Rorug01G0022100

acyl-CoA--sterol O-acyltransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002564
Physical Location & Seq
Reverse (-)
3631267 .. 3737424
106158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0022100.1

Sequence Viewer

Length: 990 bp
ATGAGCTGCGGAGAAAACAAGGTTGTGTGTGTGACGGGAGCATCTGGTTTCATAGCATCATGGCTGGTGAAGCTCTTATTGCAACGAGGTTATACTGTCAAAGCCACGGTTCGGGACCCAGATGATGCAAAGAAAACAGAACACTTGCTTTCACTTGATGGAGCTAAGGAAAGGCTTCATCTGTTCAAAGCAGATTTATTAGAAGAAGGATCTTTTGACCCTGTCGTTGATGGATGCGAAGGTGTTTTTCATACAGCATCCCCTGTCCTATTGTCATCTACTAATCCGCAGGCAGAACTAATTGACCCTGCAGTAAAGGGAACACATAATGTTCTGAAATCATGTGCAAAATTTCAGACTGTCAAGAGAGTGGTTTTAACTTCTTCTATGGCTGCAGTAATATTGACCGGAAAACCTCTGACCTCAGATGTGGTAGTTGATGAAACATGGTTTTCGGATCCACTTTTTTGTGAGAAGAATAAGCTATGGTATATTCTTTCAAAAACTATAGCTGAGGATACTGCTTGGAAATTCGCCAAGGAAAATGGGATTGATCTGGTTTCAATAAATCCAGGGTTTGTGATTGGTCCGTTGTTACAGCCGACTCTCAATCTCTCTGTGGAAGTGATTCAGAGTCTGCTAACTGGCAAGGCATTGCCCGCTTCAAATTACAGATTCGTTGATGTTAGAGATGTTGCCTCTGCGCATATTCAAGCCTTTGAAGTTCCTTCAGCCAGTGGAAGGTATTGTTTGGTCAACCATGTTGCACACATTTCCGAGGTTCTGAAGATTATAAGACAATTTTATCCCACTTTGTACATTCCGGAAAGATATGAGGATGACAATAATCCTCATCGTATTCTTCCTTCTGACCCAAGTTATCAAGTATCCAACGAGAAGGCAAAAGGTTTAGGAATTAACTTCTTTCCTTTTGAAGTAACGGTCAAAGACACTGTTGGAAGCCTGAAGGAGAAGGGCTTCCTCAATTAG

Protein Analysis

329

Amino Acids

36.21

Weight (kDa)

6.09

Isoelectric Point (pI)

