Rh6BG431100

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003463
Physical Location & Seq
Forward (+)
65060488 .. 65061504
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG431100.1

Sequence Viewer

Length: 753 bp
ATGAAAGGGCCAAAATATGATGGGGAGTATTTACATAAGATAGTGAGGGAAAGGTTGGGAGACATACGATTGAACGAGACATTGACTAACGTTGTAATCCCAACTTTTGATATAAAGAGGCTTCAGCCAGTCATCTTCTCCAGCTATGAGGTTAAGAAGAATGATAGCTTAGATGCTTTACTCTCCGACATATGCATTGGAACTTCAGCAGCCCCAACTTATCTCCCAGCGCATCATTTTGAAACCCAAAATTCCAGAACTGGAGAAGCAAGAGAATTCAATCTGATTGACGGTGCAGTCGCTGCTAATAATCCGGCTTTGGTCGCTATAAACGAAGTAACAAAAGAAATCCACCAAGGAAACACCGACTTCTTTCCTATGAGGCCAACTGAGTATGGGCGATTCCTCGTTATATCACTGGGTACTGGTTCAGGGAAATGCGAGGAGAAGTATGCCGCCGCAGATGCTGCTAAGTGGGGCGTTTTGGGGTGGCTAACCGCAGGTGGTTCATGTCCATTGGTTGACGTATTTACTCAAGCAAGTAGTGACATGGTCGACTTCCACCTGGCCACCGTTTTCCAAGCTCTTCATTCTGAGAAAAATTACCTTCGGATTCAAGACGATTCACTGGAGGGAACAGTGTCTTCAGTGGACATTGCTACGCATGAGAACTTGAACAACCTTGTAAAAGTTGGAGAGGAGTTGTTAAAGACACCGGTTTCAAGGGTGAATATGGAAACTGGTATATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

250

Amino Acids

27.52

Weight (kDa)

5.17

Isoelectric Point (pI)

