Rw5G001120

acyl-CoA--sterol O-acyltransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002564
Physical Location & Seq
Forward (+)
939253 .. 940352
1100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G001120.1

Sequence Viewer

Length: 1071 bp
ATGGAGCTAGGTGAGATGGGAAGCTTCATCAGGGTATGGCTCTTTGTCTTCTTATCTCTATGCTACTGCTATGCATTACCAAGGTATGTGCCAAAAGGAGTTCCAAGACTCTTGTGTATACTTCCAATTGTGTGCCTCTTTCTAGACCTTCCTCTTCACTTGGCCTCCATACATCTTGGAGGCACCACTGCTTTCTTCATTGCTTGGCTTGCCAACTTCAAGCTCTTGCTTTTCGCTTTCGGAAAAGGCCCTTTATCTTCGGACCCTTCAATCTCCTTCCCCGTTTCATCGCCGTCGCTTGCTTGCCGATCAAGATCCAACAAAGCCCAGTTCCAAAATCCCATGACCAAAATGGCCACAACAAAGAAGCCATTTCCAGGAATGCCCAAAAAGGGCCAAAAATCCCTCATAAATTACGCAACCAAGGGCCTACTTTTGGGTTTGTTGGTACGAGTATATAACTACAGTGAGCATATTCATCCAAAGCTCCTATGGGTCATGTATTGCTTCCACATTTATTTTGCATTGGAAATCATCCTGGTTGTAGTTGCAACTGTGGTTCGACTTGTCATGGGACTCGAACTCGAGCCGCAGTTCAATGAACCATACCTTGCATCTTCGCTCCAAGACTTTTGGGGCCGAAGGTGGAACATCATGGTCACCAGCATTCTCCGCCCCACCGTATACGAGCCTGTTCTCACAGTGGCAACACGTGTAGTGGGGCGCAAATGGGCCCCTCTACCGGCCGTAATGGGGACTTTTGTGGTGTCGGCAATCATGCACGAGCTCATATTCTACTACCTTGGGCGTATGAGTCCCACATGGGAAGTTACCTGGTTCTTTCTCCTCCACGGGGGTGCAGTTGCTTCACCAAGGTTCCAGTTGCCTAGGCTGATATCGGGACCGTTGACGGTTGCATTTGTGACGGCCACCGTGTTCTGGCTCTTCTTGCCTGCACTGCTCCGGTGTAAGGCGGATGTGAGGGCGTTGGAGGAGTACGCCGCCGCCGGCACATTTGTGAAGAATGTAGGTCGTGCTTTCATTTCTAACGTGATGAAATCCACGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT_2

Protein Analysis

356

Amino Acids

40.06

Weight (kDa)

