Rorug01G0022200

acyl-CoA--sterol O-acyltransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002564
Physical Location & Seq
Reverse (-)
3748313 .. 3768274
19962 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0022200.1

Sequence Viewer

Length: 972 bp
ATGAGCTGCGGACAAAGCAAGGTTGTGTGTGTGACAGGAGCATCTGGGTTCATAGCATCATGGCTAGTTAAGCTCTTATTGCAACGAGGTTATACCGTCAAAGCCAGTGTTCGGGACCCAAATGATCAAAAGAAAACAGAACACCTACTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGACGAAGGTTCTTTTGACCCTGTCGTTGATGGGTGTGAAGGTGTTTTTCATACAGCATCCCCTGCCCTACTCTCATCTACTAATCCGCAGGCAGAATTAATTGAGCCTGCTTTGAAGGGAACGCTTAATGTCCTTGGATCGTGTGTGAAGGTTCAGTCTATCAAAAGGGTGGTTATAACATCCTCTATGGCAGCAGTTGGATTTAATGGAAAACCTCTTGCTCCTGATGTAATAATCGATGAATCTTGGTTTTCAGATCCTACTTTTTGTGAAAAAACAAAGCTTTGGTATATGCTTTCAAAGACATTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAACGAAAAAGGAATTGATATTATTACAATAAATCCGGGATGGGTGATCGGCCCTCTCTTACAGCCAACTCTGAACTTGAGTGTGGAACTAGTTCTGAAACTCGTAAATGGGACCGAAAAGTTTCCCAACAAAACTTACAGACTTGTTGATGTTAGAGATGTTGGTAATGCACATATTCTAGCCTTTGAAAACCCATCAGCTAGTGGACGTTATTGTTTAGTTGGAAGCGTAAAACACTGTTCAGAGGTTGTGAAACTGTTGTACGAGATCTCCCCTGCTCTCAATCTTCCAGATAAATGTGCAGATGACAAGCTTTTCACACCAACTTACCAGGTATCCAAGGAAAGAACCCAAACTTTGGGTGTAAAGTATACTCCGCTTGAAGTGTCTCTGAAGGATACTGTTGAAAGTTTGAAGAACAAGAACTTCTTCTAA

Protein Analysis

323

Amino Acids

35.42

Weight (kDa)

6.95

Isoelectric Point (pI)

