MD10G1331600.v1.1

acyl-CoA--sterol O-acyltransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002564
Physical Location & Seq
Reverse (-)
40938921 .. 40940027
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1331600.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGGAGGGTGAGATTGGAAACTTCGTCAAGGTATGGCTCTCAGTTTACCTATCTCTATGTTACTGCTATGCAATTGCTAAGTTTGTGCCAAAAGGAGTTCCAAGACTTTTGCTCATAATTCCGATCGTGTCCCTCTTCTTATACCTCCCTCTCCACCTCTCCTCCCTCCATCTCGGCGGCACCACCGCTTTCTTCATTTCTTGGCTTGCAAACTTCAAGCTCTTGCTTTTCGCTTTCGGCAAAGGCCCTCTCTGCTCCGACCCTTCGATATCTCTTCTCCGTTTCGTGGCGGTTGCTTGCTTACCCATCAAAATTCAGCAAAACCAGTATTCAAAAACCCAAAACAAAGAAAACCCAGCCCCGAAAATAACCAAAAATGGAACAAAATCCCCATTAAACTACGCAGTTAAAGGCCTATTGTTTGCCTTGTTGGTTAAGCTATATGACTACAAAGAGCACATTCATCCAAAGCTCTTATGGATCATGTATTGCTTCCACATATACTTCGCCTTGGAAATAATCCTAGTCATCGCCGCCACGTTGGCTCGAGCGCTTCTGGGGCTGGAGCTTGAGCCCCAATTCGACGAGCCTTACCTCTCGTCCTCGCTCCAGGACTTCTGGGGTAGGCGATGGAACATCATGGTCACCAGCATCCTGCGCCCAACCGTGTATGAGCCTATCCTCGCGCTAGCAACTCGTGTGGTCGGGCGCAGGTGGGCTACTCTCGCAGGCGTGATGGGGACGTTTTTGGTGTCCGCAATCATGCACGAGCTCATATTCTACTACCTGGGCAGGACGCGGCCCACTTGGGAGATCACGTGGTTCTTCCTCCTCCACGGGGCGTGCTTGGTGGCTGAGATCGGCGTCAAGAAGGCGGTCGGGAAACGGAAGAAGCTGCAGCTGCCGAGGTCAGTGTCCGGGCCGTTGACCGTCGCGTTTGTGATGGCCACCGTGTTTTGGCTCTTCTTTCCTCCTCTGCTGCGGTGTAAGGCGGACGTGAGGGCATTGGAGGAGTACGCGGCTGTTGGCGCGTTTGTGAAGAATATCGGCCATGCTTTCGCTTCTACAGCGGTGAACGCAACAGCGACAATAACAAGAACCTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT_2

Protein Analysis

369

Amino Acids

41.34

Weight (kDa)

9.69

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT_2 PF13813 191 - 277 6.1e-18 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 702
Acc36I ACCTGC 1 cut(s) 702
AccB1I GGYRCC 1 cut(s) 179
AccII CGCG 5 cut(s) 686, 799, 935, 1019, 1031
AclWI GGATC 1 cut(s) 488
AcoI YGGCCR 2 cut(s) 945, 1048
AcsI RAATTY 1 cut(s) 312
AcvI CACGTG 1 cut(s) 819
AcyI GRCGYC 1 cut(s) 864
AfaI GTAC 1 cut(s) 1016
AfeI AGCGCT 1 cut(s) 552
AfiI CCNNNNNNNGG 3 cut(s) 286, 838, 957
AgsI TTSAA 2 cut(s) 217, 333
AjiI CACGTC 1 cut(s) 997
AjnI CCWGG 2 cut(s) 609, 786
AluBI AGCT 7 cut(s) 220, 439, 472, 568, 772, 895, 901
AluI AGCT 7 cut(s) 220, 439, 472, 568, 772, 895, 901
Alw21I GWGCWC 2 cut(s) 459, 774
AlwI GGATC 1 cut(s) 488
Ama87I CYCGRG 1 cut(s) 546
Aor51HI AGCGCT 1 cut(s) 552
AoxI GGCC 6 cut(s) 244, 412, 800, 920, 945, 1048
ApeKI GCWGC 4 cut(s) 895, 898, 901, 979
ApoI RAATTY 1 cut(s) 312
