Rh3AG021000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0005905
Physical Location & Seq
Forward (+)
1379570 .. 1383126
3557 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG021000.1

Sequence Viewer

Length: 489 bp
ATGGCTATGGGGGAGTGTGAAGAGAAGAAAGTTGTGCATCAAGTGTCAGACAAAGTGGCCAGCAAGGGATTTTCAGTAGAAGACAAGGAGATACTCGAGAAGCAGATGCATGGCATGGCTGTAGTGATTGAGAAATTGGAAAGCAGTAGACAAAAGCTTTCAATGGAGATTGATTCCCAATCTTCGGAGATAGAAAGGCTATTTGAGGAAAACTCTAATTTATCTAGTTCTCTTCAGGAGGCTATGAGTATACCTCTGCACTGGGAGAATCAGGTGAAAGACTATCTTAAGCAAAATGAAGCGCTCCGAGGAATCTTAGATAAGTTAAGAACTGACCAGGCCAAGGGGTTGCTCGACTCCCATCATAATGGCTCTAACAAGACTGGTTTAGCTGAATATACACCTGAAGTTCTCCCTATCAAGGATCAACTTGCAAAAGAACAGAGCAAAGCAGAGGCATTATCAGCTGTTGTATATGGAGGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

18.07

Weight (kDa)

5.18

Isoelectric Point (pI)

