RchiOBHm_Chr6g0307331

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003463
Physical Location & Seq
Reverse (-)
65776257 .. 65777639
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27631

Sequence Viewer

Length: 639 bp
ATGGAGAAAGCAAGTGCATTCCTTTGCCATCCCCGGAACTCTGGTGGAATCAAAGGGATTATCCCCAGAACTCTGCTCAGCTTCCTAGAGTCCGAGCTCCAGAAGCTGGATGCTGAAGCTGCAAGGTTGGCAGATTATTTTGATGTTATTGTAGGGACTAGCACAGGTGGCCTTGTCACCGCCATGCTTACGGCCCCAGATCAAAACAACCGTCCCTTGTTTGCGGCTAACCAGATTGTCGATTTTTACCTTGAGCACTGCCCTAAAATATTCCCTCAAATCAATATTCCACTGTTTGGTGACGCCTTAGAAAATGCAAAGGGTGCATACGCACCAAAATATAATGGCAAGTATTTACACAAGCTTATCAAGGAAAAATTAGGAGACAGACGATTGAACCAGACATTGACCAATGTTGTAATCCCAACTTTTGATATAAGTTGCCTCCAACCAGCTATCTTCTCCACCTACGAGTTGAAAAGGAACAAGAAATTAGATCCGGCTCTACTGTCGGATATATGCATCGGAACTTCAGCAGCACCAACTTATCTTCCCTCGCATTATTTCAAAACCGCAACAAGAGAATTCCATCTCGTTGATGGCGGTGTGGCTGCTAATAACCCGATATTAACTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

212

Amino Acids

23.34

Weight (kDa)

