RchiOBHm_Chr6g0307671

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003463
Physical Location & Seq
Reverse (-)
65931472 .. 65933469
1998 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27661

Sequence Viewer

Length: 1137 bp
ATGGAGAAAGCAGGTGCATTCCTTTGCCATCCCCTCCCTCACATGGAAAGCTCATCACTGTTCTCAGCATTGATGGAGGTGGAATCAAAGGGATTATCCCGGGACTCTACTCAGCTTCCTAGAAAGCTGGATGGTGAGGCTGCAAGGTTGGCAGATTATTTCGATGTTATTGCAGGGACTAGCACAGGTGGCCTTGTCACTGCCATGCTTGCCGCCCCGGATCAAAACAACCGTCCCTTGTTTGCGGCTAACCAGATTGTCGATTTTTACCTTGAGCACTGCCCTAAAATATTCCCTCAAATCAATATTCCACTGTTTGGTGACGCCTTAGAAAATGCAAAGGGTGTATACGCTCCAAAATATGATGGCAAGTATTTACACAAACTTATCAAGGAAAAATTAGGAGATAGACGATTGAACCAGACATTGACCAATGTTGTGATCCCAACTTTTGATATAAGTTGCCTCCAACCAGCTATCTTCTCCACTACGAGGAACAAGAAATTAGATCCGGCTCTACTGTCGGATATATGCATCGGAACTTCAGCAGCACCAACTTATCTTCCCTCGCATTATTTCAAAACCGCAACAAGAGAATTCCATCTCGTTGATGGCGGTGTGGCTGCTAATAACCCGGTTCCTCCTCATATCTTGGGTACTGGTTCATCAGCAAAATCCAAGTACGATGCCAGAGTAGCAGCTAAGTGGGGTATTATGGCATGGTTGATGCAAGCCAACTTTCAATGTCCATTGTTAGATGTACTCACTGAAGCAAGTAGTGACATGGTAGATTGCCACCTTTCTACCCTTTTCCAAGCTCTTTGGTCTGACGATAACTACCTTCGAATTCAGGATGACCAATTGAGCACCGACTTGAATTCTATGGATAATGCTACGCCCGAAAACTTGAAAAAACTTGTAAAAGTGGGAGAGGCACTGTTAAAGAAACAAGCTTCAAAGTTGAATATAGTTACCGGGCTCCATGAGCCTGATGAACGTCATATTACTAATGAAGAGGCTCTCAGAAGGTACTTATTGATTACAATTACTCTCTGTACGTACTGGGATTTGTATCAAAGCATCATAAACATATATTCATTACTATTATTATTATTAATTAGTTATTTGTTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

378

Amino Acids

42.01

Weight (kDa)

5.94

Isoelectric Point (pI)

