Rh3BG286900

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0003463
Physical Location & Seq
Forward (+)
28642855 .. 28643719
865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG286900.1

Sequence Viewer

Length: 528 bp
ATGCTTACTGCCCCGGATCAAAACAACCGTCCCTTGTTTGCGGCTAACCAGATTGTCGATTTTTACCTTGAGCACTGCCCTAAAATATTCCCTCAAATCAATATTCCACTGTTTGGTAACGCCTTAGAAAATGCAAAGGGTGTATACGCACCAAAATATGATGGCAAGTATTTACACAAGCTTATCAAGGAAAAATTAGGAGACAGACAATTGAACCAGACATTGACCAATGTTGTGATCCCAACTTTTGATATAAATTGCCTCCAACCAGCTATCTTCTCCACCTACGAGTTGAAAAGGAACAAGAAATTAGATCCGGCTGTACTGTCGGATATATGCATCGGAACTTCAGCAGCACCAACTTATCTTGCCTCGCATTATTTCAAAACCGCAACAAGAGAATTCCATCTCGTTGATGGCAGTGTGGCTGCTAATAACCCTGGTTTGACTGCTATAACCGAAGTGACAAAAGAAATCAAGAAGGGAAATTCTGACTTCTCTTCGGTTGAGGCAGCTGATCAGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

175

Amino Acids

19.33

Weight (kDa)

6.89

Isoelectric Point (pI)

