Rorug06G0358100

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003463
Physical Location & Seq
Forward (+)
50718824 .. 50719453
630 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0358100.1

Sequence Viewer

Length: 630 bp
ATGTATAGTCTAAGGAGAGCAAAGCTCTTGCTCTCATTGCTTCCAACTTCTGCCTTCATCCTCATCCTATTCTTTTCACAAAGCCACTTCACAAATAGCCCCAAGCTCTTGCACACCAAGAACAAGCTCACCACCGGTCAGCAGCGAGATCTCGACGACTGTCTCGAGCTCTTCGACAACACCATTTCTGAGCTCAAGGCTGTCATGTCGGAACTCAACTCCAAGAAGTCGCCGGCGGCCAAGCACTACAACCACTTGAGGACGCTGCTCAGCGCCGCAATGACGAACCAGTACACGTGTCTCGAAGGGTTTGATTCCAGCAGTAGTACGGCGAAAGCTGTGAAGGATGAATTGGAGAGCAGATTGAGTACTACTACCTGCAAGCAAGTTAGAAATGCCCTTGTGATGCTCAAGAAGATTCCGGGAGTGGTTGAACGTGAGACTGACGAGTACGGACCCATGAAACACGAGTTCCCTACATGGGTCGGATCCAAAGCTCGAAAACTGTTGCAGACTAGTTCGGCAAATCAGACCCAGTATCCTCTTGTGGTGGCGAAAGATGGTAGCGGAAACTTCACCACCATCATCGACGCAGTGGCTGCTGCTCCAAATTCGAGCGCAACCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

209

Amino Acids

23.13

Weight (kDa)

