RchiOBHm_Chr6g0307501

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003463
Physical Location & Seq
Reverse (-)
65844512 .. 65846889
2378 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27645

Sequence Viewer

Length: 1170 bp
ATGGAAAGCTCATCACTGTTCTCAGCATTGATGGAGGTGGAATCAAAGGGATTATCCCCGGACTCTACTCAGCTTCCTAGAAAGCTGGATGGTGAGGCTGCAAGGTTGGCAGATTATTTCGATGTTATTGCAGGGACTAGCACAGGTGGCCTTGTCACTGCCATGCTTGCCGCCCCGGATCAAAACAACCGTCCCTTGTTTGCGGCTAACCAGATTGTCGATTTTTACCTTGAGCACTGCCCTAAAATATTCCCTCAAATCAATATTCCACTGTTTGGTGACGCCTTAGAAAATGCAAAGGGTGTATACGCTCCAAAATATGATGGCAAGTATTTACACAAACTTATCAAGGAAAAATTAGGAGATAGACGATTGAACCAGACATTGACCAATGTTGTGATCCCAACTTTTGATATAAGTTGCCTCCAACCAGCTATCTTCTCCACCTACGAGTTGAAAAGGAACAAGAAATTAGATCCGGCTCTACTGTCGGATATATGCATCGGAACTTCAGCAGCACCAACTTATCTTCCCTCGCATTATTTCAAAACCGCAACAAGAGAATTCCATCTCGTTGATGGCGGTGTGGCTGCTAATAACCCGGGTTTGACTGCTATAACCGAAGTGACAAAAGAAATCAAGAAGGGAAATACTGACTTCTCTCCGGTTGAGGCAGCTGATCAGGGTAATAGGTTCCTCCTCATATCTTTGGGTACTGGTTCATCAGCAAAATCCAAGTACGATGCCAGAGTAGCAGCTAAGTGGGGTATTATGGCATGGTTGATGCAAGCCAACTTTCAATGTCCATTGTTAGATGTACTCACTGAAGCAAGTAGTGACATGGTAGATTGCCACCTTTCTACCCTTTTCCAAGCTCTTTGGTCTGACGATAACTACCTTCGAATTCAGGATGACCAATTGAGCACCGACTTGAATTCTATGGATAATGCTACGCCCGAAAACTTGAAAAAACTTGTAAAAGTGGGAGAGGCACTGTTAAAGAAACAAGCTTCAAAGTTGAATATAGTTACCGGGCTCCATGAGCCTGATGAACGTCATATTACTAATGAAGAGGCTCTCAGAAGGGTAGCGGCAGTTCTCTCCAAAGAGAAGAAGGAACGTGCAATCAGATCAGCAGCACCACAAGCAAACCCAGCATCTGCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

389

Amino Acids

42.6

Weight (kDa)