38.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 49 - 214 2.1e-17 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2
Acc36I ACCTGC 1 cut(s) 2
AccB7I CCANNNNNTGG 1 cut(s) 317
AccI GTMKAC 1 cut(s) 348
AciI CCGC 4 cut(s) 213, 245, 585, 615
AclWI GGATC 3 cut(s) 228, 436, 503
AcsI RAATTY 3 cut(s) 596, 846, 877
AcuI CTGAAG 2 cut(s) 528, 789
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 6 cut(s) 658, 683, 762, 1031, 1057, 1061
AfiI CCNNNNNNNGG 3 cut(s) 43, 317, 492
AgsI TTSAA 7 cut(s) 418, 580, 743, 877, 910, 957, 964
AluBI AGCT 7 cut(s) 51, 115, 127, 476, 701, 818, 953
AluI AGCT 7 cut(s) 51, 115, 127, 476, 701, 818, 953
Alw21I GWGCWC 2 cut(s) 279, 869
AlwI GGATC 3 cut(s) 228, 436, 503
Ama87I CYCGRG 1 cut(s) 99
AoxI GGCC 1 cut(s) 190
ApeKI GCWGC 4 cut(s) 140, 548, 623, 698
ApoI RAATTY 3 cut(s) 596, 846, 877
ArsI GACNNNNNNTTYG 2 cut(s) 217, 249
AseI ATTAAT 1 cut(s) 1115
AsuC2I CCSGG 5 cut(s) 100, 101, 218, 635, 976
AsuHPI GGTGA 2 cut(s) 146, 332
AsuII TTCGAA 1 cut(s) 844
AvaI CYCGRG 1 cut(s) 99
BanII GRGCYC 1 cut(s) 981
Bbv12I GWGCWC 2 cut(s) 279, 869
BbvI GCAGC 4 cut(s) 127, 560, 610, 710
BccI CCATC 6 cut(s) 36, 67, 125, 359, 605, 609
BcgI CGANNNNNNTGC 2 cut(s) 152, 186
BcnI CCSGG 5 cut(s) 100, 101, 218, 635, 976
BfaI CTAG 2 cut(s) 120, 180
BfuAI ACCTGC 1 cut(s) 2
BisI GCNGC 6 cut(s) 141, 213, 246, 549, 624, 699
BlsI GCNGC 6 cut(s) 142, 214, 247, 550, 625, 700
Bme1390I CCNGG 5 cut(s) 100, 101, 218, 635, 976
BmeT110I CYCGRG 1 cut(s) 99
BmiI GGNNCC 2 cut(s) 639, 980
BmrFI CCNGG 5 cut(s) 100, 101, 218, 635, 976
BmrI ACTGGG 1 cut(s) 1072
BmsI GCATC 4 cut(s) 543, 676, 717, 1089
BmuI ACTGGG 1 cut(s) 1072
Bpu14I TTCGAA 1 cut(s) 844
BpuEI CTTGAG 1 cut(s) 293
BpuMI CCSGG 5 cut(s) 100, 101, 218, 635, 976
BsaAI YACGTR 1 cut(s) 1059
BsaBI GATNNNNATC 1 cut(s) 1071
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 99, 216
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 317, 492
Bse1I ACTGG 2 cut(s) 664, 1067
Bse8I GATNNNNATC 1 cut(s) 1071
BseDI CCNNGG 2 cut(s) 99, 216
BseGI GGATG 3 cut(s) 28, 136, 859
BseJI GATNNNNATC 1 cut(s) 1071
BseLI CCNNNNNNNGG 3 cut(s) 43, 317, 492
BseMII CTCAG 3 cut(s) 78, 125, 1036
BseNI ACTGG 2 cut(s) 664, 1067
BseRI GAGGAG 1 cut(s) 633
BseXI GCAGC 4 cut(s) 127, 560, 610, 710
BshFI GGCC 1 cut(s) 192
BsiHKAI GWGCWC 2 cut(s) 279, 869
BsiHKCI CYCGRG 1 cut(s) 99
BsiSI CCGG 5 cut(s) 100, 218, 512, 635, 975
BslFI GGGAC 3 cut(s) 116, 190, 219
BslI CCNNNNNNNGG 3 cut(s) 43, 317, 492
BsmFI GGGAC 3 cut(s) 116, 190, 219
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 1 cut(s) 192
BsoBI CYCGRG 1 cut(s) 99
Bsp119I TTCGAA 1 cut(s) 844
Bsp1286I GDGCHC 3 cut(s) 279, 869, 981
Bsp143I GATC 3 cut(s) 220, 441, 508
BspACI CCGC 4 cut(s) 213, 245, 585, 615
BspANI GGCC 1 cut(s) 192
BspCNI CTCAG 3 cut(s) 77, 124, 1035
BspLI GGNNCC 2 cut(s) 639, 980
BspMI ACCTGC 1 cut(s) 2
BspPI GGATC 3 cut(s) 228, 436, 503
BspT104I TTCGAA 1 cut(s) 844
