FvH4_3g00990

acyl-CoA--sterol O-acyltransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002564
Physical Location & Seq
Forward (+)
493835 .. 495629
1795 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00990.t1

Sequence Viewer

Length: 1080 bp
ATGGTGATGGAGCTAGGTGAGATGGGAAGCTTCATCAAGGTATGGCTCACAGTTTTCTTATCTCTCTGCTACTGCTATGCATTACCTAAGTTCGTGCCAAAAGGAATCCCAAGACTTTTGTGCATACTTCCAATTGTGTGCCTATTTCTAGACCTCCCTCTTCACTTGACCTCCGTTCATCTGGGAGGCACCACTGGTTTCTTCATTGCTTGGCTTGCCAACTTCAAGCTCTTGCTCTTCGCTTTCGGAAAAGGCCCTTTGTCTTCCGACAATTCAATCTCTCTTGCCCGTTTCATCGCCGTCGCTTGCTTGCCGATCAAGATCCAACAAGTTCAAAAACACCAAAATGGCTCCAGCAAAGAAAAACCCAAAAAGGGCCAAAAATCCCCGGTAAATTACGCAACCAAGGGTCTACTTTTGGGTTTGTTGCTACGAGTATATGACTACAGTGAGCATATTCATCCAAAGCTTCTATGGGTCATGTACTGCTTCCACATATATTTCATGTTAGAGATCATCCTAGTTGTAGTCGCAACCGTGGCTCGAGCTGTCATGGGACTTGAGCTCGAGCCACAGTTCGACGAGCCATACCTTGCCTCTTCACTCCAAGACTTTTGGGGCCGGAGGTGGAACATCATGGTCACCAGCATTCTCCGCCCCTCCGTGTACGAGCCCGTCCTCATGGCGGCGACGCGTCTAGTAGGGCGTAAGTGGGCCCCACTCCCGGCGGTTATGGGGACTTTCGTCGTGTCGGCAATCATGCACGAGCTCATATTCTACTATCTTGGGCGCATGAGTCCAACATGGCAAGTTACATGGTTCTTTCTTCTCCACGGGGTGTGTTTGGTGGTTGAGATCGCCATCAAGAAGGCGGTTTCTTCACGAAGGTTCCAGTTGCCTAGGGTTATATCGGGGCCGTTGACGGTGGTGTTCGTGATGGCCACCGTGTTCTGGCTCTTCTTGCCTGCGCTGCTCCGGTGTAAGGCGGATGTGAGGGCGTTTGAGGAGTACGCGGCTGTCGGTACATTTGTGAAGAATGTAGGTCGTGTTTTCATGTCTAAACGTGTTTTCATAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT_2

Protein Analysis

360

Amino Acids

40.66

Weight (kDa)

