MD02G1299700.v1.1

acyl-CoA--sterol O-acyltransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002564
Physical Location & Seq
Reverse (-)
35459742 .. 35460968
1227 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1299700.v1.1.491

Sequence Viewer

Length: 1227 bp
ATGGAGGGTGAATTCTTCAACTTCATTCAGGTATGGTCTTCAGTGTTTGCATGTTTCTTGTACAGCCATTCAATTGGGAAATTAGTCCCAAAAGGAACAACCAGACTTTTCTGTGTCCTCCCAGTTGTCTTCCTCTTCCTTTATCTTCCTCTCAACTTCTCCTCCATGCATCTTGAAGGTGGCACTGCCTTTTTCGTTTCTTGGCTCGGCACCTTCAAGCTCCTACTTTTTGCTTTTGGCAAAGGCCCTTTGGCTTCACATCCACCAATCTCCATTGGACGTTTCTTGGCCATCGGTTGCCTTCCAATTGAGTTCCAAGAAAACCCACCTCCAAAATCCCCAAACCCCAAATTTGAAACCTACCCTTCTCCCTCAAAATCCCACCCAAATGGCCAAATGACACAAAACCCACCTCCAAAAACTCAAAACCCTAAGATTGAAACCTACACATCTTCCTCAAAATCCAGTACAAATCGTCAAAACAGAGAAAACCCACCTCAAAAATCCCAAAATTCTAAAATTGAAACCAACCCATCTCCCCAAAACGCCCAAAAATTGCCCCTAAATCAAGTAATAAAGGGCATACTTTTTGTCATTCTAATTGGTGTCCTCAAGTATGGTGAGCACTTCCACCAAAAGCTCGTATTGATCCTTCATTGCTTGTACATTTACCTTACTCTGGAAAACTTACTGGCCATTGTAACAATATTGACTCGATCTCTGTCGGGTTTGGACCTCAAGCCGCACTTCAACGACCCATATTTCTCCACGTCGCTTCAAAACTTCTGGAGCCGTAGATGGAACCTCGTGGCCCACAGCATCCTGCACTCCGCTGTGTACGAACCCACCCGCGATTACTCTGCAGGCGTCATTGGCTCCGAGTGGGCCCCTCTGCCGGCTGTCTTCGGAAGCTTTCTGGTGTCGGGGCTCATGCACGAGCACATGTACTACTGCATGGGGCGGGTGAGGCCCACGTGGGAGGTCACGTGGTTCTTCGTTTTGCACGGAGTGTGCTTGCCGGTTGAGATCGTTTTGAACAAGGCGTTGAAGGGCAGGTGCCGGTTGCCGAAGGTGATCTCGGTGCCGTTGACCCTTGGGTTTGTGGTGGTGACCTCCTATTGGCTGTTCTTTCCGCAGTTTACTCGGTGCAAGGCTGACGTTAGAATGCTTCAAGAGTACGGTGAGTTTGCCGCGTTCTTGAAGAATGTCATTCCAAGTTTCCGATAA

Protein Analysis

409

Amino Acids

46.3

Weight (kDa)

9.46

Isoelectric Point (pI)

