Rh6AG468800

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0003463
Physical Location & Seq
Reverse (-)
65129491 .. 65209265
79775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG468800.1

Sequence Viewer

Length: 1236 bp
ATGGAGAAAGCAGGTGCATTCCTTTGCCATCCCCCTCCCTCACATGGAAAGCTCATCACTGTTCTCAGCATTGATGGAGGTGGAATCAAAGGGATTATCCCCGGGACTCTACTCAGCTTCCTAGAGTCCGAGCTCCAGAAGCTGGATGGTGAGGCTGCAAGGTTGGCAGATTATTTCGATGTTATTGCAGGGACTAGCACAGGTGGCCTTGTCACTGCCATGCTTGCCGCCCCGGATCAAAACAACCGTCCCTTGTTTGCGGCTAACCAGATTGTCGATTTTTACCTTGAGCACTGCCCTAAAATATTCCCTCAAATCAATATTCCACTGTTTGGTGACGCCTTAGAAAATGCAAAGGGTGTATACGCTCCAAAATATGATGGCAAGTATTTACACAAACTTATCAAGGAAAAATTAGGAGATAGACGATTGAACCAGACATTGACCAATGTTGTGATCCCAACTTTTGATATAAGTTGCCTCCAACCAGCTATCTTCTCCACCTACGAGTTGAAAAGGAACAAGAAATTAGATCCGGCTCTACTGTCGGATATATGCATCGGAACTTCAGCAGCACCAACTTATCTTCCCTCGCATTATTTCAAAACCGCAACAAGAGAATTCCATCTCGTTGATGGCGGTGTGGCTGCTAATAACCCGGGTTTGACTGCTATAACCGAAGTGACAAAAGAAATCAAGAAGGGAAATACTGACTTCTCTCCGGTTGAGGCAGCTGATCAGGGTAATAGGTTCCTCCTCATATCTTTGGGTACTGGTTCATCAGCAAAATCCAAGTACGATGCCAGAGTAGCAGCTAAGTGGGGTATTATGGCATGGTTGATGCAAGCCAACTTTCAATGTCCATTGTTAGATGTACTCACTGAAGCAAGTAGTGACATGGTAGATTGCCACCTTTCTACCCTTTTCCAAGCTCTTTGGTCTGACGATAACTACCTTCGAATTCAGGATGACCAATTGAGCACCGACTTGAATTCTATGGATAATGCTACGCCCGAAAACTTGAAAAAACTTGTAAAAGTGGGAGAGGCACTGTTAAAGAAACAAGCTTCAAAGTTGAATATAGCTACCGGCCTCCATGAGCCTGATGAACGTCATATTACTAATGAAGAGGCTCTCAGAAGGTACTTACTGATTACAAATTACTCTCTGTACGTACTGGGATTTGTATCAAAGCATCATAAACATATATTCATTAATATTACTATTATTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

411

Amino Acids

45.25

Weight (kDa)

