FvH4_3g10000

Serine threonine-protein phosphatase 6 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002321
Physical Location & Seq
Forward (+)
5818216 .. 5826820
8605 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g10000.t1

Sequence Viewer

Length: 2322 bp
ATGTTTTGGCGTATGGCCGGCCTGTCCACGGCGTCTCCGGTGGAGACGATTTTGGACAAGGATAATTTCACATTGGAAGAGCTTCTTGATGAGGATGAAATTATTCAAGAATGCAAAGCTCTTAATGGGCGGCTAATCAATTTCTTGCGAGAAAGAGTGCAGGTGGAACAACTCATCCGATATATTGTTGAAGAAGCTCCCGAGGATGCTGAAAAGAGGCGAACTTTTAAATTCCCTTTTATCGCATGTGAGATCTTTACTTGTGAAGTTGATATCATACTCAAAACATTGGTAGAGGATGAGGAGTTGATGAATTTGTTGTTCTCCTTTTTGGAACCAAATCATTCCCATAGCACACTCTTGGCTGGCTACTTCAGCAAGGTTGTTGTTTGCCTGCTGTTGCGTAAGGCAGTTCCGTTTCTGCATTATATTCAGAGTCATCAAGATATTGTCAAGAAGCTAGTTGACCTGATTGGAACAACATCAATCATGGAGGTTTTGATACGTCTGGTTGGTGCTGATGAACATATGTATTCAAACTACACGGAAGCAATGCACTGGATACAAGAGACTGATGTGCTAGAGATGATTGTGGACAAGTTCAGCTCCTCAGATTGTCCTGAAGTGCATGCCAATGCAGCAGAGACGCTTTGTGCTATAACTCGGTTTGCTCCACCTGGACTTTCTACTAAAATCTCTAGCCCAAGTTTCATTGGAAGATTGTTTCGGCATGCTTTGGAGGGCTCCCGGCCAAAATCTGTTCTCGTTAACTCTTTGTCAGTATGCATATCTTTGTTAGACCCTAGGAGGCTTAGTTTGGGAACATATCAGATGTATGGACGTCAAATGACTCATGGATCCACAGTTACTGCCAACCCTGAAACTGTTGTCGGCATGTTGGAGAGCCTAGGTGATTTGCTGAAGCTTTTAGATGTGTCATCAGTAGACAATACGTTGTTAACTACATATGGCAAGTTACAGCCGCCTCTTGGAAAACATCGCTTAAAGGTGGTAGAATTCATCTCGGTATTACTTACAGTTGGTAGTGAAGCTGCTGAGAAGGAATTGATTAGTCTTGGAGCAGTTCAAAAAATTTTAGACTTGTTTTTCAAGTACCCGTATAACAACTTTGTACATCACCACATAGAGAATATAATAGTGTCATGTTTGGAAAGCAAGAATGCTCCTCTTGTTGAGCATCTTCTTCGCGAATGCGATCTTGTTGGAAAAATACTTCAAGCAGAAAAGAATTTTACATTGACAGCTGACTCAGACAAGCCAACTATACCTGCTGAGGGCAGATCCGCACCCAGGATAGGAAATATTGGACATTTAACACGTATATCCAATAAAATCATTCAGCTGGGGAATAATAACAGCGAGATTGAGGCATATCTGCAGGAGAACAGCGAGTGGACTGATTGGCAGACAAATGTTCTATCAAAGCGTAATGCATTGGAAAATGTCTATCAGTGGGCATGTGGGCGACCAACAGCCTTGCATGATCGAAATAGGGATAGTGATGATGATGATTACCAAGATAGAGACTACGACGTTGCAGCTCTTGCAAATAACTTGAGCCAGGCTTTTCGGTATGGCCTTTACAACAATGATGATTTAGATGAGGGCCAGGGCTCACTTGAACGAGATGATGAGGATGTCTACTTTGATGATGAATCTGCTGAAGTTGTTATCTCATCACTGCGACTTGGAGATGACCAGGAAGGCGGTTCTCTGTTTACGAATTCCAACTGGTTTGCATTTGAGGATGACAGGAGTGATCGTGCAACTGGGTCCCTTACCTCTCCATCTAATACCCTGGAGGCTGATGTTACCGATGGTGGTGGAGAGGATAATGTCATTGTTGGTGAAGATGATGACTTGGTTGATACTGCAACATCTCCAAATTCAGGAACAAAACCAGAAGATTCAGAAGTTAGTGTGCCTAACAGTTCTACAGAGGTGGGATCCAGTGAAACTGATAAGCCACCAGAGTGGGTTGAATGGAGAGAAACTTCAGATTCTGGGGAACCATCCGATGCCCTACCAAATGGTGAGCTTCAAACGATATCTGCTGATGCAGTGACAAGTTTTCCATCCTCTGATCCTTTAGTAAGTAATGAGGAAATTGTTGCTGGACATGAAGCTGAAATATCAGCTGAGAAAACCTCAAAACCTTGTGAGGTTTCAGATTCTGGTAATGAGAATCCAAGTTCTGATTCAACCACTTCTGACGCTGCAGCAAGTGCTGTTGAAACAAAGGACACTGAAGAAGCTGCAGACACAGTAGGGGATAAAGAAGCTGACAAGGAAGCGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAPS

Protein Analysis

774

Amino Acids

85.5

Weight (kDa)

4.42

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAPS PF04499 131 - 489 3.5e-66 SIT4 phosphatase-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 151
AatII GACGTC 1 cut(s) 844
Acc36I ACCTGC 2 cut(s) 151, 1297
AccI GTMKAC 2 cut(s) 945, 1662
AccII CGCG 1 cut(s) 1209
AciI CCGC 4 cut(s) 130, 983, 1305, 1728
AclWI GGATC 6 cut(s) 852, 865, 1296, 1962, 1975, 2099
AcoI YGGCCR 2 cut(s) 15, 749
AcsI RAATTY 7 cut(s) 230, 313, 1016, 1092, 1249, 1744, 1906
AcuI CTGAAG 6 cut(s) 358, 642, 941, 1704, 2001, 2289
AcyI GRCGYC 2 cut(s) 32, 841
AfaI GTAC 2 cut(s) 1115, 1134
AfiI CCNNNNNNNGG 6 cut(s) 28, 989, 1295, 1311, 1316, 1910
AflIII ACRYGT 1 cut(s) 1337
AjnI CCWGG 6 cut(s) 676, 1310, 1581, 1629, 1719, 1818
AjuI GAANNNNNNNTTGG 2 cut(s) 2203, 2235
AleI CACNNNNGTG 1 cut(s) 1993
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
Alw26I GTCTC 5 cut(s) 38, 39, 563, 638, 1539
AlwI GGATC 6 cut(s) 852, 865, 1296, 1962, 1975, 2099
AlwNI CAGNNNCTG 3 cut(s) 869, 2024, 2195
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 5 cut(s) 15, 19, 749, 1597, 1627
ApeKI GCWGC 6 cut(s) 638, 1052, 1559, 2237, 2240, 2276
ApoI RAATTY 7 cut(s) 230, 313, 1016, 1092, 1249, 1744, 1906
Asp700I GAANNNNTTC 2 cut(s) 81, 102
AspA2I CCTAGG 2 cut(s) 803, 907
AspS9I GGNCC 2 cut(s) 1627, 1794
AsuC2I CCSGG 1 cut(s) 748
AsuHPI GGTGA 4 cut(s) 923, 1130, 1880, 2066
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 1 cut(s) 1794
AvrII CCTAGG 2 cut(s) 803, 907
BamHI GGATCC 2 cut(s) 857, 1967
BanII GRGCYC 2 cut(s) 746, 1637
BbvCI CCTCAGC 1 cut(s) 1293
BbvI GCAGC 6 cut(s) 650, 1039, 1571, 2224, 2252, 2263
BccI CCATC 4 cut(s) 1816, 1832, 2041, 2104
BceAI ACGGC 1 cut(s) 45
BcgI CGANNNNNNTGC 2 cut(s) 1187, 1221
BciT130I CCWGG 6 cut(s) 678, 1312, 1583, 1631, 1721, 1820
BciVI GTATCC 1 cut(s) 555
BcnI CCSGG 1 cut(s) 748
BcoDI GTCTC 5 cut(s) 38, 39, 563, 638, 1539
BfaI CTAG 5 cut(s) 461, 581, 699, 804, 908
BfmI CTRYAG 4 cut(s) 1397, 1956, 2238, 2277
BfuAI ACCTGC 2 cut(s) 151, 1297
BfuI GTATCC 1 cut(s) 555
BglII AGATCT 1 cut(s) 252
BisI GCNGC 8 cut(s) 131, 639, 983, 1053, 1560, 2238, 2241, 2277
BlnI CCTAGG 2 cut(s) 803, 907
