RLG00000030935

acyl-CoA--sterol O-acyltransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002564
Physical Location & Seq
Forward (+)
758323 .. 759428
1106 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030935

Sequence Viewer

Length: 1059 bp
ATGGAGCTAGGTGAGATGGGAAACTTCATCAAGGTATGGCTCTTAGTTTTCTTATCTCTATGCTACTGCTATGTATTACCAAAGTCTGTGCCAAAAGGAGTTCCAAGACTCTTGTGTATACTTCCAATTGTGTGCCTCTTTCTAGACCTTCCTCTTCACTTGGCCTCCATACATCTTGGAGGCACCACTGCTTTCTTCATTGCTTGGCTTGCCAACTTCAAGCTCTTGCTTTTCGCTTTCGGAAAAGGCCCTTTATCTTCCGACCCTGCAATCTCTCTTCCCCGTTTCATCGCCGTTGCTTGCTTGCCGATCAAGATCCAACAAAACCCAGTTCCAAAATCCAATGACCAAAATGGCCACAACAAAGAAAAGCCATTTCCAGGAATGCCCAAAAAGGGCCAAAAATCCCTCGTAAATTACGCAACCAAGGGCCTGCTTTTGGGTTTGTTGGTACGAGTATATAACTACAGTGAGCATATTCATCCAAAGCTCCTATGGGTCATTTGCAATTGTGGTTCGACTTGTCATGGACTCGAACTCGAGCCACAGTTCAATGAACCATACCTTGCATCTTCGCTCCAAGACTTTTGGGGCCGAAGGTGGAACATCATGGTCACCAGCATTCTCCGCCCCACCGTATACGAGCCTGTTCTCACAGTGGCAACACGTGTAGCGGGGCGCAAATGGGCCCCTCTGCCGGCCGTAATGGGGACTTTTGTGGTGTCGGCAATCATGCACGAGCTCATATTCTACTACCTTGGGCGTACGAGTCCCACATGGGAAGTTACCTGGTTCTTTCTCCTCCACGGGGTGTGTTTGGTGGTTGAGATCGCCGTAAAGAAGAAGGCAGTTGCTTCACGAAGGTTCCAGTTGCCTAGGCTGATATCGGGACCGTTGACGGTTGCATTTGTGACGGCCACCGTGTTCTGGCTCTTCTTGCCTGCACTGCTCCGGTGTAAGGCGGATGTGAGGGCGTTGGAGGAGTACTCCGCCGCCGGCACATTTGTGAAGAATGTAGGTCGTGCTTTCATTTCTAACGTGATGAAATCCACGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT_2

Protein Analysis

353

Amino Acids

39.34

Weight (kDa)

