FvH4_4g24290

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003463
Physical Location & Seq
Reverse (-)
26523773 .. 26526092
2320 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24290.t1

Sequence Viewer

Length: 984 bp
ATGTTAAAGCTAGCTAGTCCAGATGGAGAAAGCAAGTGCAATCCTTTCCCACAACCCCCTCCCAGCCATGGAAAGCTAATCACAGTTCTCAGCATTGACGGAGGTGGAATCAAAGGGATTATTCCCGGAACTCTGCTCAGCTTCCTAGACTCCGAGCTCCAGAAGCTGGATGGTGAAAATGCAAGGTTGGCCGATTACTTTGATGTTATCGCCGGGACTAGCACGGGTGGCCTTGTCACCGCCATGCTTACTGCCCCAAATGAAAACAAACGTCCCCTGTTTGCGGCTAGCCAGATTGTCGATTTTCACCTTGAACACTGCCCTAAAATCTTCCCTCAAATCAAAGAATACCATCTCGTTGATGGCGGAGTGGCTGCTAATAACCCGGCTTTGACTGCTATAACTGAAGTGACAAAAGAAATCAAGAACGGAAATTCGGATTTCTCTCGGATTGTGCCAGCTGAGCAGGGAGACAGGTTCCTACTTGTATCATTGGGTACTGGTTCATCAGAAAAATCTCATTATGATGCCGATCAAGCAAAATTCTGGGGAATTTTGGGATGGTTGATGCAACCCAACTTCAAATGTCCATTGTTGTATGTACTTGGTGAAGCCAGTAGTGACATGGATGACAAACTGGATGCCAAACTGAATTGTATGGATAATGCCACGAAAGAAAACTTGAGAGACCTTAAGAAAGCGGGAGAGGCGCTGTTAAAAAAACGAGCTACAAAGCTGAATTTAGATATCGGTATCCATGAGCCTGATGAACGTAATATTCAGGGGCGGGGCCAGCCCAGCCCCAAATCCTTTCATCTTTTGCCCACTGGTTGTTGCTCTTGCCTGTCGCTCTTGCCTCTCATCGGTCGCTCTCGCCGCCGCGCTCCTGTCGATTCCCTTCCCAGTCCGGCAGTCCCCGAATCCCCCGATCCCTACTTCTTGACTTCTTGTCTTCCTCAATCCTCAGGTATGGCTATTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

328

Amino Acids

35.27

Weight (kDa)