25.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 3 - 110 4.8e-10 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 491
AasI GACNNNNNNGTC 1 cut(s) 296
Acc36I ACCTGC 1 cut(s) 491
AccI GTMKAC 1 cut(s) 555
AciI CCGC 3 cut(s) 456, 459, 498
AclI AACGTT 1 cut(s) 90
AcoI YGGCCR 1 cut(s) 567
AcsI RAATTY 2 cut(s) 250, 275
AcuI CTGAAG 3 cut(s) 107, 189, 630
AfaI GTAC 1 cut(s) 424
AgeI ACCGGT 1 cut(s) 715
AgsI TTSAA 6 cut(s) 73, 242, 280, 617, 676, 723
AjnI CCWGG 1 cut(s) 564
AluBI AGCT 3 cut(s) 144, 168, 584
AluI AGCT 3 cut(s) 144, 168, 584
Alw26I GTCTC 2 cut(s) 54, 71
AlwNI CAGNNNCTG 2 cut(s) 302, 467
AoxI GGCC 3 cut(s) 8, 383, 567
ApeKI GCWGC 3 cut(s) 209, 302, 467
ApoI RAATTY 2 cut(s) 250, 275
AsiGI ACCGGT 1 cut(s) 715
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 1 cut(s) 8
AsuHPI GGTGA 1 cut(s) 739
BalI TGGCCA 1 cut(s) 569
BbsI GAAGAC 1 cut(s) 636
BbvI GCAGC 3 cut(s) 221, 289, 454
BccI CCATC 1 cut(s) 14
BciT130I CCWGG 1 cut(s) 566
BcoDI GTCTC 2 cut(s) 54, 71
BfaI CTAG 1 cut(s) 751
BfuAI ACCTGC 1 cut(s) 491
BisI GCNGC 5 cut(s) 210, 303, 456, 459, 468
BlsI GCNGC 5 cut(s) 211, 304, 457, 460, 469
Bme1390I CCNGG 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 8
BmrFI CCNGG 1 cut(s) 566
BmrI ACTGGG 1 cut(s) 428
BmsI GCATC 3 cut(s) 163, 241, 454
BmuI ACTGGG 1 cut(s) 428
BpiI GAAGAC 1 cut(s) 636
BpmI CTGGAG 3 cut(s) 124, 282, 650
BpuEI CTTGAG 1 cut(s) 519
BsaJI CCNNGG 1 cut(s) 355
BsaWI WCCGGW 1 cut(s) 715
Bse118I RCCGGY 1 cut(s) 715
Bse1I ACTGG 6 cut(s) 128, 265, 423, 430, 633, 745
Bse3DI GCAATG 1 cut(s) 654
BseBI CCWGG 1 cut(s) 566
BseDI CCNNGG 1 cut(s) 355
BseMI GCAATG 1 cut(s) 654
BseMII CTCAG 2 cut(s) 381, 585
BseNI ACTGG 6 cut(s) 128, 265, 423, 430, 633, 745
BseRI GAGGAG 2 cut(s) 458, 713
BseXI GCAGC 3 cut(s) 221, 289, 454
BseYI CCCAGC 1 cut(s) 226
BsgI GTGCAG 1 cut(s) 315
BshFI GGCC 3 cut(s) 10, 385, 569
BshTI ACCGGT 1 cut(s) 715
BsiSI CCGG 2 cut(s) 314, 716
BsmAI GTCTC 2 cut(s) 54, 71
BsnI GGCC 3 cut(s) 10, 385, 569
BspACI CCGC 3 cut(s) 456, 459, 498
BspANI GGCC 3 cut(s) 10, 385, 569
BspCNI CTCAG 2 cut(s) 382, 586
BspMI ACCTGC 1 cut(s) 491
BspQI GCTCTTC 1 cut(s) 591
BsrDI GCAATG 1 cut(s) 654
BsrFI RCCGGY 1 cut(s) 715
BsrI ACTGG 6 cut(s) 128, 265, 423, 430, 633, 745
BssAI RCCGGY 1 cut(s) 715
BssECI CCNNGG 1 cut(s) 355
BssT1I CCWWGG 1 cut(s) 355
Bst2UI CCWGG 1 cut(s) 566
Bst4CI ACNGT 3 cut(s) 293, 574, 640
Bst6I CTCTTC 1 cut(s) 591
BstAPI GCANNNNNTGC 2 cut(s) 302, 467
BstDEI CTNAG 4 cut(s) 169, 390, 471, 594
BstHHI GCGC 1 cut(s) 232
BstMAI GTCTC 2 cut(s) 54, 71
BstMWI GCNNNNNNNGC 4 cut(s) 302, 323, 464, 467
BstNI CCWGG 1 cut(s) 566
BstSCI CCNGG 1 cut(s) 564
BstV1I GCAGC 3 cut(s) 221, 289, 454
BstV2I GAAGAC 1 cut(s) 636
BsuRI GGCC 3 cut(s) 10, 385, 569
BtsIMutI CAGTG 4 cut(s) 416, 626, 645, 654
BveI ACCTGC 1 cut(s) 491
CaiI CAGNNNCTG 2 cut(s) 302, 467
CfoI GCGC 1 cut(s) 232
Cfr10I RCCGGY 1 cut(s) 715
Cfr13I GGNCC 1 cut(s) 8
Csp6I GTAC 1 cut(s) 423
CspAI ACCGGT 1 cut(s) 715
CviAII CATG 3 cut(s) 510, 550, 665
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 4 cut(s) 169, 390, 471, 594
DrdI GACNNNNNNGTC 1 cut(s) 296
DseDI GACNNNNNNGTC 1 cut(s) 296
EaeI YGGCCR 1 cut(s) 567
Eam1104I CTCTTC 1 cut(s) 591
EarI CTCTTC 1 cut(s) 591
Eco130I CCWWGG 1 cut(s) 355
Eco57I CTGAAG 3 cut(s) 107, 189, 630
EcoRI GAATTC 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 564
EcoT14I CCWWGG 1 cut(s) 355
EcoT22I ATGCAT 1 cut(s) 197
ErhI CCWWGG 1 cut(s) 355
FaeI CATG 3 cut(s) 513, 553, 668