9.61

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT_2 PF13813 181 - 267 2.1e-18 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 182
AccI GTMKAC 2 cut(s) 118, 639
AciI CCGC 5 cut(s) 628, 674, 962, 990, 993
AclWI GGATC 1 cut(s) 310
AcoI YGGCCR 3 cut(s) 355, 699, 915
AcvI CACGTG 1 cut(s) 668
AdeI CACNNNGTG 1 cut(s) 811
AfaI GTAC 3 cut(s) 453, 766, 986
AfiI CCNNNNNNNGG 8 cut(s) 380, 395, 439, 697, 708, 808, 927, 958
AflIII ACRYGT 2 cut(s) 665, 667
AgsI TTSAA 2 cut(s) 220, 553
AjnI CCWGG 2 cut(s) 379, 788
AleI CACNNNNGTG 1 cut(s) 1004
AluBI AGCT 4 cut(s) 7, 223, 490, 742
AluI AGCT 4 cut(s) 7, 223, 490, 742
Alw21I GWGCWC 1 cut(s) 744
AlwI GGATC 1 cut(s) 310
Ama87I CYCGRG 1 cut(s) 539
AoxI GGCC 9 cut(s) 162, 247, 355, 397, 430, 592, 687, 699, 915
ApaI GGGCCC 1 cut(s) 691
AspA2I CCTAGG 1 cut(s) 875
AspLEI GCGC 1 cut(s) 681
AspS9I GGNCC 7 cut(s) 248, 397, 430, 592, 687, 688, 890
AsuHPI GGTGA 2 cut(s) 23, 607
AvaI CYCGRG 1 cut(s) 539
AvaII GGWCC 1 cut(s) 890
AvrII CCTAGG 1 cut(s) 875
BaeGI GKGCMC 1 cut(s) 691
BalI TGGCCA 1 cut(s) 357
BanI GGYRCC 1 cut(s) 182
BanII GRGCYC 2 cut(s) 691, 744
BauI CACGAG 1 cut(s) 737
BbrPI CACGTG 1 cut(s) 668
Bbv12I GWGCWC 1 cut(s) 744
BccI CCATC 1 cut(s) 10
BceAI ACGGC 4 cut(s) 278, 686, 818, 930
BciT130I CCWGG 2 cut(s) 381, 790
BfaI CTAG 3 cut(s) 8, 143, 876
BfmI CTRYAG 1 cut(s) 466
BisI GCNGC 1 cut(s) 993
BlnI CCTAGG 1 cut(s) 875
BlsI GCNGC 1 cut(s) 994
BmcAI AGTACT 1 cut(s) 986
Bme1390I CCNGG 2 cut(s) 381, 790
Bme18I GGWCC 1 cut(s) 890
BmeT110I CYCGRG 1 cut(s) 539
BmgT120I GGNCC 7 cut(s) 248, 397, 430, 592, 687, 688, 890
BmiI GGNNCC 6 cut(s) 184, 593, 689, 690, 866, 891
BmrFI CCNGG 2 cut(s) 381, 790
BmrI ACTGGG 1 cut(s) 323
BmsI GCATC 1 cut(s) 578
BmuI ACTGGG 1 cut(s) 323
BplI GAGNNNNNCTC 2 cut(s) 971, 1003
BsaAI YACGTR 1 cut(s) 668
BsaBI GATNNNNATC 1 cut(s) 314
BsaJI CCNNGG 4 cut(s) 426, 757, 805, 875
BsaWI WCCGGW 1 cut(s) 951
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 8 cut(s) 380, 395, 439, 697, 708, 808, 927, 958
Bse118I RCCGGY 2 cut(s) 697, 995
Bse1I ACTGG 2 cut(s) 329, 868
Bse3DI GCAATG 1 cut(s) 198
Bse8I GATNNNNATC 1 cut(s) 314
BseBI CCWGG 2 cut(s) 381, 790
BseDI CCNNGG 4 cut(s) 426, 757, 805, 875
BseGI GGATG 2 cut(s) 481, 970
BseJI GATNNNNATC 1 cut(s) 314
BseLI CCNNNNNNNGG 8 cut(s) 380, 395, 439, 697, 708, 808, 927, 958
BseMI GCAATG 1 cut(s) 198
BseNI ACTGG 2 cut(s) 329, 868
BseRI GAGGAG 2 cut(s) 791, 995
BseSI GKGCMC 1 cut(s) 691
BseX3I CGGCCG 1 cut(s) 699
BsgI GTGCAG 1 cut(s) 927
Bsh1285I CGRYCG 1 cut(s) 702
BshFI GGCC 9 cut(s) 164, 249, 357, 399, 432, 594, 689, 701, 917
BshNI GGYRCC 1 cut(s) 182
BsiEI CGRYCG 1 cut(s) 702
BsiHKAI GWGCWC 1 cut(s) 744