26.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 101 8e-09 NmrA-like family
Epimerase PF01370 9 - 247 9.7e-24 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 242 3.2e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 134 9.2e-12 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 196 2.7e-11 Male sterility protein
NAD_binding_10 PF13460 13 - 133 1.6e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 377
AccB7I CCANNNNNTGG 1 cut(s) 895
AccI GTMKAC 1 cut(s) 908
AciI CCGC 3 cut(s) 9, 287, 914
AclWI GGATC 2 cut(s) 346, 452
AcuI CTGAAG 1 cut(s) 950
AfaI GTAC 1 cut(s) 800
AfiI CCNNNNNNNGG 2 cut(s) 111, 895
AgsI TTSAA 7 cut(s) 187, 316, 501, 725, 920, 944, 952
AhlI ACTAGT 1 cut(s) 625
AjnI CCWGG 1 cut(s) 867
AluBI AGCT 7 cut(s) 6, 73, 484, 512, 521, 737, 850
AluI AGCT 7 cut(s) 6, 73, 484, 512, 521, 737, 850
Alw26I GTCTC 1 cut(s) 930
AlwI GGATC 2 cut(s) 346, 452
AoxI GGCC 1 cut(s) 586
ApeKI GCWGC 3 cut(s) 6, 392, 521
AseI ATTAAT 1 cut(s) 299
Asp700I GAANNNNTTC 4 cut(s) 174, 627, 657, 965
AspS9I GGNCC 3 cut(s) 115, 587, 648
AsuC2I CCSGG 1 cut(s) 573
AsuHPI GGTGA 1 cut(s) 592
AvaII GGWCC 2 cut(s) 115, 648
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 2 cut(s) 404, 508
BccI CCATC 4 cut(s) 152, 224, 570, 739
BciT130I CCWGG 1 cut(s) 869
BciVI GTATCC 2 cut(s) 883, 928
BclI TGATCA 1 cut(s) 124
BcnI CCSGG 1 cut(s) 573
BcoDI GTCTC 1 cut(s) 930
BcuI ACTAGT 1 cut(s) 625
BfaI CTAG 4 cut(s) 65, 626, 716, 738
BfuI GTATCC 2 cut(s) 883, 928
BglII AGATCT 1 cut(s) 804
BisI GCNGC 3 cut(s) 7, 393, 522
BlsI GCNGC 3 cut(s) 8, 394, 523
Bme1390I CCNGG 2 cut(s) 573, 869
Bme18I GGWCC 2 cut(s) 115, 648
BmgT120I GGNCC 3 cut(s) 115, 587, 648
BmiI GGNNCC 3 cut(s) 116, 117, 649
BmrFI CCNGG 2 cut(s) 573, 869
BmsI GCATC 3 cut(s) 50, 65, 266
Bpu10I CCTNAGC 1 cut(s) 513
BpuEI CTTGAG 1 cut(s) 634
BpuMI CCSGG 1 cut(s) 573
Bsa29I ATCGAT 1 cut(s) 438
BsaJI CCNNGG 2 cut(s) 334, 876
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 895
Bse1I ACTGG 1 cut(s) 105
BseBI CCWGG 1 cut(s) 869
BseCI ATCGAT 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 334, 876
BseGI GGATG 3 cut(s) 257, 380, 581
BseLI CCNNNNNNNGG 2 cut(s) 111, 895
BseMII CTCAG 1 cut(s) 504
BseNI ACTGG 1 cut(s) 105
BseXI GCAGC 2 cut(s) 404, 508
BsgI GTGCAG 1 cut(s) 858
BshFI GGCC 1 cut(s) 588
BshVI ATCGAT 1 cut(s) 438
BsiSI CCGG 1 cut(s) 572
BslFI GGGAC 2 cut(s) 128, 661
BslI CCNNNNNNNGG 2 cut(s) 111, 895
BsmAI GTCTC 1 cut(s) 930
BsmFI GGGAC 2 cut(s) 128, 661
BsnI GGCC 1 cut(s) 588
Bsp143I GATC 5 cut(s) 124, 338, 457, 582, 804
BspACI CCGC 3 cut(s) 9, 287, 914
BspANI GGCC 1 cut(s) 588
BspCNI CTCAG 1 cut(s) 505
BspDI ATCGAT 1 cut(s) 438
