FvH4_3g06640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005905
Physical Location & Seq
Forward (+)
3816322 .. 3819401
3080 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g06640.t1

Sequence Viewer

Length: 465 bp
ATGGAATCAGTTTCCTATTCAAGGAGGGGAGACAGTTTATACATTAGGAGAATCAAACCCAGAAAGAGGGAAGATGAGGATTTAAGTGGGAAAGAGCAAAGAAGAAAGATGGGGATCAAACTCGGCCACAAGAAAAACATAGAGTCGAGTTCTTGCATTGCAGAAGAATCAGACAAAGTGGCCAGCAAGGGATTTTCATTTGAAGACAAAGAGTTACTCGAGCAGCAGATGCATGACATGGCTGTAGTGATTGAGAAATTGGAAAGCAGTAGACAAAAGCTTTTAATGGAGATTGATTCCCAATCTTCGGAGATAGAAAGGCTTTTTGAGGAGAACTCTAATTTATCTAGTTCTCATCAGGAGGCTATGAGCATAGCTGTGCACTGGGAGAATCAGGTGAAAGACTGTCTTAAGCAAAATGAAGAGCTCCGAGGAATCTTAGATAAGAATATAAATGAATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

17.95

Weight (kDa)

5.51

Isoelectric Point (pI)