9.68

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT_2 PF13813 190 - 276 3.8e-17 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AccI GTMKAC 1 cut(s) 412
AccII CGCG 2 cut(s) 694, 1013
AciI CCGC 6 cut(s) 655, 686, 728, 872, 986, 1013
AclWI GGATC 1 cut(s) 316
AcoI YGGCCR 1 cut(s) 939
AdeI CACNNNGTG 1 cut(s) 838
AfaI GTAC 4 cut(s) 485, 668, 1010, 1024
AfiI CCNNNNNNNGG 5 cut(s) 374, 685, 724, 951, 982
AflIII ACRYGT 2 cut(s) 692, 1063
AgsI TTSAA 3 cut(s) 226, 276, 335
AluBI AGCT 8 cut(s) 13, 30, 229, 469, 548, 565, 769, 1076
AluI AGCT 8 cut(s) 13, 30, 229, 469, 548, 565, 769, 1076
Alw21I GWGCWC 2 cut(s) 567, 771
AlwI GGATC 1 cut(s) 316
Ama87I CYCGRG 2 cut(s) 543, 566
AoxI GGCC 6 cut(s) 253, 376, 619, 714, 914, 939
ApaI GGGCCC 1 cut(s) 718
ApeKI GCWGC 1 cut(s) 970
AspA2I CCTAGG 1 cut(s) 899
AspLEI GCGC 2 cut(s) 792, 970
AspS9I GGNCC 6 cut(s) 254, 376, 619, 714, 715, 914
AsuC2I CCSGG 2 cut(s) 389, 725
AsuHPI GGTGA 3 cut(s) 16, 29, 634
AvaI CYCGRG 2 cut(s) 543, 566
AvrII CCTAGG 1 cut(s) 899
BaeGI GKGCMC 1 cut(s) 718
BaeI ACNNNNGTAYC 2 cut(s) 1014, 1047
BalI TGGCCA 1 cut(s) 941
BanI GGYRCC 1 cut(s) 188
BanII GRGCYC 4 cut(s) 567, 675, 718, 771
BauI CACGAG 1 cut(s) 764
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 2 cut(s) 567, 771
BbvI GCAGC 1 cut(s) 957
BccI CCATC 3 cut(s) 16, 869, 931
BceAI ACGGC 2 cut(s) 284, 901
BcnI CCSGG 2 cut(s) 389, 725
BfaI CTAG 5 cut(s) 14, 149, 521, 698, 900
BfmI CTRYAG 1 cut(s) 445
BisI GCNGC 3 cut(s) 687, 971, 1014
BlnI CCTAGG 1 cut(s) 899
BlsI GCNGC 3 cut(s) 688, 972, 1015
Bme1390I CCNGG 2 cut(s) 389, 725
BmeT110I CYCGRG 2 cut(s) 543, 566
BmgT120I GGNCC 6 cut(s) 254, 376, 619, 714, 715, 914
BmiI GGNNCC 7 cut(s) 190, 352, 620, 716, 717, 890, 915
BmrFI CCNGG 2 cut(s) 389, 725
BoxI GACNNNNGTC 1 cut(s) 743
BpiI GAAGAC 1 cut(s) 255
BpmI CTGGAG 1 cut(s) 337
BpuEI CTTGAG 1 cut(s) 581
BpuMI CCSGG 2 cut(s) 389, 725
BsaBI GATNNNNATC 2 cut(s) 320, 860
BsaJI CCNNGG 5 cut(s) 387, 405, 537, 832, 899
BsaWI WCCGGW 1 cut(s) 975
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 5 cut(s) 374, 685, 724, 951, 982
Bse1I ACTGG 2 cut(s) 199, 892
Bse3DI GCAATG 1 cut(s) 204
Bse8I GATNNNNATC 2 cut(s) 320, 860
BseDI CCNNGG 5 cut(s) 387, 405, 537, 832, 899
BseGI GGATG 3 cut(s) 460, 516, 994
BseJI GATNNNNATC 2 cut(s) 320, 860
BseLI CCNNNNNNNGG 5 cut(s) 374, 685, 724, 951, 982
BseMI GCAATG 1 cut(s) 204
BseNI ACTGG 2 cut(s) 199, 892
BseRI GAGGAG 1 cut(s) 1019
BseSI GKGCMC 1 cut(s) 718
