Rroxscaffold_7G00160970

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003463
Physical Location & Seq
Forward (+)
3841974 .. 3844162
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00160970.1

Sequence Viewer

Length: 1227 bp
ATGGAGAAAGCAAGTGCATTCCTTTGCCATCCCCCTCCCTCACAGGGAAAGTTCATCACTGTTCTCAGCATTGATGGAGGTGGAATCAAAGGAATTATACCTGGGACTCTGCTCAGCTTCCTAGAGTCCGAGCTCCAGAAGCTGGATGGTGAAGCTGCAAGGTTGGCAGATTATTTCGATGTTATTGCAGGGACTAGCACAGGTGGCCTTGTCACCGCCATGCTTACCGCCCCGGATCAAAACAACCGTCCCTTGTTTGCGGCTAACCAGATTGTCGATTTTTACCTTGAGCACTGCCCTAAAATATTCCCTCAAATCAATATTCCACTGTTTGGTGACGCCTTAGAAAATGCAAAGGGTGTATACGCACCAAAATATGATGGCAAGTATTTACACAAGCTTATCAAGGAAAAATTAGGAGACAGACGATTGAACCAGACATTGACCAATGTTGTGATCCCAACTTTTGATATAAGTTGCCTCCAACCAGCTATCTTCTCCACCTACGAGTTGAAAAGGAACAAGAAATTAGATCCGGCTCTACTGTCGGATATATGCATCGGAACTTCAGCAGCACCAACTTATCTTCCCTCGCATTATTTCAAAACCGCCACAAGAGAATTCCATCTCGTTGATGGCGGTGTGGCTGCTAATAATCCGGGTTTGACTGCTATAACCGAAGTGACAAAAGAAATCAAGAAGGGAAATACTGACTTCTCTCCGGTTGAGGCAGCTGATCAGGGTAATAGGTTCCTCCTCATATCTTTGGGTACTGGTTCATCAGCAAAATCCAAGTACGATGCCAGAGTAGCAGCTAAGTGGGGTATTATGGCATGGTTGATGCAAGCCAACTTTCAATGTCCATTGTTAGATGTTCTCACTGAAGCAAGTAGTGACATGGTAGATTGCCACCTTTCTACCCTTTTCCAAGCTCTTTGGTCTGACGATAACTACCTTCGAATTCAGGATGACCAATTGAGTACCGACTTGAATTCTATGGATAATGCTTCGCCCGAAAACTTGAAAAAACTTGTAAAAGTGGGAGAGGCACTGTTAAAGAAACAAGCTTCAAAGTTGAATATAGCTACCGGCCTCCATGAGCCTGATGAACGTCATATTACTAATGAAGAGGCTCTCAGAAGGGTAGCGGCAGTACTCTCCAAAGAGAAGAAGGCACGTGCAATCAGATCAGCAGCGCCACAAGCAAACCCAGCATCTGCCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

408

Amino Acids

44.43

Weight (kDa)