9.78

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT_2 PF13813 196 - 282 2.7e-18 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 182
AccI GTMKAC 2 cut(s) 118, 684
AciI CCGC 5 cut(s) 590, 673, 974, 1002, 1005
AclWI GGATC 1 cut(s) 309
AcoI YGGCCR 3 cut(s) 354, 744, 927
AcvI CACGTG 1 cut(s) 713
AfaI GTAC 2 cut(s) 450, 998
AfiI CCNNNNNNNGG 8 cut(s) 377, 392, 436, 742, 753, 853, 939, 970
AflIII ACRYGT 2 cut(s) 710, 712
AgsI TTSAA 3 cut(s) 220, 270, 598
AjnI CCWGG 3 cut(s) 376, 537, 833
AleI CACNNNNGTG 2 cut(s) 855, 1016
AluBI AGCT 5 cut(s) 7, 24, 223, 487, 787
AluI AGCT 5 cut(s) 7, 24, 223, 487, 787
Alw21I GWGCWC 1 cut(s) 789
AlwI GGATC 1 cut(s) 309
Ama87I CYCGRG 1 cut(s) 584
AoxI GGCC 9 cut(s) 162, 247, 354, 394, 427, 637, 732, 744, 927
ApaI GGGCCC 1 cut(s) 736
AspA2I CCTAGG 1 cut(s) 887
AspLEI GCGC 1 cut(s) 726
AspS9I GGNCC 8 cut(s) 248, 262, 394, 427, 637, 732, 733, 902
AsuHPI GGTGA 3 cut(s) 23, 652, 861
AvaI CYCGRG 1 cut(s) 584
AvaII GGWCC 2 cut(s) 262, 902
AvrII CCTAGG 1 cut(s) 887
BaeGI GKGCMC 1 cut(s) 736
BalI TGGCCA 1 cut(s) 356
BanI GGYRCC 1 cut(s) 182
BanII GRGCYC 2 cut(s) 736, 789
BauI CACGAG 1 cut(s) 782
BbrPI CACGTG 1 cut(s) 713
BbsI GAAGAC 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 789
BccI CCATC 1 cut(s) 10
BceAI ACGGC 3 cut(s) 277, 731, 942
BciT130I CCWGG 3 cut(s) 378, 539, 835
BfaI CTAG 3 cut(s) 8, 143, 888
BfmI CTRYAG 1 cut(s) 463
BisI GCNGC 3 cut(s) 590, 1002, 1005
BlnI CCTAGG 1 cut(s) 887
BlsI GCNGC 3 cut(s) 591, 1003, 1006
Bme1390I CCNGG 3 cut(s) 378, 539, 835
Bme18I GGWCC 2 cut(s) 262, 902
BmeT110I CYCGRG 1 cut(s) 584
BmgT120I GGNCC 8 cut(s) 248, 262, 394, 427, 637, 732, 733, 902
BmiI GGNNCC 7 cut(s) 184, 264, 638, 734, 735, 878, 903
BmrFI CCNGG 3 cut(s) 378, 539, 835
BmrI ACTGGG 1 cut(s) 322
BmsI GCATC 1 cut(s) 623
BmuI ACTGGG 1 cut(s) 322
BpiI GAAGAC 1 cut(s) 40
BsaAI YACGTR 1 cut(s) 713
BsaBI GATNNNNATC 1 cut(s) 313
BsaJI CCNNGG 6 cut(s) 80, 423, 802, 850, 872, 887
BsaWI WCCGGW 1 cut(s) 963
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 8 cut(s) 377, 392, 436, 742, 753, 853, 939, 970
Bse118I RCCGGY 2 cut(s) 742, 1007
Bse1I ACTGG 2 cut(s) 328, 880
Bse3DI GCAATG 1 cut(s) 198
Bse8I GATNNNNATC 1 cut(s) 313
BseBI CCWGG 3 cut(s) 378, 539, 835
BseDI CCNNGG 6 cut(s) 80, 423, 802, 850, 872, 887
BseGI GGATG 3 cut(s) 478, 534, 982
BseJI GATNNNNATC 1 cut(s) 313
BseLI CCNNNNNNNGG 8 cut(s) 377, 392, 436, 742, 753, 853, 939, 970
BseMI GCAATG 1 cut(s) 198
BseNI ACTGG 2 cut(s) 328, 880
BseRI GAGGAG 2 cut(s) 836, 1007
BseSI GKGCMC 1 cut(s) 736
BseX3I CGGCCG 1 cut(s) 744
BsgI GTGCAG 2 cut(s) 879, 939
Bsh1285I CGRYCG 1 cut(s) 747
BshFI GGCC 9 cut(s) 164, 249, 356, 396, 429, 639, 734, 746, 929
BshNI GGYRCC 1 cut(s) 182
