MD15G1085400.v1.1

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003463
Physical Location & Seq
Forward (+)
5884807 .. 5886162
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1085400.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGCAAAATCATCTTTTCACTCTCCCACTAATGGAAGCCTCATCACTGTTCTTAGCATTGATGGAGGTGGAATAAGAGGGATTATTCCTGGAACTATACTCAGTTTTCTGGAGTCTGAGCTTCAGTTGAAAAAGAACAATAGCCTAGATGCTTTGCTATCGGATATATGTATGGGAACTTCAGCAGCTCCATACTACCTTCCCGCATATCAATTTAAATCGACTAATCCAATTGGTGAAACAAGAGAATTCAATCTCACTGATGGTGGCGTAGCTGCTAATAACCCGGCTTTGGTTGCTATGAGCGAAGTTACAAAACAAATCCACAAGGGTAGCCCTGACTTCGTTCCTTTGGGTGTAAATGAAAAGTTGTATGAGAGGTTCCTAGTTATATCACTGGGTACCGGTACAACATCTGAGGAGAAGTACGATGCCAAGGAAGCATCTGAGTGGGGTGCTTTGGGATGGTTGATTGGATCTAATTTTTCAGCTCCTCTAGTAGACATATTCAGTCAAGCGAGTAGTGACATGGTTGATTTCCACCTTGCTACCGTTTTTCAAGCTCGAGATGACACACTGAGTGGAACTCTGGCTTCCGTGGATATTGCTACTTCGGGAAACTTAAATGATCTCGTAAAAGTTGGGGAGAAATTGTTAACCAAACCAGTTTCAAGAGTTGACTTCAATACTGGAAAAGCAAAGCCTGTTCCTCCTGAAATTACAAATCAAGAGGCTCTCATAAGGGTGGCCGGAGTTCTCTCAAGGGAGAGGGCTGAAAGGAGCAAGTTCATGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

266

Amino Acids

28.42

Weight (kDa)

5.19

Isoelectric Point (pI)