6.0

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 30 - 103 8.6e-10 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 166
AccII CGCG 1 cut(s) 882
AciI CCGC 7 cut(s) 240, 284, 366, 701, 787, 877, 880
AclWI GGATC 1 cut(s) 923
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 4 cut(s) 433, 542, 552, 739
AcuI CTGAAG 1 cut(s) 426
AfaI GTAC 2 cut(s) 499, 603
AfiI CCNNNNNNNGG 4 cut(s) 68, 166, 283, 863
AflII CTTAAG 1 cut(s) 692
AgsI TTSAA 2 cut(s) 314, 583
AhdI GACNNNNNGTC 1 cut(s) 948
AluBI AGCT 9 cut(s) 10, 14, 76, 141, 157, 166, 461, 728, 736
AluI AGCT 9 cut(s) 10, 14, 76, 141, 157, 166, 461, 728, 736
Alw21I GWGCWC 1 cut(s) 159
Alw26I GTCTC 2 cut(s) 465, 681
AlwI GGATC 1 cut(s) 923
AlwNI CAGNNNCTG 1 cut(s) 166
AoxI GGCC 3 cut(s) 189, 229, 790
ApeKI GCWGC 1 cut(s) 374
ApoI RAATTY 4 cut(s) 433, 542, 552, 739
AspLEI GCGC 2 cut(s) 712, 884
AspS9I GGNCC 1 cut(s) 790
AsuC2I CCSGG 3 cut(s) 126, 214, 386
AsuHPI GGTGA 4 cut(s) 185, 229, 299, 620
AsuNHI GCTAGC 2 cut(s) 10, 287
AxyI CCTNAGG 1 cut(s) 964
BanII GRGCYC 1 cut(s) 159
BbsI GAAGAC 1 cut(s) 944
Bbv12I GWGCWC 1 cut(s) 159
BbvI GCAGC 1 cut(s) 361
BccI CCATC 5 cut(s) 17, 164, 356, 360, 555
BciVI GTATCC 1 cut(s) 764
BcnI CCSGG 3 cut(s) 126, 214, 386
BcoDI GTCTC 2 cut(s) 465, 681
BfaI CTAG 5 cut(s) 11, 15, 146, 219, 288
BfoI RGCGCY 1 cut(s) 713
BfrI CTTAAG 1 cut(s) 692
BfuI GTATCC 1 cut(s) 764
BisI GCNGC 4 cut(s) 285, 375, 877, 880
BlpI GCTNAGC 2 cut(s) 137, 462
BlsI GCNGC 4 cut(s) 286, 376, 878, 881
Bme1390I CCNGG 3 cut(s) 126, 214, 386
BmeRI GACNNNNNGTC 1 cut(s) 948
BmgT120I GGNCC 1 cut(s) 790
BmiI GGNNCC 2 cut(s) 479, 791
BmrFI CCNGG 3 cut(s) 126, 214, 386
BmrI ACTGGG 1 cut(s) 897
BmsI GCATC 3 cut(s) 517, 558, 631
BmtI GCTAGC 2 cut(s) 14, 291
BmuI ACTGGG 1 cut(s) 897
BpiI GAAGAC 1 cut(s) 944
BpmI CTGGAG 1 cut(s) 143
Bpu1102I GCTNAGC 2 cut(s) 137, 462
BpuEI CTTGAG 1 cut(s) 703
BpuMI CCSGG 3 cut(s) 126, 214, 386
BsaBI GATNNNNATC 1 cut(s) 531
BsaI GGTCTC 1 cut(s) 681
BsaJI CCNNGG 1 cut(s) 67
Bsc4I CCNNNNNNNGG 4 cut(s) 68, 166, 283, 863
Bse1I ACTGG 5 cut(s) 505, 615, 642, 832, 903
Bse21I CCTNAGG 1 cut(s) 964
Bse8I GATNNNNATC 1 cut(s) 531
BseDI CCNNGG 1 cut(s) 67
BseGI GGATG 4 cut(s) 175, 566, 634, 646
BseJI GATNNNNATC 1 cut(s) 531
BseLI CCNNNNNNNGG 4 cut(s) 68, 166, 283, 863
BseMII CTCAG 4 cut(s) 103, 151, 453, 978
BseNI ACTGG 5 cut(s) 505, 615, 642, 832, 903
BseXI GCAGC 1 cut(s) 361
BseYI CCCAGC 2 cut(s) 62, 797
Bsh1236I CGCG 1 cut(s) 882
Bsh1285I CGRYCG 1 cut(s) 868
BshFI GGCC 3 cut(s) 191, 231, 792
BsiEI CGRYCG 1 cut(s) 868
BsiHKAI GWGCWC 1 cut(s) 159
BsiSI CCGG 4 cut(s) 126, 213, 386, 908
BslFI GGGAC 3 cut(s) 229, 258, 899
BslI CCNNNNNNNGG 4 cut(s) 68, 166, 283, 863
BsmAI GTCTC 2 cut(s) 465, 681
BsmFI GGGAC 3 cut(s) 229, 258, 899
BsnI GGCC 3 cut(s) 191, 231, 792
Bso31I GGTCTC 1 cut(s) 681
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 2 cut(s) 532, 928
Bsp1720I GCTNAGC 2 cut(s) 137, 462
Bsp19I CCATGG 1 cut(s) 67
BspACI CCGC 7 cut(s) 240, 284, 366, 701, 787, 877, 880