8.29

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 15 - 210 1.7e-18 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 106, 296
AciI CCGC 4 cut(s) 180, 224, 573, 603
AclWI GGATC 1 cut(s) 491
AcsI RAATTY 1 cut(s) 584
AcuI CTGAAG 2 cut(s) 135, 516
AcyI GRCGYC 1 cut(s) 303
AfiI CCNNNNNNNGG 2 cut(s) 106, 296
AgsI TTSAA 3 cut(s) 397, 478, 568
AluBI AGCT 6 cut(s) 81, 97, 106, 119, 364, 455
AluI AGCT 6 cut(s) 81, 97, 106, 119, 364, 455
Alw21I GWGCWC 2 cut(s) 99, 258
Alw26I GTCTC 1 cut(s) 378
AlwI GGATC 1 cut(s) 491
AlwNI CAGNNNCTG 1 cut(s) 106
AoxI GGCC 2 cut(s) 169, 192
ApeKI GCWGC 3 cut(s) 119, 536, 611
ApoI RAATTY 1 cut(s) 584
ArsI GACNNNNNNTTYG 2 cut(s) 196, 228
AspS9I GGNCC 1 cut(s) 193
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 2 cut(s) 169, 311
BanII GRGCYC 1 cut(s) 99
Bbv12I GWGCWC 2 cut(s) 99, 258
BbvI GCAGC 3 cut(s) 106, 548, 598
BccI CCATC 3 cut(s) 36, 593, 597
BceAI ACGGC 1 cut(s) 207
BcnI CCSGG 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 378
BfaI CTAG 2 cut(s) 86, 159
BisI GCNGC 4 cut(s) 120, 225, 537, 612
BlpI GCTNAGC 1 cut(s) 77
BlsI GCNGC 4 cut(s) 121, 226, 538, 613
Bme1390I CCNGG 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 1 cut(s) 34
BmsI GCATC 2 cut(s) 100, 531
BpmI CTGGAG 1 cut(s) 83
Bpu1102I GCTNAGC 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 34
BsaHI GRCGYC 1 cut(s) 303
BsaJI CCNNGG 1 cut(s) 32
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 296
BseDI CCNNGG 1 cut(s) 32
BseGI GGATG 2 cut(s) 28, 115
BseLI CCNNNNNNNGG 2 cut(s) 106, 296
BseMII CTCAG 1 cut(s) 91
BseXI GCAGC 3 cut(s) 106, 548, 598
BshFI GGCC 2 cut(s) 171, 194
BsiHKAI GWGCWC 2 cut(s) 99, 258
BsiSI CCGG 2 cut(s) 34, 500
BslFI GGGAC 2 cut(s) 169, 198
BslI CCNNNNNNNGG 2 cut(s) 106, 296
BsmAI GTCTC 1 cut(s) 378
BsmFI GGGAC 2 cut(s) 169, 198
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 2 cut(s) 171, 194
Bsp1286I GDGCHC 2 cut(s) 99, 258
Bsp143I GATC 2 cut(s) 199, 496
Bsp1720I GCTNAGC 1 cut(s) 77
BspACI CCGC 4 cut(s) 180, 224, 573, 603
BspANI GGCC 2 cut(s) 171, 194
BspCNI CTCAG 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 195
BspPI GGATC 1 cut(s) 491
BssECI CCNNGG 1 cut(s) 32
BssMI GATC 2 cut(s) 199, 496
BssNI GRCGYC 1 cut(s) 303
Bst4CI ACNGT 3 cut(s) 212, 294, 510
BstACI GRCGYC 1 cut(s) 303
BstAPI GCANNNNNTGC 1 cut(s) 323
BstDEI CTNAG 2 cut(s) 77, 307
BstF5I GGATG 2 cut(s) 28, 115
BstKTI GATC 2 cut(s) 202, 499
BstMAI GTCTC 1 cut(s) 378
BstMBI GATC 2 cut(s) 199, 496
BstMWI GCNNNNNNNGC 5 cut(s) 103, 119, 128, 168, 323
BstSCI CCNGG 1 cut(s) 32
BstV1I GCAGC 3 cut(s) 106, 548, 598
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
BsuRI GGCC 2 cut(s) 171, 194
BtsCI GGATG 2 cut(s) 28, 115
BtsI GCAGTG 1 cut(s) 256
BtsIMutI CAGTG 2 cut(s) 256, 290
CaiI CAGNNNCTG 1 cut(s) 106
Cfr13I GGNCC 1 cut(s) 193
CseI GACGC 1 cut(s) 311
CviAII CATG 1 cut(s) 184
DdeI CTNAG 2 cut(s) 77, 307
DpnI GATC 2 cut(s) 201, 498
DpnII GATC 2 cut(s) 199, 496
Ecl136II GAGCTC 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 99
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 2 cut(s) 135, 516
EcoICRI GAGCTC 1 cut(s) 97
EcoRI GAATTC 1 cut(s) 584
EcoT22I ATGCAT 1 cut(s) 524
EcoT38I GRGCYC 1 cut(s) 99
