122, 195, 264
ZraI GACGTC 1 cut(s) 349
Zsp2I ATGCAT 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.