BspANI GGCC 3 cut(s) 191, 231, 792
BspCNI CTCAG 4 cut(s) 102, 150, 454, 977
BspFNI CGCG 1 cut(s) 882
BspLI GGNNCC 2 cut(s) 479, 791
BspOI GCTAGC 2 cut(s) 14, 291
BspPI GGATC 1 cut(s) 923
BspTI CTTAAG 1 cut(s) 692
BspTNI GGTCTC 1 cut(s) 681
BsrI ACTGG 5 cut(s) 505, 615, 642, 832, 903
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 2 cut(s) 532, 928
BssT1I CCWWGG 1 cut(s) 67
Bst4CI ACNGT 1 cut(s) 85
BstAFI CTTAAG 1 cut(s) 692
BstC8I GCNNGC 4 cut(s) 12, 289, 459, 794
BstDEI CTNAG 4 cut(s) 89, 137, 462, 964
BstDSI CCRYGG 1 cut(s) 67
BstF5I GGATG 4 cut(s) 175, 566, 634, 646
BstFNI CGCG 1 cut(s) 882
BstH2I RGCGCY 1 cut(s) 713
BstHHI GCGC 2 cut(s) 712, 884
BstKTI GATC 2 cut(s) 535, 931
BstMAI GTCTC 2 cut(s) 465, 681
BstMBI GATC 2 cut(s) 532, 928
BstMCI CGRYCG 1 cut(s) 868
BstSCI CCNGG 3 cut(s) 124, 212, 384
BstUI CGCG 1 cut(s) 882
BstV1I GCAGC 1 cut(s) 361
BstV2I GAAGAC 1 cut(s) 944
Bsu36I CCTNAGG 1 cut(s) 964
BsuI GTATCC 1 cut(s) 764
BsuRI GGCC 3 cut(s) 191, 231, 792
BtgI CCRYGG 1 cut(s) 67
BtsCI GGATG 4 cut(s) 175, 566, 634, 646
BtsI GCAGTG 1 cut(s) 316
BtsIMutI CAGTG 2 cut(s) 316, 825
Cac8I GCNNGC 4 cut(s) 12, 289, 459, 794
CaiI CAGNNNCTG 1 cut(s) 166
CfoI GCGC 2 cut(s) 712, 884
Cfr13I GGNCC 1 cut(s) 790
Csp6I GTAC 2 cut(s) 498, 602
CviAII CATG 4 cut(s) 68, 244, 625, 758
CviQI GTAC 2 cut(s) 498, 602
DdeI CTNAG 4 cut(s) 89, 137, 462, 964
DpnI GATC 2 cut(s) 534, 930
DpnII GATC 2 cut(s) 532, 928
DriI GACNNNNNGTC 1 cut(s) 948
EaeI YGGCCR 1 cut(s) 189
Eam1105I GACNNNNNGTC 1 cut(s) 948
EciI GGCGGA 1 cut(s) 381
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 681
Eco32I GATATC 1 cut(s) 748
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 426
Eco81I CCTNAGG 1 cut(s) 964
EcoICRI GAGCTC 1 cut(s) 157
EcoRV GATATC 1 cut(s) 748
EcoT14I CCWWGG 1 cut(s) 67
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 67
FaeI CATG 4 cut(s) 71, 247, 628, 761
FaiI YATR 9 cut(s) 69, 245, 401, 525, 600, 626, 659, 759, 971
FaqI GGGAC 3 cut(s) 229, 258, 899
FatI CATG 4 cut(s) 67, 243, 624, 757
FauI CCCGC 2 cut(s) 694, 780
Fnu4HI GCNGC 4 cut(s) 285, 375, 877, 880
FokI GGATG 4 cut(s) 182, 573, 641, 653
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 4 cut(s) 285, 375, 877, 880
FspBI CTAG 5 cut(s) 11, 15, 146, 219, 288
GlaI GCGC 2 cut(s) 711, 883
GluI GCNGC 4 cut(s) 285, 375, 877, 880
GsaI CCCAGC 2 cut(s) 66, 801
GsuI CTGGAG 1 cut(s) 143
HaeII RGCGCY 1 cut(s) 713
HaeIII GGCC 3 cut(s) 191, 231, 792
HapII CCGG 4 cut(s) 126, 213, 386, 908
HhaI GCGC 2 cut(s) 712, 884
Hin1II CATG 4 cut(s) 71, 247, 628, 761
Hin6I GCGC 2 cut(s) 710, 882
HinP1I GCGC 2 cut(s) 710, 882
HinfI GANTC 4 cut(s) 108, 149, 893, 920
HpaII CCGG 4 cut(s) 126, 213, 386, 908
HphI GGTGA 4 cut(s) 185, 229, 299, 620
Hpy188I TCNGA 4 cut(s) 154, 439, 450, 511
Hpy188III TCNNGA 4 cut(s) 20, 160, 424, 940
HpyAV CCTTC 1 cut(s) 908
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4IV ACGT 2 cut(s) 271, 772
HpyCH4V TGCA 3 cut(s) 39, 182, 571