BsrI ACTGG 2 cut(s) 664, 1067
BssECI CCNNGG 2 cut(s) 99, 216
BssMI GATC 3 cut(s) 220, 441, 508
BssNAI GTATAC 1 cut(s) 349
BssNI GRCGYC 1 cut(s) 324
Bst1107I GTATAC 1 cut(s) 349
Bst4CI ACNGT 5 cut(s) 60, 233, 315, 522, 939
Bst6I CTCTTC 1 cut(s) 1008
BstACI GRCGYC 1 cut(s) 324
BstBAI YACGTR 1 cut(s) 1059
BstBI TTCGAA 1 cut(s) 844
BstC8I GCNNGC 2 cut(s) 210, 732
BstDEI CTNAG 5 cut(s) 64, 111, 328, 702, 1022
BstF5I GGATG 3 cut(s) 28, 136, 859
BstKTI GATC 3 cut(s) 223, 444, 511
BstMBI GATC 3 cut(s) 220, 441, 508
BstMWI GCNNNNNNNGC 5 cut(s) 149, 189, 209, 695, 985
BstSCI CCNGG 5 cut(s) 98, 99, 216, 633, 974
BstSNI TACGTA 1 cut(s) 1059
BstV1I GCAGC 4 cut(s) 127, 560, 610, 710
BstX2I RGATCY 1 cut(s) 508
BstYI RGATCY 1 cut(s) 508
BstZ17I GTATAC 1 cut(s) 349
BsuRI GGCC 1 cut(s) 192
BtsCI GGATG 3 cut(s) 28, 136, 859
BtsI GCAGTG 2 cut(s) 198, 277
BtsIMutI CAGTG 6 cut(s) 56, 198, 277, 311, 765, 935
BveI ACCTGC 1 cut(s) 2
Cac8I GCNNGC 2 cut(s) 210, 732
Cfr9I CCCGGG 1 cut(s) 99
CseI GACGC 1 cut(s) 332
Csp6I GTAC 6 cut(s) 657, 682, 761, 1030, 1056, 1060
CviAII CATG 5 cut(s) 43, 205, 720, 784, 983
CviQI GTAC 6 cut(s) 657, 682, 761, 1030, 1056, 1060
DdeI CTNAG 5 cut(s) 64, 111, 328, 702, 1022
DpnI GATC 3 cut(s) 222, 443, 510
DpnII GATC 3 cut(s) 220, 441, 508
Eam1104I CTCTTC 1 cut(s) 1008
EarI CTCTTC 1 cut(s) 1008
Eco105I TACGTA 1 cut(s) 1059
Eco24I GRGCYC 1 cut(s) 981
Eco57I CTGAAG 2 cut(s) 528, 789
Eco88I CYCGRG 1 cut(s) 99
EcoRI GAATTC 3 cut(s) 596, 846, 877
EcoT22I ATGCAT 1 cut(s) 536
EcoT38I GRGCYC 1 cut(s) 981
FaeI CATG 5 cut(s) 46, 208, 723, 787, 986
FaqI GGGAC 3 cut(s) 116, 190, 219
FatI CATG 5 cut(s) 42, 204, 719, 783, 982
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 6 cut(s) 141, 213, 246, 549, 624, 699
FokI GGATG 3 cut(s) 15, 143, 866
FriOI GRGCYC 1 cut(s) 981
Fsp4HI GCNGC 6 cut(s) 141, 213, 246, 549, 624, 699
FspBI CTAG 2 cut(s) 120, 180
GluI GCNGC 6 cut(s) 141, 213, 246, 549, 624, 699
HaeIII GGCC 1 cut(s) 192
HapII CCGG 5 cut(s) 100, 218, 512, 635, 975
HgaI GACGC 1 cut(s) 332
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 5 cut(s) 46, 208, 723, 787, 986
HindIII AAGCTT 1 cut(s) 951
HinfI GANTC 2 cut(s) 83, 104
HpaII CCGG 5 cut(s) 100, 218, 512, 635, 975
HphI GGTGA 2 cut(s) 146, 332
Hpy166II GTNNAC 1 cut(s) 349
Hpy188I TCNGA 4 cut(s) 526, 539, 829, 1025
Hpy188III TCNNGA 1 cut(s) 851
Hpy8I GTNNAC 1 cut(s) 349
HpyAV CCTTC 2 cut(s) 851, 1020
HpyCH4III ACNGT 5 cut(s) 60, 233, 315, 522, 939
HpyCH4IV ACGT 2 cut(s) 997, 1058
HpyCH4V TGCA 6 cut(s) 17, 143, 173, 338, 534, 730
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 189, 209, 695, 985
HpyF3I CTNAG 5 cut(s) 64, 111, 328, 702, 1022
HpySE526I ACGT 2 cut(s) 997, 1058
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 5 cut(s) 46, 208, 723, 787, 986
Kzo9I GATC 3 cut(s) 220, 441, 508
LmnI GCTCC 2 cut(s) 358, 984
Lsp1109I GCAGC 4 cut(s) 127, 560, 610, 710