BseXI GCAGC 1 cut(s) 957
Bsh1236I CGCG 2 cut(s) 694, 1013
BshFI GGCC 6 cut(s) 255, 378, 621, 716, 916, 941
BshNI GGYRCC 1 cut(s) 188
BsiHKAI GWGCWC 2 cut(s) 567, 771
BsiHKCI CYCGRG 2 cut(s) 543, 566
BsiSI CCGG 4 cut(s) 389, 622, 725, 976
BslFI GGGAC 2 cut(s) 570, 751
BslI CCNNNNNNNGG 5 cut(s) 374, 685, 724, 951, 982
BsmFI GGGAC 2 cut(s) 570, 751
BsmI GAATGC 1 cut(s) 648
BsnI GGCC 6 cut(s) 255, 378, 621, 716, 916, 941
BsoBI CYCGRG 2 cut(s) 543, 566
Bsp120I GGGCCC 1 cut(s) 714
Bsp1286I GDGCHC 4 cut(s) 567, 675, 718, 771
Bsp143I GATC 4 cut(s) 315, 321, 513, 855
BspACI CCGC 6 cut(s) 655, 686, 728, 872, 986, 1013
BspANI GGCC 6 cut(s) 255, 378, 621, 716, 916, 941
BspFNI CGCG 2 cut(s) 694, 1013
BspLI GGNNCC 7 cut(s) 190, 352, 620, 716, 717, 890, 915
BspPI GGATC 1 cut(s) 316
BspQI GCTCTTC 2 cut(s) 242, 962
BspT107I GGYRCC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 204
BsrI ACTGG 2 cut(s) 199, 892
BssECI CCNNGG 5 cut(s) 387, 405, 537, 832, 899
BssMI GATC 4 cut(s) 315, 321, 513, 855
BssSI CACGAG 1 cut(s) 764
BssT1I CCWWGG 2 cut(s) 405, 899
Bst2BI CACGAG 1 cut(s) 764
Bst4CI ACNGT 6 cut(s) 52, 449, 538, 576, 925, 946
Bst6I CTCTTC 4 cut(s) 165, 242, 604, 962
BstC8I GCNNGC 4 cut(s) 216, 307, 311, 966
BstDEI CTNAG 2 cut(s) 87, 1077
BstDSI CCRYGG 2 cut(s) 537, 832
BstEII GGTNACC 1 cut(s) 640
BstF5I GGATG 3 cut(s) 460, 516, 994
BstFNI CGCG 2 cut(s) 694, 1013
BstHHI GCGC 2 cut(s) 792, 970
BstKTI GATC 4 cut(s) 318, 324, 516, 858
BstMBI GATC 4 cut(s) 315, 321, 513, 855
BstMWI GCNNNNNNNGC 5 cut(s) 215, 539, 654, 961, 970
BstPAI GACNNNNGTC 1 cut(s) 743
BstPI GGTNACC 1 cut(s) 640
BstSCI CCNGG 2 cut(s) 387, 723
BstSFI CTRYAG 1 cut(s) 445
BstSLI GKGCMC 1 cut(s) 718
BstUI CGCG 2 cut(s) 694, 1013
BstV1I GCAGC 1 cut(s) 957
BstV2I GAAGAC 1 cut(s) 255
BstX2I RGATCY 1 cut(s) 321
BstYI RGATCY 1 cut(s) 321
BsuRI GGCC 6 cut(s) 255, 378, 621, 716, 916, 941
BtgI CCRYGG 2 cut(s) 537, 832
BtgZI GCGATG 1 cut(s) 280
BtsCI GGATG 3 cut(s) 460, 516, 994
BtsIMutI CAGTG 2 cut(s) 192, 454
Cac8I GCNNGC 4 cut(s) 216, 307, 311, 966
CfoI GCGC 2 cut(s) 792, 970
Cfr13I GGNCC 6 cut(s) 254, 376, 619, 714, 715, 914
CseI GACGC 2 cut(s) 683, 700
Csp6I GTAC 4 cut(s) 484, 667, 1009, 1023
CviQI GTAC 4 cut(s) 484, 667, 1009, 1023
DdeI CTNAG 2 cut(s) 87, 1077
DpnI GATC 4 cut(s) 317, 323, 515, 857
DpnII GATC 4 cut(s) 315, 321, 513, 855
DraIII CACNNNGTG 1 cut(s) 838
EaeI YGGCCR 1 cut(s) 939
Eam1104I CTCTTC 4 cut(s) 165, 242, 604, 962