37.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT_2 PF13813 247 - 333 9.7e-18 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1044
Acc36I ACCTGC 1 cut(s) 1044
AccB1I GGYRCC 3 cut(s) 209, 1056, 1081
AccII CGCG 2 cut(s) 852, 1193
AciI CCGC 6 cut(s) 743, 831, 850, 961, 1133, 1191
AclWI GGATC 1 cut(s) 643
AcoI YGGCCR 3 cut(s) 288, 391, 693
AcsI RAATTY 3 cut(s) 11, 350, 511
AcuI CTGAAG 1 cut(s) 24
AcvI CACGTG 2 cut(s) 975, 987
AcyI GRCGYC 1 cut(s) 867
AdeI CACNNNGTG 1 cut(s) 1009
AfaI GTAC 6 cut(s) 62, 469, 665, 839, 947, 1178
AfiI CCNNNNNNNGG 3 cut(s) 679, 895, 1119
AflIII ACRYGT 1 cut(s) 942
AjiI CACGTC 1 cut(s) 771
AluBI AGCT 3 cut(s) 220, 640, 912
AluI AGCT 3 cut(s) 220, 640, 912
Alw21I GWGCWC 2 cut(s) 627, 942
AlwI GGATC 1 cut(s) 643
AoxI GGCC 7 cut(s) 244, 288, 391, 693, 810, 885, 968
ApaI GGGCCC 1 cut(s) 889
ApoI RAATTY 3 cut(s) 11, 350, 511
AspS9I GGNCC 6 cut(s) 245, 733, 811, 885, 886, 969
AsuHPI GGTGA 6 cut(s) 20, 632, 976, 1084, 1120, 1193
AvaII GGWCC 1 cut(s) 733
BaeGI GKGCMC 1 cut(s) 889
BalI TGGCCA 3 cut(s) 290, 393, 695
BanI GGYRCC 3 cut(s) 209, 1056, 1081
BanII GRGCYC 2 cut(s) 889, 930
BarI GAAGNNNNNNTAC 2 cut(s) 636, 668
BauI CACGAG 2 cut(s) 806, 935
BbrPI CACGTG 2 cut(s) 975, 987
BbsI GAAGAC 3 cut(s) 30, 121, 895
Bbv12I GWGCWC 2 cut(s) 627, 942
BccI CCATC 3 cut(s) 299, 541, 792
BceAI ACGGC 2 cut(s) 777, 1069
BcgI CGANNNNNNTGC 4 cut(s) 842, 876, 1124, 1158
BfmI CTRYAG 1 cut(s) 861
BfuAI ACCTGC 1 cut(s) 1044
BisI GCNGC 2 cut(s) 743, 1191
BlsI GCNGC 2 cut(s) 744, 1192
Bme18I GGWCC 1 cut(s) 733
BmgBI CACGTC 1 cut(s) 771
BmgT120I GGNCC 6 cut(s) 245, 733, 811, 885, 886, 969
BmiI GGNNCC 8 cut(s) 211, 791, 803, 877, 887, 888, 1058, 1083
BmrI ACTGGG 1 cut(s) 116
BmsI GCATC 2 cut(s) 178, 828
BmuI ACTGGG 1 cut(s) 116
BpiI GAAGAC 3 cut(s) 30, 121, 895
BpmI CTGGAG 1 cut(s) 808
BpuEI CTTGAG 2 cut(s) 596, 722
BsaAI YACGTR 2 cut(s) 975, 987
BsaHI GRCGYC 1 cut(s) 867
BsaJI CCNNGG 1 cut(s) 1093
BsaXI ACNNNNNCTCC 4 cut(s) 146, 176, 971, 1001
Bsc4I CCNNNNNNNGG 3 cut(s) 679, 895, 1119
Bse118I RCCGGY 3 cut(s) 895, 1018, 1059
Bse1I ACTGG 3 cut(s) 122, 465, 696
Bse3DI GCAATG 1 cut(s) 655
BseDI CCNNGG 1 cut(s) 1093
BseGI GGATG 2 cut(s) 259, 819
BseLI CCNNNNNNNGG 3 cut(s) 679, 895, 1119
BseMI GCAATG 1 cut(s) 655
BseNI ACTGG 3 cut(s) 122, 465, 696
BseRI GAGGAG 1 cut(s) 151
BseSI GKGCMC 1 cut(s) 889
BsgI GTGCAG 1 cut(s) 809
Bsh1236I CGCG 2 cut(s) 852, 1193