BsiEI CGRYCG 1 cut(s) 747
BsiHKAI GWGCWC 1 cut(s) 789
BsiHKCI CYCGRG 1 cut(s) 584
BsiSI CCGG 3 cut(s) 743, 964, 1008
BslFI GGGAC 4 cut(s) 588, 769, 801, 915
BslI CCNNNNNNNGG 8 cut(s) 377, 392, 436, 742, 753, 853, 939, 970
BsmFI GGGAC 4 cut(s) 588, 769, 801, 915
BsmI GAATGC 2 cut(s) 387, 666
BsnI GGCC 9 cut(s) 164, 249, 356, 396, 429, 639, 734, 746, 929
BsoBI CYCGRG 1 cut(s) 584
Bsp120I GGGCCC 1 cut(s) 732
Bsp1286I GDGCHC 2 cut(s) 736, 789
Bsp143I GATC 2 cut(s) 308, 314
BspACI CCGC 5 cut(s) 590, 673, 974, 1002, 1005
BspANI GGCC 9 cut(s) 164, 249, 356, 396, 429, 639, 734, 746, 929
BspLI GGNNCC 7 cut(s) 184, 264, 638, 734, 735, 878, 903
BspPI GGATC 1 cut(s) 309
BspQI GCTCTTC 1 cut(s) 950
BspT107I GGYRCC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 198
BsrFI RCCGGY 2 cut(s) 742, 1007
BsrI ACTGG 2 cut(s) 328, 880
BssAI RCCGGY 2 cut(s) 742, 1007
BssECI CCNNGG 6 cut(s) 80, 423, 802, 850, 872, 887
BssMI GATC 2 cut(s) 308, 314
BssNAI GTATAC 2 cut(s) 119, 685
BssSI CACGAG 1 cut(s) 782
BssT1I CCWWGG 5 cut(s) 80, 423, 802, 872, 887
Bst1107I GTATAC 2 cut(s) 119, 685
Bst2BI CACGAG 1 cut(s) 782
Bst2UI CCWGG 3 cut(s) 378, 539, 835
Bst4CI ACNGT 7 cut(s) 467, 556, 682, 703, 906, 913, 934
Bst6I CTCTTC 2 cut(s) 159, 950
BstBAI YACGTR 1 cut(s) 713
BstC8I GCNNGC 5 cut(s) 210, 300, 304, 954, 1009
BstDSI CCRYGG 1 cut(s) 850
BstEII GGTNACC 1 cut(s) 658
BstF5I GGATG 3 cut(s) 478, 534, 982
BstHHI GCGC 1 cut(s) 726
BstKTI GATC 2 cut(s) 311, 317
BstMBI GATC 2 cut(s) 308, 314
BstMCI CGRYCG 1 cut(s) 747
BstMWI GCNNNNNNNGC 4 cut(s) 209, 672, 949, 958
BstNI CCWGG 3 cut(s) 378, 539, 835
BstPI GGTNACC 1 cut(s) 658
BstSCI CCNGG 3 cut(s) 376, 537, 833
BstSFI CTRYAG 1 cut(s) 463
BstSLI GKGCMC 1 cut(s) 736
BstV2I GAAGAC 1 cut(s) 40
BstX2I RGATCY 1 cut(s) 314
BstYI RGATCY 1 cut(s) 314
BstZ17I GTATAC 2 cut(s) 119, 685
BstZI CGGCCG 1 cut(s) 744
BsuRI GGCC 9 cut(s) 164, 249, 356, 396, 429, 639, 734, 746, 929
BtgI CCRYGG 1 cut(s) 850
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 3 cut(s) 478, 534, 982
BtsI GCAGTG 2 cut(s) 186, 956
BtsIMutI CAGTG 4 cut(s) 186, 472, 708, 956
Cac8I GCNNGC 5 cut(s) 210, 300, 304, 954, 1009
CfoI GCGC 1 cut(s) 726
Cfr10I RCCGGY 2 cut(s) 742, 1007
Cfr13I GGNCC 8 cut(s) 248, 262, 394, 427, 637, 732, 733, 902
CsiI ACCWGGT 1 cut(s) 833
Csp6I GTAC 2 cut(s) 449, 997
CviAII CATG 6 cut(s) 343, 499, 571, 655, 778, 822
CviQI GTAC 2 cut(s) 449, 997
DpnI GATC 2 cut(s) 310, 316
DpnII GATC 2 cut(s) 308, 314
EaeI YGGCCR 3 cut(s) 354, 744, 927
EagI CGGCCG 1 cut(s) 744
Eam1104I CTCTTC 2 cut(s) 159, 950
EarI CTCTTC 2 cut(s) 159, 950
EciI GGCGGA 2 cut(s) 662, 989
Ecl136II GAGCTC 1 cut(s) 787
EclXI CGGCCG 1 cut(s) 744
Eco130I CCWWGG 5 cut(s) 80, 423, 802, 872, 887
