MD08G1100500.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003463
Physical Location & Seq
Forward (+)
8442269 .. 8446188
3920 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1100500.v1.1.491

Sequence Viewer

Length: 1170 bp
ATGGCAAAGTCATCCCTTCACTCTACCACTAATGGAAGCCTCATCACTGTTCTTAGCATTGATGGAGGTGGAATAAGAGGGATTATTCCTGGAACTATACTCAGTTTTCTGGAGTCTGAGCTTCAGAAACTGGATGGTAAGGATGCAAGGCTCGCAGATTATTTTGATGTGATTACAGGGACGAGCACAGGTGGCCTTGTGACTGCCATGCTTACCACCCCAGATGAAAATAAGCGTCCCTTGTATGCTGCTAAAGATATTACCGCTTTTTATCTTGAAAACTGCCCTAAAATTTTCCCTCAGGACAGTTCTATTGTTGCCAACTTGAAAGTTTTGGGAAAACCAAAATATGATGGGAAGTATTTACATTCCCTATTAAAGGAAAAGTTGGGAGACAAGCACTTGCGCCACACCTTGACTAACGTTGTAATCCCAACTTTTGATATAAAGCGGCTGCAACCAGTTATCTTCTCCAGCTATCAGTTGAAAAAGAACAATAGCCTAGATGCTTTGCTATCGGATATATGTATCGGAACTTCAGCAGCCCCGTACTACCTTCCAGCATATCAATTTAAATCGACTAATTCATCTGGTGAAACAAGAGAATTCAATCTCACTGATGGTGGTGTGGCTGCTAATAACCCGGCTTTGGTTGCTATGAGCGAAGTGACAAAACAAATCCAGGGCAACCCTGACTTTGTTCCTTTGGGCGCAAATGACAAGTTGTATGAGCGGTTCCTAGTTATATCACTGGGTACCGGTACAACATCTGAGGAGAAGTACGATGCCAAAGAAGCATCTGAGTGGGGTGCTTTGGGATGGTTGATTGGATCTGATTTTTCAGCTCCTCTGGTAGATATATTCAGTCAAGCAAGTAGTGACATGGTTGATTTCCACCTTGCTACCGTTTTCCAAGCACTTGGGTCTCCCGACAATTATCTTCGAATTCAGGATGACACACTGAGTGGAACTCTGGCTTCTGTGGATATTGCTACTTCAGAAAACTTGAATGATCTCGTAAAAGTTGGGGAGAAATTGTTAACGAAACCAGTTTCAAGAGTGGACTTAGATACTGGAAAAGCAAAGCCTGTTCATCCTGAAATTACAAATCAAGAGGCACTCATAAGGTATTTCAATATTGCATACATACATCACCAAATCTACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

390

Amino Acids

42.43

Weight (kDa)

5.43

Isoelectric Point (pI)