BshFI GGCC 7 cut(s) 246, 290, 393, 695, 812, 887, 970
BshNI GGYRCC 3 cut(s) 209, 1056, 1081
BsiHKAI GWGCWC 2 cut(s) 627, 942
BsiSI CCGG 3 cut(s) 896, 1019, 1060
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 3 cut(s) 679, 895, 1119
BsmFI GGGAC 1 cut(s) 71
BsmI GAATGC 1 cut(s) 1170
BsnI GGCC 7 cut(s) 246, 290, 393, 695, 812, 887, 970
Bsp120I GGGCCC 1 cut(s) 885
Bsp1286I GDGCHC 4 cut(s) 627, 889, 930, 942
Bsp1407I TGTACA 2 cut(s) 60, 663
Bsp143I GATC 4 cut(s) 648, 716, 1026, 1074
BspACI CCGC 6 cut(s) 743, 831, 850, 961, 1133, 1191
BspANI GGCC 7 cut(s) 246, 290, 393, 695, 812, 887, 970
BspFNI CGCG 2 cut(s) 852, 1193
BspLI GGNNCC 8 cut(s) 211, 791, 803, 877, 887, 888, 1058, 1083
BspMAI CTGCAG 1 cut(s) 865
BspMI ACCTGC 1 cut(s) 1044
BspPI GGATC 1 cut(s) 643
BspT107I GGYRCC 3 cut(s) 209, 1056, 1081
BsrDI GCAATG 1 cut(s) 655
BsrFI RCCGGY 3 cut(s) 895, 1018, 1059
BsrGI TGTACA 2 cut(s) 60, 663
BsrI ACTGG 3 cut(s) 122, 465, 696
BssAI RCCGGY 3 cut(s) 895, 1018, 1059
BssECI CCNNGG 1 cut(s) 1093
BssMI GATC 4 cut(s) 648, 716, 1026, 1074
BssNI GRCGYC 1 cut(s) 867
BssSI CACGAG 2 cut(s) 806, 935
BssT1I CCWWGG 1 cut(s) 1093
Bst2BI CACGAG 2 cut(s) 806, 935
Bst4CI ACNGT 1 cut(s) 1181
Bst6I CTCTTC 1 cut(s) 140
BstACI GRCGYC 1 cut(s) 867
BstAUI TGTACA 2 cut(s) 60, 663
BstBAI YACGTR 2 cut(s) 975, 987
BstC8I GCNNGC 3 cut(s) 865, 897, 1016
BstDEI CTNAG 1 cut(s) 432
BstEII GGTNACC 1 cut(s) 1108
BstF5I GGATG 2 cut(s) 259, 819
BstFNI CGCG 2 cut(s) 852, 1193
BstKTI GATC 4 cut(s) 651, 719, 1029, 1077
BstMBI GATC 4 cut(s) 648, 716, 1026, 1074
BstMWI GCNNNNNNNGC 2 cut(s) 873, 967
BstNSI RCATGY 2 cut(s) 54, 946
BstPI GGTNACC 1 cut(s) 1108
BstSFI CTRYAG 1 cut(s) 861
BstSLI GKGCMC 1 cut(s) 889
BstUI CGCG 2 cut(s) 852, 1193
BstV2I GAAGAC 3 cut(s) 30, 121, 895
BstXI CCANNNNNNTGG 2 cut(s) 74, 389
BsuRI GGCC 7 cut(s) 246, 290, 393, 695, 812, 887, 970
BtrI CACGTC 1 cut(s) 771
BtsCI GGATG 2 cut(s) 259, 819
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 2 cut(s) 48, 183
BveI ACCTGC 1 cut(s) 1044
Cac8I GCNNGC 3 cut(s) 865, 897, 1016
Cfr10I RCCGGY 3 cut(s) 895, 1018, 1059
Cfr13I GGNCC 6 cut(s) 245, 733, 811, 885, 886, 969
CseI GACGC 1 cut(s) 856
Csp6I GTAC 6 cut(s) 61, 468, 664, 838, 946, 1177
CviAII CATG 5 cut(s) 51, 166, 931, 943, 955
CviQI GTAC 6 cut(s) 61, 468, 664, 838, 946, 1177
DdeI CTNAG 1 cut(s) 432
DpnI GATC 4 cut(s) 650, 718, 1028, 1076
DpnII GATC 4 cut(s) 648, 716, 1026, 1074
DraIII CACNNNGTG 1 cut(s) 1009