BlsI GCNGC 8 cut(s) 132, 640, 984, 1054, 1561, 2239, 2242, 2278
Bme1390I CCNGG 7 cut(s) 678, 748, 1312, 1583, 1631, 1721, 1820
Bme18I GGWCC 1 cut(s) 1794
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 2 cut(s) 1627, 1794
BmiI GGNNCC 7 cut(s) 336, 745, 859, 1795, 1796, 1969, 2031
BmrFI CCNGG 7 cut(s) 678, 748, 1312, 1583, 1631, 1721, 1820
BmrI ACTGGG 1 cut(s) 1800
BmsI GCATC 4 cut(s) 196, 1207, 2029, 2068
BmuI ACTGGG 1 cut(s) 1800
BplI GAGNNNNNCTC 2 cut(s) 2153, 2185
BpmI CTGGAG 1 cut(s) 1841
Bpu10I CCTNAGC 1 cut(s) 1293
BpuEI CTTGAG 1 cut(s) 1597
BpuMI CCSGG 1 cut(s) 748
BsaAI YACGTR 1 cut(s) 1340
BsaHI GRCGYC 2 cut(s) 32, 841
BsaJI CCNNGG 7 cut(s) 27, 201, 803, 907, 1310, 1630, 1818
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 6 cut(s) 19, 49, 799, 829, 1768, 1798
Bsc4I CCNNNNNNNGG 6 cut(s) 28, 989, 1295, 1311, 1316, 1910
Bse118I RCCGGY 1 cut(s) 17
Bse1I ACTGG 4 cut(s) 563, 1757, 1795, 1971
Bse3DI GCAATG 1 cut(s) 558
BseBI CCWGG 6 cut(s) 678, 1312, 1583, 1631, 1721, 1820
BseDI CCNNGG 7 cut(s) 27, 201, 803, 907, 1310, 1630, 1818
BseGI GGATG 8 cut(s) 100, 174, 211, 304, 1663, 1774, 2033, 2096
BseLI CCNNNNNNNGG 6 cut(s) 28, 989, 1295, 1311, 1316, 1910
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 5 cut(s) 624, 1047, 1284, 1284, 2151
BseNI ACTGG 4 cut(s) 563, 1757, 1795, 1971
BseRI GAGGAG 3 cut(s) 317, 598, 1176
BseXI GCAGC 6 cut(s) 650, 1039, 1571, 2224, 2252, 2263
BseYI CCCAGC 1 cut(s) 1363
BsgI GTGCAG 1 cut(s) 179
Bsh1236I CGCG 1 cut(s) 1209
BshFI GGCC 5 cut(s) 17, 21, 751, 1599, 1629
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 3 cut(s) 18, 38, 748
BslFI GGGAC 1 cut(s) 1780
BslI CCNNNNNNNGG 6 cut(s) 28, 989, 1295, 1311, 1316, 1910
BsmAI GTCTC 5 cut(s) 38, 39, 563, 638, 1539
BsmBI CGTCTC 3 cut(s) 38, 39, 638
BsmFI GGGAC 1 cut(s) 1780
BsmI GAATGC 3 cut(s) 116, 1186, 1217
BsnI GGCC 5 cut(s) 17, 21, 751, 1599, 1629
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 746, 1637
Bsp1407I TGTACA 1 cut(s) 1132
Bsp143I GATC 8 cut(s) 252, 857, 1216, 1301, 1504, 1780, 1967, 2104
Bsp68I TCGCGA 1 cut(s) 1209
BspACI CCGC 4 cut(s) 130, 983, 1305, 1728
BspANI GGCC 5 cut(s) 17, 21, 751, 1599, 1629