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 3 cut(s) 698, 952, 996
BsiWI CGTACG 1 cut(s) 764
BslFI GGGAC 3 cut(s) 724, 756, 903
BslI CCNNNNNNNGG 8 cut(s) 380, 395, 439, 697, 708, 808, 927, 958
BsmFI GGGAC 3 cut(s) 724, 756, 903
BsmI GAATGC 2 cut(s) 390, 621
BsnI GGCC 9 cut(s) 164, 249, 357, 399, 432, 594, 689, 701, 917
BsoBI CYCGRG 1 cut(s) 539
Bsp120I GGGCCC 1 cut(s) 687
Bsp1286I GDGCHC 2 cut(s) 691, 744
Bsp143I GATC 3 cut(s) 309, 315, 828
BspACI CCGC 5 cut(s) 628, 674, 962, 990, 993
BspANI GGCC 9 cut(s) 164, 249, 357, 399, 432, 594, 689, 701, 917
BspLI GGNNCC 6 cut(s) 184, 593, 689, 690, 866, 891
BspPI GGATC 1 cut(s) 310
BspQI GCTCTTC 1 cut(s) 938
BspT107I GGYRCC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 198
BsrFI RCCGGY 2 cut(s) 697, 995
BsrI ACTGG 2 cut(s) 329, 868
BssAI RCCGGY 2 cut(s) 697, 995
BssECI CCNNGG 4 cut(s) 426, 757, 805, 875
BssMI GATC 3 cut(s) 309, 315, 828
BssNAI GTATAC 2 cut(s) 119, 640
BssSI CACGAG 1 cut(s) 737
BssT1I CCWWGG 3 cut(s) 426, 757, 875
Bst1107I GTATAC 2 cut(s) 119, 640
Bst2BI CACGAG 1 cut(s) 737
Bst2UI CCWGG 2 cut(s) 381, 790
Bst4CI ACNGT 7 cut(s) 470, 549, 637, 658, 894, 901, 922
Bst6I CTCTTC 3 cut(s) 159, 282, 938
BstBAI YACGTR 1 cut(s) 668
BstC8I GCNNGC 7 cut(s) 210, 301, 305, 434, 699, 942, 997
BstDEI CTNAG 1 cut(s) 43
BstDSI CCRYGG 1 cut(s) 805
BstEII GGTNACC 1 cut(s) 613
BstF5I GGATG 2 cut(s) 481, 970
BstHHI GCGC 1 cut(s) 681
BstKTI GATC 3 cut(s) 312, 318, 831
BstMBI GATC 3 cut(s) 309, 315, 828
BstMCI CGRYCG 1 cut(s) 702
BstMWI GCNNNNNNNGC 4 cut(s) 209, 627, 937, 946
BstNI CCWGG 2 cut(s) 381, 790
BstPI GGTNACC 1 cut(s) 613
BstSCI CCNGG 2 cut(s) 379, 788
BstSFI CTRYAG 1 cut(s) 466
BstSLI GKGCMC 1 cut(s) 691
BstX2I RGATCY 1 cut(s) 315
BstYI RGATCY 1 cut(s) 315
BstZ17I GTATAC 2 cut(s) 119, 640
BstZI CGGCCG 1 cut(s) 699
BsuRI GGCC 9 cut(s) 164, 249, 357, 399, 432, 594, 689, 701, 917
BtgI CCRYGG 1 cut(s) 805
BtgZI GCGATG 1 cut(s) 274
BtsCI GGATG 2 cut(s) 481, 970
BtsI GCAGTG 2 cut(s) 186, 944
BtsIMutI CAGTG 4 cut(s) 186, 475, 663, 944
Cac8I GCNNGC 7 cut(s) 210, 301, 305, 434, 699, 942, 997
CfoI GCGC 1 cut(s) 681
Cfr10I RCCGGY 2 cut(s) 697, 995
Cfr13I GGNCC 7 cut(s) 248, 397, 430, 592, 687, 688, 890
CsiI ACCWGGT 1 cut(s) 788
Csp6I GTAC 3 cut(s) 452, 765, 985
CviAII CATG 4 cut(s) 527, 610, 733, 777
CviQI GTAC 3 cut(s) 452, 765, 985
DdeI CTNAG 1 cut(s) 43
DpnI GATC 3 cut(s) 311, 317, 830
DpnII GATC 3 cut(s) 309, 315, 828
DraIII CACNNNGTG 1 cut(s) 811
EaeI YGGCCR 3 cut(s) 355, 699, 915
EagI CGGCCG 1 cut(s) 699
Eam1104I CTCTTC 3 cut(s) 159, 282, 938
EarI CTCTTC 3 cut(s) 159, 282, 938
EciI GGCGGA 3 cut(s) 617, 977, 979
Ecl136II GAGCTC 1 cut(s) 742
EclXI CGGCCG 1 cut(s) 699