EaeI YGGCCR 3 cut(s) 288, 391, 693
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 1093
Eco24I GRGCYC 2 cut(s) 889, 930
Eco47I GGWCC 1 cut(s) 733
Eco57I CTGAAG 1 cut(s) 24
Eco72I CACGTG 2 cut(s) 975, 987
Eco91I GGTNACC 1 cut(s) 1108
EcoO109I RGGNCCY 2 cut(s) 245, 886
EcoO65I GGTNACC 1 cut(s) 1108
EcoRI GAATTC 1 cut(s) 11
EcoT14I CCWWGG 1 cut(s) 1093
EcoT22I ATGCAT 1 cut(s) 171
EcoT38I GRGCYC 2 cut(s) 889, 930
ErhI CCWWGG 1 cut(s) 1093
FaeI CATG 5 cut(s) 54, 169, 934, 946, 958
FaiI YATR 9 cut(s) 34, 52, 167, 584, 618, 760, 932, 944, 956
FalI AAGNNNNNCTT 2 cut(s) 731, 763
FaqI GGGAC 1 cut(s) 71
FatI CATG 5 cut(s) 50, 165, 930, 942, 954
FauI CCCGC 2 cut(s) 857, 954
Fnu4HI GCNGC 2 cut(s) 743, 1191
FokI GGATG 2 cut(s) 246, 806
FriOI GRGCYC 2 cut(s) 889, 930
Fsp4HI GCNGC 2 cut(s) 743, 1191
GluI GCNGC 2 cut(s) 743, 1191
GsuI CTGGAG 1 cut(s) 808
HaeIII GGCC 7 cut(s) 246, 290, 393, 695, 812, 887, 970
HapII CCGG 3 cut(s) 896, 1019, 1060
HgaI GACGC 1 cut(s) 856
Hin1I GRCGYC 1 cut(s) 867
Hin1II CATG 5 cut(s) 54, 169, 934, 946, 958
HincII GTYRAC 1 cut(s) 1089
HindII GTYRAC 1 cut(s) 1089
HindIII AAGCTT 1 cut(s) 910
HinfI GANTC 1 cut(s) 712
HpaII CCGG 3 cut(s) 896, 1019, 1060
HphI GGTGA 6 cut(s) 20, 632, 976, 1084, 1120, 1193
Hpy166II GTNNAC 3 cut(s) 838, 1089, 1140
Hpy188I TCNGA 3 cut(s) 880, 908, 1223
Hpy188III TCNNGA 5 cut(s) 173, 680, 787, 1172, 1198
Hpy8I GTNNAC 3 cut(s) 838, 1089, 1140
Hpy99I CGWCG 1 cut(s) 775
HpyAV CCTTC 7 cut(s) 170, 223, 311, 375, 662, 1042, 1063
HpyCH4III ACNGT 1 cut(s) 1181
HpyCH4IV ACGT 5 cut(s) 280, 770, 974, 986, 1158
HpyCH4V TGCA 8 cut(s) 50, 169, 826, 863, 934, 954, 1003, 1149
HpyF10VI GCNNNNNNNGC 2 cut(s) 873, 967
HpyF3I CTNAG 1 cut(s) 432
HpySE526I ACGT 5 cut(s) 280, 770, 974, 986, 1158
Hsp92I GRCGYC 1 cut(s) 867
Hsp92II CATG 5 cut(s) 54, 169, 934, 946, 958
KroI GCCGGC 1 cut(s) 895
KroNI GCCGGC 1 cut(s) 897
Kzo9I GATC 4 cut(s) 648, 716, 1026, 1074
LmnI GCTCC 3 cut(s) 225, 789, 881
LweI GCATC 2 cut(s) 178, 828
MaeII ACGT 5 cut(s) 280, 770, 974, 986, 1158
MaeIII GTNAC 3 cut(s) 700, 982, 1108
MalI GATC 4 cut(s) 650, 718, 1028, 1076
MboI GATC 4 cut(s) 648, 716, 1026, 1074
MboII GAAGA 9 cut(s) 7, 30, 121, 127, 137, 444, 895, 985, 1213
MfeI CAATTG 2 cut(s) 72, 306
MhlI GDGCHC 4 cut(s) 627, 889, 930, 942
MlsI TGGCCA 3 cut(s) 290, 393, 695
MluCI AATT 9 cut(s) 11, 72, 80, 306, 350, 511, 519, 554, 600
MluNI TGGCCA 3 cut(s) 290, 393, 695
MlyI GAGTC 1 cut(s) 706
Mox20I TGGCCA 3 cut(s) 290, 393, 695