28.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 45 - 147 1.9e-06 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 113
AccI GTMKAC 1 cut(s) 144
AciI CCGC 2 cut(s) 41, 390
AclWI GGATC 3 cut(s) 24, 232, 308
AcsI RAATTY 2 cut(s) 401, 487
AcuI CTGAAG 1 cut(s) 333
AfaI GTAC 1 cut(s) 324
AfiI CCNNNNNNNGG 1 cut(s) 113
AgsI TTSAA 3 cut(s) 214, 295, 385
AjnI CCWGG 1 cut(s) 439
AluBI AGCT 3 cut(s) 181, 272, 515
AluI AGCT 3 cut(s) 181, 272, 515
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 3 cut(s) 24, 232, 308
ApeKI GCWGC 3 cut(s) 353, 428, 512
ApoI RAATTY 2 cut(s) 401, 487
ArsI GACNNNNNNTTYG 2 cut(s) 13, 45
AsuC2I CCSGG 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 75
BbvI GCAGC 3 cut(s) 365, 415, 524
BccI CCATC 3 cut(s) 155, 410, 414
BciT130I CCWGG 1 cut(s) 441
BclI TGATCA 1 cut(s) 517
BcnI CCSGG 1 cut(s) 14
BcoDI GTCTC 1 cut(s) 195
BisI GCNGC 4 cut(s) 42, 354, 429, 513
BlsI GCNGC 4 cut(s) 43, 355, 430, 514
Bme1390I CCNGG 2 cut(s) 14, 441
BmrFI CCNGG 2 cut(s) 14, 441
BmsI GCATC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 12, 439
Bsc4I CCNNNNNNNGG 1 cut(s) 113
BseBI CCWGG 1 cut(s) 441
BseDI CCNNGG 2 cut(s) 12, 439
BseLI CCNNNNNNNGG 1 cut(s) 113
BseXI GCAGC 3 cut(s) 365, 415, 524
BsiHKAI GWGCWC 1 cut(s) 75
BsiSI CCGG 2 cut(s) 14, 317
BslFI GGGAC 1 cut(s) 15
BslI CCNNNNNNNGG 1 cut(s) 113
BsmAI GTCTC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 4 cut(s) 16, 237, 313, 517
BspACI CCGC 2 cut(s) 41, 390
BspPI GGATC 3 cut(s) 24, 232, 308
BssECI CCNNGG 2 cut(s) 12, 439
BssMI GATC 4 cut(s) 16, 237, 313, 517
BssNAI GTATAC 1 cut(s) 145
Bst1107I GTATAC 1 cut(s) 145
Bst2UI CCWGG 1 cut(s) 441
Bst4CI ACNGT 3 cut(s) 29, 111, 327
Bst6I CTCTTC 1 cut(s) 505
BstDEI CTNAG 1 cut(s) 124
BstKTI GATC 4 cut(s) 19, 240, 316, 520
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 4 cut(s) 16, 237, 313, 517
BstNI CCWGG 1 cut(s) 441
BstSCI CCNGG 2 cut(s) 12, 439
BstV1I GCAGC 3 cut(s) 365, 415, 524
BstX2I RGATCY 1 cut(s) 313
BstYI RGATCY 1 cut(s) 313
BstZ17I GTATAC 1 cut(s) 145
BtsI GCAGTG 2 cut(s) 73, 427
BtsIMutI CAGTG 3 cut(s) 73, 107, 427
Csp6I GTAC 1 cut(s) 323
CviJI RGCY 6 cut(s) 44, 181, 272, 320, 428, 515
CviKI_1 RGCY 6 cut(s) 44, 181, 272, 320, 428, 515
CviQI GTAC 1 cut(s) 323
DdeI CTNAG 1 cut(s) 124
DpnI GATC 4 cut(s) 18, 239, 315, 519
DpnII GATC 4 cut(s) 16, 237, 313, 517
Eam1104I CTCTTC 1 cut(s) 505
EarI CTCTTC 1 cut(s) 505
Eco57I CTGAAG 1 cut(s) 333
EcoRI GAATTC 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 439
EcoT22I ATGCAT 1 cut(s) 341
FaiI YATR 6 cut(s) 145, 159, 254, 335, 337, 455
FaqI GGGAC 1 cut(s) 15
FbaI TGATCA 1 cut(s) 517
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 4 cut(s) 42, 354, 429, 513
Fsp4HI GCNGC 4 cut(s) 42, 354, 429, 513
GluI GCNGC 4 cut(s) 42, 354, 429, 513
HapII CCGG 2 cut(s) 14, 317
HindIII AAGCTT 1 cut(s) 179
HpaII CCGG 2 cut(s) 14, 317
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 3 cut(s) 331, 344, 493
Hpy188III TCNNGA 1 cut(s) 478
Hpy8I GTNNAC 1 cut(s) 145
HpyAV CCTTC 1 cut(s) 475
HpyCH4III ACNGT 3 cut(s) 29, 111, 327
HpyCH4V TGCA 2 cut(s) 134, 339
HpyF3I CTNAG 1 cut(s) 124
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 4 cut(s) 16, 237, 313, 517
LpnPI CCDG 8 cut(s) 27, 62, 230, 282, 330, 426, 453, 506
Lsp1109I GCAGC 3 cut(s) 365, 415, 524
LweI GCATC 1 cut(s) 348
MaeIII GTNAC 2 cut(s) 116, 463
MalI GATC 4 cut(s) 18, 239, 315, 519
MboI GATC 4 cut(s) 16, 237, 313, 517
MboII GAAGA 2 cut(s) 268, 492
MfeI CAATTG 1 cut(s) 209
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 6 cut(s) 194, 209, 256, 308, 401, 487
MmeI TCCRAC 2 cut(s) 289, 309
MnlI CCTC 4 cut(s) 102, 272, 382, 502
Mph1103I ATGCAT 1 cut(s) 341
MspA1I CMGCKG 1 cut(s) 515
MspI CCGG 2 cut(s) 14, 317
MspR9I CCNGG 2 cut(s) 14, 441
MunI CAATTG 1 cut(s) 209
MvaI CCWGG 1 cut(s) 441
NciI CCSGG 1 cut(s) 14
NdeII GATC 4 cut(s) 16, 237, 313, 517
NmuCI GTSAC 1 cut(s) 463
NsiI ATGCAT 1 cut(s) 341
PflMI CCANNNNNTGG 1 cut(s) 113
PkrI GCNGC 4 cut(s) 43, 355, 430, 514
Psp6I CCWGG 1 cut(s) 439
PspGI CCWGG 1 cut(s) 439
PsuI RGATCY 1 cut(s) 313
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 1 cut(s) 324
RsaNI GTAC 1 cut(s) 323
SatI GCNGC 4 cut(s) 42, 354, 429, 513
Sau3AI GATC 4 cut(s) 16, 237, 313, 517
ScrFI CCNGG 2 cut(s) 14, 441
SduI GDGCHC 1 cut(s) 75
SetI ASST 5 cut(s) 69, 183, 274, 287, 517
SfaNI GCATC 1 cut(s) 348
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 6 cut(s) 194, 209, 256, 308, 401, 487
SsiI CCGC 2 cut(s) 41, 390
SspI AATATT 2 cut(s) 87, 103
StyD4I CCNGG 2 cut(s) 12, 439
TaaI ACNGT 3 cut(s) 29, 111, 327
TaqI TCGA 1 cut(s) 57
TasI AATT 6 cut(s) 194, 209, 256, 308, 401, 487
TatI WGTACW 1 cut(s) 322
TauI GCSGC 1 cut(s) 44
TscAI CASTG 3 cut(s) 80, 114, 427
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 3 cut(s) 353, 428, 512
Tsp45I GTSAC 1 cut(s) 463
TspRI CASTG 3 cut(s) 80, 114, 427
Van91I CCANNNNNTGG 1 cut(s) 113
XapI RAATTY 2 cut(s) 401, 487
XcmI CCANNNNNNNNNTGG 1 cut(s) 413
XmiI GTMKAC 1 cut(s) 144
Zsp2I ATGCAT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.