57.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 148, 250
AclWI GGATC 1 cut(s) 432
AcoI YGGCCR 1 cut(s) 57
AcuI CTGAAG 2 cut(s) 218, 426
AfeI AGCGCT 1 cut(s) 303
AfiI CCNNNNNNNGG 3 cut(s) 184, 343, 421
AflII CTTAAG 1 cut(s) 287
AgsI TTSAA 1 cut(s) 162
AjnI CCWGG 1 cut(s) 336
AluBI AGCT 3 cut(s) 157, 392, 467
AluI AGCT 3 cut(s) 157, 392, 467
AlwI GGATC 1 cut(s) 432
Ama87I CYCGRG 1 cut(s) 95
Aor51HI AGCGCT 1 cut(s) 303
AoxI GGCC 2 cut(s) 57, 339
AspLEI GCGC 1 cut(s) 304
AsuHPI GGTGA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 95
BalI TGGCCA 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 87
BccI CCATC 1 cut(s) 369
BciT130I CCWGG 1 cut(s) 338
BfaI CTAG 1 cut(s) 225
BfmI CTRYAG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 305
BfrI CTTAAG 1 cut(s) 287
Bme1390I CCNGG 1 cut(s) 338
BmeT110I CYCGRG 1 cut(s) 95
BmrFI CCNGG 1 cut(s) 338
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 2 cut(s) 46, 96
BmuI ACTGGG 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 87
BplI GAGNNNNNCTC 4 cut(s) 197, 229, 238, 270
BsaJI CCNNGG 2 cut(s) 307, 342
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 343, 421
Bse1I ACTGG 2 cut(s) 266, 388
BseBI CCWGG 1 cut(s) 338
BseDI CCNNGG 2 cut(s) 307, 342
BseLI CCNNNNNNNGG 3 cut(s) 184, 343, 421
BseNI ACTGG 2 cut(s) 266, 388
BsgI GTGCAG 1 cut(s) 242
BshFI GGCC 2 cut(s) 59, 341
BsiHKCI CYCGRG 1 cut(s) 95
BslI CCNNNNNNNGG 3 cut(s) 184, 343, 421
BsnI GGCC 2 cut(s) 59, 341
BsoBI CYCGRG 1 cut(s) 95
Bsp143I GATC 1 cut(s) 424
BspANI GGCC 2 cut(s) 59, 341
BspPI GGATC 1 cut(s) 432
BspTI CTTAAG 1 cut(s) 287
BsrI ACTGG 2 cut(s) 266, 388
BssECI CCNNGG 2 cut(s) 307, 342
BssMI GATC 1 cut(s) 424
BssNAI GTATAC 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 342
Bst1107I GTATAC 1 cut(s) 251
Bst2UI CCWGG 1 cut(s) 338
Bst6I CTCTTC 2 cut(s) 15, 237
BstAFI CTTAAG 1 cut(s) 287
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 1 cut(s) 316
BstH2I RGCGCY 1 cut(s) 305
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 1 cut(s) 427
BstMBI GATC 1 cut(s) 424
BstMWI GCNNNNNNNGC 1 cut(s) 464
BstNI CCWGG 1 cut(s) 338
BstSCI CCNGG 1 cut(s) 336
BstSFI CTRYAG 1 cut(s) 120
BstV2I GAAGAC 1 cut(s) 87
BstXI CCANNNNNNTGG 1 cut(s) 368
BstZ17I GTATAC 1 cut(s) 251
BsuRI GGCC 2 cut(s) 59, 341
BtsIMutI CAGTG 1 cut(s) 259
Cac8I GCNNGC 1 cut(s) 61
CfoI GCGC 1 cut(s) 304
CviAII CATG 2 cut(s) 110, 115
DdeI CTNAG 1 cut(s) 316
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 2 cut(s) 15, 237
EarI CTCTTC 2 cut(s) 15, 237
Eco130I CCWWGG 1 cut(s) 342
Eco47III AGCGCT 1 cut(s) 303
Eco57I CTGAAG 2 cut(s) 218, 426
Eco88I CYCGRG 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 336
EcoT14I CCWWGG 1 cut(s) 342
EcoT22I ATGCAT 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 2 cut(s) 113, 118
FaiI YATR 9 cut(s) 8, 111, 116, 245, 251, 366, 399, 475, 477
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FatI CATG 2 cut(s) 109, 114
FblI GTMKAC 2 cut(s) 148, 250
FspBI CTAG 1 cut(s) 225
GlaI GCGC 1 cut(s) 303
HaeII RGCGCY 1 cut(s) 305
HaeIII GGCC 2 cut(s) 59, 341
HhaI GCGC 1 cut(s) 304
Hin1II CATG 2 cut(s) 113, 118
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 4 cut(s) 173, 268, 312, 356
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 2 cut(s) 149, 251
Hpy188I TCNGA 3 cut(s) 49, 187, 308
Hpy188III TCNNGA 2 cut(s) 97, 236
Hpy8I GTNNAC 2 cut(s) 149, 251
HpyCH4V TGCA 4 cut(s) 37, 109, 259, 434
HpyF10VI GCNNNNNNNGC 1 cut(s) 464
HpyF3I CTNAG 1 cut(s) 316
Hsp92II CATG 2 cut(s) 113, 118
HspAI GCGC 1 cut(s) 302
Kzo9I GATC 1 cut(s) 424
LmnI GCTCC 1 cut(s) 309
LpnPI CCDG 8 cut(s) 73, 221, 247, 257, 323, 350, 369, 417
LweI GCATC 2 cut(s) 46, 96
MaeI CTAG 1 cut(s) 225
MalI GATC 1 cut(s) 426
MboI GATC 1 cut(s) 424
MboII GAAGA 5 cut(s) 32, 37, 92, 174, 224
MlsI TGGCCA 1 cut(s) 59
MluCI AATT 2 cut(s) 134, 217
MluNI TGGCCA 1 cut(s) 59
MlyI GAGTC 1 cut(s) 350
MnlI CCTC 6 cut(s) 199, 232, 264, 302, 448, 473
Mox20I TGGCCA 1 cut(s) 59
Mph1103I ATGCAT 1 cut(s) 111
MscI TGGCCA 1 cut(s) 59
MseI TTAA 2 cut(s) 288, 326
MslI CAYNNNNRTG 1 cut(s) 366
Msp20I TGGCCA 1 cut(s) 59
MspA1I CMGCKG 1 cut(s) 467
MspCI CTTAAG 1 cut(s) 287
MspR9I CCNGG 1 cut(s) 338
MvaI CCWGG 1 cut(s) 338
MwoI GCNNNNNNNGC 1 cut(s) 464
NdeII GATC 1 cut(s) 424
NlaIII CATG 2 cut(s) 113, 118
NsiI ATGCAT 1 cut(s) 111
PaeR7I CTCGAG 1 cut(s) 95
PfeI GAWTC 3 cut(s) 173, 268, 312
PleI GAGTC 1 cut(s) 350
PpsI GAGTC 1 cut(s) 350
Psp6I CCWGG 1 cut(s) 336
PspGI CCWGG 1 cut(s) 336
PvuII CAGCTG 1 cut(s) 467
RseI CAYNNNNRTG 1 cut(s) 366
SaqAI TTAA 2 cut(s) 288, 326
Sau3AI GATC 1 cut(s) 424
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 1 cut(s) 338
SetI ASST 6 cut(s) 159, 256, 276, 394, 406, 469
SfaNI GCATC 2 cut(s) 46, 96
SfcI CTRYAG 1 cut(s) 120
Sfr274I CTCGAG 1 cut(s) 95
SlaI CTCGAG 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 366
SmlI CTYRAG 2 cut(s) 95, 287
SmoI CTYRAG 2 cut(s) 95, 287
Sse9I AATT 2 cut(s) 134, 217
SspMI CTAG 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 336
StyI CCWWGG 1 cut(s) 342
TaqI TCGA 2 cut(s) 96, 354
TasI AATT 2 cut(s) 134, 217
TfiI GAWTC 3 cut(s) 173, 268, 312
Tru1I TTAA 2 cut(s) 288, 326
Tru9I TTAA 2 cut(s) 288, 326
TscAI CASTG 1 cut(s) 266
TspDTI ATGAA 1 cut(s) 312
TspRI CASTG 1 cut(s) 266
Vha464I CTTAAG 1 cut(s) 287
XhoI CTCGAG 1 cut(s) 95
XmiI GTMKAC 2 cut(s) 148, 250
XspI CTAG 1 cut(s) 225
Zsp2I ATGCAT 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.