6.32

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 22 - 220 9.6e-22 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2
Acc36I ACCTGC 1 cut(s) 2
AccB7I CCANNNNNTGG 2 cut(s) 142, 332
AccI GTMKAC 1 cut(s) 363
AciI CCGC 4 cut(s) 228, 260, 609, 639
AclWI GGATC 3 cut(s) 243, 451, 527
AcsI RAATTY 3 cut(s) 620, 960, 991
AcuI CTGAAG 2 cut(s) 552, 903
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 6 cut(s) 772, 797, 876, 1145, 1172, 1176
AfiI CCNNNNNNNGG 3 cut(s) 44, 142, 332
AgsI TTSAA 8 cut(s) 433, 514, 604, 857, 991, 1024, 1071, 1078
Alw21I GWGCWC 3 cut(s) 135, 294, 983
AlwI GGATC 3 cut(s) 243, 451, 527
AlwNI CAGNNNCTG 1 cut(s) 142
Ama87I CYCGRG 2 cut(s) 101, 658
AoxI GGCC 2 cut(s) 205, 1090
ApeKI GCWGC 5 cut(s) 155, 572, 647, 731, 812
ApoI RAATTY 3 cut(s) 620, 960, 991
ArsI GACNNNNNNTTYG 2 cut(s) 232, 264
AseI ATTAAT 2 cut(s) 1215, 1230
AsuC2I CCSGG 5 cut(s) 102, 103, 233, 659, 660
AsuHPI GGTGA 2 cut(s) 161, 347
AsuII TTCGAA 1 cut(s) 958
AvaI CYCGRG 2 cut(s) 101, 658
BanII GRGCYC 1 cut(s) 135
Bbv12I GWGCWC 3 cut(s) 135, 294, 983
BbvI GCAGC 5 cut(s) 142, 584, 634, 743, 824
BccI CCATC 6 cut(s) 36, 68, 140, 374, 629, 633
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 5 cut(s) 102, 103, 233, 659, 660
BfaI CTAG 2 cut(s) 122, 195
BfuAI ACCTGC 1 cut(s) 2
BisI GCNGC 7 cut(s) 156, 228, 261, 573, 648, 732, 813
BlsI GCNGC 7 cut(s) 157, 229, 262, 574, 649, 733, 814
Bme1390I CCNGG 5 cut(s) 102, 103, 233, 659, 660
BmeT110I CYCGRG 2 cut(s) 101, 658
BmiI GGNNCC 1 cut(s) 752
BmrFI CCNGG 5 cut(s) 102, 103, 233, 659, 660
BmrI ACTGGG 1 cut(s) 1187
BmsI GCATC 4 cut(s) 567, 790, 831, 1204
BmuI ACTGGG 1 cut(s) 1187
BpmI CTGGAG 1 cut(s) 119
Bpu14I TTCGAA 1 cut(s) 958
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 5 cut(s) 102, 103, 233, 659, 660
BsaAI YACGTR 1 cut(s) 1174
BsaBI GATNNNNATC 1 cut(s) 1186
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 4 cut(s) 100, 101, 231, 658
BsaWI WCCGGW 1 cut(s) 721
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 142, 332
Bse118I RCCGGY 1 cut(s) 1088
Bse1I ACTGG 2 cut(s) 778, 1182
Bse8I GATNNNNATC 1 cut(s) 1186
BseDI CCNNGG 4 cut(s) 100, 101, 231, 658
BseGI GGATG 3 cut(s) 28, 151, 973
BseJI GATNNNNATC 1 cut(s) 1186
BseLI CCNNNNNNNGG 3 cut(s) 44, 142, 332
BseMII CTCAG 3 cut(s) 79, 127, 1150
BseNI ACTGG 2 cut(s) 778, 1182
BseRI GAGGAG 1 cut(s) 746
BseXI GCAGC 5 cut(s) 142, 584, 634, 743, 824
BshFI GGCC 2 cut(s) 207, 1092
BsiHKAI GWGCWC 3 cut(s) 135, 294, 983
BsiHKCI CYCGRG 2 cut(s) 101, 658
BsiSI CCGG 6 cut(s) 102, 233, 536, 659, 722, 1089
BslFI GGGAC 3 cut(s) 118, 205, 234
BslI CCNNNNNNNGG 3 cut(s) 44, 142, 332
BsmFI GGGAC 3 cut(s) 118, 205, 234
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 2 cut(s) 207, 1092
BsoBI CYCGRG 2 cut(s) 101, 658
Bsp119I TTCGAA 1 cut(s) 958
Bsp1286I GDGCHC 3 cut(s) 135, 294, 983
Bsp143I GATC 4 cut(s) 235, 456, 532, 736
BspACI CCGC 4 cut(s) 228, 260, 609, 639
BspANI GGCC 2 cut(s) 207, 1092
BspCNI CTCAG 3 cut(s) 78, 126, 1149
BspLI GGNNCC 1 cut(s) 752
BspMI ACCTGC 1 cut(s) 2
BspPI GGATC 3 cut(s) 243, 451, 527
BspT104I TTCGAA 1 cut(s) 958
BsrFI RCCGGY 1 cut(s) 1088
BsrI ACTGG 2 cut(s) 778, 1182
BssAI RCCGGY 1 cut(s) 1088
BssECI CCNNGG 4 cut(s) 100, 101, 231, 658