AspLEI GCGC 5 cut(s) 553, 660, 688, 711, 1031
AspS9I GGNCC 3 cut(s) 245, 801, 920
AsuC2I CCSGG 1 cut(s) 919
AsuHPI GGTGA 3 cut(s) 20, 637, 1084
AsuNHI GCTAGC 1 cut(s) 688
AvaI CYCGRG 1 cut(s) 546
BalI TGGCCA 1 cut(s) 947
BanI GGYRCC 1 cut(s) 179
BanII GRGCYC 2 cut(s) 576, 774
BauI CACGAG 2 cut(s) 696, 767
BbrPI CACGTG 1 cut(s) 819
Bbv12I GWGCWC 2 cut(s) 459, 774
BbvI GCAGC 4 cut(s) 882, 888, 910, 966
BccI CCATC 5 cut(s) 177, 314, 624, 730, 937
BceAI ACGGC 1 cut(s) 907
BciT130I CCWGG 2 cut(s) 611, 788
BcnI CCSGG 1 cut(s) 919
BfaI CTAG 2 cut(s) 524, 689
BfmI CTRYAG 2 cut(s) 896, 1065
BfoI RGCGCY 1 cut(s) 554
BfuAI ACCTGC 1 cut(s) 702
BglI GCCNNNNNGGC 1 cut(s) 542
BisI GCNGC 8 cut(s) 178, 534, 800, 896, 899, 902, 980, 1020
BlsI GCNGC 8 cut(s) 179, 535, 801, 897, 900, 903, 981, 1021
Bme1390I CCNGG 3 cut(s) 611, 788, 919
BmeT110I CYCGRG 1 cut(s) 546
BmgBI CACGTC 1 cut(s) 997
BmgT120I GGNCC 3 cut(s) 245, 801, 920
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 3 cut(s) 611, 788, 919
BmsI GCATC 1 cut(s) 660
BmtI GCTAGC 1 cut(s) 692
BpmI CTGGAG 2 cut(s) 584, 593
BpuEI CTTGAG 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 919
BsaAI YACGTR 1 cut(s) 819
BsaHI GRCGYC 1 cut(s) 864
BsaJI CCNNGG 4 cut(s) 510, 787, 835, 905
BsaXI ACNNNNNCTCC 4 cut(s) 146, 176, 803, 833
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 838, 957
Bse1I ACTGG 1 cut(s) 325
BseBI CCWGG 2 cut(s) 611, 788
BseDI CCNNGG 4 cut(s) 510, 787, 835, 905
BseGI GGATG 2 cut(s) 463, 651
BseLI CCNNNNNNNGG 3 cut(s) 286, 838, 957
BseMII CTCAG 2 cut(s) 54, 846
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 4 cut(s) 151, 821, 963, 1025
BseXI GCAGC 4 cut(s) 882, 888, 910, 966
BseYI CCCAGC 1 cut(s) 355
Bsh1236I CGCG 5 cut(s) 686, 799, 935, 1019, 1031
Bsh1285I CGRYCG 2 cut(s) 126, 879
BshFI GGCC 6 cut(s) 246, 414, 802, 922, 947, 1050
BshNI GGYRCC 1 cut(s) 179
BsiEI CGRYCG 2 cut(s) 126, 879
BsiHKAI GWGCWC 2 cut(s) 459, 774
BsiHKCI CYCGRG 1 cut(s) 546
BsiSI CCGG 1 cut(s) 918
BslFI GGGAC 2 cut(s) 115, 754
BslI CCNNNNNNNGG 3 cut(s) 286, 838, 957
BsmFI GGGAC 2 cut(s) 115, 754
BsnI GGCC 6 cut(s) 246, 414, 802, 922, 947, 1050
BsoBI CYCGRG 1 cut(s) 546
Bsp1286I GDGCHC 3 cut(s) 459, 576, 774
Bsp143I GATC 4 cut(s) 123, 480, 813, 858
BspANI GGCC 6 cut(s) 246, 414, 802, 922, 947, 1050
BspCNI CTCAG 2 cut(s) 53, 847
BspFNI CGCG 5 cut(s) 686, 799, 935, 1019, 1031
BspLI GGNNCC 1 cut(s) 181