6.47

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 22 - 220 4.6e-21 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 142, 332
AccI GTMKAC 1 cut(s) 363
AciI CCGC 6 cut(s) 216, 228, 260, 609, 639, 1148
AclWI GGATC 3 cut(s) 243, 451, 527
AcsI RAATTY 3 cut(s) 620, 960, 991
AcuI CTGAAG 2 cut(s) 552, 903
AcvI CACGTG 1 cut(s) 1178
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 4 cut(s) 772, 797, 982, 1155
AfiI CCNNNNNNNGG 3 cut(s) 44, 142, 332
AgsI TTSAA 8 cut(s) 433, 514, 604, 857, 991, 1024, 1071, 1078
AjnI CCWGG 1 cut(s) 100
Alw21I GWGCWC 2 cut(s) 135, 294
Alw26I GTCTC 1 cut(s) 414
AlwI GGATC 3 cut(s) 243, 451, 527
AlwNI CAGNNNCTG 2 cut(s) 142, 1217
AoxI GGCC 2 cut(s) 205, 1090
ApeKI GCWGC 6 cut(s) 155, 572, 647, 731, 812, 1193
ApoI RAATTY 3 cut(s) 620, 960, 991
ArsI GACNNNNNNTTYG 2 cut(s) 232, 264
AspLEI GCGC 1 cut(s) 1198
AsuC2I CCSGG 2 cut(s) 233, 660
AsuHPI GGTGA 3 cut(s) 161, 205, 347
AsuII TTCGAA 1 cut(s) 958
BanII GRGCYC 1 cut(s) 135
BbrPI CACGTG 1 cut(s) 1178
Bbv12I GWGCWC 2 cut(s) 135, 294
BbvI GCAGC 6 cut(s) 142, 584, 634, 743, 824, 1205
BccI CCATC 6 cut(s) 36, 68, 140, 374, 629, 633
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BciT130I CCWGG 1 cut(s) 102
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 2 cut(s) 233, 660
BcoDI GTCTC 1 cut(s) 414
BfaI CTAG 2 cut(s) 122, 195
BfoI RGCGCY 1 cut(s) 1199
BisI GCNGC 8 cut(s) 156, 261, 573, 648, 732, 813, 1149, 1194
BlpI GCTNAGC 1 cut(s) 113
BlsI GCNGC 8 cut(s) 157, 262, 574, 649, 733, 814, 1150, 1195
BmcAI AGTACT 1 cut(s) 1155
Bme1390I CCNGG 3 cut(s) 102, 233, 660
BmiI GGNNCC 1 cut(s) 752
BmrFI CCNGG 3 cut(s) 102, 233, 660
BmsI GCATC 4 cut(s) 567, 790, 831, 1223
BpmI CTGGAG 1 cut(s) 119
Bpu1102I GCTNAGC 1 cut(s) 113
Bpu14I TTCGAA 1 cut(s) 958
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 2 cut(s) 233, 660
BsaAI YACGTR 1 cut(s) 1178
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 2 cut(s) 101, 231
BsaWI WCCGGW 1 cut(s) 721
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 142, 332
Bse118I RCCGGY 1 cut(s) 1088
Bse1I ACTGG 1 cut(s) 778
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 101, 231
BseGI GGATG 3 cut(s) 28, 151, 973
BseLI CCNNNNNNNGG 3 cut(s) 44, 142, 332
BseMII CTCAG 3 cut(s) 79, 127, 1150
BseNI ACTGG 1 cut(s) 778
BseRI GAGGAG 1 cut(s) 746
BseXI GCAGC 6 cut(s) 142, 584, 634, 743, 824, 1205
BseYI CCCAGC 1 cut(s) 1210
BshFI GGCC 2 cut(s) 207, 1092
BsiHKAI GWGCWC 2 cut(s) 135, 294
BsiSI CCGG 5 cut(s) 233, 536, 659, 722, 1089
BslFI GGGAC 3 cut(s) 118, 205, 234
BslI CCNNNNNNNGG 3 cut(s) 44, 142, 332
BsmAI GTCTC 1 cut(s) 414
BsmFI GGGAC 3 cut(s) 118, 205, 234
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 2 cut(s) 207, 1092
Bsp119I TTCGAA 1 cut(s) 958
Bsp1286I GDGCHC 2 cut(s) 135, 294
Bsp143I GATC 5 cut(s) 235, 456, 532, 736, 1187
Bsp1720I GCTNAGC 1 cut(s) 113
BspACI CCGC 6 cut(s) 216, 228, 260, 609, 639, 1148
BspANI GGCC 2 cut(s) 207, 1092
BspCNI CTCAG 3 cut(s) 78, 126, 1149
BspLI GGNNCC 1 cut(s) 752
BspPI GGATC 3 cut(s) 243, 451, 527
BspT104I TTCGAA 1 cut(s) 958
BsrFI RCCGGY 1 cut(s) 1088
BsrI ACTGG 1 cut(s) 778
BssAI RCCGGY 1 cut(s) 1088
BssECI CCNNGG 2 cut(s) 101, 231
BssMI GATC 5 cut(s) 235, 456, 532, 736, 1187
BssNAI GTATAC 1 cut(s) 364
BssNI GRCGYC 1 cut(s) 339
Bst1107I GTATAC 1 cut(s) 364