FaeI CATG 1 cut(s) 187
FaiI YATR 6 cut(s) 185, 328, 342, 437, 518, 520
FaqI GGGAC 2 cut(s) 169, 198
FatI CATG 1 cut(s) 183
Fnu4HI GCNGC 4 cut(s) 120, 225, 537, 612
FokI GGATG 2 cut(s) 15, 122
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 4 cut(s) 120, 225, 537, 612
FspBI CTAG 2 cut(s) 86, 159
GluI GCNGC 4 cut(s) 120, 225, 537, 612
GsuI CTGGAG 1 cut(s) 83
HaeIII GGCC 2 cut(s) 171, 194
HapII CCGG 2 cut(s) 34, 500
HgaI GACGC 1 cut(s) 311
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 1 cut(s) 187
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 2 cut(s) 48, 89
HpaII CCGG 2 cut(s) 34, 500
HphI GGTGA 2 cut(s) 169, 311
Hpy188I TCNGA 3 cut(s) 94, 514, 527
Hpy188III TCNNGA 1 cut(s) 100
HpyCH4III ACNGT 3 cut(s) 212, 294, 510
HpyCH4V TGCA 5 cut(s) 17, 122, 317, 326, 522
HpyF10VI GCNNNNNNNGC 5 cut(s) 103, 119, 128, 168, 323
HpyF3I CTNAG 2 cut(s) 77, 307
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 1 cut(s) 187
Kzo9I GATC 2 cut(s) 199, 496
LmnI GCTCC 1 cut(s) 102
Lsp1109I GCAGC 3 cut(s) 106, 548, 598
LweI GCATC 2 cut(s) 100, 531
MaeI CTAG 2 cut(s) 86, 159
MaeIII GTNAC 2 cut(s) 175, 299
MalI GATC 2 cut(s) 201, 498
MboI GATC 2 cut(s) 199, 496
MboII GAAGA 2 cut(s) 451, 542
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 2 cut(s) 99, 258
MluCI AATT 4 cut(s) 377, 491, 584, 634
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 2 cut(s) 472, 492
MnlI CCTC 3 cut(s) 285, 455, 565
Mph1103I ATGCAT 1 cut(s) 524
MseI TTAA 1 cut(s) 629
MslI CAYNNNNRTG 1 cut(s) 182
MspI CCGG 2 cut(s) 34, 500
MspR9I CCNGG 1 cut(s) 34
Mva1269I GAATGC 1 cut(s) 17
MwoI GCNNNNNNNGC 5 cut(s) 103, 119, 128, 168, 323
NciI CCSGG 1 cut(s) 34
NdeII GATC 2 cut(s) 199, 496
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 1 cut(s) 195
NmuCI GTSAC 2 cut(s) 175, 299
NsiI ATGCAT 1 cut(s) 524
PctI GAATGC 1 cut(s) 17
PfeI GAWTC 1 cut(s) 48
PflMI CCANNNNNTGG 2 cut(s) 106, 296
PkrI GCNGC 4 cut(s) 121, 226, 538, 613
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
Psp124BI GAGCTC 1 cut(s) 99
PspN4I GGNNCC 1 cut(s) 195
PspPI GGNCC 1 cut(s) 193
PstNI CAGNNNCTG 1 cut(s) 106
PsuI RGATCY 1 cut(s) 496
RseI CAYNNNNRTG 1 cut(s) 182
SacI GAGCTC 1 cut(s) 99
SaqAI TTAA 1 cut(s) 629
SatI GCNGC 4 cut(s) 120, 225, 537, 612
Sau3AI GATC 2 cut(s) 199, 496
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 1 cut(s) 34
SduI GDGCHC 2 cut(s) 99, 258
SfaNI GCATC 2 cut(s) 100, 531
SmiMI CAYNNNNRTG 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 4 cut(s) 377, 491, 584, 634
SsiI CCGC 4 cut(s) 180, 224, 573, 603
SspI AATATT 2 cut(s) 270, 286
SspMI CTAG 2 cut(s) 86, 159
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 1 cut(s) 32
TaaI ACNGT 3 cut(s) 212, 294, 510
TaqI TCGA 1 cut(s) 240
TasI AATT 4 cut(s) 377, 491, 584, 634
TauI GCSGC 1 cut(s) 227
TfiI GAWTC 1 cut(s) 48
Tru1I TTAA 1 cut(s) 629
Tru9I TTAA 1 cut(s) 629
TscAI CASTG 2 cut(s) 263, 297
TseFI GTSAC 2 cut(s) 175, 299
TseI GCWGC 3 cut(s) 119, 536, 611
Tsp45I GTSAC 2 cut(s) 175, 299
TspRI CASTG 2 cut(s) 263, 297
Van91I CCANNNNNTGG 2 cut(s) 106, 296
XapI RAATTY 1 cut(s) 584
XcmI CCANNNNNNNNNTGG 1 cut(s) 596
XspI CTAG 2 cut(s) 86, 159
Zsp2I ATGCAT 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.