RchiOBHm_Chr5g0001521

acyl-CoA--sterol O-acyltransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002564
Physical Location & Seq
Forward (+)
792615 .. 794044
1430 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28294

Sequence Viewer

Length: 1110 bp
ATGGTGATGGAGCTAGGTGAGATGGGAAGCTTCATCAGGGTATGGCTCTTTGTTTTCTTATCTCTATGCTACTGCTATGCATTACCAAGGTTTGTGCCAAAAGGAGTTCCAAGACTCTTGTGTATACTTCCAATTGTGTGCCTCTTTCTAGACCTTCCTCTTCACCTGTCCTCCATACATCTTGGAGGCACCACTGCTTTCTTCATTGCTTGGCTTGCCAACTTCAAGCTCTTGCTTTTCGCTTTCGGAAAAGGCCCTTTATCTTCGGACCCTTCAATCTCTCTTCCCCGTTTCATCGCCGTCGCTTGCTTGCCGATCAAGATCCAACAAAGCCCGGTTCCAAAATCCCATGACCAAAATGGCCACAACAAAGAAAAGCCATTTCCAGGAATGCCCAAAAAGGGCCAAAAATCCCTCATAAATTACGCAACCAAGGGCCTACTTTTGGGTTTGTTGGTACGAGTATATAACTACAGTGAGCATATTCATCCAAAGCTCCTATGGGTCATGTATTGCTTCCACATTTATTTTGCATTGGAAATCATCCTGGTTGTAGTTGCAACTGTGGTTCGACTTGTCATGGGACTCGAACTCGAGCCACAGTTCAATGAACCATACCTTGCATCTTCGCTCCAAGACTTTTGGGGCCGAAGGTGGAACATCATGGTCACCAGCATTCTCCGCCCCACCGTATACGAGCCTGTTCTCACAGTGGCAACACGTGTAGTGGGGCGCAAATGGGCCCCTCTACCGGCCGTAATGGGGACTTTTGTGGTGTCGGCAATCATGCACGAGCTCATATTCTACTACCTTGGGCGTATGAGTCCCACATGGGAAGTTACCTGGTTCTTTCTCCTCCACGGGGGGTGTTTGGTGGTTGAGATCGCCGTCAAGAAGGCAGTTGCTTCACGAAGGTTCCAGTTGCCTAGGCTGATATCGGGACCGTTGACGGTTGCATTTGTGACGGCCACCGTGTTCTGGCTCTTCTTGCCTGCACTGCTCCGGTGTAAGGTGGATGTGAGGGCGTTGGAGGAGTACGCCGCCGCCGGCACATTTGTGAAGAATGTAGGTCGTGCTTTCATTTCTAACGTGATGAAATCCACGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT_2

Protein Analysis

369

Amino Acids

41.61

Weight (kDa)