Eco130I CCWWGG 3 cut(s) 426, 757, 875
Eco24I GRGCYC 2 cut(s) 691, 744
Eco32I GATATC 1 cut(s) 885
Eco47I GGWCC 1 cut(s) 890
Eco52I CGGCCG 1 cut(s) 699
Eco53kI GAGCTC 1 cut(s) 742
Eco72I CACGTG 1 cut(s) 668
Eco88I CYCGRG 1 cut(s) 539
Eco91I GGTNACC 1 cut(s) 613
EcoICRI GAGCTC 1 cut(s) 742
EcoO109I RGGNCCY 3 cut(s) 248, 430, 688
EcoO65I GGTNACC 1 cut(s) 613
EcoRII CCWGG 2 cut(s) 379, 788
EcoRV GATATC 1 cut(s) 885
EcoT14I CCWWGG 3 cut(s) 426, 757, 875
EcoT38I GRGCYC 2 cut(s) 691, 744
ErhI CCWWGG 3 cut(s) 426, 757, 875
FaeI CATG 4 cut(s) 530, 613, 736, 780
FaqI GGGAC 3 cut(s) 724, 756, 903
FatI CATG 4 cut(s) 526, 609, 732, 776
FauI CCCGC 1 cut(s) 667
FblI GTMKAC 2 cut(s) 118, 639
Fnu4HI GCNGC 1 cut(s) 993
FokI GGATG 2 cut(s) 468, 977
FriOI GRGCYC 2 cut(s) 691, 744
Fsp4HI GCNGC 1 cut(s) 993
FspBI CTAG 3 cut(s) 8, 143, 876
GlaI GCGC 1 cut(s) 680
GluI GCNGC 1 cut(s) 993
HaeIII GGCC 9 cut(s) 164, 249, 357, 399, 432, 594, 689, 701, 917
HapII CCGG 3 cut(s) 698, 952, 996
HhaI GCGC 1 cut(s) 681
Hin1II CATG 4 cut(s) 530, 613, 736, 780
Hin6I GCGC 1 cut(s) 679
HinP1I GCGC 1 cut(s) 679
HincII GTYRAC 1 cut(s) 897
HindII GTYRAC 1 cut(s) 897
HinfI GANTC 3 cut(s) 108, 531, 769
HpaII CCGG 3 cut(s) 698, 952, 996
HphI GGTGA 2 cut(s) 23, 607
Hpy166II GTNNAC 3 cut(s) 119, 640, 897
Hpy188I TCNGA 2 cut(s) 242, 262
Hpy188III TCNNGA 4 cut(s) 143, 313, 858, 888
Hpy8I GTNNAC 3 cut(s) 119, 640, 897
HpyAV CCTTC 4 cut(s) 158, 591, 838, 855
HpyCH4III ACNGT 7 cut(s) 470, 549, 637, 658, 894, 901, 922
HpyCH4IV ACGT 2 cut(s) 667, 1038
HpyCH4V TGCA 6 cut(s) 269, 507, 569, 736, 905, 944
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 627, 937, 946
HpyF3I CTNAG 1 cut(s) 43
HpySE526I ACGT 2 cut(s) 667, 1038
Hsp92II CATG 4 cut(s) 530, 613, 736, 780
HspAI GCGC 1 cut(s) 679
KroI GCCGGC 2 cut(s) 697, 995
KroNI GCCGGC 2 cut(s) 699, 997
Kzo9I GATC 3 cut(s) 309, 315, 828
LguI GCTCTTC 1 cut(s) 938
LmnI GCTCC 4 cut(s) 4, 495, 582, 954
LweI GCATC 1 cut(s) 578
MabI ACCWGGT 1 cut(s) 788
MaeI CTAG 3 cut(s) 8, 143, 876
MaeII ACGT 2 cut(s) 667, 1038
MaeIII GTNAC 3 cut(s) 613, 784, 910
MalI GATC 3 cut(s) 311, 317, 830
MboI GATC 3 cut(s) 309, 315, 828
MboII GAAGA 8 cut(s) 146, 187, 249, 269, 564, 853, 925, 1021
MfeI CAATTG 2 cut(s) 126, 508
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 2 cut(s) 691, 744
MlsI TGGCCA 1 cut(s) 357
MluCI AATT 3 cut(s) 126, 415, 508
MluNI TGGCCA 1 cut(s) 357
MlyI GAGTC 3 cut(s) 102, 525, 778
MmeI TCCRAC 3 cut(s) 285, 343, 957
MnlI CCTC 9 cut(s) 146, 162, 173, 175, 419, 702, 812, 963, 973
Mox20I TGGCCA 1 cut(s) 357
MroNI GCCGGC 2 cut(s) 697, 995
MscI TGGCCA 1 cut(s) 357
MslI CAYNNNNRTG 1 cut(s) 1004
Msp20I TGGCCA 1 cut(s) 357