26.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 18 - 220 4e-26 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 755
AccB1I GGYRCC 1 cut(s) 755
AccBSI CCGCTC 1 cut(s) 733
AciI CCGC 3 cut(s) 264, 451, 733
AclI AACGTT 1 cut(s) 423
AclWI GGATC 1 cut(s) 838
AcsI RAATTY 3 cut(s) 291, 605, 945
AcuI CTGAAG 3 cut(s) 107, 522, 981
AdeI CACNNNGTG 1 cut(s) 965
AfaI GTAC 4 cut(s) 551, 757, 763, 782
AfiI CCNNNNNNNGG 2 cut(s) 379, 649
AgeI ACCGGT 1 cut(s) 758
AgsI TTSAA 7 cut(s) 278, 328, 487, 610, 1009, 1056, 1135
AjnI CCWGG 2 cut(s) 88, 681
AluBI AGCT 3 cut(s) 121, 477, 845
AluI AGCT 3 cut(s) 121, 477, 845
Alw21I GWGCWC 1 cut(s) 188
Alw26I GTCTC 2 cut(s) 387, 930
AlwI GGATC 1 cut(s) 838
AlwNI CAGNNNCTG 1 cut(s) 130
AoxI GGCC 1 cut(s) 193
ApeKI GCWGC 4 cut(s) 248, 454, 542, 632
ApoI RAATTY 3 cut(s) 291, 605, 945
AsiGI ACCGGT 1 cut(s) 758
Asp718I GGTACC 1 cut(s) 755
AspLEI GCGC 2 cut(s) 408, 713
AsuC2I CCSGG 1 cut(s) 644
AsuHPI GGTGA 2 cut(s) 605, 1145
AsuII TTCGAA 1 cut(s) 943
AxyI CCTNAGG 1 cut(s) 300
BanI GGYRCC 1 cut(s) 755
BarI GAAGNNNNNNTAC 2 cut(s) 520, 552
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 4 cut(s) 235, 441, 554, 619
BccI CCATC 5 cut(s) 56, 128, 347, 614, 813
BciT130I CCWGG 2 cut(s) 90, 683
BcnI CCSGG 1 cut(s) 644
BcoDI GTCTC 2 cut(s) 387, 930
BfaI CTAG 2 cut(s) 503, 740
BisI GCNGC 5 cut(s) 249, 452, 455, 543, 633
BlsI GCNGC 5 cut(s) 250, 453, 456, 544, 634
Bme1390I CCNGG 3 cut(s) 90, 644, 683
BmiI GGNNCC 2 cut(s) 737, 757
BmrFI CCNGG 3 cut(s) 90, 644, 683
BmrI ACTGGG 1 cut(s) 761
BmsI GCATC 4 cut(s) 133, 496, 775, 806
BmuI ACTGGG 1 cut(s) 761
BplI GAGNNNNNCTC 2 cut(s) 955, 987
BpmI CTGGAG 2 cut(s) 131, 457
Bpu14I TTCGAA 1 cut(s) 943
BpuMI CCSGG 1 cut(s) 644
BsaI GGTCTC 1 cut(s) 930
BsaJI CCNNGG 1 cut(s) 682
BsaWI WCCGGW 1 cut(s) 758
BsaXI ACNNNNNCTCC 2 cut(s) 1022, 1052
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 649
Bse118I RCCGGY 1 cut(s) 758
Bse1I ACTGG 5 cut(s) 135, 461, 756, 1049, 1078
Bse21I CCTNAGG 1 cut(s) 300
BseBI CCWGG 2 cut(s) 90, 683
BseDI CCNNGG 1 cut(s) 682
BseGI GGATG 6 cut(s) 11, 139, 148, 824, 958, 1093
BseLI CCNNNNNNNGG 2 cut(s) 379, 649
BseMII CTCAG 6 cut(s) 108, 115, 314, 762, 792, 953
BseNI ACTGG 5 cut(s) 135, 461, 756, 1049, 1078
BseRI GAGGAG 2 cut(s) 788, 837
BseXI GCAGC 4 cut(s) 235, 441, 554, 619
BshFI GGCC 1 cut(s) 195
BshNI GGYRCC 1 cut(s) 755
BshTI ACCGGT 1 cut(s) 758
BsiHKAI GWGCWC 1 cut(s) 188
BsiSI CCGG 2 cut(s) 644, 759
BslFI GGGAC 2 cut(s) 193, 222
BslI CCNNNNNNNGG 2 cut(s) 379, 649
BsmAI GTCTC 2 cut(s) 387, 930
BsmFI GGGAC 2 cut(s) 193, 222
BsnI GGCC 1 cut(s) 195
Bso31I GGTCTC 1 cut(s) 930
Bsp119I TTCGAA 1 cut(s) 943
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 2 cut(s) 830, 1012
BspACI CCGC 3 cut(s) 264, 451, 733
BspANI GGCC 1 cut(s) 195
BspCNI CTCAG 6 cut(s) 109, 114, 313, 763, 793, 954
BspLI GGNNCC 2 cut(s) 737, 757