BspLI GGNNCC 3 cut(s) 116, 117, 649
BspPI GGATC 2 cut(s) 346, 452
BsrI ACTGG 1 cut(s) 105
BssECI CCNNGG 2 cut(s) 334, 876
BssMI GATC 5 cut(s) 124, 338, 457, 582, 804
BssNAI GTATAC 1 cut(s) 909
BssT1I CCWWGG 2 cut(s) 334, 876
Bst1107I GTATAC 1 cut(s) 909
Bst2UI CCWGG 1 cut(s) 869
Bst4CI ACNGT 4 cut(s) 97, 776, 795, 940
BstAPI GCANNNNNTGC 1 cut(s) 263
BstC8I GCNNGC 2 cut(s) 291, 309
BstDEI CTNAG 1 cut(s) 513
BstF5I GGATG 3 cut(s) 257, 380, 581
BstKTI GATC 5 cut(s) 127, 341, 460, 585, 807
BstMAI GTCTC 1 cut(s) 930
BstMBI GATC 5 cut(s) 124, 338, 457, 582, 804
BstMWI GCNNNNNNNGC 5 cut(s) 15, 70, 79, 263, 518
BstNI CCWGG 1 cut(s) 869
BstSCI CCNGG 2 cut(s) 571, 867
BstV1I GCAGC 2 cut(s) 404, 508
BstX2I RGATCY 2 cut(s) 457, 804
BstYI RGATCY 2 cut(s) 457, 804
BstZ17I GTATAC 1 cut(s) 909
Bsu15I ATCGAT 1 cut(s) 438
BsuI GTATCC 2 cut(s) 883, 928
BsuRI GGCC 1 cut(s) 588
BsuTUI ATCGAT 1 cut(s) 438
BtsCI GGATG 3 cut(s) 257, 380, 581
BtsIMutI CAGTG 2 cut(s) 112, 772
Cac8I GCNNGC 2 cut(s) 291, 309
Cfr13I GGNCC 3 cut(s) 115, 587, 648
ClaI ATCGAT 1 cut(s) 438
CsiI ACCWGGT 1 cut(s) 867
Csp6I GTAC 1 cut(s) 799
CviAII CATG 1 cut(s) 60
CviQI GTAC 1 cut(s) 799
DdeI CTNAG 1 cut(s) 513
DpnI GATC 5 cut(s) 126, 340, 459, 584, 806
DpnII GATC 5 cut(s) 124, 338, 457, 582, 804
Eco130I CCWWGG 2 cut(s) 334, 876
Eco47I GGWCC 2 cut(s) 115, 648
Eco57I CTGAAG 1 cut(s) 950
EcoO109I RGGNCCY 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 867
EcoT14I CCWWGG 2 cut(s) 334, 876
ErhI CCWWGG 2 cut(s) 334, 876
FaeI CATG 1 cut(s) 63
FalI AAGNNNNNCTT 1 cut(s) 950
FaqI GGGAC 2 cut(s) 128, 661
FatI CATG 1 cut(s) 59
FbaI TGATCA 1 cut(s) 124
FblI GTMKAC 1 cut(s) 908
Fnu4HI GCNGC 3 cut(s) 7, 393, 522
FokI GGATG 3 cut(s) 244, 367, 588
Fsp4HI GCNGC 3 cut(s) 7, 393, 522
FspBI CTAG 4 cut(s) 65, 626, 716, 738
GluI GCNGC 3 cut(s) 7, 393, 522
HaeIII GGCC 1 cut(s) 588
HapII CCGG 1 cut(s) 572
Hin1II CATG 1 cut(s) 63
HindIII AAGCTT 2 cut(s) 482, 848
HinfI GANTC 1 cut(s) 443
HpaII CCGG 1 cut(s) 572
HphI GGTGA 1 cut(s) 592
Hpy166II GTNNAC 2 cut(s) 743, 909
Hpy188I TCNGA 5 cut(s) 457, 609, 633, 781, 930
Hpy188III TCNNGA 3 cut(s) 113, 425, 827
Hpy8I GTNNAC 2 cut(s) 743, 909
HpyAV CCTTC 5 cut(s) 200, 233, 310, 343, 925
HpyCH4III ACNGT 4 cut(s) 97, 776, 795, 940
HpyCH4IV ACGT 1 cut(s) 745
HpyCH4V TGCA 4 cut(s) 82, 536, 707, 839
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 70, 79, 263, 518
HpyF3I CTNAG 1 cut(s) 513
HpySE526I ACGT 1 cut(s) 745
Hsp92II CATG 1 cut(s) 63
KflI GGGWCCC 1 cut(s) 115
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 5 cut(s) 124, 338, 457, 582, 804
LmnI GCTCC 3 cut(s) 38, 161, 427
Lsp1109I GCAGC 2 cut(s) 404, 508