HpyF3I CTNAG 4 cut(s) 89, 137, 462, 964
HpySE526I ACGT 2 cut(s) 271, 772
Hsp92II CATG 4 cut(s) 71, 247, 628, 761
HspAI GCGC 2 cut(s) 710, 882
Kzo9I GATC 2 cut(s) 532, 928
LmnI GCTCC 2 cut(s) 162, 889
Lsp1109I GCAGC 1 cut(s) 361
LweI GCATC 3 cut(s) 517, 558, 631
MaeI CTAG 5 cut(s) 11, 15, 146, 219, 288
MaeII ACGT 2 cut(s) 271, 772
MaeIII GTNAC 3 cut(s) 235, 409, 620
MalI GATC 2 cut(s) 534, 930
MboI GATC 2 cut(s) 532, 928
MboII GAAGA 2 cut(s) 322, 944
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 5 cut(s) 433, 542, 552, 652, 739
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 7 cut(s) 69, 95, 345, 700, 867, 966, 973
MseI TTAA 3 cut(s) 5, 693, 716
MslI CAYNNNNRTG 2 cut(s) 242, 525
MspA1I CMGCKG 1 cut(s) 461
MspCI CTTAAG 1 cut(s) 692
MspI CCGG 4 cut(s) 126, 213, 386, 908
MspR9I CCNGG 3 cut(s) 126, 214, 386
MvnI CGCG 1 cut(s) 882
NciI CCSGG 3 cut(s) 126, 214, 386
NcoI CCATGG 1 cut(s) 67
NdeII GATC 2 cut(s) 532, 928
NheI GCTAGC 2 cut(s) 10, 287
NlaIII CATG 4 cut(s) 71, 247, 628, 761
NlaIV GGNNCC 2 cut(s) 479, 791
NmuCI GTSAC 3 cut(s) 235, 409, 620
PfeI GAWTC 3 cut(s) 108, 893, 920
PflMI CCANNNNNTGG 1 cut(s) 166
PfoI TCCNGGA 1 cut(s) 124
PkrI GCNGC 4 cut(s) 286, 376, 878, 881
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
Psp124BI GAGCTC 1 cut(s) 159
PspFI CCCAGC 2 cut(s) 62, 797
PspN4I GGNNCC 2 cut(s) 479, 791
PspPI GGNCC 1 cut(s) 790
PstNI CAGNNNCTG 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 2 cut(s) 499, 603
RsaNI GTAC 2 cut(s) 498, 602
RseI CAYNNNNRTG 2 cut(s) 242, 525
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 3 cut(s) 5, 693, 716
SatI GCNGC 4 cut(s) 285, 375, 877, 880
Sau3AI GATC 2 cut(s) 532, 928
Sau96I GGNCC 1 cut(s) 790
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 3 cut(s) 126, 214, 386
SduI GDGCHC 1 cut(s) 159
SfaNI GCATC 3 cut(s) 517, 558, 631
SmiMI CAYNNNNRTG 2 cut(s) 242, 525
SmlI CTYRAG 2 cut(s) 682, 692
SmoI CTYRAG 2 cut(s) 682, 692
Sse9I AATT 5 cut(s) 433, 542, 552, 652, 739
SsiI CCGC 7 cut(s) 240, 284, 366, 701, 787, 877, 880
SspI AATATT 1 cut(s) 778
SspMI CTAG 5 cut(s) 11, 15, 146, 219, 288
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 3 cut(s) 124, 212, 384
StyI CCWWGG 1 cut(s) 67
TaaI ACNGT 1 cut(s) 85
TaiI ACGT 2 cut(s) 274, 775
TaqI TCGA 2 cut(s) 300, 891
TaqII GACCGA 1 cut(s) 854
TasI AATT 5 cut(s) 433, 542, 552, 652, 739
TatI WGTACW 1 cut(s) 601
TauI GCSGC 3 cut(s) 287, 879, 882
TfiI GAWTC 3 cut(s) 108, 893, 920
Tru1I TTAA 3 cut(s) 5, 693, 716
Tru9I TTAA 3 cut(s) 5, 693, 716
TscAI CASTG 2 cut(s) 323, 832
TseFI GTSAC 3 cut(s) 235, 409, 620
TseI GCWGC 1 cut(s) 374
Tsp45I GTSAC 3 cut(s) 235, 409, 620
TspDTI ATGAA 4 cut(s) 276, 495, 783, 803
TspGWI ACGGA 2 cut(s) 114, 444
TspRI CASTG 2 cut(s) 323, 832
Van91I CCANNNNNTGG 1 cut(s) 166
Vha464I CTTAAG 1 cut(s) 692
XapI RAATTY 4 cut(s) 433, 542, 552, 739
XcmI CCANNNNNNNNNTGG 3 cut(s) 167, 359, 622
XspI CTAG 5 cut(s) 11, 15, 146, 219, 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.