EarI CTCTTC 4 cut(s) 165, 242, 604, 962
EciI GGCGGA 2 cut(s) 644, 1001
Ecl136II GAGCTC 2 cut(s) 565, 769
Eco130I CCWWGG 2 cut(s) 405, 899
Eco24I GRGCYC 4 cut(s) 567, 675, 718, 771
Eco53kI GAGCTC 2 cut(s) 565, 769
Eco88I CYCGRG 2 cut(s) 543, 566
Eco91I GGTNACC 1 cut(s) 640
EcoICRI GAGCTC 2 cut(s) 565, 769
EcoO109I RGGNCCY 2 cut(s) 254, 715
EcoO65I GGTNACC 1 cut(s) 640
EcoT14I CCWWGG 2 cut(s) 405, 899
EcoT22I ATGCAT 1 cut(s) 82
EcoT38I GRGCYC 4 cut(s) 567, 675, 718, 771
ErhI CCWWGG 2 cut(s) 405, 899
FaqI GGGAC 2 cut(s) 570, 751
FblI GTMKAC 1 cut(s) 412
Fnu4HI GCNGC 3 cut(s) 687, 971, 1014
FokI GGATG 3 cut(s) 447, 503, 1001
FriOI GRGCYC 4 cut(s) 567, 675, 718, 771
Fsp4HI GCNGC 3 cut(s) 687, 971, 1014
FspBI CTAG 5 cut(s) 14, 149, 521, 698, 900
GlaI GCGC 2 cut(s) 791, 969
GluI GCNGC 3 cut(s) 687, 971, 1014
GsuI CTGGAG 1 cut(s) 337
HaeIII GGCC 6 cut(s) 255, 378, 621, 716, 916, 941
HapII CCGG 4 cut(s) 389, 622, 725, 976
HgaI GACGC 2 cut(s) 683, 700
HhaI GCGC 2 cut(s) 792, 970
Hin6I GCGC 2 cut(s) 790, 968
HinP1I GCGC 2 cut(s) 790, 968
HincII GTYRAC 1 cut(s) 921
HindII GTYRAC 1 cut(s) 921
HindIII AAGCTT 2 cut(s) 28, 467
HinfI GANTC 2 cut(s) 105, 796
HpaII CCGG 4 cut(s) 389, 622, 725, 976
HphI GGTGA 3 cut(s) 16, 29, 634
Hpy166II GTNNAC 3 cut(s) 413, 667, 921
Hpy188I TCNGA 2 cut(s) 248, 268
Hpy188III TCNNGA 5 cut(s) 149, 319, 865, 882, 934
Hpy8I GTNNAC 3 cut(s) 413, 667, 921
Hpy99I CGWCG 4 cut(s) 305, 584, 694, 749
HpyAV CCTTC 2 cut(s) 862, 879
HpyCH4III ACNGT 6 cut(s) 52, 449, 538, 576, 925, 946
HpyCH4IV ACGT 1 cut(s) 1063
HpyCH4V TGCA 3 cut(s) 80, 123, 763
HpyF10VI GCNNNNNNNGC 5 cut(s) 215, 539, 654, 961, 970
HpyF3I CTNAG 2 cut(s) 87, 1077
HpySE526I ACGT 1 cut(s) 1063
HspAI GCGC 2 cut(s) 790, 968
Kzo9I GATC 4 cut(s) 315, 321, 513, 855
LguI GCTCTTC 2 cut(s) 242, 962
LmnI GCTCC 3 cut(s) 10, 356, 978
Lsp1109I GCAGC 1 cut(s) 957
MaeI CTAG 5 cut(s) 14, 149, 521, 698, 900
MaeII ACGT 1 cut(s) 1063
MaeIII GTNAC 2 cut(s) 640, 811
MalI GATC 4 cut(s) 317, 323, 515, 857
MboI GATC 4 cut(s) 315, 321, 513, 855
MboII GAAGA 9 cut(s) 152, 193, 229, 255, 591, 818, 870, 949, 1045
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 1 cut(s) 321
MhlI GDGCHC 4 cut(s) 567, 675, 718, 771
MlsI TGGCCA 1 cut(s) 941
MluCI AATT 3 cut(s) 132, 271, 394
MluI ACGCGT 1 cut(s) 692
MluNI TGGCCA 1 cut(s) 941
MlyI GAGTC 1 cut(s) 805
MmeI TCCRAC 3 cut(s) 291, 349, 824
Mox20I TGGCCA 1 cut(s) 941
Mph1103I ATGCAT 1 cut(s) 82
MscI TGGCCA 1 cut(s) 941