LweI GCATC 4 cut(s) 543, 676, 717, 1089
MaeI CTAG 2 cut(s) 120, 180
MaeII ACGT 2 cut(s) 997, 1058
MaeIII GTNAC 4 cut(s) 196, 320, 779, 970
MalI GATC 3 cut(s) 222, 443, 510
MboI GATC 3 cut(s) 220, 441, 508
MboII GAAGA 3 cut(s) 472, 554, 1025
MfeI CAATTG 1 cut(s) 860
MflI RGATCY 1 cut(s) 508
MhlI GDGCHC 3 cut(s) 279, 869, 981
MluCI AATT 8 cut(s) 398, 503, 596, 846, 860, 877, 1044, 1116
MlyI GAGTC 1 cut(s) 98
MmeI TCCRAC 2 cut(s) 493, 504
Mph1103I ATGCAT 1 cut(s) 536
MseI TTAA 3 cut(s) 941, 1115, 1135
MslI CAYNNNNRTG 1 cut(s) 203
MspI CCGG 5 cut(s) 100, 218, 512, 635, 975
MspR9I CCNGG 5 cut(s) 100, 101, 218, 635, 976
MunI CAATTG 1 cut(s) 860
Mva1269I GAATGC 1 cut(s) 17
MwoI GCNNNNNNNGC 5 cut(s) 149, 189, 209, 695, 985
NciI CCSGG 5 cut(s) 100, 101, 218, 635, 976
NdeII GATC 3 cut(s) 220, 441, 508
NlaIII CATG 5 cut(s) 46, 208, 723, 787, 986
NlaIV GGNNCC 2 cut(s) 639, 980
NmuCI GTSAC 3 cut(s) 196, 320, 779
NsiI ATGCAT 1 cut(s) 536
NspV TTCGAA 1 cut(s) 844
PaqCI CACCTGC 1 cut(s) 2
PctI GAATGC 1 cut(s) 17
PfeI GAWTC 1 cut(s) 83
PflMI CCANNNNNTGG 1 cut(s) 317
PkrI GCNGC 6 cut(s) 142, 214, 247, 550, 625, 700
PleI GAGTC 1 cut(s) 98
PpsI GAGTC 1 cut(s) 98
Ppu21I YACGTR 1 cut(s) 1059
PshBI ATTAAT 1 cut(s) 1115
PspN4I GGNNCC 2 cut(s) 639, 980
PsuI RGATCY 1 cut(s) 508
RsaI GTAC 6 cut(s) 658, 683, 762, 1031, 1057, 1061
RsaNI GTAC 6 cut(s) 657, 682, 761, 1030, 1056, 1060
RseI CAYNNNNRTG 1 cut(s) 203
SaqAI TTAA 3 cut(s) 941, 1115, 1135
SatI GCNGC 6 cut(s) 141, 213, 246, 549, 624, 699
Sau3AI GATC 3 cut(s) 220, 441, 508
SchI GAGTC 1 cut(s) 98
ScrFI CCNGG 5 cut(s) 100, 101, 218, 635, 976
SduI GDGCHC 3 cut(s) 279, 869, 981
SfaNI GCATC 4 cut(s) 543, 676, 717, 1089
SfuI TTCGAA 1 cut(s) 844
SmaI CCCGGG 1 cut(s) 101
SmiMI CAYNNNNRTG 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
SnaBI TACGTA 1 cut(s) 1059
Sse9I AATT 8 cut(s) 398, 503, 596, 846, 860, 877, 1044, 1116
SsiI CCGC 4 cut(s) 213, 245, 585, 615
SspI AATATT 2 cut(s) 291, 307
SspMI CTAG 2 cut(s) 120, 180
StyD4I CCNGG 5 cut(s) 98, 99, 216, 633, 974
TaaI ACNGT 5 cut(s) 60, 233, 315, 522, 939
TaiI ACGT 2 cut(s) 1000, 1061
TaqI TCGA 3 cut(s) 162, 261, 844
TasI AATT 8 cut(s) 398, 503, 596, 846, 860, 877, 1044, 1116
TatI WGTACW 1 cut(s) 760
TauI GCSGC 2 cut(s) 215, 248
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 3 cut(s) 941, 1115, 1135
Tru9I TTAA 3 cut(s) 941, 1115, 1135
TscAI CASTG 6 cut(s) 63, 205, 284, 318, 772, 942
TseFI GTSAC 3 cut(s) 196, 320, 779
TseI GCWGC 4 cut(s) 140, 548, 623, 698
Tsp45I GTSAC 3 cut(s) 196, 320, 779
TspDTI ATGAA 4 cut(s) 654, 1008, 1026, 1086
TspMI CCCGGG 1 cut(s) 99
TspRI CASTG 6 cut(s) 63, 205, 284, 318, 772, 942
Van91I CCANNNNNTGG 1 cut(s) 317
VspI ATTAAT 1 cut(s) 1115
XapI RAATTY 3 cut(s) 596, 846, 877
XcmI CCANNNNNNNNNTGG 1 cut(s) 608
XmaI CCCGGG 1 cut(s) 99
XmiI GTMKAC 1 cut(s) 348
XspI CTAG 2 cut(s) 120, 180
Zsp2I ATGCAT 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.