9.66

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT_2 PF13813 199 - 285 2.9e-18 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AccI GTMKAC 2 cut(s) 124, 693
AciI CCGC 3 cut(s) 682, 1041, 1044
AclWI GGATC 1 cut(s) 316
AcoI YGGCCR 3 cut(s) 361, 753, 966
AcvI CACGTG 1 cut(s) 722
AfaI GTAC 2 cut(s) 459, 1037
AfiI CCNNNNNNNGG 8 cut(s) 386, 401, 445, 751, 762, 862, 978, 1009
AflIII ACRYGT 2 cut(s) 719, 721
AgsI TTSAA 3 cut(s) 226, 276, 607
AjnI CCWGG 3 cut(s) 385, 546, 842
AleI CACNNNNGTG 1 cut(s) 1055
AluBI AGCT 5 cut(s) 13, 30, 229, 496, 796
AluI AGCT 5 cut(s) 13, 30, 229, 496, 796
Alw21I GWGCWC 1 cut(s) 798
AlwI GGATC 1 cut(s) 316
Ama87I CYCGRG 1 cut(s) 593
AoxI GGCC 8 cut(s) 253, 361, 403, 436, 646, 741, 753, 966
ApaI GGGCCC 1 cut(s) 745
AspA2I CCTAGG 1 cut(s) 926
AspLEI GCGC 1 cut(s) 735
AspS9I GGNCC 8 cut(s) 254, 268, 403, 436, 646, 741, 742, 941
AsuC2I CCSGG 1 cut(s) 335
AsuHPI GGTGA 4 cut(s) 16, 29, 155, 661
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 2 cut(s) 268, 941
AvrII CCTAGG 1 cut(s) 926
BaeGI GKGCMC 1 cut(s) 745
BalI TGGCCA 1 cut(s) 363
BanI GGYRCC 1 cut(s) 188
BanII GRGCYC 2 cut(s) 745, 798
BauI CACGAG 1 cut(s) 791
BbrPI CACGTG 1 cut(s) 722
Bbv12I GWGCWC 1 cut(s) 798
BccI CCATC 1 cut(s) 16
BceAI ACGGC 4 cut(s) 284, 740, 872, 981
BciT130I CCWGG 3 cut(s) 387, 548, 844
BcnI CCSGG 1 cut(s) 335
BfaI CTAG 3 cut(s) 14, 149, 927
BfmI CTRYAG 1 cut(s) 472
BisI GCNGC 2 cut(s) 1041, 1044
BlnI CCTAGG 1 cut(s) 926
BlsI GCNGC 2 cut(s) 1042, 1045
Bme1390I CCNGG 4 cut(s) 335, 387, 548, 844
Bme18I GGWCC 2 cut(s) 268, 941
BmeT110I CYCGRG 1 cut(s) 593
BmgT120I GGNCC 8 cut(s) 254, 268, 403, 436, 646, 741, 742, 941
BmiI GGNNCC 8 cut(s) 190, 270, 339, 647, 743, 744, 917, 942
BmrFI CCNGG 4 cut(s) 335, 387, 548, 844
BmsI GCATC 1 cut(s) 632
BpuMI CCSGG 1 cut(s) 335
BsaAI YACGTR 1 cut(s) 722
BsaBI GATNNNNATC 1 cut(s) 320
BsaJI CCNNGG 5 cut(s) 86, 432, 811, 859, 926
BsaWI WCCGGW 1 cut(s) 1002
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 8 cut(s) 386, 401, 445, 751, 762, 862, 978, 1009
Bse118I RCCGGY 2 cut(s) 751, 1046
Bse1I ACTGG 1 cut(s) 919
Bse3DI GCAATG 1 cut(s) 204
Bse8I GATNNNNATC 1 cut(s) 320
BseBI CCWGG 3 cut(s) 387, 548, 844
BseDI CCNNGG 5 cut(s) 86, 432, 811, 859, 926
BseGI GGATG 3 cut(s) 487, 543, 1021
BseJI GATNNNNATC 1 cut(s) 320
BseLI CCNNNNNNNGG 8 cut(s) 386, 401, 445, 751, 762, 862, 978, 1009
BseMI GCAATG 1 cut(s) 204
BseNI ACTGG 1 cut(s) 919
BseRI GAGGAG 2 cut(s) 845, 1046
BseSI GKGCMC 1 cut(s) 745
BseX3I CGGCCG 1 cut(s) 753
BsgI GTGCAG 1 cut(s) 978
Bsh1285I CGRYCG 1 cut(s) 756
BshFI GGCC 8 cut(s) 255, 363, 405, 438, 648, 743, 755, 968
BshNI GGYRCC 1 cut(s) 188
BsiEI CGRYCG 1 cut(s) 756
BsiHKAI GWGCWC 1 cut(s) 798