86.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 271
AclWI GGATC 1 cut(s) 122
AcoI YGGCCR 2 cut(s) 124, 180
AfiI CCNNNNNNNGG 3 cut(s) 21, 66, 307
AflII CTTAAG 1 cut(s) 410
AgsI TTSAA 2 cut(s) 21, 203
AluBI AGCT 3 cut(s) 280, 377, 427
AluI AGCT 3 cut(s) 280, 377, 427
Alw21I GWGCWC 2 cut(s) 384, 429
Alw26I GTCTC 1 cut(s) 24
Alw44I GTGCAC 1 cut(s) 380
AlwI GGATC 1 cut(s) 122
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 2 cut(s) 124, 180
ApaLI GTGCAC 1 cut(s) 380
ApeKI GCWGC 1 cut(s) 223
AsuHPI GGTGA 1 cut(s) 409
AvaI CYCGRG 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 384
BalI TGGCCA 1 cut(s) 182
BanII GRGCYC 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 2 cut(s) 384, 429
BbvI GCAGC 1 cut(s) 235
BccI CCATC 1 cut(s) 103
BcoDI GTCTC 1 cut(s) 24
BfaI CTAG 2 cut(s) 348, 463
BfmI CTRYAG 1 cut(s) 243
BfrI CTTAAG 1 cut(s) 410
BisI GCNGC 1 cut(s) 224
BlsI GCNGC 1 cut(s) 225
BmeT110I CYCGRG 1 cut(s) 218
BmrI ACTGGG 1 cut(s) 394
BmsI GCATC 1 cut(s) 219
BmuI ACTGGG 1 cut(s) 394
BpiI GAAGAC 1 cut(s) 210
BplI GAGNNNNNCTC 2 cut(s) 320, 352
BsaBI GATNNNNATC 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 430
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 66, 307
Bse1I ACTGG 1 cut(s) 389
Bse3DI GCAATG 1 cut(s) 156
Bse8I GATNNNNATC 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 430
BseJI GATNNNNATC 1 cut(s) 113
BseLI CCNNNNNNNGG 3 cut(s) 21, 66, 307
BseMI GCAATG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 389
BseRI GAGGAG 1 cut(s) 344
BseSI GKGCMC 1 cut(s) 384
BseXI GCAGC 1 cut(s) 235
BshFI GGCC 2 cut(s) 126, 182
BsiHKAI GWGCWC 2 cut(s) 384, 429
BsiHKCI CYCGRG 1 cut(s) 218
BslI CCNNNNNNNGG 3 cut(s) 21, 66, 307
BsmAI GTCTC 1 cut(s) 24
BsnI GGCC 2 cut(s) 126, 182
BsoBI CYCGRG 1 cut(s) 218
Bsp1286I GDGCHC 2 cut(s) 384, 429
Bsp143I GATC 1 cut(s) 114
BspANI GGCC 2 cut(s) 126, 182
BspPI GGATC 1 cut(s) 122
BspQI GCTCTTC 1 cut(s) 417
BspTI CTTAAG 1 cut(s) 410
BsrDI GCAATG 1 cut(s) 156
BsrI ACTGG 1 cut(s) 389
BssECI CCNNGG 1 cut(s) 430
BssMI GATC 1 cut(s) 114
Bst4CI ACNGT 2 cut(s) 35, 407
Bst6I CTCTTC 1 cut(s) 417
BstAFI CTTAAG 1 cut(s) 410
BstAPI GCANNNNNTGC 1 cut(s) 229
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 1 cut(s) 439
BstENI CCTNNNNNAGG 1 cut(s) 19
BstKTI GATC 1 cut(s) 117
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 1 cut(s) 114
BstMWI GCNNNNNNNGC 1 cut(s) 229
BstSFI CTRYAG 1 cut(s) 243
BstSLI GKGCMC 1 cut(s) 384
BstV1I GCAGC 1 cut(s) 235
BstV2I GAAGAC 1 cut(s) 210
BsuRI GGCC 2 cut(s) 126, 182
BtsIMutI CAGTG 1 cut(s) 382
Cac8I GCNNGC 1 cut(s) 184
CviAII CATG 2 cut(s) 233, 238
CviJI RGCY 8 cut(s) 126, 182, 242, 280, 322, 365, 377, 427
CviKI_1 RGCY 8 cut(s) 126, 182, 242, 280, 322, 365, 377, 427
DdeI CTNAG 1 cut(s) 439
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
EaeI YGGCCR 2 cut(s) 124, 180
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
Ecl136II GAGCTC 1 cut(s) 427
Eco24I GRGCYC 1 cut(s) 429
Eco53kI GAGCTC 1 cut(s) 427
Eco88I CYCGRG 1 cut(s) 218
EcoICRI GAGCTC 1 cut(s) 427
EcoNI CCTNNNNNAGG 1 cut(s) 19
EcoT22I ATGCAT 1 cut(s) 234
EcoT38I GRGCYC 1 cut(s) 429
FaeI CATG 2 cut(s) 236, 241
FaiI YATR 7 cut(s) 40, 140, 234, 239, 368, 374, 452
FalI AAGNNNNNCTT 2 cut(s) 393, 425
FatI CATG 2 cut(s) 232, 237
FblI GTMKAC 1 cut(s) 271
Fnu4HI GCNGC 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 1 cut(s) 224
FspBI CTAG 2 cut(s) 348, 463
GluI GCNGC 1 cut(s) 224
HaeIII GGCC 2 cut(s) 126, 182
Hin1II CATG 2 cut(s) 236, 241
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 7 cut(s) 5, 51, 143, 167, 296, 391, 435
HphI GGTGA 1 cut(s) 409
Hpy166II GTNNAC 2 cut(s) 272, 382
Hpy188I TCNGA 3 cut(s) 172, 310, 431
Hpy188III TCNNGA 1 cut(s) 359
Hpy8I GTNNAC 2 cut(s) 272, 382
HpyCH4III ACNGT 2 cut(s) 35, 407
HpyCH4V TGCA 4 cut(s) 156, 161, 232, 382
HpyF10VI GCNNNNNNNGC 1 cut(s) 229
HpyF3I CTNAG 1 cut(s) 439
Hsp92II CATG 2 cut(s) 236, 241
Kzo9I GATC 1 cut(s) 114
LguI GCTCTTC 1 cut(s) 417
LmnI GCTCC 1 cut(s) 432
LpnPI CCDG 5 cut(s) 73, 196, 344, 370, 380
Lsp1109I GCAGC 1 cut(s) 235
LweI GCATC 1 cut(s) 219
MaeI CTAG 2 cut(s) 348, 463
MaeIII GTNAC 1 cut(s) 213
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 6 cut(s) 83, 114, 176, 215, 297, 434
MhlI GDGCHC 2 cut(s) 384, 429
MlsI TGGCCA 1 cut(s) 182
MluCI AATT 2 cut(s) 257, 340
MluNI TGGCCA 1 cut(s) 182
MlyI GAGTC 1 cut(s) 152
MnlI CCTC 6 cut(s) 18, 60, 70, 322, 355, 425
Mox20I TGGCCA 1 cut(s) 182
Mph1103I ATGCAT 1 cut(s) 234
MscI TGGCCA 1 cut(s) 182
MseI TTAA 3 cut(s) 83, 284, 411
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 182
MspCI CTTAAG 1 cut(s) 410
MwoI GCNNNNNNNGC 1 cut(s) 229
NdeII GATC 1 cut(s) 114
NlaIII CATG 2 cut(s) 236, 241
NmeAIII GCCGAG 1 cut(s) 102
NsiI ATGCAT 1 cut(s) 234
PaeR7I CTCGAG 1 cut(s) 218
PciSI GCTCTTC 1 cut(s) 417
PfeI GAWTC 6 cut(s) 5, 51, 167, 296, 391, 435
PkrI GCNGC 1 cut(s) 225
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
Psp124BI GAGCTC 1 cut(s) 429
PspXI VCTCGAGB 1 cut(s) 218
RseI CAYNNNNRTG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 429
SapI GCTCTTC 1 cut(s) 417
SaqAI TTAA 3 cut(s) 83, 284, 411
SatI GCNGC 1 cut(s) 224
Sau3AI GATC 1 cut(s) 114
SchI GAGTC 1 cut(s) 152
SduI GDGCHC 2 cut(s) 384, 429
SetI ASST 4 cut(s) 282, 379, 399, 429
SfaNI GCATC 1 cut(s) 219
SfcI CTRYAG 1 cut(s) 243
Sfr274I CTCGAG 1 cut(s) 218
SlaI CTCGAG 1 cut(s) 218
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 2 cut(s) 218, 410
SmoI CTYRAG 2 cut(s) 218, 410
Sse9I AATT 2 cut(s) 257, 340
SspMI CTAG 2 cut(s) 348, 463
SstI GAGCTC 1 cut(s) 429
TaaI ACNGT 2 cut(s) 35, 407
TaqI TCGA 2 cut(s) 146, 219
TasI AATT 2 cut(s) 257, 340
TfiI GAWTC 6 cut(s) 5, 51, 167, 296, 391, 435
Tru1I TTAA 3 cut(s) 83, 284, 411
Tru9I TTAA 3 cut(s) 83, 284, 411
TscAI CASTG 1 cut(s) 389
TseI GCWGC 1 cut(s) 223
TspDTI ATGAA 2 cut(s) 186, 435
TspRI CASTG 1 cut(s) 389
Vha464I CTTAAG 1 cut(s) 410
VneI GTGCAC 1 cut(s) 380
XagI CCTNNNNNAGG 1 cut(s) 19
XhoI CTCGAG 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 271
XspI CTAG 2 cut(s) 348, 463
Zsp2I ATGCAT 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.