BspCNI CTCAG 5 cut(s) 623, 1048, 1283, 1285, 2152
BspFNI CGCG 1 cut(s) 1209
BspLI GGNNCC 7 cut(s) 336, 745, 859, 1795, 1796, 1969, 2031
BspMAI CTGCAG 3 cut(s) 1401, 2242, 2281
BspMI ACCTGC 2 cut(s) 151, 1297
BspPI GGATC 6 cut(s) 852, 865, 1296, 1962, 1975, 2099
BspQI GCTCTTC 1 cut(s) 72
BsrDI GCAATG 1 cut(s) 558
BsrFI RCCGGY 1 cut(s) 17
BsrGI TGTACA 1 cut(s) 1132
BsrI ACTGG 4 cut(s) 563, 1757, 1795, 1971
BssAI RCCGGY 1 cut(s) 17
BssECI CCNNGG 7 cut(s) 27, 201, 803, 907, 1310, 1630, 1818
BssMI GATC 8 cut(s) 252, 857, 1216, 1301, 1504, 1780, 1967, 2104
BssNI GRCGYC 2 cut(s) 32, 841
BssT1I CCWWGG 2 cut(s) 803, 907
Bst2UI CCWGG 6 cut(s) 678, 1312, 1583, 1631, 1721, 1820
Bst4CI ACNGT 5 cut(s) 865, 886, 1039, 1952, 2287
Bst6I CTCTTC 1 cut(s) 72
BstACI GRCGYC 2 cut(s) 32, 841
BstAPI GCANNNNNTGC 2 cut(s) 1565, 2246
BstAUI TGTACA 1 cut(s) 1132
BstBAI YACGTR 1 cut(s) 1340
BstC8I GCNNGC 5 cut(s) 19, 367, 395, 630, 732
BstDEI CTNAG 6 cut(s) 610, 812, 1056, 1270, 1293, 2160
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 8 cut(s) 100, 174, 211, 304, 1663, 1774, 2033, 2096
BstFNI CGCG 1 cut(s) 1209
BstKTI GATC 8 cut(s) 255, 860, 1219, 1304, 1507, 1783, 1970, 2107
BstMAI GTCTC 5 cut(s) 38, 39, 563, 638, 1539
BstMBI GATC 8 cut(s) 252, 857, 1216, 1301, 1504, 1780, 1967, 2104
BstMWI GCNNNNNNNGC 4 cut(s) 375, 638, 1565, 2246
BstNI CCWGG 6 cut(s) 678, 1312, 1583, 1631, 1721, 1820
BstNSI RCATGY 5 cut(s) 249, 632, 734, 898, 1482
BstSCI CCNGG 7 cut(s) 676, 746, 1310, 1581, 1629, 1719, 1818
BstSFI CTRYAG 4 cut(s) 1397, 1956, 2238, 2277
BstUI CGCG 1 cut(s) 1209
BstV1I GCAGC 6 cut(s) 650, 1039, 1571, 2224, 2252, 2263
BstX2I RGATCY 4 cut(s) 252, 857, 1301, 1967
BstXI CCANNNNNNTGG 1 cut(s) 1995
BstYI RGATCY 4 cut(s) 252, 857, 1301, 1967
BsuI GTATCC 1 cut(s) 555
BsuRI GGCC 5 cut(s) 17, 21, 751, 1599, 1629
BtgI CCRYGG 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 983
BtsCI GGATG 8 cut(s) 100, 174, 211, 304, 1663, 1774, 2033, 2096
BtsI GCAGTG 2 cut(s) 1700, 2088
BtsIMutI CAGTG 6 cut(s) 556, 1478, 1700, 1978, 2088, 2265
BtuMI TCGCGA 1 cut(s) 1209
BveI ACCTGC 2 cut(s) 151, 1297
Cac8I GCNNGC 5 cut(s) 19, 367, 395, 630, 732
CaiI CAGNNNCTG 3 cut(s) 869, 2024, 2195