26.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 45 - 101 8e-06 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 401
AccB1I GGYRCC 1 cut(s) 401
AccI GTMKAC 1 cut(s) 501
AciI CCGC 1 cut(s) 204
AclWI GGATC 1 cut(s) 484
AcoI YGGCCR 1 cut(s) 747
AcsI RAATTY 1 cut(s) 248
AcuI CTGAAG 2 cut(s) 107, 165
AdeI CACNNNGTG 1 cut(s) 581
AfaI GTAC 3 cut(s) 403, 409, 428
AfiI CCNNNNNNNGG 2 cut(s) 32, 292
AgeI ACCGGT 1 cut(s) 404
AgsI TTSAA 5 cut(s) 130, 253, 560, 672, 685
AjnI CCWGG 1 cut(s) 88
AluBI AGCT 5 cut(s) 121, 188, 275, 491, 563
AluI AGCT 5 cut(s) 121, 188, 275, 491, 563
AlwI GGATC 1 cut(s) 484
Ama87I CYCGRG 1 cut(s) 564
AoxI GGCC 1 cut(s) 747
ApeKI GCWGC 2 cut(s) 185, 275
ApoI RAATTY 1 cut(s) 248
AsiGI ACCGGT 1 cut(s) 404
Asp718I GGTACC 1 cut(s) 401
AsuC2I CCSGG 1 cut(s) 287
AsuHPI GGTGA 1 cut(s) 248
AvaI CYCGRG 1 cut(s) 564
BanI GGYRCC 1 cut(s) 401
BarI GAAGNNNNNNTAC 2 cut(s) 163, 195
BbvI GCAGC 2 cut(s) 197, 262
BccI CCATC 3 cut(s) 56, 257, 459
BciT130I CCWGG 1 cut(s) 90
BcnI CCSGG 1 cut(s) 287
BfaI CTAG 3 cut(s) 146, 386, 497
BisI GCNGC 2 cut(s) 186, 276
BlsI GCNGC 2 cut(s) 187, 277
Bme1390I CCNGG 2 cut(s) 90, 287
BmeT110I CYCGRG 1 cut(s) 564
BmiI GGNNCC 2 cut(s) 383, 403
BmrFI CCNGG 2 cut(s) 90, 287
BmrI ACTGGG 1 cut(s) 407
BmsI GCATC 3 cut(s) 139, 421, 452
BmuI ACTGGG 1 cut(s) 407
BplI GAGNNNNNCTC 2 cut(s) 571, 603
BpmI CTGGAG 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 745
BpuMI CCSGG 1 cut(s) 287
BsaJI CCNNGG 2 cut(s) 435, 597
BsaWI WCCGGW 1 cut(s) 404
BsaXI ACNNNNNCTCC 2 cut(s) 638, 668
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 292
Bse118I RCCGGY 1 cut(s) 404
Bse1I ACTGG 3 cut(s) 402, 665, 694
BseBI CCWGG 1 cut(s) 90
BseDI CCNNGG 2 cut(s) 435, 597
BseGI GGATG 1 cut(s) 470
BseLI CCNNNNNNNGG 2 cut(s) 32, 292
BseMII CTCAG 5 cut(s) 108, 115, 408, 438, 569
BseNI ACTGG 3 cut(s) 402, 665, 694
BseRI GAGGAG 2 cut(s) 434, 483
BseXI GCAGC 2 cut(s) 197, 262
BshFI GGCC 1 cut(s) 749
BshNI GGYRCC 1 cut(s) 401
BshTI ACCGGT 1 cut(s) 404
BsiHKCI CYCGRG 1 cut(s) 564
BsiSI CCGG 3 cut(s) 287, 405, 750
BslI CCNNNNNNNGG 2 cut(s) 32, 292
BsnI GGCC 1 cut(s) 749
BsoBI CYCGRG 1 cut(s) 564
Bsp143I GATC 2 cut(s) 476, 628
BspACI CCGC 1 cut(s) 204
BspANI GGCC 1 cut(s) 749
BspCNI CTCAG 5 cut(s) 109, 114, 409, 439, 570
BspHI TCATGA 1 cut(s) 789
BspLI GGNNCC 2 cut(s) 383, 403
BspPI GGATC 1 cut(s) 484
BspT107I GGYRCC 1 cut(s) 401
BsrFI RCCGGY 1 cut(s) 404
BsrI ACTGG 3 cut(s) 402, 665, 694
BssAI RCCGGY 1 cut(s) 404
BssECI CCNNGG 2 cut(s) 435, 597
BssMI GATC 2 cut(s) 476, 628
BssT1I CCWWGG 1 cut(s) 435
Bst2UI CCWGG 1 cut(s) 90
Bst4CI ACNGT 2 cut(s) 49, 553
BstDEI CTNAG 6 cut(s) 53, 101, 117, 417, 447, 578
BstDSI CCRYGG 1 cut(s) 597
BstF5I GGATG 1 cut(s) 470
BstKTI GATC 2 cut(s) 479, 631
BstMBI GATC 2 cut(s) 476, 628
BstMWI GCNNNNNNNGC 2 cut(s) 296, 440
BstNI CCWGG 1 cut(s) 90
BstSCI CCNGG 2 cut(s) 88, 285
BstV1I GCAGC 2 cut(s) 197, 262
BstX2I RGATCY 1 cut(s) 476
BstYI RGATCY 1 cut(s) 476
BsuRI GGCC 1 cut(s) 749
BtgI CCRYGG 1 cut(s) 597
BtsCI GGATG 1 cut(s) 470
BtsIMutI CAGTG 4 cut(s) 45, 258, 395, 575
CciI TCATGA 1 cut(s) 789
Cfr10I RCCGGY 1 cut(s) 404
Csp6I GTAC 3 cut(s) 402, 408, 427
CspAI ACCGGT 1 cut(s) 404
CviAII CATG 2 cut(s) 529, 790
CviQI GTAC 3 cut(s) 402, 408, 427
DdeI CTNAG 6 cut(s) 53, 101, 117, 417, 447, 578
DpnI GATC 2 cut(s) 478, 630
DpnII GATC 2 cut(s) 476, 628
DraI TTTAAA 1 cut(s) 217
DraIII CACNNNGTG 1 cut(s) 581
EaeI YGGCCR 1 cut(s) 747
Eco130I CCWWGG 1 cut(s) 435