6.03

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 35 - 201 9.2e-17 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 275
AccI GTMKAC 1 cut(s) 306
AciI CCGC 5 cut(s) 171, 203, 552, 582, 1091
AclWI GGATC 3 cut(s) 186, 394, 470
AcsI RAATTY 3 cut(s) 563, 903, 934
AcuI CTGAAG 2 cut(s) 495, 846
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 3 cut(s) 715, 740, 819
AfiI CCNNNNNNNGG 1 cut(s) 275
AgsI TTSAA 8 cut(s) 376, 457, 547, 800, 934, 967, 1014, 1021
AluBI AGCT 9 cut(s) 9, 73, 85, 434, 677, 758, 875, 1010, 1167
AluI AGCT 9 cut(s) 9, 73, 85, 434, 677, 758, 875, 1010, 1167
Alw21I GWGCWC 2 cut(s) 237, 926
AlwI GGATC 3 cut(s) 186, 394, 470
AlwNI CAGNNNCTG 1 cut(s) 1160
Ama87I CYCGRG 1 cut(s) 601
AoxI GGCC 1 cut(s) 148
ApeKI GCWGC 6 cut(s) 98, 515, 590, 674, 755, 1136
ApoI RAATTY 3 cut(s) 563, 903, 934
ArsI GACNNNNNNTTYG 2 cut(s) 175, 207
AsuC2I CCSGG 5 cut(s) 59, 176, 602, 603, 1033
AsuHPI GGTGA 2 cut(s) 104, 290
AsuII TTCGAA 1 cut(s) 901
AvaI CYCGRG 1 cut(s) 601
BanII GRGCYC 1 cut(s) 1038
Bbv12I GWGCWC 2 cut(s) 237, 926
BbvI GCAGC 6 cut(s) 85, 527, 577, 686, 767, 1148
BccI CCATC 5 cut(s) 25, 83, 317, 572, 576
BcgI CGANNNNNNTGC 2 cut(s) 110, 144
BclI TGATCA 1 cut(s) 679
BcnI CCSGG 5 cut(s) 59, 176, 602, 603, 1033
BfaI CTAG 2 cut(s) 78, 138
BisI GCNGC 9 cut(s) 99, 171, 204, 516, 591, 675, 756, 1092, 1137
BlsI GCNGC 9 cut(s) 100, 172, 205, 517, 592, 676, 757, 1093, 1138
Bme1390I CCNGG 5 cut(s) 59, 176, 602, 603, 1033
BmeT110I CYCGRG 1 cut(s) 601
BmiI GGNNCC 2 cut(s) 695, 1037
BmrFI CCNGG 5 cut(s) 59, 176, 602, 603, 1033
BmsI GCATC 4 cut(s) 510, 733, 774, 1166
Bpu14I TTCGAA 1 cut(s) 901
BpuEI CTTGAG 1 cut(s) 251
BpuMI CCSGG 5 cut(s) 59, 176, 602, 603, 1033
BsaHI GRCGYC 1 cut(s) 282
BsaJI CCNNGG 3 cut(s) 57, 174, 601
BsaWI WCCGGW 1 cut(s) 664
Bsc4I CCNNNNNNNGG 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 721
BseDI CCNNGG 3 cut(s) 57, 174, 601
BseGI GGATG 2 cut(s) 94, 916
BseLI CCNNNNNNNGG 1 cut(s) 275
BseMII CTCAG 3 cut(s) 36, 83, 1093
BseNI ACTGG 1 cut(s) 721
BseRI GAGGAG 1 cut(s) 689
BseXI GCAGC 6 cut(s) 85, 527, 577, 686, 767, 1148
BseYI CCCAGC 1 cut(s) 1153
BshFI GGCC 1 cut(s) 150
BsiHKAI GWGCWC 2 cut(s) 237, 926
BsiHKCI CYCGRG 1 cut(s) 601
BsiSI CCGG 6 cut(s) 59, 176, 479, 602, 665, 1032
BslFI GGGAC 2 cut(s) 148, 177
BslI CCNNNNNNNGG 1 cut(s) 275
BsmFI GGGAC 2 cut(s) 148, 177
BsnI GGCC 1 cut(s) 150
BsoBI CYCGRG 1 cut(s) 601
Bsp119I TTCGAA 1 cut(s) 901
Bsp1286I GDGCHC 3 cut(s) 237, 926, 1038
Bsp143I GATC 5 cut(s) 178, 399, 475, 679, 1130
BspACI CCGC 5 cut(s) 171, 203, 552, 582, 1091
BspANI GGCC 1 cut(s) 150
BspCNI CTCAG 3 cut(s) 35, 82, 1092
BspLI GGNNCC 2 cut(s) 695, 1037
BspPI GGATC 3 cut(s) 186, 394, 470
BspT104I TTCGAA 1 cut(s) 901
BsrI ACTGG 1 cut(s) 721
BssECI CCNNGG 3 cut(s) 57, 174, 601
BssMI GATC 5 cut(s) 178, 399, 475, 679, 1130
BssNAI GTATAC 1 cut(s) 307
BssNI GRCGYC 1 cut(s) 282
Bst1107I GTATAC 1 cut(s) 307