MspI CCGG 3 cut(s) 698, 952, 996
MspR9I CCNGG 2 cut(s) 381, 790
MunI CAATTG 2 cut(s) 126, 508
Mva1269I GAATGC 2 cut(s) 390, 621
MvaI CCWGG 2 cut(s) 381, 790
MwoI GCNNNNNNNGC 4 cut(s) 209, 627, 937, 946
NaeI GCCGGC 2 cut(s) 699, 997
NdeII GATC 3 cut(s) 309, 315, 828
NgoMIV GCCGGC 2 cut(s) 697, 995
NlaIII CATG 4 cut(s) 530, 613, 736, 780
NlaIV GGNNCC 6 cut(s) 184, 593, 689, 690, 866, 891
NmuCI GTSAC 2 cut(s) 613, 910
OliI CACNNNNGTG 1 cut(s) 1004
PaeR7I CTCGAG 1 cut(s) 539
PciSI GCTCTTC 1 cut(s) 938
PctI GAATGC 2 cut(s) 390, 621
PdiI GCCGGC 2 cut(s) 699, 997
Pfl23II CGTACG 1 cut(s) 764
PfoI TCCNGGA 1 cut(s) 379
PkrI GCNGC 1 cut(s) 994
PleI GAGTC 3 cut(s) 102, 525, 777
PmaCI CACGTG 1 cut(s) 668
PmlI CACGTG 1 cut(s) 668
PpsI GAGTC 3 cut(s) 102, 525, 777
Ppu21I YACGTR 1 cut(s) 668
Psp124BI GAGCTC 1 cut(s) 744
Psp6I CCWGG 2 cut(s) 379, 788
PspCI CACGTG 1 cut(s) 668
PspEI GGTNACC 1 cut(s) 613
PspGI CCWGG 2 cut(s) 379, 788
PspLI CGTACG 1 cut(s) 764
PspN4I GGNNCC 6 cut(s) 184, 593, 689, 690, 866, 891
PspOMI GGGCCC 1 cut(s) 687
PspPI GGNCC 7 cut(s) 248, 397, 430, 592, 687, 688, 890
PspXI VCTCGAGB 1 cut(s) 539
PsuI RGATCY 1 cut(s) 315
RsaI GTAC 3 cut(s) 453, 766, 986
RsaNI GTAC 3 cut(s) 452, 765, 985
RseI CAYNNNNRTG 1 cut(s) 1004
SacI GAGCTC 1 cut(s) 744
SapI GCTCTTC 1 cut(s) 938
SatI GCNGC 1 cut(s) 993
Sau3AI GATC 3 cut(s) 309, 315, 828
Sau96I GGNCC 7 cut(s) 248, 397, 430, 592, 687, 688, 890
ScaI AGTACT 1 cut(s) 986
SchI GAGTC 3 cut(s) 102, 525, 778
ScrFI CCNGG 2 cut(s) 381, 790
SduI GDGCHC 2 cut(s) 691, 744
SexAI ACCWGGT 1 cut(s) 788
SfaNI GCATC 1 cut(s) 578
SfcI CTRYAG 1 cut(s) 466
Sfr274I CTCGAG 1 cut(s) 539
SinI GGWCC 1 cut(s) 890
SlaI CTCGAG 1 cut(s) 539
SmiMI CAYNNNNRTG 1 cut(s) 1004
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 3 cut(s) 126, 415, 508
SsiI CCGC 5 cut(s) 628, 674, 962, 990, 993
SspMI CTAG 3 cut(s) 8, 143, 876
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 2 cut(s) 379, 788
StyI CCWWGG 3 cut(s) 426, 757, 875
TaaI ACNGT 7 cut(s) 470, 549, 637, 658, 894, 901, 922
TaiI ACGT 2 cut(s) 670, 1041
TaqI TCGA 3 cut(s) 518, 534, 540
TasI AATT 3 cut(s) 126, 415, 508
TatI WGTACW 1 cut(s) 984
TauI GCSGC 1 cut(s) 995
TscAI CASTG 4 cut(s) 193, 475, 663, 951
TseFI GTSAC 2 cut(s) 613, 910
Tsp45I GTSAC 2 cut(s) 613, 910
TspDTI ATGAA 7 cut(s) 16, 187, 277, 470, 570, 1018, 1058
TspRI CASTG 4 cut(s) 193, 475, 663, 951
VpaK11BI GGWCC 1 cut(s) 890
XbaI TCTAGA 1 cut(s) 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 492
XhoI CTCGAG 1 cut(s) 539
XmaJI CCTAGG 1 cut(s) 875
XmiI GTMKAC 2 cut(s) 118, 639
XspI CTAG 3 cut(s) 8, 143, 876
ZrmI AGTACT 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.