MslI CAYNNNNRTG 1 cut(s) 345
Msp20I TGGCCA 1 cut(s) 941
MspI CCGG 4 cut(s) 389, 622, 725, 976
MspR9I CCNGG 2 cut(s) 389, 725
MunI CAATTG 1 cut(s) 132
Mva1269I GAATGC 1 cut(s) 648
MvnI CGCG 2 cut(s) 694, 1013
MwoI GCNNNNNNNGC 5 cut(s) 215, 539, 654, 961, 970
NciI CCSGG 2 cut(s) 389, 725
NdeII GATC 4 cut(s) 315, 321, 513, 855
NlaIV GGNNCC 7 cut(s) 190, 352, 620, 716, 717, 890, 915
NmuCI GTSAC 1 cut(s) 640
NsiI ATGCAT 1 cut(s) 82
PaeR7I CTCGAG 2 cut(s) 543, 566
PciSI GCTCTTC 2 cut(s) 242, 962
PctI GAATGC 1 cut(s) 648
PfeI GAWTC 1 cut(s) 105
PkrI GCNGC 3 cut(s) 688, 972, 1015
PleI GAGTC 1 cut(s) 804
PpsI GAGTC 1 cut(s) 804
PshAI GACNNNNGTC 1 cut(s) 743
Psp124BI GAGCTC 2 cut(s) 567, 771
PspEI GGTNACC 1 cut(s) 640
PspN4I GGNNCC 7 cut(s) 190, 352, 620, 716, 717, 890, 915
PspOMI GGGCCC 1 cut(s) 714
PspPI GGNCC 6 cut(s) 254, 376, 619, 714, 715, 914
PspXI VCTCGAGB 2 cut(s) 543, 566
PsuI RGATCY 1 cut(s) 321
RsaI GTAC 4 cut(s) 485, 668, 1010, 1024
RsaNI GTAC 4 cut(s) 484, 667, 1009, 1023
RseI CAYNNNNRTG 1 cut(s) 345
SacI GAGCTC 2 cut(s) 567, 771
SapI GCTCTTC 2 cut(s) 242, 962
SatI GCNGC 3 cut(s) 687, 971, 1014
Sau3AI GATC 4 cut(s) 315, 321, 513, 855
Sau96I GGNCC 6 cut(s) 254, 376, 619, 714, 715, 914
SchI GAGTC 1 cut(s) 805
ScrFI CCNGG 2 cut(s) 389, 725
SduI GDGCHC 4 cut(s) 567, 675, 718, 771
SfcI CTRYAG 1 cut(s) 445
Sfr274I CTCGAG 2 cut(s) 543, 566
SlaI CTCGAG 2 cut(s) 543, 566
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 3 cut(s) 543, 560, 566
SmoI CTYRAG 3 cut(s) 543, 560, 566
Sse9I AATT 3 cut(s) 132, 271, 394
SsiI CCGC 6 cut(s) 655, 686, 728, 872, 986, 1013
SspMI CTAG 5 cut(s) 14, 149, 521, 698, 900
SstI GAGCTC 2 cut(s) 567, 771
StyD4I CCNGG 2 cut(s) 387, 723
StyI CCWWGG 2 cut(s) 405, 899
TaaI ACNGT 6 cut(s) 52, 449, 538, 576, 925, 946
TaiI ACGT 1 cut(s) 1066
TaqI TCGA 3 cut(s) 544, 567, 579
TasI AATT 3 cut(s) 132, 271, 394
TatI WGTACW 1 cut(s) 483
TauI GCSGC 2 cut(s) 689, 1016
TfiI GAWTC 1 cut(s) 105
TscAI CASTG 2 cut(s) 199, 454
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 1 cut(s) 970
Tsp45I GTSAC 1 cut(s) 640
TspDTI ATGAA 8 cut(s) 22, 167, 193, 283, 449, 493, 1042, 1060
TspGWI ACGGA 2 cut(s) 163, 652
TspRI CASTG 2 cut(s) 199, 454
XbaI TCTAGA 1 cut(s) 148
XcmI CCANNNNNNNNNTGG 1 cut(s) 471
XhoI CTCGAG 2 cut(s) 543, 566
XmaJI CCTAGG 1 cut(s) 899
XmiI GTMKAC 1 cut(s) 412
XspI CTAG 5 cut(s) 14, 149, 521, 698, 900
Zsp2I ATGCAT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.