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 5 cut(s) 61, 248, 330, 546, 1053
Bst6I CTCTTC 1 cut(s) 1122
BstACI GRCGYC 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 1178
BstBI TTCGAA 1 cut(s) 958
BstC8I GCNNGC 2 cut(s) 846, 1222
BstDEI CTNAG 5 cut(s) 65, 113, 343, 816, 1136
BstF5I GGATG 3 cut(s) 28, 151, 973
BstH2I RGCGCY 1 cut(s) 1199
BstHHI GCGC 1 cut(s) 1198
BstKTI GATC 5 cut(s) 238, 459, 535, 739, 1190
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 5 cut(s) 235, 456, 532, 736, 1187
BstMWI GCNNNNNNNGC 6 cut(s) 139, 164, 204, 809, 1202, 1211
BstNI CCWGG 1 cut(s) 102
BstSCI CCNGG 3 cut(s) 100, 231, 658
BstV1I GCAGC 6 cut(s) 142, 584, 634, 743, 824, 1205
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
BstZ17I GTATAC 1 cut(s) 364
BsuRI GGCC 2 cut(s) 207, 1092
BtsCI GGATG 3 cut(s) 28, 151, 973
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 5 cut(s) 57, 292, 326, 879, 1049
Cac8I GCNNGC 2 cut(s) 846, 1222
CaiI CAGNNNCTG 2 cut(s) 142, 1217
CfoI GCGC 1 cut(s) 1198
Cfr10I RCCGGY 1 cut(s) 1088
CseI GACGC 1 cut(s) 347
Csp6I GTAC 4 cut(s) 771, 796, 981, 1154
CviAII CATG 4 cut(s) 220, 834, 898, 1097
CviQI GTAC 4 cut(s) 771, 796, 981, 1154
DdeI CTNAG 5 cut(s) 65, 113, 343, 816, 1136
DpnI GATC 5 cut(s) 237, 458, 534, 738, 1189
DpnII GATC 5 cut(s) 235, 456, 532, 736, 1187
Eam1104I CTCTTC 1 cut(s) 1122
EarI CTCTTC 1 cut(s) 1122
Ecl136II GAGCTC 1 cut(s) 133
Eco24I GRGCYC 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 2 cut(s) 552, 903
Eco72I CACGTG 1 cut(s) 1178
EcoICRI GAGCTC 1 cut(s) 133
EcoRI GAATTC 3 cut(s) 620, 960, 991
EcoRII CCWGG 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 560
EcoT38I GRGCYC 1 cut(s) 135
FaeI CATG 4 cut(s) 223, 837, 901, 1100
FaqI GGGAC 3 cut(s) 118, 205, 234
FatI CATG 4 cut(s) 219, 833, 897, 1096
FbaI TGATCA 1 cut(s) 736
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 8 cut(s) 156, 261, 573, 648, 732, 813, 1149, 1194
FokI GGATG 3 cut(s) 15, 158, 980
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 8 cut(s) 156, 261, 573, 648, 732, 813, 1149, 1194
FspBI CTAG 2 cut(s) 122, 195
GlaI GCGC 1 cut(s) 1197
GluI GCNGC 8 cut(s) 156, 261, 573, 648, 732, 813, 1149, 1194
GsaI CCCAGC 1 cut(s) 1214
GsuI CTGGAG 1 cut(s) 119
HaeII RGCGCY 1 cut(s) 1199
HaeIII GGCC 2 cut(s) 207, 1092
HapII CCGG 5 cut(s) 233, 536, 659, 722, 1089
HgaI GACGC 1 cut(s) 347
HhaI GCGC 1 cut(s) 1198
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 4 cut(s) 223, 837, 901, 1100
Hin6I GCGC 1 cut(s) 1196
HinP1I GCGC 1 cut(s) 1196
HindIII AAGCTT 2 cut(s) 398, 1065
HinfI GANTC 3 cut(s) 84, 106, 125
HpaII CCGG 5 cut(s) 233, 536, 659, 722, 1089
HphI GGTGA 3 cut(s) 161, 205, 347
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 6 cut(s) 130, 550, 563, 943, 1139, 1187
Hpy188III TCNNGA 3 cut(s) 136, 697, 965
Hpy8I GTNNAC 1 cut(s) 364
HpyAV CCTTC 4 cut(s) 694, 965, 1134, 1165
HpyCH4III ACNGT 5 cut(s) 61, 248, 330, 546, 1053
HpyCH4IV ACGT 2 cut(s) 1111, 1177
HpyCH4V TGCA 7 cut(s) 17, 158, 188, 353, 558, 844, 1181
HpyF10VI GCNNNNNNNGC 6 cut(s) 139, 164, 204, 809, 1202, 1211
HpyF3I CTNAG 5 cut(s) 65, 113, 343, 816, 1136
HpySE526I ACGT 2 cut(s) 1111, 1177
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 4 cut(s) 223, 837, 901, 1100
HspAI GCGC 1 cut(s) 1196
Ksp22I TGATCA 1 cut(s) 736