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 4 cut(s) 335, 752, 1003, 1047
BslFI GGGAC 4 cut(s) 597, 778, 810, 954
BslI CCNNNNNNNGG 8 cut(s) 386, 401, 445, 751, 762, 862, 978, 1009
BsmFI GGGAC 4 cut(s) 597, 778, 810, 954
BsmI GAATGC 2 cut(s) 396, 675
BsnI GGCC 8 cut(s) 255, 363, 405, 438, 648, 743, 755, 968
BsoBI CYCGRG 1 cut(s) 593
Bsp120I GGGCCC 1 cut(s) 741
Bsp1286I GDGCHC 2 cut(s) 745, 798
Bsp143I GATC 3 cut(s) 315, 321, 882
BspACI CCGC 3 cut(s) 682, 1041, 1044
BspANI GGCC 8 cut(s) 255, 363, 405, 438, 648, 743, 755, 968
BspLI GGNNCC 8 cut(s) 190, 270, 339, 647, 743, 744, 917, 942
BspPI GGATC 1 cut(s) 316
BspQI GCTCTTC 1 cut(s) 989
BspT107I GGYRCC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 204
BsrFI RCCGGY 2 cut(s) 751, 1046
BsrI ACTGG 1 cut(s) 919
BssAI RCCGGY 2 cut(s) 751, 1046
BssECI CCNNGG 5 cut(s) 86, 432, 811, 859, 926
BssMI GATC 3 cut(s) 315, 321, 882
BssNAI GTATAC 2 cut(s) 125, 694
BssSI CACGAG 1 cut(s) 791
BssT1I CCWWGG 4 cut(s) 86, 432, 811, 926
Bst1107I GTATAC 2 cut(s) 125, 694
Bst2BI CACGAG 1 cut(s) 791
Bst2UI CCWGG 3 cut(s) 387, 548, 844
Bst4CI ACNGT 8 cut(s) 476, 565, 603, 691, 712, 945, 952, 973
Bst6I CTCTTC 3 cut(s) 165, 288, 989
BstBAI YACGTR 1 cut(s) 722
BstC8I GCNNGC 5 cut(s) 216, 307, 311, 993, 1048
BstDSI CCRYGG 1 cut(s) 859
BstEII GGTNACC 1 cut(s) 667
BstF5I GGATG 3 cut(s) 487, 543, 1021
BstHHI GCGC 1 cut(s) 735
BstKTI GATC 3 cut(s) 318, 324, 885
BstMBI GATC 3 cut(s) 315, 321, 882
BstMCI CGRYCG 1 cut(s) 756
BstMWI GCNNNNNNNGC 4 cut(s) 215, 681, 988, 997
BstNI CCWGG 3 cut(s) 387, 548, 844
BstPI GGTNACC 1 cut(s) 667
BstSCI CCNGG 4 cut(s) 333, 385, 546, 842
BstSFI CTRYAG 1 cut(s) 472
BstSLI GKGCMC 1 cut(s) 745
BstX2I RGATCY 1 cut(s) 321
BstYI RGATCY 1 cut(s) 321
BstZ17I GTATAC 2 cut(s) 125, 694
BstZI CGGCCG 1 cut(s) 753
BsuRI GGCC 8 cut(s) 255, 363, 405, 438, 648, 743, 755, 968
BtgI CCRYGG 1 cut(s) 859
BtgZI GCGATG 1 cut(s) 280
BtsCI GGATG 3 cut(s) 487, 543, 1021
BtsI GCAGTG 2 cut(s) 192, 995
BtsIMutI CAGTG 4 cut(s) 192, 481, 717, 995
Cac8I GCNNGC 5 cut(s) 216, 307, 311, 993, 1048
CfoI GCGC 1 cut(s) 735
Cfr10I RCCGGY 2 cut(s) 751, 1046
Cfr13I GGNCC 8 cut(s) 254, 268, 403, 436, 646, 741, 742, 941
CsiI ACCWGGT 1 cut(s) 842
Csp6I GTAC 2 cut(s) 458, 1036
CviAII CATG 6 cut(s) 350, 508, 580, 664, 787, 831
CviQI GTAC 2 cut(s) 458, 1036
DpnI GATC 3 cut(s) 317, 323, 884
DpnII GATC 3 cut(s) 315, 321, 882
EaeI YGGCCR 3 cut(s) 361, 753, 966
EagI CGGCCG 1 cut(s) 753
Eam1104I CTCTTC 3 cut(s) 165, 288, 989
EarI CTCTTC 3 cut(s) 165, 288, 989
EciI GGCGGA 1 cut(s) 671
Ecl136II GAGCTC 1 cut(s) 796
EclXI CGGCCG 1 cut(s) 753
Eco130I CCWWGG 4 cut(s) 86, 432, 811, 926
Eco24I GRGCYC 2 cut(s) 745, 798
Eco32I GATATC 1 cut(s) 936