27.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 105 1.1e-08 NmrA-like family
Epimerase PF01370 9 - 246 1.7e-22 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 239 9.8e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 11 - 200 5.7e-13 Male sterility protein
GDP_Man_Dehyd PF16363 11 - 129 1.1e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 132 1.5e-11 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 794
Acc16I TGCGCA 1 cut(s) 705
AccIII TCCGGA 1 cut(s) 823
AciI CCGC 3 cut(s) 9, 287, 660
AclWI GGATC 3 cut(s) 217, 452, 465
AcsI RAATTY 2 cut(s) 350, 530
AcuI CTGAAG 3 cut(s) 714, 806, 986
AfaI GTAC 1 cut(s) 818
AfiI CCNNNNNNNGG 2 cut(s) 111, 741
AgsI TTSAA 7 cut(s) 187, 501, 564, 666, 713, 722, 935
AjnI CCWGG 1 cut(s) 571
AluBI AGCT 5 cut(s) 6, 73, 164, 484, 512
AluI AGCT 5 cut(s) 6, 73, 164, 484, 512
AlwI GGATC 3 cut(s) 217, 452, 465
AlwNI CAGNNNCTG 1 cut(s) 637
Aor13HI TCCGGA 1 cut(s) 823
ApeKI GCWGC 2 cut(s) 6, 392
ApoI RAATTY 2 cut(s) 350, 530
Asp700I GAANNNNTTC 3 cut(s) 174, 627, 977
AspLEI GCGC 1 cut(s) 706
AspS9I GGNCC 2 cut(s) 115, 587
AsuHPI GGTGA 1 cut(s) 79
AvaII GGWCC 2 cut(s) 115, 587
BamHI GGATCC 1 cut(s) 457
BarI GAAGNNNNNNTAC 2 cut(s) 850, 882
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 1 cut(s) 379
BccI CCATC 2 cut(s) 152, 224
BciT130I CCWGG 1 cut(s) 573
BciVI GTATCC 2 cut(s) 511, 898
BfmI CTRYAG 3 cut(s) 309, 393, 507
BfuI GTATCC 2 cut(s) 511, 898
BisI GCNGC 2 cut(s) 7, 393
BlsI GCNGC 2 cut(s) 8, 394
Bme1390I CCNGG 1 cut(s) 573
Bme18I GGWCC 2 cut(s) 115, 587
BmgT120I GGNCC 2 cut(s) 115, 587
BmiI GGNNCC 3 cut(s) 116, 117, 459
BmrFI CCNGG 1 cut(s) 573
BmsI GCATC 5 cut(s) 50, 65, 115, 224, 266
Bpu10I CCTNAGC 2 cut(s) 165, 513
BsaJI CCNNGG 4 cut(s) 105, 537, 572, 777
BsaWI WCCGGW 2 cut(s) 407, 823
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 741
Bse1I ACTGG 2 cut(s) 649, 735
Bse3DI GCAATG 1 cut(s) 653
BseAI TCCGGA 1 cut(s) 823
BseBI CCWGG 1 cut(s) 573
BseDI CCNNGG 4 cut(s) 105, 537, 572, 777
BseGI GGATG 3 cut(s) 239, 257, 844
BseLI CCNNNNNNNGG 2 cut(s) 111, 741
BseMI GCAATG 1 cut(s) 653
BseMII CTCAG 2 cut(s) 438, 504
BseNI ACTGG 2 cut(s) 649, 735
BseXI GCAGC 1 cut(s) 379
BsiSI CCGG 2 cut(s) 408, 824
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 2 cut(s) 111, 741
BsmFI GGGAC 1 cut(s) 128
Bsp13I TCCGGA 1 cut(s) 823
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 3 cut(s) 209, 457, 553
BspACI CCGC 3 cut(s) 9, 287, 660
BspCNI CTCAG 2 cut(s) 437, 505
BspEI TCCGGA 1 cut(s) 823
BspLI GGNNCC 3 cut(s) 116, 117, 459
BspMAI CTGCAG 2 cut(s) 313, 397
BspPI GGATC 3 cut(s) 217, 452, 465
BsrDI GCAATG 1 cut(s) 653
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 2 cut(s) 649, 735
BssECI CCNNGG 4 cut(s) 105, 537, 572, 777
BssMI GATC 3 cut(s) 209, 457, 553
BssT1I CCWWGG 1 cut(s) 537
Bst2UI CCWGG 1 cut(s) 573
Bst4CI ACNGT 5 cut(s) 97, 109, 361, 943, 955
BstAUI TGTACA 1 cut(s) 816
BstC8I GCNNGC 2 cut(s) 291, 660
BstDEI CTNAG 3 cut(s) 165, 424, 513
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 3 cut(s) 239, 257, 844
BstHHI GCGC 1 cut(s) 706
BstKTI GATC 3 cut(s) 212, 460, 556
BstMBI GATC 3 cut(s) 209, 457, 553
BstMWI GCNNNNNNNGC 3 cut(s) 70, 79, 659
BstNI CCWGG 1 cut(s) 573
BstSCI CCNGG 1 cut(s) 571
BstSFI CTRYAG 3 cut(s) 309, 393, 507
BstV1I GCAGC 1 cut(s) 379
BstX2I RGATCY 2 cut(s) 209, 457
BstYI RGATCY 2 cut(s) 209, 457
BsuI GTATCC 2 cut(s) 511, 898
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 3 cut(s) 239, 257, 844
BtsIMutI CAGTG 2 cut(s) 742, 951
Cac8I GCNNGC 2 cut(s) 291, 660
CaiI CAGNNNCTG 1 cut(s) 637
CfoI GCGC 1 cut(s) 706
Cfr13I GGNCC 2 cut(s) 115, 587
Csp6I GTAC 1 cut(s) 817
CviAII CATG 4 cut(s) 60, 342, 447, 761
CviQI GTAC 1 cut(s) 817
DdeI CTNAG 3 cut(s) 165, 424, 513
DpnI GATC 3 cut(s) 211, 459, 555
DpnII GATC 3 cut(s) 209, 457, 553
Eco130I CCWWGG 1 cut(s) 537
Eco47I GGWCC 2 cut(s) 115, 587