9.34

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 35 - 136 1.5e-18 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 386
AciI CCGC 3 cut(s) 236, 276, 567
AclWI GGATC 2 cut(s) 483, 496
AcoI YGGCCR 1 cut(s) 237
AcsI RAATTY 1 cut(s) 610
AcvI CACGTG 1 cut(s) 297
AfaI GTAC 4 cut(s) 293, 328, 370, 452
AfiI CCNNNNNNNGG 1 cut(s) 481
AflIII ACRYGT 2 cut(s) 294, 296
AgeI ACCGGT 1 cut(s) 134
AgsI TTSAA 1 cut(s) 434
AhlI ACTAGT 1 cut(s) 515
AjnI CCWGG 1 cut(s) 623
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
AluBI AGCT 7 cut(s) 25, 106, 127, 169, 193, 338, 497
AluI AGCT 7 cut(s) 25, 106, 127, 169, 193, 338, 497
Alw21I GWGCWC 2 cut(s) 171, 195
Alw26I GTCTC 3 cut(s) 167, 305, 434
AlwI GGATC 2 cut(s) 483, 496
AlwNI CAGNNNCTG 1 cut(s) 599
Ama87I CYCGRG 1 cut(s) 164
AoxI GGCC 1 cut(s) 237
ApeKI GCWGC 4 cut(s) 142, 265, 599, 602
ApoI RAATTY 1 cut(s) 610
AsiGI ACCGGT 1 cut(s) 134
AspLEI GCGC 2 cut(s) 275, 620
AspS9I GGNCC 1 cut(s) 455
AsuC2I CCSGG 1 cut(s) 423
AsuHPI GGTGA 2 cut(s) 121, 568
AvaI CYCGRG 1 cut(s) 164
AvaII GGWCC 1 cut(s) 455
BamHI GGATCC 1 cut(s) 488
BanII GRGCYC 2 cut(s) 171, 195
BauI CACGAG 1 cut(s) 467
BbrPI CACGTG 1 cut(s) 297
Bbv12I GWGCWC 2 cut(s) 171, 195
BbvI GCAGC 4 cut(s) 154, 252, 586, 589
BccI CCATC 2 cut(s) 554, 590
BceAI ACGGC 1 cut(s) 345
BciT130I CCWGG 1 cut(s) 625
BciVI GTATCC 1 cut(s) 549
BcnI CCSGG 1 cut(s) 423
BcoDI GTCTC 3 cut(s) 167, 305, 434
BcuI ACTAGT 1 cut(s) 515
BfaI CTAG 1 cut(s) 516
BfoI RGCGCY 1 cut(s) 276
BfuAI ACCTGC 1 cut(s) 386
BfuI GTATCC 1 cut(s) 549
BglII AGATCT 1 cut(s) 148
BisI GCNGC 6 cut(s) 143, 237, 266, 276, 600, 603
BlpI GCTNAGC 1 cut(s) 269
BlsI GCNGC 6 cut(s) 144, 238, 267, 277, 601, 604
BmcAI AGTACT 1 cut(s) 370
Bme1390I CCNGG 2 cut(s) 423, 625
Bme18I GGWCC 1 cut(s) 455
BmeT110I CYCGRG 1 cut(s) 164
BmgT120I GGNCC 1 cut(s) 455
BmiI GGNNCC 2 cut(s) 457, 490
BmrFI CCNGG 2 cut(s) 423, 625
BmrI ACTGGG 1 cut(s) 529
BmsI GCATC 1 cut(s) 396
BmuI ACTGGG 1 cut(s) 529
BoxI GACNNNNGTC 1 cut(s) 159
BplI GAGNNNNNCTC 2 cut(s) 9, 41
Bpu1102I GCTNAGC 1 cut(s) 269
BpuEI CTTGAG 3 cut(s) 179, 277, 395
BpuMI CCSGG 1 cut(s) 423
BsaAI YACGTR 1 cut(s) 297
BsaWI WCCGGW 1 cut(s) 134
Bsc4I CCNNNNNNNGG 1 cut(s) 481
Bse118I RCCGGY 2 cut(s) 134, 232
Bse1I ACTGG 2 cut(s) 289, 535
Bse3DI GCAATG 2 cut(s) 35, 285
BseBI CCWGG 1 cut(s) 625
BseGI GGATG 3 cut(s) 57, 63, 352
BseLI CCNNNNNNNGG 1 cut(s) 481
BseMI GCAATG 2 cut(s) 35, 285
BseMII CTCAG 2 cut(s) 180, 283
BseNI ACTGG 2 cut(s) 289, 535
BseXI GCAGC 4 cut(s) 154, 252, 586, 589
BshFI GGCC 1 cut(s) 239
BshTI ACCGGT 1 cut(s) 134
BsiHKAI GWGCWC 2 cut(s) 171, 195
BsiHKCI CYCGRG 1 cut(s) 164
BsiSI CCGG 3 cut(s) 135, 233, 422
BslI CCNNNNNNNGG 1 cut(s) 481
BsmAI GTCTC 3 cut(s) 167, 305, 434
BsnI GGCC 1 cut(s) 239
BsoBI CYCGRG 1 cut(s) 164
Bsp1286I GDGCHC 2 cut(s) 171, 195
Bsp143I GATC 2 cut(s) 148, 488
Bsp1720I GCTNAGC 1 cut(s) 269
BspACI CCGC 3 cut(s) 236, 276, 567
BspANI GGCC 1 cut(s) 239
BspCNI CTCAG 2 cut(s) 181, 282