BspPI GGATC 1 cut(s) 838
BspT104I TTCGAA 1 cut(s) 943
BspT107I GGYRCC 1 cut(s) 755
BspTNI GGTCTC 1 cut(s) 930
BsrBI CCGCTC 1 cut(s) 733
BsrFI RCCGGY 1 cut(s) 758
BsrI ACTGG 5 cut(s) 135, 461, 756, 1049, 1078
BssAI RCCGGY 1 cut(s) 758
BssECI CCNNGG 1 cut(s) 682
BssMI GATC 2 cut(s) 830, 1012
Bst2UI CCWGG 2 cut(s) 90, 683
Bst4CI ACNGT 3 cut(s) 49, 308, 907
BstBI TTCGAA 1 cut(s) 943
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 8 cut(s) 53, 101, 117, 300, 771, 801, 962, 1066
BstENI CCTNNNNNAGG 1 cut(s) 377
BstF5I GGATG 6 cut(s) 11, 139, 148, 824, 958, 1093
BstHHI GCGC 2 cut(s) 408, 713
BstKTI GATC 2 cut(s) 833, 1015
BstMAI GTCTC 2 cut(s) 387, 930
BstMBI GATC 2 cut(s) 830, 1012
BstMWI GCNNNNNNNGC 4 cut(s) 152, 192, 653, 794
BstNI CCWGG 2 cut(s) 90, 683
BstSCI CCNGG 3 cut(s) 88, 642, 681
BstV1I GCAGC 4 cut(s) 235, 441, 554, 619
BstX2I RGATCY 1 cut(s) 830
BstXI CCANNNNNNTGG 1 cut(s) 920
BstYI RGATCY 1 cut(s) 830
Bsu36I CCTNAGG 1 cut(s) 300
BsuRI GGCC 1 cut(s) 195
BtsCI GGATG 6 cut(s) 11, 139, 148, 824, 958, 1093
BtsIMutI CAGTG 4 cut(s) 45, 615, 749, 959
Cac8I GCNNGC 1 cut(s) 153
CaiI CAGNNNCTG 1 cut(s) 130
CfoI GCGC 2 cut(s) 408, 713
Cfr10I RCCGGY 1 cut(s) 758
CseI GACGC 1 cut(s) 224
Csp6I GTAC 4 cut(s) 550, 756, 762, 781
CspAI ACCGGT 1 cut(s) 758
CviAII CATG 2 cut(s) 208, 883
CviQI GTAC 4 cut(s) 550, 756, 762, 781
DdeI CTNAG 8 cut(s) 53, 101, 117, 300, 771, 801, 962, 1066
DpnI GATC 2 cut(s) 832, 1014
DpnII GATC 2 cut(s) 830, 1012
DraI TTTAAA 1 cut(s) 574
DraIII CACNNNGTG 1 cut(s) 965
Eco31I GGTCTC 1 cut(s) 930
Eco57I CTGAAG 3 cut(s) 107, 522, 981
Eco81I CCTNAGG 1 cut(s) 300
EcoNI CCTNNNNNAGG 1 cut(s) 377
EcoRI GAATTC 2 cut(s) 605, 945
EcoRII CCWGG 2 cut(s) 88, 681
FaeI CATG 2 cut(s) 211, 886
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FaqI GGGAC 2 cut(s) 193, 222
FatI CATG 2 cut(s) 207, 882
Fnu4HI GCNGC 5 cut(s) 249, 452, 455, 543, 633
FokI GGATG 5 cut(s) 146, 155, 831, 965, 1080
Fsp4HI GCNGC 5 cut(s) 249, 452, 455, 543, 633
FspBI CTAG 2 cut(s) 503, 740
GlaI GCGC 2 cut(s) 407, 712
GluI GCNGC 5 cut(s) 249, 452, 455, 543, 633
GsuI CTGGAG 2 cut(s) 131, 457
HaeIII GGCC 1 cut(s) 195
HapII CCGG 2 cut(s) 644, 759
HgaI GACGC 1 cut(s) 224
HhaI GCGC 2 cut(s) 408, 713
Hin1II CATG 2 cut(s) 211, 886
Hin6I GCGC 2 cut(s) 406, 711
HinP1I GCGC 2 cut(s) 406, 711
HincII GTYRAC 1 cut(s) 1041
HindII GTYRAC 1 cut(s) 1041
HinfI GANTC 1 cut(s) 113
HpaI GTTAAC 1 cut(s) 1041
HpaII CCGG 2 cut(s) 644, 759
HphI GGTGA 2 cut(s) 605, 1145
Hpy166II GTNNAC 2 cut(s) 1041, 1063
Hpy188I TCNGA 8 cut(s) 118, 126, 520, 533, 772, 802, 835, 1000
Hpy188III TCNNGA 8 cut(s) 110, 275, 302, 929, 950, 1056, 1097, 1112
Hpy8I GTNNAC 2 cut(s) 1041, 1063
HpyAV CCTTC 2 cut(s) 26, 566
HpyCH4III ACNGT 3 cut(s) 49, 308, 907
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 3 cut(s) 146, 457, 1142