Eco57I CTGAAG 3 cut(s) 714, 806, 986
EcoO109I RGGNCCY 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 571
EcoT14I CCWWGG 1 cut(s) 537
ErhI CCWWGG 1 cut(s) 537
FaeI CATG 4 cut(s) 63, 345, 450, 764
FaqI GGGAC 1 cut(s) 128
FatI CATG 4 cut(s) 59, 341, 446, 760
FauI CCCGC 1 cut(s) 667
Fnu4HI GCNGC 2 cut(s) 7, 393
FokI GGATG 3 cut(s) 244, 246, 851
Fsp4HI GCNGC 2 cut(s) 7, 393
FspI TGCGCA 1 cut(s) 705
GlaI GCGC 1 cut(s) 705
GluI GCNGC 2 cut(s) 7, 393
HapII CCGG 2 cut(s) 408, 824
HhaI GCGC 1 cut(s) 706
Hin1II CATG 4 cut(s) 63, 345, 450, 764
Hin6I GCGC 1 cut(s) 704
HinP1I GCGC 1 cut(s) 704
HincII GTYRAC 1 cut(s) 757
HindII GTYRAC 1 cut(s) 757
HinfI GANTC 4 cut(s) 604, 628, 634, 675
HpaII CCGG 2 cut(s) 408, 824
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 1 cut(s) 757
Hpy188I TCNGA 9 cut(s) 336, 357, 420, 427, 457, 633, 778, 786, 871
Hpy188III TCNNGA 3 cut(s) 113, 364, 824
Hpy8I GTNNAC 1 cut(s) 757
HpyAV CCTTC 8 cut(s) 200, 233, 735, 738, 876, 892, 961, 967
HpyCH4III ACNGT 5 cut(s) 97, 109, 361, 943, 955
HpyCH4V TGCA 6 cut(s) 82, 128, 311, 347, 395, 767
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 79, 659
HpyF3I CTNAG 3 cut(s) 165, 424, 513
Hsp92II CATG 4 cut(s) 63, 345, 450, 764
HspAI GCGC 1 cut(s) 704
KflI GGGWCCC 1 cut(s) 115
Kpn2I TCCGGA 1 cut(s) 823
Kzo9I GATC 3 cut(s) 209, 457, 553
LmnI GCTCC 2 cut(s) 38, 161
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 5 cut(s) 50, 65, 115, 224, 266
MaeIII GTNAC 3 cut(s) 31, 594, 937
MalI GATC 3 cut(s) 211, 459, 555
MboI GATC 3 cut(s) 209, 457, 553
MboII GAAGA 5 cut(s) 215, 375, 487, 799, 854
MflI RGATCY 2 cut(s) 209, 457
MluCI AATT 7 cut(s) 300, 350, 530, 667, 800, 915, 985
MlyI GAGTC 2 cut(s) 598, 643
MmeI TCCRAC 2 cut(s) 915, 937
MnlI CCTC 8 cut(s) 80, 426, 433, 508, 709, 772, 829, 861
MroI TCCGGA 1 cut(s) 823
MroXI GAANNNNTTC 3 cut(s) 174, 627, 977
MseI TTAA 2 cut(s) 377, 918
MspI CCGG 2 cut(s) 408, 824
MspR9I CCNGG 1 cut(s) 573
MvaI CCWGG 1 cut(s) 573
MwoI GCNNNNNNNGC 3 cut(s) 70, 79, 659
NdeII GATC 3 cut(s) 209, 457, 553
NlaIII CATG 4 cut(s) 63, 345, 450, 764
NlaIV GGNNCC 3 cut(s) 116, 117, 459
NmuCI GTSAC 1 cut(s) 31
NsbI TGCGCA 1 cut(s) 705
PdmI GAANNNNTTC 3 cut(s) 174, 627, 977
PfeI GAWTC 2 cut(s) 628, 675
PflFI GACNNNGTC 1 cut(s) 221
PkrI GCNGC 2 cut(s) 8, 394
PleI GAGTC 2 cut(s) 598, 642
PpsI GAGTC 2 cut(s) 598, 642
PpuMI RGGWCCY 1 cut(s) 115
PsiI TTATAA 1 cut(s) 794
Psp5II RGGWCCY 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 571
PspGI CCWGG 1 cut(s) 571
PspN4I GGNNCC 3 cut(s) 116, 117, 459
PspPI GGNCC 2 cut(s) 115, 587
PspPPI RGGWCCY 1 cut(s) 115
PstI CTGCAG 2 cut(s) 313, 397
PstNI CAGNNNCTG 1 cut(s) 637
PsuI RGATCY 2 cut(s) 209, 457
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 1 cut(s) 818
RsaNI GTAC 1 cut(s) 817
SaqAI TTAA 2 cut(s) 377, 918
SatI GCNGC 2 cut(s) 7, 393
Sau3AI GATC 3 cut(s) 209, 457, 553
Sau96I GGNCC 2 cut(s) 115, 587
SchI GAGTC 2 cut(s) 598, 643
ScrFI CCNGG 1 cut(s) 573
SfaNI GCATC 5 cut(s) 50, 65, 115, 224, 266
SfcI CTRYAG 3 cut(s) 309, 393, 507
SinI GGWCC 2 cut(s) 115, 587
Sse9I AATT 7 cut(s) 300, 350, 530, 667, 800, 915, 985
SsiI CCGC 3 cut(s) 9, 287, 660
SspI AATATT 1 cut(s) 402
StyD4I CCNGG 1 cut(s) 571
StyI CCWWGG 1 cut(s) 537
TaaI ACNGT 5 cut(s) 97, 109, 361, 943, 955
TasI AATT 7 cut(s) 300, 350, 530, 667, 800, 915, 985
TatI WGTACW 1 cut(s) 816
TfiI GAWTC 2 cut(s) 628, 675
Tru1I TTAA 2 cut(s) 377, 918
Tru9I TTAA 2 cut(s) 377, 918
TscAI CASTG 2 cut(s) 742, 958
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 2 cut(s) 6, 392
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 4 cut(s) 40, 167, 239, 456
TspGWI ACGGA 1 cut(s) 579
TspRI CASTG 2 cut(s) 742, 958
Tth111I GACNNNGTC 1 cut(s) 221
VpaK11BI GGWCC 2 cut(s) 115, 587
XapI RAATTY 2 cut(s) 350, 530
XmnI GAANNNNTTC 3 cut(s) 174, 627, 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.