BspLI GGNNCC 2 cut(s) 457, 490
BspMI ACCTGC 1 cut(s) 386
BspPI GGATC 2 cut(s) 483, 496
BspQI GCTCTTC 1 cut(s) 176
BsrDI GCAATG 2 cut(s) 35, 285
BsrFI RCCGGY 2 cut(s) 134, 232
BsrI ACTGG 2 cut(s) 289, 535
BssAI RCCGGY 2 cut(s) 134, 232
BssMI GATC 2 cut(s) 148, 488
BssSI CACGAG 1 cut(s) 467
Bst2BI CACGAG 1 cut(s) 467
Bst2UI CCWGG 1 cut(s) 625
Bst4CI ACNGT 2 cut(s) 161, 507
Bst6I CTCTTC 1 cut(s) 176
BstAPI GCANNNNNTGC 1 cut(s) 599
BstBAI YACGTR 1 cut(s) 297
BstC8I GCNNGC 2 cut(s) 234, 383
BstDEI CTNAG 3 cut(s) 11, 189, 269
BstF5I GGATG 3 cut(s) 57, 63, 352
BstH2I RGCGCY 1 cut(s) 276
BstHHI GCGC 2 cut(s) 275, 620
BstKTI GATC 2 cut(s) 151, 491
BstMAI GTCTC 3 cut(s) 167, 305, 434
BstMBI GATC 2 cut(s) 148, 488
BstMWI GCNNNNNNNGC 2 cut(s) 37, 599
BstNI CCWGG 1 cut(s) 625
BstPAI GACNNNNGTC 1 cut(s) 159
BstSCI CCNGG 2 cut(s) 421, 623
BstV1I GCAGC 4 cut(s) 154, 252, 586, 589
BstX2I RGATCY 2 cut(s) 148, 488
BstYI RGATCY 2 cut(s) 148, 488
BsuI GTATCC 1 cut(s) 549
BsuRI GGCC 1 cut(s) 239
BtsCI GGATG 3 cut(s) 57, 63, 352
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 1 cut(s) 600
BveI ACCTGC 1 cut(s) 386
Cac8I GCNNGC 2 cut(s) 234, 383
CaiI CAGNNNCTG 1 cut(s) 599
CfoI GCGC 2 cut(s) 275, 620
Cfr10I RCCGGY 2 cut(s) 134, 232
Cfr13I GGNCC 1 cut(s) 455
CseI GACGC 2 cut(s) 271, 599
CsiI ACCWGGT 1 cut(s) 623
Csp6I GTAC 4 cut(s) 292, 327, 369, 451
CspAI ACCGGT 1 cut(s) 134
CviAII CATG 3 cut(s) 205, 460, 480
CviQI GTAC 4 cut(s) 292, 327, 369, 451
DdeI CTNAG 3 cut(s) 11, 189, 269
DpnI GATC 2 cut(s) 150, 490
DpnII GATC 2 cut(s) 148, 488
EaeI YGGCCR 1 cut(s) 237
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
Ecl136II GAGCTC 2 cut(s) 169, 193
Eco24I GRGCYC 2 cut(s) 171, 195
Eco47I GGWCC 1 cut(s) 455
Eco53kI GAGCTC 2 cut(s) 169, 193
Eco72I CACGTG 1 cut(s) 297
Eco88I CYCGRG 1 cut(s) 164
EcoICRI GAGCTC 2 cut(s) 169, 193
EcoRII CCWGG 1 cut(s) 623
EcoT38I GRGCYC 2 cut(s) 171, 195
FaeI CATG 3 cut(s) 208, 463, 483
FaiI YATR 4 cut(s) 6, 206, 461, 481
FatI CATG 3 cut(s) 204, 459, 479
Fnu4HI GCNGC 6 cut(s) 143, 237, 266, 276, 600, 603
FokI GGATG 3 cut(s) 44, 50, 359
FriOI GRGCYC 2 cut(s) 171, 195
Fsp4HI GCNGC 6 cut(s) 143, 237, 266, 276, 600, 603
FspBI CTAG 1 cut(s) 516
GlaI GCGC 2 cut(s) 274, 619
GluI GCNGC 6 cut(s) 143, 237, 266, 276, 600, 603
HaeII RGCGCY 1 cut(s) 276
HaeIII GGCC 1 cut(s) 239
HapII CCGG 3 cut(s) 135, 233, 422
HgaI GACGC 2 cut(s) 271, 599
HhaI GCGC 2 cut(s) 275, 620
Hin1II CATG 3 cut(s) 208, 463, 483
Hin6I GCGC 2 cut(s) 273, 618
HinP1I GCGC 2 cut(s) 273, 618
HinfI GANTC 2 cut(s) 314, 418
HpaII CCGG 3 cut(s) 135, 233, 422
HphI GGTGA 2 cut(s) 121, 568
Hpy166II GTNNAC 1 cut(s) 294
Hpy188I TCNGA 4 cut(s) 190, 211, 488, 531
Hpy188III TCNNGA 4 cut(s) 152, 164, 302, 412
Hpy8I GTNNAC 1 cut(s) 294
Hpy99I CGWCG 2 cut(s) 158, 593
HpyAV CCTTC 3 cut(s) 64, 299, 337
HpyCH4III ACNGT 2 cut(s) 161, 507
HpyCH4IV ACGT 2 cut(s) 296, 436
HpyCH4V TGCA 3 cut(s) 112, 381, 511
HpyF10VI GCNNNNNNNGC 2 cut(s) 37, 599