Kzo9I GATC 5 cut(s) 235, 456, 532, 736, 1187
LmnI GCTCC 1 cut(s) 138
Lsp1109I GCAGC 6 cut(s) 142, 584, 634, 743, 824, 1205
LweI GCATC 4 cut(s) 567, 790, 831, 1223
MaeI CTAG 2 cut(s) 122, 195
MaeII ACGT 2 cut(s) 1111, 1177
MaeIII GTNAC 4 cut(s) 211, 335, 682, 893
MalI GATC 5 cut(s) 237, 458, 534, 738, 1189
MboI GATC 5 cut(s) 235, 456, 532, 736, 1187
MboII GAAGA 4 cut(s) 487, 578, 1139, 1180
MfeI CAATTG 1 cut(s) 974
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 2 cut(s) 135, 294
MluCI AATT 7 cut(s) 93, 413, 527, 620, 960, 974, 991
MlyI GAGTC 2 cut(s) 100, 134
MmeI TCCRAC 2 cut(s) 508, 528
Mph1103I ATGCAT 1 cut(s) 560
MseI TTAA 1 cut(s) 1055
MslI CAYNNNNRTG 1 cut(s) 218
MspA1I CMGCKG 2 cut(s) 734, 1224
MspI CCGG 5 cut(s) 233, 536, 659, 722, 1089
MspR9I CCNGG 3 cut(s) 102, 233, 660
MunI CAATTG 1 cut(s) 974
Mva1269I GAATGC 1 cut(s) 17
MvaI CCWGG 1 cut(s) 102
MwoI GCNNNNNNNGC 6 cut(s) 139, 164, 204, 809, 1202, 1211
NciI CCSGG 2 cut(s) 233, 660
NdeII GATC 5 cut(s) 235, 456, 532, 736, 1187
NlaIII CATG 4 cut(s) 223, 837, 901, 1100
NlaIV GGNNCC 1 cut(s) 752
NmuCI GTSAC 4 cut(s) 211, 335, 682, 893
NsiI ATGCAT 1 cut(s) 560
NspV TTCGAA 1 cut(s) 958
PctI GAATGC 1 cut(s) 17
PfeI GAWTC 1 cut(s) 84
PflMI CCANNNNNTGG 2 cut(s) 142, 332
PkrI GCNGC 8 cut(s) 157, 262, 574, 649, 733, 814, 1150, 1195
PleI GAGTC 2 cut(s) 100, 133
PmaCI CACGTG 1 cut(s) 1178
PmlI CACGTG 1 cut(s) 1178
PpsI GAGTC 2 cut(s) 100, 133
Ppu21I YACGTR 1 cut(s) 1178
Psp124BI GAGCTC 1 cut(s) 135
Psp6I CCWGG 1 cut(s) 100
PspCI CACGTG 1 cut(s) 1178
PspFI CCCAGC 1 cut(s) 1210
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 752
PstNI CAGNNNCTG 2 cut(s) 142, 1217
PsuI RGATCY 1 cut(s) 532
PvuII CAGCTG 2 cut(s) 734, 1224
RsaI GTAC 4 cut(s) 772, 797, 982, 1155
RsaNI GTAC 4 cut(s) 771, 796, 981, 1154
RseI CAYNNNNRTG 1 cut(s) 218
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 1 cut(s) 1055
SatI GCNGC 8 cut(s) 156, 261, 573, 648, 732, 813, 1149, 1194
Sau3AI GATC 5 cut(s) 235, 456, 532, 736, 1187
ScaI AGTACT 1 cut(s) 1155
SchI GAGTC 2 cut(s) 100, 134
ScrFI CCNGG 3 cut(s) 102, 233, 660
SduI GDGCHC 2 cut(s) 135, 294
SfaNI GCATC 4 cut(s) 567, 790, 831, 1223
SfuI TTCGAA 1 cut(s) 958
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 7 cut(s) 93, 413, 527, 620, 960, 974, 991
SsiI CCGC 6 cut(s) 216, 228, 260, 609, 639, 1148
SspI AATATT 2 cut(s) 306, 322
SspMI CTAG 2 cut(s) 122, 195
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 3 cut(s) 100, 231, 658
TaaI ACNGT 5 cut(s) 61, 248, 330, 546, 1053
TaiI ACGT 2 cut(s) 1114, 1180
TaqI TCGA 3 cut(s) 177, 276, 958
TasI AATT 7 cut(s) 93, 413, 527, 620, 960, 974, 991
TatI WGTACW 1 cut(s) 1153
TauI GCSGC 2 cut(s) 263, 1151
TfiI GAWTC 1 cut(s) 84
Tru1I TTAA 1 cut(s) 1055
Tru9I TTAA 1 cut(s) 1055
TscAI CASTG 5 cut(s) 64, 299, 333, 886, 1056
TseFI GTSAC 4 cut(s) 211, 335, 682, 893
TseI GCWGC 6 cut(s) 155, 572, 647, 731, 812, 1193
Tsp45I GTSAC 4 cut(s) 211, 335, 682, 893
TspDTI ATGAA 4 cut(s) 43, 768, 1122, 1140
TspRI CASTG 5 cut(s) 64, 299, 333, 886, 1056
Van91I CCANNNNNTGG 2 cut(s) 142, 332
XapI RAATTY 3 cut(s) 620, 960, 991
XcmI CCANNNNNNNNNTGG 2 cut(s) 143, 632
XmiI GTMKAC 1 cut(s) 363
XspI CTAG 2 cut(s) 122, 195
ZrmI AGTACT 1 cut(s) 1155
Zsp2I ATGCAT 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.