Eco47I GGWCC 2 cut(s) 268, 941
Eco52I CGGCCG 1 cut(s) 753
Eco53kI GAGCTC 1 cut(s) 796
Eco72I CACGTG 1 cut(s) 722
Eco88I CYCGRG 1 cut(s) 593
Eco91I GGTNACC 1 cut(s) 667
EcoICRI GAGCTC 1 cut(s) 796
EcoO109I RGGNCCY 3 cut(s) 254, 436, 742
EcoO65I GGTNACC 1 cut(s) 667
EcoRII CCWGG 3 cut(s) 385, 546, 842
EcoRV GATATC 1 cut(s) 936
EcoT14I CCWWGG 4 cut(s) 86, 432, 811, 926
EcoT22I ATGCAT 1 cut(s) 82
EcoT38I GRGCYC 2 cut(s) 745, 798
ErhI CCWWGG 4 cut(s) 86, 432, 811, 926
FaeI CATG 6 cut(s) 353, 511, 583, 667, 790, 834
FaqI GGGAC 4 cut(s) 597, 778, 810, 954
FatI CATG 6 cut(s) 349, 507, 579, 663, 786, 830
FblI GTMKAC 2 cut(s) 124, 693
Fnu4HI GCNGC 2 cut(s) 1041, 1044
FokI GGATG 3 cut(s) 474, 530, 1028
FriOI GRGCYC 2 cut(s) 745, 798
Fsp4HI GCNGC 2 cut(s) 1041, 1044
FspBI CTAG 3 cut(s) 14, 149, 927
GlaI GCGC 1 cut(s) 734
GluI GCNGC 2 cut(s) 1041, 1044
HaeIII GGCC 8 cut(s) 255, 363, 405, 438, 648, 743, 755, 968
HapII CCGG 4 cut(s) 335, 752, 1003, 1047
HhaI GCGC 1 cut(s) 735
Hin1II CATG 6 cut(s) 353, 511, 583, 667, 790, 834
Hin6I GCGC 1 cut(s) 733
HinP1I GCGC 1 cut(s) 733
HincII GTYRAC 1 cut(s) 948
HindII GTYRAC 1 cut(s) 948
HindIII AAGCTT 1 cut(s) 28
HinfI GANTC 3 cut(s) 114, 585, 823
HpaII CCGG 4 cut(s) 335, 752, 1003, 1047
HphI GGTGA 4 cut(s) 16, 29, 155, 661
Hpy166II GTNNAC 3 cut(s) 125, 694, 948
Hpy188I TCNGA 2 cut(s) 248, 268
Hpy188III TCNNGA 5 cut(s) 149, 319, 892, 909, 939
Hpy8I GTNNAC 3 cut(s) 125, 694, 948
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 5 cut(s) 164, 282, 645, 889, 906
HpyCH4III ACNGT 8 cut(s) 476, 565, 603, 691, 712, 945, 952, 973
HpyCH4IV ACGT 2 cut(s) 721, 1089
HpyCH4V TGCA 7 cut(s) 80, 533, 560, 623, 790, 956, 995
HpyF10VI GCNNNNNNNGC 4 cut(s) 215, 681, 988, 997
HpySE526I ACGT 2 cut(s) 721, 1089
Hsp92II CATG 6 cut(s) 353, 511, 583, 667, 790, 834
HspAI GCGC 1 cut(s) 733
KroI GCCGGC 1 cut(s) 1046
KroNI GCCGGC 1 cut(s) 1048
Kzo9I GATC 3 cut(s) 315, 321, 882
LguI GCTCTTC 1 cut(s) 989
LmnI GCTCC 4 cut(s) 10, 501, 636, 1005
LweI GCATC 1 cut(s) 632
MabI ACCWGGT 1 cut(s) 842
MaeI CTAG 3 cut(s) 14, 149, 927
MaeII ACGT 2 cut(s) 721, 1089
MaeIII GTNAC 3 cut(s) 667, 838, 961
MalI GATC 3 cut(s) 317, 323, 884
MboI GATC 3 cut(s) 315, 321, 882
MboII GAAGA 7 cut(s) 152, 193, 255, 275, 618, 976, 1072
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 1 cut(s) 321
MhlI GDGCHC 2 cut(s) 745, 798
MlsI TGGCCA 1 cut(s) 363
MluCI AATT 2 cut(s) 132, 421
MluNI TGGCCA 1 cut(s) 363
MlyI GAGTC 3 cut(s) 108, 579, 832
MmeI TCCRAC 2 cut(s) 349, 1008
MnlI CCTC 9 cut(s) 152, 168, 179, 181, 425, 756, 866, 1014, 1024
Mox20I TGGCCA 1 cut(s) 363
Mph1103I ATGCAT 1 cut(s) 82
MroNI GCCGGC 1 cut(s) 1046
MscI TGGCCA 1 cut(s) 363
MslI CAYNNNNRTG 1 cut(s) 1055