Bst4CI ACNGT 5 cut(s) 18, 191, 273, 489, 996
Bst6I CTCTTC 1 cut(s) 1065
BstACI GRCGYC 1 cut(s) 282
BstBI TTCGAA 1 cut(s) 901
BstC8I GCNNGC 3 cut(s) 168, 789, 1165
BstDEI CTNAG 5 cut(s) 22, 69, 286, 759, 1079
BstF5I GGATG 2 cut(s) 94, 916
BstKTI GATC 5 cut(s) 181, 402, 478, 682, 1133
BstMBI GATC 5 cut(s) 178, 399, 475, 679, 1130
BstMWI GCNNNNNNNGC 7 cut(s) 107, 147, 167, 752, 1042, 1145, 1154
BstSCI CCNGG 5 cut(s) 57, 174, 600, 601, 1031
BstV1I GCAGC 6 cut(s) 85, 527, 577, 686, 767, 1148
BstX2I RGATCY 1 cut(s) 475
BstYI RGATCY 1 cut(s) 475
BstZ17I GTATAC 1 cut(s) 307
BsuRI GGCC 1 cut(s) 150
BtsCI GGATG 2 cut(s) 94, 916
BtsI GCAGTG 2 cut(s) 156, 235
BtsIMutI CAGTG 6 cut(s) 14, 156, 235, 269, 822, 992
Cac8I GCNNGC 3 cut(s) 168, 789, 1165
CaiI CAGNNNCTG 1 cut(s) 1160
Cfr9I CCCGGG 1 cut(s) 601
CseI GACGC 1 cut(s) 290
Csp6I GTAC 3 cut(s) 714, 739, 818
CviAII CATG 4 cut(s) 163, 777, 841, 1040
CviQI GTAC 3 cut(s) 714, 739, 818
DdeI CTNAG 5 cut(s) 22, 69, 286, 759, 1079
DpnI GATC 5 cut(s) 180, 401, 477, 681, 1132
DpnII GATC 5 cut(s) 178, 399, 475, 679, 1130
Eam1104I CTCTTC 1 cut(s) 1065
EarI CTCTTC 1 cut(s) 1065
Eco24I GRGCYC 1 cut(s) 1038
Eco57I CTGAAG 2 cut(s) 495, 846
Eco88I CYCGRG 1 cut(s) 601
EcoRI GAATTC 3 cut(s) 563, 903, 934
EcoT22I ATGCAT 1 cut(s) 503
EcoT38I GRGCYC 1 cut(s) 1038
FaeI CATG 4 cut(s) 166, 780, 844, 1043
FaqI GGGAC 2 cut(s) 148, 177
FatI CATG 4 cut(s) 162, 776, 840, 1039
FbaI TGATCA 1 cut(s) 679
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 9 cut(s) 99, 171, 204, 516, 591, 675, 756, 1092, 1137
FokI GGATG 2 cut(s) 101, 923
FriOI GRGCYC 1 cut(s) 1038
Fsp4HI GCNGC 9 cut(s) 99, 171, 204, 516, 591, 675, 756, 1092, 1137
FspBI CTAG 2 cut(s) 78, 138
GluI GCNGC 9 cut(s) 99, 171, 204, 516, 591, 675, 756, 1092, 1137
GsaI CCCAGC 1 cut(s) 1157
HaeIII GGCC 1 cut(s) 150
HapII CCGG 6 cut(s) 59, 176, 479, 602, 665, 1032
HgaI GACGC 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 4 cut(s) 166, 780, 844, 1043
HindIII AAGCTT 1 cut(s) 1008
HinfI GANTC 2 cut(s) 41, 62
HpaII CCGG 6 cut(s) 59, 176, 479, 602, 665, 1032
HphI GGTGA 2 cut(s) 104, 290
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 5 cut(s) 493, 506, 886, 1082, 1130
Hpy188III TCNNGA 2 cut(s) 640, 908
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 4 cut(s) 637, 908, 1077, 1108
HpyCH4III ACNGT 5 cut(s) 18, 191, 273, 489, 996
HpyCH4IV ACGT 2 cut(s) 1054, 1120
HpyCH4V TGCA 6 cut(s) 101, 131, 296, 501, 787, 1124
HpyF10VI GCNNNNNNNGC 7 cut(s) 107, 147, 167, 752, 1042, 1145, 1154
HpyF3I CTNAG 5 cut(s) 22, 69, 286, 759, 1079
HpySE526I ACGT 2 cut(s) 1054, 1120
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 4 cut(s) 166, 780, 844, 1043
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 5 cut(s) 178, 399, 475, 679, 1130
LmnI GCTCC 2 cut(s) 316, 1041
Lsp1109I GCAGC 6 cut(s) 85, 527, 577, 686, 767, 1148
LweI GCATC 4 cut(s) 510, 733, 774, 1166