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 192, 653, 794
HpyF3I CTNAG 8 cut(s) 53, 101, 117, 300, 771, 801, 962, 1066
HpySE526I ACGT 1 cut(s) 423
Hsp92II CATG 2 cut(s) 211, 886
HspAI GCGC 2 cut(s) 406, 711
KpnI GGTACC 1 cut(s) 759
KspAI GTTAAC 1 cut(s) 1041
Kzo9I GATC 2 cut(s) 830, 1012
LmnI GCTCC 1 cut(s) 850
Lsp1109I GCAGC 4 cut(s) 235, 441, 554, 619
LweI GCATC 4 cut(s) 133, 496, 775, 806
MaeI CTAG 2 cut(s) 503, 740
MaeII ACGT 1 cut(s) 423
MaeIII GTNAC 3 cut(s) 199, 667, 878
MalI GATC 2 cut(s) 832, 1014
MbiI CCGCTC 1 cut(s) 733
MboI GATC 2 cut(s) 830, 1012
MboII GAAGA 2 cut(s) 460, 932
MflI RGATCY 1 cut(s) 830
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 9 cut(s) 291, 569, 583, 605, 934, 945, 1034, 1101, 1165
MlyI GAGTC 1 cut(s) 122
MnlI CCTC 7 cut(s) 50, 59, 71, 309, 766, 858, 1108
MseI TTAA 4 cut(s) 377, 573, 1040, 1168
MslI CAYNNNNRTG 1 cut(s) 802
MspI CCGG 2 cut(s) 644, 759
MspR9I CCNGG 3 cut(s) 90, 644, 683
MvaI CCWGG 2 cut(s) 90, 683
MwoI GCNNNNNNNGC 4 cut(s) 152, 192, 653, 794
NciI CCSGG 1 cut(s) 644
NdeII GATC 2 cut(s) 830, 1012
NlaIII CATG 2 cut(s) 211, 886
NlaIV GGNNCC 2 cut(s) 737, 757
NmuCI GTSAC 3 cut(s) 199, 667, 878
NspV TTCGAA 1 cut(s) 943
PfoI TCCNGGA 1 cut(s) 88
PinAI ACCGGT 1 cut(s) 758
PkrI GCNGC 5 cut(s) 250, 453, 456, 544, 634
PleI GAGTC 1 cut(s) 121
PpsI GAGTC 1 cut(s) 121
Psp1406I AACGTT 1 cut(s) 423
Psp6I CCWGG 2 cut(s) 88, 681
PspGI CCWGG 2 cut(s) 88, 681
PspN4I GGNNCC 2 cut(s) 737, 757
PsrI GAACNNNNNNTAC 2 cut(s) 719, 751
PstNI CAGNNNCTG 1 cut(s) 130
PsuI RGATCY 1 cut(s) 830
RsaI GTAC 4 cut(s) 551, 757, 763, 782
RsaNI GTAC 4 cut(s) 550, 756, 762, 781
RseI CAYNNNNRTG 1 cut(s) 802
SaqAI TTAA 4 cut(s) 377, 573, 1040, 1168
SatI GCNGC 5 cut(s) 249, 452, 455, 543, 633
Sau3AI GATC 2 cut(s) 830, 1012
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 3 cut(s) 90, 644, 683
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 4 cut(s) 133, 496, 775, 806
SfuI TTCGAA 1 cut(s) 943
SmiI ATTTAAAT 1 cut(s) 574
SmiMI CAYNNNNRTG 1 cut(s) 802
Sse9I AATT 9 cut(s) 291, 569, 583, 605, 934, 945, 1034, 1101, 1165
SsiI CCGC 3 cut(s) 264, 451, 733
SspI AATATT 1 cut(s) 1138
SspMI CTAG 2 cut(s) 503, 740
StyD4I CCNGG 3 cut(s) 88, 642, 681
SwaI ATTTAAAT 1 cut(s) 574
TaaI ACNGT 3 cut(s) 49, 308, 907
TaiI ACGT 1 cut(s) 426
TaqI TCGA 2 cut(s) 578, 943
TasI AATT 9 cut(s) 291, 569, 583, 605, 934, 945, 1034, 1101, 1165
TauI GCSGC 1 cut(s) 454
Tru1I TTAA 4 cut(s) 377, 573, 1040, 1168
Tru9I TTAA 4 cut(s) 377, 573, 1040, 1168
TscAI CASTG 4 cut(s) 52, 622, 756, 966
TseFI GTSAC 3 cut(s) 199, 667, 878
TseI GCWGC 4 cut(s) 248, 454, 542, 632
Tsp45I GTSAC 3 cut(s) 199, 667, 878
TspDTI ATGAA 3 cut(s) 240, 576, 1082
TspRI CASTG 4 cut(s) 52, 622, 756, 966
XagI CCTNNNNNAGG 1 cut(s) 377
XapI RAATTY 3 cut(s) 291, 605, 945
XspI CTAG 2 cut(s) 503, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.