BssMI GATC 4 cut(s) 235, 456, 532, 736
BssNAI GTATAC 1 cut(s) 364
BssNI GRCGYC 1 cut(s) 339
Bst1107I GTATAC 1 cut(s) 364
Bst4CI ACNGT 5 cut(s) 61, 248, 330, 546, 1053
Bst6I CTCTTC 1 cut(s) 1122
BstACI GRCGYC 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 1174
BstBI TTCGAA 1 cut(s) 958
BstC8I GCNNGC 2 cut(s) 225, 846
BstDEI CTNAG 5 cut(s) 65, 113, 343, 816, 1136
BstF5I GGATG 3 cut(s) 28, 151, 973
BstKTI GATC 4 cut(s) 238, 459, 535, 739
BstMBI GATC 4 cut(s) 235, 456, 532, 736
BstMWI GCNNNNNNNGC 5 cut(s) 139, 164, 204, 224, 809
BstSCI CCNGG 5 cut(s) 100, 101, 231, 657, 658
BstSNI TACGTA 1 cut(s) 1174
BstV1I GCAGC 5 cut(s) 142, 584, 634, 743, 824
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
BstZ17I GTATAC 1 cut(s) 364
BsuRI GGCC 2 cut(s) 207, 1092
BtsCI GGATG 3 cut(s) 28, 151, 973
BtsI GCAGTG 2 cut(s) 213, 292
BtsIMutI CAGTG 6 cut(s) 57, 213, 292, 326, 879, 1049
BveI ACCTGC 1 cut(s) 2
Cac8I GCNNGC 2 cut(s) 225, 846
CaiI CAGNNNCTG 1 cut(s) 142
Cfr10I RCCGGY 1 cut(s) 1088
Cfr9I CCCGGG 2 cut(s) 101, 658
CseI GACGC 1 cut(s) 347
Csp6I GTAC 6 cut(s) 771, 796, 875, 1144, 1171, 1175
CviAII CATG 5 cut(s) 44, 220, 834, 898, 1097
CviQI GTAC 6 cut(s) 771, 796, 875, 1144, 1171, 1175
DdeI CTNAG 5 cut(s) 65, 113, 343, 816, 1136
DpnI GATC 4 cut(s) 237, 458, 534, 738
DpnII GATC 4 cut(s) 235, 456, 532, 736
Eam1104I CTCTTC 1 cut(s) 1122
EarI CTCTTC 1 cut(s) 1122
Ecl136II GAGCTC 1 cut(s) 133
Eco105I TACGTA 1 cut(s) 1174
Eco24I GRGCYC 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 2 cut(s) 552, 903
Eco88I CYCGRG 2 cut(s) 101, 658
EcoICRI GAGCTC 1 cut(s) 133
EcoRI GAATTC 3 cut(s) 620, 960, 991
EcoT22I ATGCAT 1 cut(s) 560
EcoT38I GRGCYC 1 cut(s) 135
FaeI CATG 5 cut(s) 47, 223, 837, 901, 1100
FaqI GGGAC 3 cut(s) 118, 205, 234
FatI CATG 5 cut(s) 43, 219, 833, 897, 1096
FbaI TGATCA 1 cut(s) 736
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 7 cut(s) 156, 228, 261, 573, 648, 732, 813
FokI GGATG 3 cut(s) 15, 158, 980
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 7 cut(s) 156, 228, 261, 573, 648, 732, 813
FspBI CTAG 2 cut(s) 122, 195
GluI GCNGC 7 cut(s) 156, 228, 261, 573, 648, 732, 813
GsuI CTGGAG 1 cut(s) 119
HaeIII GGCC 2 cut(s) 207, 1092
HapII CCGG 6 cut(s) 102, 233, 536, 659, 722, 1089
HgaI GACGC 1 cut(s) 347
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 5 cut(s) 47, 223, 837, 901, 1100
HindIII AAGCTT 1 cut(s) 1065
HinfI GANTC 3 cut(s) 84, 106, 125
HpaII CCGG 6 cut(s) 102, 233, 536, 659, 722, 1089
HphI GGTGA 2 cut(s) 161, 347
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 5 cut(s) 130, 550, 563, 943, 1139
Hpy188III TCNNGA 3 cut(s) 136, 697, 965
Hpy8I GTNNAC 1 cut(s) 364
HpyAV CCTTC 3 cut(s) 694, 965, 1134
HpyCH4III ACNGT 5 cut(s) 61, 248, 330, 546, 1053
HpyCH4IV ACGT 2 cut(s) 1111, 1173
HpyCH4V TGCA 6 cut(s) 17, 158, 188, 353, 558, 844
HpyF10VI GCNNNNNNNGC 5 cut(s) 139, 164, 204, 224, 809
HpyF3I CTNAG 5 cut(s) 65, 113, 343, 816, 1136
HpySE526I ACGT 2 cut(s) 1111, 1173
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 5 cut(s) 47, 223, 837, 901, 1100
Ksp22I TGATCA 1 cut(s) 736
Kzo9I GATC 4 cut(s) 235, 456, 532, 736
LmnI GCTCC 2 cut(s) 138, 373
Lsp1109I GCAGC 5 cut(s) 142, 584, 634, 743, 824