HpyF3I CTNAG 3 cut(s) 11, 189, 269
HpySE526I ACGT 2 cut(s) 296, 436
Hsp92II CATG 3 cut(s) 208, 463, 483
HspAI GCGC 2 cut(s) 273, 618
KroI GCCGGC 1 cut(s) 232
KroNI GCCGGC 1 cut(s) 234
Kzo9I GATC 2 cut(s) 148, 488
LguI GCTCTTC 1 cut(s) 176
LmnI GCTCC 1 cut(s) 610
LpnPI CCDG 8 cut(s) 148, 246, 302, 331, 391, 435, 548, 610
Lsp1109I GCAGC 4 cut(s) 154, 252, 586, 589
LweI GCATC 1 cut(s) 396
MabI ACCWGGT 1 cut(s) 623
MaeI CTAG 1 cut(s) 516
MaeII ACGT 2 cut(s) 296, 436
MalI GATC 2 cut(s) 150, 490
MboI GATC 2 cut(s) 148, 488
MboII GAAGA 2 cut(s) 163, 427
MflI RGATCY 2 cut(s) 148, 488
MhlI GDGCHC 2 cut(s) 171, 195
MluCI AATT 2 cut(s) 350, 610
MmeI TCCRAC 3 cut(s) 68, 189, 466
MnlI CCTC 3 cut(s) 71, 252, 552
MreI CGCCGGCG 1 cut(s) 232
MroNI GCCGGC 1 cut(s) 232
MspI CCGG 3 cut(s) 135, 233, 422
MspR9I CCNGG 2 cut(s) 423, 625
MvaI CCWGG 1 cut(s) 625
MwoI GCNNNNNNNGC 2 cut(s) 37, 599
NaeI GCCGGC 1 cut(s) 234
NciI CCSGG 1 cut(s) 423
NdeII GATC 2 cut(s) 148, 488
NgoMIV GCCGGC 1 cut(s) 232
NlaIII CATG 3 cut(s) 208, 463, 483
NlaIV GGNNCC 2 cut(s) 457, 490
PaeR7I CTCGAG 1 cut(s) 164
PciSI GCTCTTC 1 cut(s) 176
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 171
PdiI GCCGGC 1 cut(s) 234
PfeI GAWTC 2 cut(s) 314, 418
PfoI TCCNGGA 1 cut(s) 421
PinAI ACCGGT 1 cut(s) 134
PkrI GCNGC 6 cut(s) 144, 238, 267, 277, 601, 604
PmaCI CACGTG 1 cut(s) 297
PmlI CACGTG 1 cut(s) 297
Ppu21I YACGTR 1 cut(s) 297
PshAI GACNNNNGTC 1 cut(s) 159
Psp124BI GAGCTC 2 cut(s) 171, 195
Psp6I CCWGG 1 cut(s) 623
PspCI CACGTG 1 cut(s) 297
PspGI CCWGG 1 cut(s) 623
PspN4I GGNNCC 2 cut(s) 457, 490
PspPI GGNCC 1 cut(s) 455
PstNI CAGNNNCTG 1 cut(s) 599
PsuI RGATCY 2 cut(s) 148, 488
RsaI GTAC 4 cut(s) 293, 328, 370, 452
RsaNI GTAC 4 cut(s) 292, 327, 369, 451
SacI GAGCTC 2 cut(s) 171, 195
SapI GCTCTTC 1 cut(s) 176
SatI GCNGC 6 cut(s) 143, 237, 266, 276, 600, 603
Sau3AI GATC 2 cut(s) 148, 488
Sau96I GGNCC 1 cut(s) 455
ScaI AGTACT 1 cut(s) 370
ScrFI CCNGG 2 cut(s) 423, 625
SduI GDGCHC 2 cut(s) 171, 195
SexAI ACCWGGT 1 cut(s) 623
SfaNI GCATC 1 cut(s) 396
Sfr274I CTCGAG 1 cut(s) 164
SgrAI CRCCGGYG 1 cut(s) 232
SinI GGWCC 1 cut(s) 455
SlaI CTCGAG 1 cut(s) 164
SmlI CTYRAG 4 cut(s) 164, 194, 256, 410
SmoI CTYRAG 4 cut(s) 164, 194, 256, 410
SpeI ACTAGT 1 cut(s) 515
Sse9I AATT 2 cut(s) 350, 610
SsiI CCGC 3 cut(s) 236, 276, 567
SspMI CTAG 1 cut(s) 516
SstI GAGCTC 2 cut(s) 171, 195
StyD4I CCNGG 2 cut(s) 421, 623
TaaI ACNGT 2 cut(s) 161, 507
TaiI ACGT 2 cut(s) 299, 439
TaqI TCGA 7 cut(s) 153, 165, 174, 303, 499, 588, 614
TasI AATT 2 cut(s) 350, 610
TatI WGTACW 2 cut(s) 291, 368
TauI GCSGC 2 cut(s) 239, 278
TfiI GAWTC 2 cut(s) 314, 418
TscAI CASTG 1 cut(s) 600
TseI GCWGC 4 cut(s) 142, 265, 599, 602
TspDTI ATGAA 3 cut(s) 46, 363, 476
TspGWI ACGGA 1 cut(s) 468
TspRI CASTG 1 cut(s) 600
VpaK11BI GGWCC 1 cut(s) 455
XapI RAATTY 1 cut(s) 610
XhoI CTCGAG 1 cut(s) 164
XspI CTAG 1 cut(s) 516
ZrmI AGTACT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.