FatI CATG 3 cut(s) 509, 549, 664
FauNDI CATATG 1 cut(s) 191
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 5 cut(s) 210, 303, 456, 459, 468
Fsp4HI GCNGC 5 cut(s) 210, 303, 456, 459, 468
FspBI CTAG 1 cut(s) 751
GlaI GCGC 1 cut(s) 231
GluI GCNGC 5 cut(s) 210, 303, 456, 459, 468
GsaI CCCAGC 1 cut(s) 230
GsuI CTGGAG 3 cut(s) 124, 282, 650
HaeIII GGCC 3 cut(s) 10, 385, 569
HapII CCGG 2 cut(s) 314, 716
HhaI GCGC 1 cut(s) 232
Hin1II CATG 3 cut(s) 513, 553, 668
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HincII GTYRAC 2 cut(s) 523, 556
HindII GTYRAC 2 cut(s) 523, 556
HinfI GANTC 3 cut(s) 402, 613, 623
HpaII CCGG 2 cut(s) 314, 716
HphI GGTGA 1 cut(s) 739
Hpy166II GTNNAC 3 cut(s) 523, 556, 652
Hpy188I TCNGA 4 cut(s) 187, 285, 595, 612
Hpy188III TCNNGA 2 cut(s) 255, 617
Hpy8I GTNNAC 3 cut(s) 523, 556, 652
HpyAV CCTTC 1 cut(s) 617
HpyCH4III ACNGT 3 cut(s) 293, 574, 640
HpyCH4IV ACGT 2 cut(s) 90, 525
HpyCH4V TGCA 2 cut(s) 195, 296
HpyF10VI GCNNNNNNNGC 4 cut(s) 302, 323, 464, 467
HpyF3I CTNAG 4 cut(s) 169, 390, 471, 594
HpySE526I ACGT 2 cut(s) 90, 525
Hsp92II CATG 3 cut(s) 513, 553, 668
HspAI GCGC 1 cut(s) 230
LguI GCTCTTC 1 cut(s) 591
Lsp1109I GCAGC 3 cut(s) 221, 289, 454
LweI GCATC 3 cut(s) 163, 241, 454
MaeI CTAG 1 cut(s) 751
MaeII ACGT 2 cut(s) 90, 525
MaeIII GTNAC 2 cut(s) 337, 545
MboII GAAGA 4 cut(s) 127, 169, 578, 636
MlsI TGGCCA 1 cut(s) 569
MluCI AATT 3 cut(s) 250, 275, 601
MluNI TGGCCA 1 cut(s) 569
MmeI TCCRAC 2 cut(s) 210, 673
MnlI CCTC 8 cut(s) 39, 111, 142, 375, 416, 436, 625, 691
Mox20I TGGCCA 1 cut(s) 569
Mph1103I ATGCAT 1 cut(s) 197
MscI TGGCCA 1 cut(s) 569
MseI TTAA 2 cut(s) 153, 707
Msp20I TGGCCA 1 cut(s) 569
MspI CCGG 2 cut(s) 314, 716
MspR9I CCNGG 1 cut(s) 566
MvaI CCWGG 1 cut(s) 566
MwoI GCNNNNNNNGC 4 cut(s) 302, 323, 464, 467
NdeI CATATG 1 cut(s) 191
NlaIII CATG 3 cut(s) 513, 553, 668
NmuCI GTSAC 1 cut(s) 545
NsiI ATGCAT 1 cut(s) 197
PaqCI CACCTGC 1 cut(s) 491
PciSI GCTCTTC 1 cut(s) 591
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PfeI GAWTC 3 cut(s) 402, 613, 623
PflFI GACNNNGTC 1 cut(s) 551
PinAI ACCGGT 1 cut(s) 715
PkrI GCNGC 5 cut(s) 211, 304, 457, 460, 469
Psp1406I AACGTT 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 564
PspFI CCCAGC 1 cut(s) 226
PspGI CCWGG 1 cut(s) 564
PspPI GGNCC 1 cut(s) 8
PstNI CAGNNNCTG 2 cut(s) 302, 467
PsyI GACNNNGTC 1 cut(s) 551
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
SalI GTCGAC 1 cut(s) 554
SapI GCTCTTC 1 cut(s) 591
SaqAI TTAA 2 cut(s) 153, 707
SatI GCNGC 5 cut(s) 210, 303, 456, 459, 468
Sau96I GGNCC 1 cut(s) 8
ScrFI CCNGG 1 cut(s) 566
SfaNI GCATC 3 cut(s) 163, 241, 454
SmlI CTYRAG 1 cut(s) 534
SmoI CTYRAG 1 cut(s) 534
Sse9I AATT 3 cut(s) 250, 275, 601
SsiI CCGC 3 cut(s) 456, 459, 498
SspMI CTAG 1 cut(s) 751
StyD4I CCNGG 1 cut(s) 564
StyI CCWWGG 1 cut(s) 355
TaaI ACNGT 3 cut(s) 293, 574, 640
TaiI ACGT 2 cut(s) 93, 528
TaqI TCGA 1 cut(s) 555
TasI AATT 3 cut(s) 250, 275, 601
TauI GCSGC 2 cut(s) 458, 461
TfiI GAWTC 3 cut(s) 402, 613, 623
Tru1I TTAA 2 cut(s) 153, 707
Tru9I TTAA 2 cut(s) 153, 707
TscAI CASTG 4 cut(s) 423, 633, 645, 654
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 3 cut(s) 209, 302, 467
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 3 cut(s) 17, 498, 578
TspRI CASTG 4 cut(s) 423, 633, 645, 654
Tth111I GACNNNGTC 1 cut(s) 551
XapI RAATTY 2 cut(s) 250, 275
XmiI GTMKAC 1 cut(s) 555
XspI CTAG 1 cut(s) 751
Zsp2I ATGCAT 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.