Mph1103I ATGCAT 1 cut(s) 171
MroNI GCCGGC 1 cut(s) 895
MscI TGGCCA 3 cut(s) 290, 393, 695
MslI CAYNNNNRTG 1 cut(s) 387
Msp20I TGGCCA 3 cut(s) 290, 393, 695
MspA1I CMGCKG 1 cut(s) 833
MspI CCGG 3 cut(s) 896, 1019, 1060
MunI CAATTG 2 cut(s) 72, 306
Mva1269I GAATGC 1 cut(s) 1170
MvnI CGCG 2 cut(s) 852, 1193
MwoI GCNNNNNNNGC 2 cut(s) 873, 967
NaeI GCCGGC 1 cut(s) 897
NdeII GATC 4 cut(s) 648, 716, 1026, 1074
NgoMIV GCCGGC 1 cut(s) 895
NlaIII CATG 5 cut(s) 54, 169, 934, 946, 958
NlaIV GGNNCC 8 cut(s) 211, 791, 803, 877, 887, 888, 1058, 1083
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 2 cut(s) 982, 1108
NsiI ATGCAT 1 cut(s) 171
NspI RCATGY 2 cut(s) 54, 946
PaqCI CACCTGC 1 cut(s) 1044
PciI ACATGT 1 cut(s) 942
PctI GAATGC 1 cut(s) 1170
PdiI GCCGGC 1 cut(s) 897
PkrI GCNGC 2 cut(s) 744, 1192
PleI GAGTC 1 cut(s) 706
PmaCI CACGTG 2 cut(s) 975, 987
PmlI CACGTG 2 cut(s) 975, 987
PpsI GAGTC 1 cut(s) 706
Ppu21I YACGTR 2 cut(s) 975, 987
PscI ACATGT 1 cut(s) 942
PspCI CACGTG 2 cut(s) 975, 987
PspEI GGTNACC 1 cut(s) 1108
PspN4I GGNNCC 8 cut(s) 211, 791, 803, 877, 887, 888, 1058, 1083
PspOMI GGGCCC 1 cut(s) 885
PspPI GGNCC 6 cut(s) 245, 733, 811, 885, 886, 969
PstI CTGCAG 1 cut(s) 865
RsaI GTAC 6 cut(s) 62, 469, 665, 839, 947, 1178
RsaNI GTAC 6 cut(s) 61, 468, 664, 838, 946, 1177
RseI CAYNNNNRTG 1 cut(s) 387
SatI GCNGC 2 cut(s) 743, 1191
Sau3AI GATC 4 cut(s) 648, 716, 1026, 1074
Sau96I GGNCC 6 cut(s) 245, 733, 811, 885, 886, 969
SchI GAGTC 1 cut(s) 706
SduI GDGCHC 4 cut(s) 627, 889, 930, 942
SfaNI GCATC 2 cut(s) 178, 828
SfcI CTRYAG 1 cut(s) 861
SinI GGWCC 1 cut(s) 733
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 2 cut(s) 611, 737
SmoI CTYRAG 2 cut(s) 611, 737
Sse9I AATT 9 cut(s) 11, 72, 80, 306, 350, 511, 519, 554, 600
SsiI CCGC 6 cut(s) 743, 831, 850, 961, 1133, 1191
SspI AATATT 1 cut(s) 708
StyI CCWWGG 1 cut(s) 1093
TaaI ACNGT 1 cut(s) 1181
TaiI ACGT 5 cut(s) 283, 773, 977, 989, 1161
TaqI TCGA 1 cut(s) 715
TasI AATT 9 cut(s) 11, 72, 80, 306, 350, 511, 519, 554, 600
TatI WGTACW 4 cut(s) 60, 467, 663, 945
TauI GCSGC 2 cut(s) 745, 1193
TscAI CASTG 2 cut(s) 48, 190
TseFI GTSAC 2 cut(s) 982, 1108
Tsp45I GTSAC 2 cut(s) 982, 1108
TspDTI ATGAA 2 cut(s) 13, 644
TspGWI ACGGA 1 cut(s) 1020
TspRI CASTG 2 cut(s) 48, 190
VpaK11BI GGWCC 1 cut(s) 733
XapI RAATTY 3 cut(s) 11, 350, 511
XceI RCATGY 2 cut(s) 54, 946
Zsp2I ATGCAT 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.