BspMAI CTGCAG 1 cut(s) 900
BspMI ACCTGC 1 cut(s) 702
BspOI GCTAGC 1 cut(s) 692
BspPI GGATC 1 cut(s) 488
BspQI GCTCTTC 1 cut(s) 968
BspT107I GGYRCC 1 cut(s) 179
BsrI ACTGG 1 cut(s) 325
BssECI CCNNGG 4 cut(s) 510, 787, 835, 905
BssMI GATC 4 cut(s) 123, 480, 813, 858
BssNI GRCGYC 1 cut(s) 864
BssSI CACGAG 2 cut(s) 696, 767
BssT1I CCWWGG 1 cut(s) 510
Bst2BI CACGAG 2 cut(s) 696, 767
Bst2UI CCWGG 2 cut(s) 611, 788
Bst4CI ACNGT 3 cut(s) 667, 931, 952
Bst6I CTCTTC 3 cut(s) 140, 279, 968
BstACI GRCGYC 1 cut(s) 864
BstBAI YACGTR 1 cut(s) 819
BstC8I GCNNGC 5 cut(s) 207, 298, 690, 730, 844
BstDEI CTNAG 3 cut(s) 40, 78, 855
BstDSI CCRYGG 1 cut(s) 835
BstEII GGTNACC 1 cut(s) 643
BstF5I GGATG 2 cut(s) 463, 651
BstFNI CGCG 5 cut(s) 686, 799, 935, 1019, 1031
BstH2I RGCGCY 1 cut(s) 554
BstHHI GCGC 5 cut(s) 553, 660, 688, 711, 1031
BstKTI GATC 4 cut(s) 126, 483, 816, 861
BstMBI GATC 4 cut(s) 123, 480, 813, 858
BstMCI CGRYCG 2 cut(s) 126, 879
BstMWI GCNNNNNNNGC 9 cut(s) 252, 542, 559, 657, 725, 901, 1028, 1067, 1076
BstNI CCWGG 2 cut(s) 611, 788
BstPI GGTNACC 1 cut(s) 643
BstSCI CCNGG 3 cut(s) 609, 786, 917
BstSFI CTRYAG 2 cut(s) 896, 1065
BstUI CGCG 5 cut(s) 686, 799, 935, 1019, 1031
BstV1I GCAGC 4 cut(s) 882, 888, 910, 966
BsuRI GGCC 6 cut(s) 246, 414, 802, 922, 947, 1050
BtgI CCRYGG 1 cut(s) 835
BtgZI GCGATG 2 cut(s) 514, 643
BtrI CACGTC 1 cut(s) 997
BtsCI GGATG 2 cut(s) 463, 651
BtsIMutI CAGTG 1 cut(s) 918
BveI ACCTGC 1 cut(s) 702
Cac8I GCNNGC 5 cut(s) 207, 298, 690, 730, 844
CfoI GCGC 5 cut(s) 553, 660, 688, 711, 1031
Cfr13I GGNCC 3 cut(s) 245, 801, 920
CseI GACGC 2 cut(s) 805, 853
Csp6I GTAC 1 cut(s) 1015
CspCI CAANNNNNGTGG 2 cut(s) 681, 716
CviAII CATG 4 cut(s) 484, 640, 763, 1052
CviQI GTAC 1 cut(s) 1015
DdeI CTNAG 3 cut(s) 40, 78, 855
DpnI GATC 4 cut(s) 125, 482, 815, 860
DpnII GATC 4 cut(s) 123, 480, 813, 858
EaeI YGGCCR 2 cut(s) 945, 1048
Eam1104I CTCTTC 3 cut(s) 140, 279, 968
EarI CTCTTC 3 cut(s) 140, 279, 968
EciI GGCGGA 1 cut(s) 1007
Ecl136II GAGCTC 1 cut(s) 772
Eco130I CCWWGG 1 cut(s) 510
Eco147I AGGCCT 1 cut(s) 414
Eco24I GRGCYC 2 cut(s) 576, 774
Eco32I GATATC 1 cut(s) 270
Eco47III AGCGCT 1 cut(s) 552
Eco53kI GAGCTC 1 cut(s) 772
Eco72I CACGTG 1 cut(s) 819
Eco88I CYCGRG 1 cut(s) 546
Eco91I GGTNACC 1 cut(s) 643
EcoICRI GAGCTC 1 cut(s) 772
EcoO109I RGGNCCY 1 cut(s) 245
EcoO65I GGTNACC 1 cut(s) 643
EcoRII CCWGG 2 cut(s) 609, 786