Msp20I TGGCCA 1 cut(s) 363
MspI CCGG 4 cut(s) 335, 752, 1003, 1047
MspR9I CCNGG 4 cut(s) 335, 387, 548, 844
MunI CAATTG 1 cut(s) 132
Mva1269I GAATGC 2 cut(s) 396, 675
MvaI CCWGG 3 cut(s) 387, 548, 844
MwoI GCNNNNNNNGC 4 cut(s) 215, 681, 988, 997
NaeI GCCGGC 1 cut(s) 1048
NciI CCSGG 1 cut(s) 335
NdeII GATC 3 cut(s) 315, 321, 882
NgoMIV GCCGGC 1 cut(s) 1046
NlaIII CATG 6 cut(s) 353, 511, 583, 667, 790, 834
NlaIV GGNNCC 8 cut(s) 190, 270, 339, 647, 743, 744, 917, 942
NmuCI GTSAC 2 cut(s) 667, 961
NsiI ATGCAT 1 cut(s) 82
OliI CACNNNNGTG 1 cut(s) 1055
PaeR7I CTCGAG 1 cut(s) 593
PciSI GCTCTTC 1 cut(s) 989
PctI GAATGC 2 cut(s) 396, 675
PdiI GCCGGC 1 cut(s) 1048
PfoI TCCNGGA 1 cut(s) 385
PkrI GCNGC 2 cut(s) 1042, 1045
PleI GAGTC 3 cut(s) 108, 579, 831
PmaCI CACGTG 1 cut(s) 722
PmlI CACGTG 1 cut(s) 722
PpsI GAGTC 3 cut(s) 108, 579, 831
Ppu21I YACGTR 1 cut(s) 722
Psp124BI GAGCTC 1 cut(s) 798
Psp6I CCWGG 3 cut(s) 385, 546, 842
PspCI CACGTG 1 cut(s) 722
PspEI GGTNACC 1 cut(s) 667
PspGI CCWGG 3 cut(s) 385, 546, 842
PspN4I GGNNCC 8 cut(s) 190, 270, 339, 647, 743, 744, 917, 942
PspOMI GGGCCC 1 cut(s) 741
PspPI GGNCC 8 cut(s) 254, 268, 403, 436, 646, 741, 742, 941
PspXI VCTCGAGB 1 cut(s) 593
PsuI RGATCY 1 cut(s) 321
RsaI GTAC 2 cut(s) 459, 1037
RsaNI GTAC 2 cut(s) 458, 1036
RseI CAYNNNNRTG 1 cut(s) 1055
SacI GAGCTC 1 cut(s) 798
SapI GCTCTTC 1 cut(s) 989
SatI GCNGC 2 cut(s) 1041, 1044
Sau3AI GATC 3 cut(s) 315, 321, 882
Sau96I GGNCC 8 cut(s) 254, 268, 403, 436, 646, 741, 742, 941
SchI GAGTC 3 cut(s) 108, 579, 832
ScrFI CCNGG 4 cut(s) 335, 387, 548, 844
SduI GDGCHC 2 cut(s) 745, 798
SexAI ACCWGGT 1 cut(s) 842
SfaNI GCATC 1 cut(s) 632
SfcI CTRYAG 1 cut(s) 472
Sfr274I CTCGAG 1 cut(s) 593
SinI GGWCC 2 cut(s) 268, 941
SlaI CTCGAG 1 cut(s) 593
SmiMI CAYNNNNRTG 1 cut(s) 1055
SmlI CTYRAG 1 cut(s) 593
SmoI CTYRAG 1 cut(s) 593
Sse9I AATT 2 cut(s) 132, 421
SsiI CCGC 3 cut(s) 682, 1041, 1044
SspMI CTAG 3 cut(s) 14, 149, 927
SstI GAGCTC 1 cut(s) 798
StyD4I CCNGG 4 cut(s) 333, 385, 546, 842
StyI CCWWGG 4 cut(s) 86, 432, 811, 926
TaaI ACNGT 8 cut(s) 476, 565, 603, 691, 712, 945, 952, 973
TaiI ACGT 2 cut(s) 724, 1092
TaqI TCGA 3 cut(s) 571, 588, 594
TasI AATT 2 cut(s) 132, 421
TauI GCSGC 2 cut(s) 1043, 1046
TscAI CASTG 4 cut(s) 199, 481, 717, 1002
TseFI GTSAC 2 cut(s) 667, 961
Tsp45I GTSAC 2 cut(s) 667, 961
TspDTI ATGAA 7 cut(s) 22, 193, 283, 476, 624, 1069, 1109
TspRI CASTG 4 cut(s) 199, 481, 717, 1002
VpaK11BI GGWCC 2 cut(s) 268, 941
XbaI TCTAGA 1 cut(s) 148
XcmI CCANNNNNNNNNTGG 2 cut(s) 356, 498
XhoI CTCGAG 1 cut(s) 593
XmaJI CCTAGG 1 cut(s) 926
XmiI GTMKAC 2 cut(s) 124, 693
XspI CTAG 3 cut(s) 14, 149, 927
Zsp2I ATGCAT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.