LweI GCATC 4 cut(s) 567, 790, 831, 1204
MaeI CTAG 2 cut(s) 122, 195
MaeII ACGT 2 cut(s) 1111, 1173
MaeIII GTNAC 4 cut(s) 211, 335, 682, 893
MalI GATC 4 cut(s) 237, 458, 534, 738
MboI GATC 4 cut(s) 235, 456, 532, 736
MboII GAAGA 3 cut(s) 487, 578, 1139
MfeI CAATTG 1 cut(s) 974
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 3 cut(s) 135, 294, 983
MluCI AATT 8 cut(s) 413, 527, 620, 960, 974, 991, 1159, 1231
MlyI GAGTC 2 cut(s) 100, 134
MmeI TCCRAC 2 cut(s) 508, 528
Mph1103I ATGCAT 1 cut(s) 560
MseI TTAA 3 cut(s) 1055, 1215, 1230
MslI CAYNNNNRTG 1 cut(s) 218
MspA1I CMGCKG 1 cut(s) 734
MspI CCGG 6 cut(s) 102, 233, 536, 659, 722, 1089
MspR9I CCNGG 5 cut(s) 102, 103, 233, 659, 660
MunI CAATTG 1 cut(s) 974
Mva1269I GAATGC 1 cut(s) 17
MwoI GCNNNNNNNGC 5 cut(s) 139, 164, 204, 224, 809
NciI CCSGG 5 cut(s) 102, 103, 233, 659, 660
NdeII GATC 4 cut(s) 235, 456, 532, 736
NlaIII CATG 5 cut(s) 47, 223, 837, 901, 1100
NlaIV GGNNCC 1 cut(s) 752
NmuCI GTSAC 4 cut(s) 211, 335, 682, 893
NsiI ATGCAT 1 cut(s) 560
NspV TTCGAA 1 cut(s) 958
PaqCI CACCTGC 1 cut(s) 2
PctI GAATGC 1 cut(s) 17
PfeI GAWTC 1 cut(s) 84
PflMI CCANNNNNTGG 2 cut(s) 142, 332
PkrI GCNGC 7 cut(s) 157, 229, 262, 574, 649, 733, 814
PleI GAGTC 2 cut(s) 100, 133
PpsI GAGTC 2 cut(s) 100, 133
Ppu21I YACGTR 1 cut(s) 1174
PshBI ATTAAT 2 cut(s) 1215, 1230
Psp124BI GAGCTC 1 cut(s) 135
PspN4I GGNNCC 1 cut(s) 752
PstNI CAGNNNCTG 1 cut(s) 142
PsuI RGATCY 1 cut(s) 532
PvuII CAGCTG 1 cut(s) 734
RsaI GTAC 6 cut(s) 772, 797, 876, 1145, 1172, 1176
RsaNI GTAC 6 cut(s) 771, 796, 875, 1144, 1171, 1175
RseI CAYNNNNRTG 1 cut(s) 218
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 3 cut(s) 1055, 1215, 1230
SatI GCNGC 7 cut(s) 156, 228, 261, 573, 648, 732, 813
Sau3AI GATC 4 cut(s) 235, 456, 532, 736
SchI GAGTC 2 cut(s) 100, 134
ScrFI CCNGG 5 cut(s) 102, 103, 233, 659, 660
SduI GDGCHC 3 cut(s) 135, 294, 983
SfaNI GCATC 4 cut(s) 567, 790, 831, 1204
SfuI TTCGAA 1 cut(s) 958
SmaI CCCGGG 2 cut(s) 103, 660
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SnaBI TACGTA 1 cut(s) 1174
Sse9I AATT 8 cut(s) 413, 527, 620, 960, 974, 991, 1159, 1231
SsiI CCGC 4 cut(s) 228, 260, 609, 639
SspI AATATT 3 cut(s) 306, 322, 1219
SspMI CTAG 2 cut(s) 122, 195
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 5 cut(s) 100, 101, 231, 657, 658
TaaI ACNGT 5 cut(s) 61, 248, 330, 546, 1053
TaiI ACGT 2 cut(s) 1114, 1176
TaqI TCGA 3 cut(s) 177, 276, 958
TasI AATT 8 cut(s) 413, 527, 620, 960, 974, 991, 1159, 1231
TatI WGTACW 1 cut(s) 874
TauI GCSGC 2 cut(s) 230, 263
TfiI GAWTC 1 cut(s) 84
Tru1I TTAA 3 cut(s) 1055, 1215, 1230
Tru9I TTAA 3 cut(s) 1055, 1215, 1230
TscAI CASTG 6 cut(s) 64, 220, 299, 333, 886, 1056
TseFI GTSAC 4 cut(s) 211, 335, 682, 893
TseI GCWGC 5 cut(s) 155, 572, 647, 731, 812
Tsp45I GTSAC 4 cut(s) 211, 335, 682, 893
TspDTI ATGAA 4 cut(s) 768, 1122, 1140, 1201
TspMI CCCGGG 2 cut(s) 101, 658
TspRI CASTG 6 cut(s) 64, 220, 299, 333, 886, 1056
Van91I CCANNNNNTGG 2 cut(s) 142, 332
VspI ATTAAT 2 cut(s) 1215, 1230
XapI RAATTY 3 cut(s) 620, 960, 991
XcmI CCANNNNNNNNNTGG 2 cut(s) 143, 632
XmaI CCCGGG 2 cut(s) 101, 658
XmiI GTMKAC 1 cut(s) 363
XspI CTAG 2 cut(s) 122, 195
Zsp2I ATGCAT 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.