MaeI CTAG 2 cut(s) 78, 138
MaeII ACGT 2 cut(s) 1054, 1120
MaeIII GTNAC 5 cut(s) 154, 278, 625, 836, 1027
MalI GATC 5 cut(s) 180, 401, 477, 681, 1132
MboI GATC 5 cut(s) 178, 399, 475, 679, 1130
MboII GAAGA 4 cut(s) 430, 521, 1082, 1123
MfeI CAATTG 1 cut(s) 917
MflI RGATCY 1 cut(s) 475
MhlI GDGCHC 3 cut(s) 237, 926, 1038
MluCI AATT 6 cut(s) 356, 470, 563, 903, 917, 934
MlyI GAGTC 1 cut(s) 56
MmeI TCCRAC 2 cut(s) 451, 471
Mph1103I ATGCAT 1 cut(s) 503
MseI TTAA 1 cut(s) 998
MslI CAYNNNNRTG 1 cut(s) 161
MspA1I CMGCKG 2 cut(s) 677, 1167
MspI CCGG 6 cut(s) 59, 176, 479, 602, 665, 1032
MspR9I CCNGG 5 cut(s) 59, 176, 602, 603, 1033
MunI CAATTG 1 cut(s) 917
MwoI GCNNNNNNNGC 7 cut(s) 107, 147, 167, 752, 1042, 1145, 1154
NciI CCSGG 5 cut(s) 59, 176, 602, 603, 1033
NdeII GATC 5 cut(s) 178, 399, 475, 679, 1130
NlaIII CATG 4 cut(s) 166, 780, 844, 1043
NlaIV GGNNCC 2 cut(s) 695, 1037
NmuCI GTSAC 4 cut(s) 154, 278, 625, 836
NsiI ATGCAT 1 cut(s) 503
NspV TTCGAA 1 cut(s) 901
PfeI GAWTC 1 cut(s) 41
PflMI CCANNNNNTGG 1 cut(s) 275
PkrI GCNGC 9 cut(s) 100, 172, 205, 517, 592, 676, 757, 1093, 1138
PleI GAGTC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 56
PspFI CCCAGC 1 cut(s) 1153
PspN4I GGNNCC 2 cut(s) 695, 1037
PsrI GAACNNNNNNTAC 2 cut(s) 1080, 1112
PstNI CAGNNNCTG 1 cut(s) 1160
PsuI RGATCY 1 cut(s) 475
PvuII CAGCTG 2 cut(s) 677, 1167
RsaI GTAC 3 cut(s) 715, 740, 819
RsaNI GTAC 3 cut(s) 714, 739, 818
RseI CAYNNNNRTG 1 cut(s) 161
SaqAI TTAA 1 cut(s) 998
SatI GCNGC 9 cut(s) 99, 171, 204, 516, 591, 675, 756, 1092, 1137
Sau3AI GATC 5 cut(s) 178, 399, 475, 679, 1130
SchI GAGTC 1 cut(s) 56
ScrFI CCNGG 5 cut(s) 59, 176, 602, 603, 1033
SduI GDGCHC 3 cut(s) 237, 926, 1038
SfaNI GCATC 4 cut(s) 510, 733, 774, 1166
SfuI TTCGAA 1 cut(s) 901
SmaI CCCGGG 1 cut(s) 603
SmiMI CAYNNNNRTG 1 cut(s) 161
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 6 cut(s) 356, 470, 563, 903, 917, 934
SsiI CCGC 5 cut(s) 171, 203, 552, 582, 1091
SspI AATATT 2 cut(s) 249, 265
SspMI CTAG 2 cut(s) 78, 138
StyD4I CCNGG 5 cut(s) 57, 174, 600, 601, 1031
TaaI ACNGT 5 cut(s) 18, 191, 273, 489, 996
TaiI ACGT 2 cut(s) 1057, 1123
TaqI TCGA 3 cut(s) 120, 219, 901
TasI AATT 6 cut(s) 356, 470, 563, 903, 917, 934
TatI WGTACW 1 cut(s) 817
TauI GCSGC 3 cut(s) 173, 206, 1094
TfiI GAWTC 1 cut(s) 41
Tru1I TTAA 1 cut(s) 998
Tru9I TTAA 1 cut(s) 998
TscAI CASTG 6 cut(s) 21, 163, 242, 276, 829, 999
TseFI GTSAC 4 cut(s) 154, 278, 625, 836
TseI GCWGC 6 cut(s) 98, 515, 590, 674, 755, 1136
Tsp45I GTSAC 4 cut(s) 154, 278, 625, 836
TspDTI ATGAA 3 cut(s) 711, 1065, 1083
TspMI CCCGGG 1 cut(s) 601
TspRI CASTG 6 cut(s) 21, 163, 242, 276, 829, 999
Van91I CCANNNNNTGG 1 cut(s) 275
XapI RAATTY 3 cut(s) 563, 903, 934
XcmI CCANNNNNNNNNTGG 1 cut(s) 575
XmaI CCCGGG 1 cut(s) 601
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 2 cut(s) 78, 138
Zsp2I ATGCAT 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.