Eco24I GRGCYC 2 cut(s) 736, 789
Eco32I GATATC 1 cut(s) 897
Eco47I GGWCC 2 cut(s) 262, 902
Eco52I CGGCCG 1 cut(s) 744
Eco53kI GAGCTC 1 cut(s) 787
Eco72I CACGTG 1 cut(s) 713
Eco88I CYCGRG 1 cut(s) 584
Eco91I GGTNACC 1 cut(s) 658
EcoICRI GAGCTC 1 cut(s) 787
EcoO109I RGGNCCY 3 cut(s) 248, 427, 733
EcoO65I GGTNACC 1 cut(s) 658
EcoRII CCWGG 3 cut(s) 376, 537, 833
EcoRV GATATC 1 cut(s) 897
EcoT14I CCWWGG 5 cut(s) 80, 423, 802, 872, 887
EcoT22I ATGCAT 1 cut(s) 76
EcoT38I GRGCYC 2 cut(s) 736, 789
ErhI CCWWGG 5 cut(s) 80, 423, 802, 872, 887
FaeI CATG 6 cut(s) 346, 502, 574, 658, 781, 825
FaqI GGGAC 4 cut(s) 588, 769, 801, 915
FatI CATG 6 cut(s) 342, 498, 570, 654, 777, 821
FblI GTMKAC 2 cut(s) 118, 684
Fnu4HI GCNGC 3 cut(s) 590, 1002, 1005
FokI GGATG 3 cut(s) 465, 521, 989
FriOI GRGCYC 2 cut(s) 736, 789
Fsp4HI GCNGC 3 cut(s) 590, 1002, 1005
FspBI CTAG 3 cut(s) 8, 143, 888
GlaI GCGC 1 cut(s) 725
GluI GCNGC 3 cut(s) 590, 1002, 1005
HaeIII GGCC 9 cut(s) 164, 249, 356, 396, 429, 639, 734, 746, 929
HapII CCGG 3 cut(s) 743, 964, 1008
HhaI GCGC 1 cut(s) 726
Hin1II CATG 6 cut(s) 346, 502, 574, 658, 781, 825
Hin6I GCGC 1 cut(s) 724
HinP1I GCGC 1 cut(s) 724
HincII GTYRAC 1 cut(s) 909
HindII GTYRAC 1 cut(s) 909
HindIII AAGCTT 1 cut(s) 22
HinfI GANTC 3 cut(s) 108, 576, 814
HpaII CCGG 3 cut(s) 743, 964, 1008
HphI GGTGA 3 cut(s) 23, 652, 861
Hpy166II GTNNAC 3 cut(s) 119, 685, 909
Hpy188I TCNGA 2 cut(s) 242, 262
Hpy188III TCNNGA 3 cut(s) 143, 312, 900
Hpy8I GTNNAC 3 cut(s) 119, 685, 909
Hpy99I CGWCG 1 cut(s) 298
HpyAV CCTTC 4 cut(s) 158, 276, 286, 636
HpyCH4III ACNGT 7 cut(s) 467, 556, 682, 703, 906, 913, 934
HpyCH4IV ACGT 2 cut(s) 712, 1050
HpyCH4V TGCA 8 cut(s) 74, 524, 551, 614, 781, 860, 917, 956
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 672, 949, 958
HpySE526I ACGT 2 cut(s) 712, 1050
Hsp92II CATG 6 cut(s) 346, 502, 574, 658, 781, 825
HspAI GCGC 1 cut(s) 724
KroI GCCGGC 1 cut(s) 1007
KroNI GCCGGC 1 cut(s) 1009
Kzo9I GATC 2 cut(s) 308, 314
LguI GCTCTTC 1 cut(s) 950
LmnI GCTCC 4 cut(s) 4, 492, 627, 966
LweI GCATC 1 cut(s) 623
MabI ACCWGGT 1 cut(s) 833
MaeI CTAG 3 cut(s) 8, 143, 888
MaeII ACGT 2 cut(s) 712, 1050
MaeIII GTNAC 3 cut(s) 658, 829, 922
MalI GATC 2 cut(s) 310, 316
MboI GATC 2 cut(s) 308, 314
MboII GAAGA 7 cut(s) 40, 146, 187, 249, 609, 937, 1033
MfeI CAATTG 1 cut(s) 126
MflI RGATCY 1 cut(s) 314
MhlI GDGCHC 2 cut(s) 736, 789
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 2 cut(s) 126, 412
MluNI TGGCCA 1 cut(s) 356
MlyI GAGTC 3 cut(s) 102, 570, 823
MmeI TCCRAC 2 cut(s) 342, 969
MnlI CCTC 9 cut(s) 146, 162, 173, 175, 416, 747, 857, 975, 985
Mox20I TGGCCA 1 cut(s) 356
Mph1103I ATGCAT 1 cut(s) 76
MroNI GCCGGC 1 cut(s) 1007
MscI TGGCCA 1 cut(s) 356