LweI GCATC 3 cut(s) 50, 65, 266
MabI ACCWGGT 1 cut(s) 867
MaeI CTAG 4 cut(s) 65, 626, 716, 738
MaeII ACGT 1 cut(s) 745
MaeIII GTNAC 1 cut(s) 31
MalI GATC 5 cut(s) 126, 340, 459, 584, 806
MboI GATC 5 cut(s) 124, 338, 457, 582, 804
MboII GAAGA 3 cut(s) 815, 958, 964
MflI RGATCY 2 cut(s) 457, 804
MluCI AATT 3 cut(s) 296, 300, 549
MmeI TCCRAC 2 cut(s) 379, 739
MnlI CCTC 6 cut(s) 80, 394, 426, 508, 600, 775
MroXI GAANNNNTTC 4 cut(s) 174, 627, 657, 965
MseI TTAA 4 cut(s) 69, 299, 327, 405
MspI CCGG 1 cut(s) 572
MspR9I CCNGG 2 cut(s) 573, 869
MvaI CCWGG 1 cut(s) 869
MwoI GCNNNNNNNGC 5 cut(s) 15, 70, 79, 263, 518
NciI CCSGG 1 cut(s) 573
NdeII GATC 5 cut(s) 124, 338, 457, 582, 804
NlaIII CATG 1 cut(s) 63
NlaIV GGNNCC 3 cut(s) 116, 117, 649
NmuCI GTSAC 1 cut(s) 31
PdmI GAANNNNTTC 4 cut(s) 174, 627, 657, 965
PfeI GAWTC 1 cut(s) 443
PflFI GACNNNGTC 1 cut(s) 221
PflMI CCANNNNNTGG 1 cut(s) 895
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 3 cut(s) 8, 394, 523
PpuMI RGGWCCY 1 cut(s) 115
PshBI ATTAAT 1 cut(s) 299
PsiI TTATAA 1 cut(s) 377
Psp5II RGGWCCY 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 867
PspGI CCWGG 1 cut(s) 867
PspN4I GGNNCC 3 cut(s) 116, 117, 649
PspPI GGNCC 3 cut(s) 115, 587, 648
PspPPI RGGWCCY 1 cut(s) 115
PsuI RGATCY 2 cut(s) 457, 804
PsyI GACNNNGTC 1 cut(s) 221
RsaI GTAC 1 cut(s) 800
RsaNI GTAC 1 cut(s) 799
SaqAI TTAA 4 cut(s) 69, 299, 327, 405
SatI GCNGC 3 cut(s) 7, 393, 522
Sau3AI GATC 5 cut(s) 124, 338, 457, 582, 804
Sau96I GGNCC 3 cut(s) 115, 587, 648
ScrFI CCNGG 2 cut(s) 573, 869
SexAI ACCWGGT 1 cut(s) 867
SfaNI GCATC 3 cut(s) 50, 65, 266
SinI GGWCC 2 cut(s) 115, 648
SmlI CTYRAG 1 cut(s) 613
SmoI CTYRAG 1 cut(s) 613
SpeI ACTAGT 1 cut(s) 625
Sse9I AATT 3 cut(s) 296, 300, 549
SsiI CCGC 3 cut(s) 9, 287, 914
SspMI CTAG 4 cut(s) 65, 626, 716, 738
StyD4I CCNGG 2 cut(s) 571, 867
StyI CCWWGG 2 cut(s) 334, 876
TaaI ACNGT 4 cut(s) 97, 776, 795, 940
TaiI ACGT 1 cut(s) 748
TaqI TCGA 1 cut(s) 438
TaqII GACCGA 1 cut(s) 665
TasI AATT 3 cut(s) 296, 300, 549
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 4 cut(s) 69, 299, 327, 405
Tru9I TTAA 4 cut(s) 69, 299, 327, 405
TscAI CASTG 2 cut(s) 112, 779
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 3 cut(s) 6, 392, 521
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 4 cut(s) 40, 167, 239, 456
TspRI CASTG 2 cut(s) 112, 779
Tth111I GACNNNGTC 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 895
VpaK11BI GGWCC 2 cut(s) 115, 648
VspI ATTAAT 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 908
XmnI GAANNNNTTC 4 cut(s) 174, 627, 657, 965
XspI CTAG 4 cut(s) 65, 626, 716, 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.