Eco57I CTGAAG 2 cut(s) 107, 165
Eco88I CYCGRG 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 2 cut(s) 532, 793
FatI CATG 2 cut(s) 528, 789
FauI CCCGC 1 cut(s) 211
FblI GTMKAC 1 cut(s) 501
Fnu4HI GCNGC 2 cut(s) 186, 276
FokI GGATG 1 cut(s) 477
Fsp4HI GCNGC 2 cut(s) 186, 276
FspBI CTAG 3 cut(s) 146, 386, 497
GluI GCNGC 2 cut(s) 186, 276
GsuI CTGGAG 1 cut(s) 131
HaeIII GGCC 1 cut(s) 749
HapII CCGG 3 cut(s) 287, 405, 750
Hin1II CATG 2 cut(s) 532, 793
HincII GTYRAC 2 cut(s) 657, 679
HindII GTYRAC 2 cut(s) 657, 679
HinfI GANTC 1 cut(s) 113
HpaI GTTAAC 1 cut(s) 657
HpaII CCGG 3 cut(s) 287, 405, 750
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 3 cut(s) 502, 657, 679
Hpy188I TCNGA 4 cut(s) 118, 163, 418, 448
Hpy188III TCNNGA 7 cut(s) 110, 566, 615, 672, 713, 728, 790
Hpy8I GTNNAC 3 cut(s) 502, 657, 679
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 2 cut(s) 49, 553
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 440
HpyF3I CTNAG 6 cut(s) 53, 101, 117, 417, 447, 578
Hsp92II CATG 2 cut(s) 532, 793
KpnI GGTACC 1 cut(s) 405
KspAI GTTAAC 1 cut(s) 657
Kzo9I GATC 2 cut(s) 476, 628
LmnI GCTCC 3 cut(s) 193, 496, 780
Lsp1109I GCAGC 2 cut(s) 197, 262
LweI GCATC 3 cut(s) 139, 421, 452
MaeI CTAG 3 cut(s) 146, 386, 497
MaeIII GTNAC 2 cut(s) 310, 524
MalI GATC 2 cut(s) 478, 630
MboI GATC 2 cut(s) 476, 628
MfeI CAATTG 1 cut(s) 231
MflI RGATCY 1 cut(s) 476
MluCI AATT 6 cut(s) 212, 231, 248, 481, 650, 717
MlyI GAGTC 1 cut(s) 122
MnlI CCTC 9 cut(s) 50, 59, 71, 372, 412, 504, 720, 724, 762
MseI TTAA 3 cut(s) 216, 623, 656
MslI CAYNNNNRTG 2 cut(s) 448, 743
MspI CCGG 3 cut(s) 287, 405, 750
MspR9I CCNGG 2 cut(s) 90, 287
MunI CAATTG 1 cut(s) 231
MvaI CCWGG 1 cut(s) 90
MwoI GCNNNNNNNGC 2 cut(s) 296, 440
NciI CCSGG 1 cut(s) 287
NdeII GATC 2 cut(s) 476, 628
NlaIII CATG 2 cut(s) 532, 793
NlaIV GGNNCC 2 cut(s) 383, 403
NmuCI GTSAC 1 cut(s) 524
PaeR7I CTCGAG 1 cut(s) 564
PagI TCATGA 1 cut(s) 789
PfoI TCCNGGA 1 cut(s) 88
PinAI ACCGGT 1 cut(s) 404
PkrI GCNGC 2 cut(s) 187, 277
PleI GAGTC 1 cut(s) 121
PpsI GAGTC 1 cut(s) 121
Psp6I CCWGG 1 cut(s) 88
PspGI CCWGG 1 cut(s) 88
PspN4I GGNNCC 2 cut(s) 383, 403
PsrI GAACNNNNNNTAC 2 cut(s) 365, 397
PsuI RGATCY 1 cut(s) 476
RsaI GTAC 3 cut(s) 403, 409, 428
RsaNI GTAC 3 cut(s) 402, 408, 427
RseI CAYNNNNRTG 2 cut(s) 448, 743
SaqAI TTAA 3 cut(s) 216, 623, 656
SatI GCNGC 2 cut(s) 186, 276
Sau3AI GATC 2 cut(s) 476, 628
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 2 cut(s) 90, 287
SetI ASST 9 cut(s) 70, 123, 190, 201, 277, 383, 493, 546, 565
SfaNI GCATC 3 cut(s) 139, 421, 452
Sfr274I CTCGAG 1 cut(s) 564
SlaI CTCGAG 1 cut(s) 564
SmiI ATTTAAAT 1 cut(s) 217
SmiMI CAYNNNNRTG 2 cut(s) 448, 743
SmlI CTYRAG 2 cut(s) 564, 760
SmoI CTYRAG 2 cut(s) 564, 760
Sse9I AATT 6 cut(s) 212, 231, 248, 481, 650, 717
SsiI CCGC 1 cut(s) 204
SspMI CTAG 3 cut(s) 146, 386, 497
StyD4I CCNGG 2 cut(s) 88, 285
StyI CCWWGG 1 cut(s) 435
SwaI ATTTAAAT 1 cut(s) 217
TaaI ACNGT 2 cut(s) 49, 553
TaqI TCGA 2 cut(s) 221, 565
TasI AATT 6 cut(s) 212, 231, 248, 481, 650, 717
Tru1I TTAA 3 cut(s) 216, 623, 656
Tru9I TTAA 3 cut(s) 216, 623, 656
TscAI CASTG 4 cut(s) 52, 265, 402, 582
TseFI GTSAC 1 cut(s) 524
TseI GCWGC 2 cut(s) 185, 275
Tsp45I GTSAC 1 cut(s) 524
TspDTI ATGAA 2 cut(s) 378, 778
TspGWI ACGGA 1 cut(s) 586
TspRI CASTG 4 cut(s) 52, 265, 402, 582
XapI RAATTY 1 cut(s) 248
XhoI CTCGAG 1 cut(s) 564
XmiI GTMKAC 1 cut(s) 501
XspI CTAG 3 cut(s) 146, 386, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.