MslI CAYNNNNRTG 2 cut(s) 855, 1016
Msp20I TGGCCA 1 cut(s) 356
MspI CCGG 3 cut(s) 743, 964, 1008
MspR9I CCNGG 3 cut(s) 378, 539, 835
MunI CAATTG 1 cut(s) 126
Mva1269I GAATGC 2 cut(s) 387, 666
MvaI CCWGG 3 cut(s) 378, 539, 835
MwoI GCNNNNNNNGC 4 cut(s) 209, 672, 949, 958
NaeI GCCGGC 1 cut(s) 1009
NdeII GATC 2 cut(s) 308, 314
NgoMIV GCCGGC 1 cut(s) 1007
NlaIII CATG 6 cut(s) 346, 502, 574, 658, 781, 825
NlaIV GGNNCC 7 cut(s) 184, 264, 638, 734, 735, 878, 903
NmuCI GTSAC 2 cut(s) 658, 922
NsiI ATGCAT 1 cut(s) 76
OliI CACNNNNGTG 2 cut(s) 855, 1016
PaeR7I CTCGAG 1 cut(s) 584
PciSI GCTCTTC 1 cut(s) 950
PctI GAATGC 2 cut(s) 387, 666
PdiI GCCGGC 1 cut(s) 1009
PfoI TCCNGGA 1 cut(s) 376
PkrI GCNGC 3 cut(s) 591, 1003, 1006
PleI GAGTC 3 cut(s) 102, 570, 822
PmaCI CACGTG 1 cut(s) 713
PmlI CACGTG 1 cut(s) 713
PpsI GAGTC 3 cut(s) 102, 570, 822
Ppu21I YACGTR 1 cut(s) 713
Psp124BI GAGCTC 1 cut(s) 789
Psp6I CCWGG 3 cut(s) 376, 537, 833
PspCI CACGTG 1 cut(s) 713
PspEI GGTNACC 1 cut(s) 658
PspGI CCWGG 3 cut(s) 376, 537, 833
PspN4I GGNNCC 7 cut(s) 184, 264, 638, 734, 735, 878, 903
PspOMI GGGCCC 1 cut(s) 732
PspPI GGNCC 8 cut(s) 248, 262, 394, 427, 637, 732, 733, 902
PspXI VCTCGAGB 1 cut(s) 584
PsuI RGATCY 1 cut(s) 314
RsaI GTAC 2 cut(s) 450, 998
RsaNI GTAC 2 cut(s) 449, 997
RseI CAYNNNNRTG 2 cut(s) 855, 1016
SacI GAGCTC 1 cut(s) 789
SapI GCTCTTC 1 cut(s) 950
SatI GCNGC 3 cut(s) 590, 1002, 1005
Sau3AI GATC 2 cut(s) 308, 314
Sau96I GGNCC 8 cut(s) 248, 262, 394, 427, 637, 732, 733, 902
SchI GAGTC 3 cut(s) 102, 570, 823
ScrFI CCNGG 3 cut(s) 378, 539, 835
SduI GDGCHC 2 cut(s) 736, 789
SexAI ACCWGGT 1 cut(s) 833
SfaNI GCATC 1 cut(s) 623
SfcI CTRYAG 1 cut(s) 463
Sfr274I CTCGAG 1 cut(s) 584
SinI GGWCC 2 cut(s) 262, 902
SlaI CTCGAG 1 cut(s) 584
SmiMI CAYNNNNRTG 2 cut(s) 855, 1016
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
Sse9I AATT 2 cut(s) 126, 412
SsiI CCGC 5 cut(s) 590, 673, 974, 1002, 1005
SspMI CTAG 3 cut(s) 8, 143, 888
SstI GAGCTC 1 cut(s) 789
StyD4I CCNGG 3 cut(s) 376, 537, 833
StyI CCWWGG 5 cut(s) 80, 423, 802, 872, 887
TaaI ACNGT 7 cut(s) 467, 556, 682, 703, 906, 913, 934
TaiI ACGT 2 cut(s) 715, 1053
TaqI TCGA 3 cut(s) 562, 579, 585
TasI AATT 2 cut(s) 126, 412
TauI GCSGC 3 cut(s) 592, 1004, 1007
TscAI CASTG 4 cut(s) 193, 472, 708, 963
TseFI GTSAC 2 cut(s) 658, 922
Tsp45I GTSAC 2 cut(s) 658, 922
TspDTI ATGAA 7 cut(s) 16, 187, 276, 467, 615, 1030, 1070
TspRI CASTG 4 cut(s) 193, 472, 708, 963
VpaK11BI GGWCC 2 cut(s) 262, 902
XbaI TCTAGA 1 cut(s) 142
XcmI CCANNNNNNNNNTGG 2 cut(s) 349, 489
XhoI CTCGAG 1 cut(s) 584
XmaJI CCTAGG 1 cut(s) 887
XmiI GTMKAC 2 cut(s) 118, 684
XspI CTAG 3 cut(s) 8, 143, 888
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.