EcoRV GATATC 1 cut(s) 270
EcoT14I CCWWGG 1 cut(s) 510
EcoT38I GRGCYC 2 cut(s) 576, 774
ErhI CCWWGG 1 cut(s) 510
FaeI CATG 4 cut(s) 487, 643, 766, 1055
FaqI GGGAC 2 cut(s) 115, 754
FatI CATG 4 cut(s) 483, 639, 762, 1051
Fnu4HI GCNGC 8 cut(s) 178, 534, 800, 896, 899, 902, 980, 1020
FokI GGATG 2 cut(s) 450, 638
FriOI GRGCYC 2 cut(s) 576, 774
Fsp4HI GCNGC 8 cut(s) 178, 534, 800, 896, 899, 902, 980, 1020
FspBI CTAG 2 cut(s) 524, 689
GlaI GCGC 5 cut(s) 552, 659, 687, 710, 1030
GluI GCNGC 8 cut(s) 178, 534, 800, 896, 899, 902, 980, 1020
GsaI CCCAGC 1 cut(s) 359
GsuI CTGGAG 2 cut(s) 584, 593
HaeII RGCGCY 1 cut(s) 554
HaeIII GGCC 6 cut(s) 246, 414, 802, 922, 947, 1050
HapII CCGG 1 cut(s) 918
HgaI GACGC 2 cut(s) 805, 853
HhaI GCGC 5 cut(s) 553, 660, 688, 711, 1031
Hin1I GRCGYC 1 cut(s) 864
Hin1II CATG 4 cut(s) 487, 643, 766, 1055
Hin6I GCGC 5 cut(s) 551, 658, 686, 709, 1029
HinP1I GCGC 5 cut(s) 551, 658, 686, 709, 1029
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HpaII CCGG 1 cut(s) 918
HphI GGTGA 3 cut(s) 20, 637, 1084
Hpy166II GTNNAC 3 cut(s) 46, 927, 1075
Hpy188I TCNGA 2 cut(s) 123, 259
Hpy188III TCNNGA 2 cut(s) 868, 880
Hpy8I GTNNAC 3 cut(s) 46, 927, 1075
Hpy99I CGWCG 2 cut(s) 587, 935
HpyAV CCTTC 2 cut(s) 273, 865
HpyCH4III ACNGT 3 cut(s) 667, 931, 952
HpyCH4IV ACGT 4 cut(s) 539, 743, 818, 996
HpyCH4V TGCA 4 cut(s) 71, 209, 766, 898
HpyF10VI GCNNNNNNNGC 9 cut(s) 252, 542, 559, 657, 725, 901, 1028, 1067, 1076
HpyF3I CTNAG 3 cut(s) 40, 78, 855
HpySE526I ACGT 4 cut(s) 539, 743, 818, 996
Hsp92I GRCGYC 1 cut(s) 864
Hsp92II CATG 4 cut(s) 487, 643, 766, 1055
HspAI GCGC 5 cut(s) 551, 658, 686, 709, 1029
Kzo9I GATC 4 cut(s) 123, 480, 813, 858
LguI GCTCTTC 1 cut(s) 968
LmnI GCTCC 3 cut(s) 260, 565, 612
Lsp1109I GCAGC 4 cut(s) 882, 888, 910, 966
LweI GCATC 1 cut(s) 660
MaeI CTAG 2 cut(s) 524, 689
MaeII ACGT 4 cut(s) 539, 743, 818, 996
MaeIII GTNAC 2 cut(s) 59, 643
MalI GATC 4 cut(s) 125, 482, 815, 860
MboI GATC 4 cut(s) 123, 480, 813, 858
MboII GAAGA 7 cut(s) 127, 184, 266, 817, 901, 955, 1051
MfeI CAATTG 1 cut(s) 72
MhlI GDGCHC 3 cut(s) 459, 576, 774
MlsI TGGCCA 1 cut(s) 947
MluCI AATT 4 cut(s) 72, 117, 312, 578
MluNI TGGCCA 1 cut(s) 947
MmeI TCCRAC 1 cut(s) 282
Mox20I TGGCCA 1 cut(s) 947
MscI TGGCCA 1 cut(s) 947
MseI TTAA 3 cut(s) 395, 408, 435
Msp20I TGGCCA 1 cut(s) 947
MspA1I CMGCKG 2 cut(s) 901, 1070
MspI CCGG 1 cut(s) 918
MspR9I CCNGG 3 cut(s) 611, 788, 919
MunI CAATTG 1 cut(s) 72
MvaI CCWGG 2 cut(s) 611, 788
MvnI CGCG 5 cut(s) 686, 799, 935, 1019, 1031
MwoI GCNNNNNNNGC 9 cut(s) 252, 542, 559, 657, 725, 901, 1028, 1067, 1076
NciI CCSGG 1 cut(s) 919
NdeII GATC 4 cut(s) 123, 480, 813, 858
NheI GCTAGC 1 cut(s) 688
NlaIII CATG 4 cut(s) 487, 643, 766, 1055
NlaIV GGNNCC 1 cut(s) 181
NmeAIII GCCGAG 2 cut(s) 153, 930
NmuCI GTSAC 1 cut(s) 643
PaeR7I CTCGAG 1 cut(s) 546
PaqCI CACCTGC 1 cut(s) 702
PceI AGGCCT 1 cut(s) 414
PciSI GCTCTTC 1 cut(s) 968
PfoI TCCNGGA 1 cut(s) 609
PkrI GCNGC 8 cut(s) 179, 535, 801, 897, 900, 903, 981, 1021
Ple19I CGATCG 1 cut(s) 126
PmaCI CACGTG 1 cut(s) 819
PmlI CACGTG 1 cut(s) 819
Ppu21I YACGTR 1 cut(s) 819
Psp124BI GAGCTC 1 cut(s) 774
Psp6I CCWGG 2 cut(s) 609, 786
PspCI CACGTG 1 cut(s) 819
PspEI GGTNACC 1 cut(s) 643
PspFI CCCAGC 1 cut(s) 355
PspGI CCWGG 2 cut(s) 609, 786
PspN4I GGNNCC 1 cut(s) 181
PspPI GGNCC 3 cut(s) 245, 801, 920
PspXI VCTCGAGB 1 cut(s) 546
PstI CTGCAG 1 cut(s) 900
PvuI CGATCG 1 cut(s) 126
PvuII CAGCTG 1 cut(s) 901
RsaI GTAC 1 cut(s) 1016
RsaNI GTAC 1 cut(s) 1015
SacI GAGCTC 1 cut(s) 774
SapI GCTCTTC 1 cut(s) 968
SaqAI TTAA 3 cut(s) 395, 408, 435
SatI GCNGC 8 cut(s) 178, 534, 800, 896, 899, 902, 980, 1020
Sau3AI GATC 4 cut(s) 123, 480, 813, 858
Sau96I GGNCC 3 cut(s) 245, 801, 920
ScrFI CCNGG 3 cut(s) 611, 788, 919
SduI GDGCHC 3 cut(s) 459, 576, 774
SfaNI GCATC 1 cut(s) 660
SfcI CTRYAG 2 cut(s) 896, 1065
Sfr274I CTCGAG 1 cut(s) 546
SlaI CTCGAG 1 cut(s) 546
SmlI CTYRAG 2 cut(s) 546, 569
SmoI CTYRAG 2 cut(s) 546, 569
Sse9I AATT 4 cut(s) 72, 117, 312, 578
SseBI AGGCCT 1 cut(s) 414
SspMI CTAG 2 cut(s) 524, 689
SstI GAGCTC 1 cut(s) 774
StuI AGGCCT 1 cut(s) 414
StyD4I CCNGG 3 cut(s) 609, 786, 917
StyI CCWWGG 1 cut(s) 510
TaaI ACNGT 3 cut(s) 667, 931, 952
TaiI ACGT 4 cut(s) 542, 746, 821, 999
TaqI TCGA 3 cut(s) 266, 547, 582
TasI AATT 4 cut(s) 72, 117, 312, 578
TauI GCSGC 4 cut(s) 180, 536, 802, 1022
Tru1I TTAA 3 cut(s) 395, 408, 435
Tru9I TTAA 3 cut(s) 395, 408, 435
TscAI CASTG 1 cut(s) 918
TseFI GTSAC 1 cut(s) 643
TseI GCWGC 4 cut(s) 895, 898, 901, 979
Tsp45I GTSAC 1 cut(s) 643
TspDTI ATGAA 2 cut(s) 184, 452
TspGWI ACGGA 2 cut(s) 269, 901
TspRI CASTG 1 cut(s) 918
XapI RAATTY 1 cut(s) 312
XcmI CCANNNNNNNNNTGG 1 cut(s) 474
XhoI CTCGAG 1 cut(s) 546
XspI CTAG 2 cut(s) 524, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.