Cfr10I RCCGGY 1 cut(s) 17
Cfr13I GGNCC 2 cut(s) 1627, 1794
CseI GACGC 3 cut(s) 21, 655, 2243
Csp6I GTAC 2 cut(s) 1114, 1133
CviQI GTAC 2 cut(s) 1114, 1133
DdeI CTNAG 6 cut(s) 610, 812, 1056, 1270, 1293, 2160
DpnI GATC 8 cut(s) 254, 859, 1218, 1303, 1506, 1782, 1969, 2106
DpnII GATC 8 cut(s) 252, 857, 1216, 1301, 1504, 1780, 1967, 2104
DraI TTTAAA 1 cut(s) 229
EaeI YGGCCR 2 cut(s) 15, 749
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
Eco130I CCWWGG 2 cut(s) 803, 907
Eco24I GRGCYC 2 cut(s) 746, 1637
Eco32I GATATC 2 cut(s) 274, 2070
Eco47I GGWCC 1 cut(s) 1794
Eco57I CTGAAG 6 cut(s) 358, 642, 941, 1704, 2001, 2289
Eco88I CYCGRG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 1794
EcoRI GAATTC 2 cut(s) 1016, 1744
EcoRII CCWGG 6 cut(s) 676, 1310, 1581, 1629, 1719, 1818
EcoRV GATATC 2 cut(s) 274, 2070
EcoT14I CCWWGG 2 cut(s) 803, 907
EcoT22I ATGCAT 2 cut(s) 788, 1456
EcoT38I GRGCYC 2 cut(s) 746, 1637
ErhI CCWWGG 2 cut(s) 803, 907
Esp3I CGTCTC 3 cut(s) 38, 39, 638
FalI AAGNNNNNCTT 2 cut(s) 69, 101
FaqI GGGAC 1 cut(s) 1780
FauNDI CATATG 2 cut(s) 528, 967
FblI GTMKAC 2 cut(s) 945, 1662
Fnu4HI GCNGC 8 cut(s) 131, 639, 983, 1053, 1560, 2238, 2241, 2277
FokI GGATG 8 cut(s) 107, 161, 218, 311, 1670, 1781, 2020, 2083
FriOI GRGCYC 2 cut(s) 746, 1637
FseI GGCCGGCC 1 cut(s) 21
Fsp4HI GCNGC 8 cut(s) 131, 639, 983, 1053, 1560, 2238, 2241, 2277
FspBI CTAG 5 cut(s) 461, 581, 699, 804, 908
GluI GCNGC 8 cut(s) 131, 639, 983, 1053, 1560, 2238, 2241, 2277
GsaI CCCAGC 1 cut(s) 1367
GsuI CTGGAG 1 cut(s) 1841
HaeIII GGCC 5 cut(s) 17, 21, 751, 1599, 1629
HapII CCGG 3 cut(s) 18, 38, 748
HgaI GACGC 3 cut(s) 21, 655, 2243
Hin1I GRCGYC 2 cut(s) 32, 841
HincII GTYRAC 3 cut(s) 466, 769, 960
HindII GTYRAC 3 cut(s) 466, 769, 960
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 9 cut(s) 436, 850, 1268, 1676, 1928, 2021, 2192, 2206, 2219
HpaI GTTAAC 2 cut(s) 769, 960
HpaII CCGG 3 cut(s) 18, 38, 748
HphI GGTGA 4 cut(s) 923, 1130, 1880, 2066
Hpy166II GTNNAC 9 cut(s) 27, 466, 595, 769, 946, 960, 1416, 1663, 1740
Hpy188III TCNNGA 8 cut(s) 86, 107, 200, 443, 454, 620, 1208, 1911
Hpy8I GTNNAC 9 cut(s) 27, 466, 595, 769, 946, 960, 1416, 1663, 1740
Hpy99I CGWCG 1 cut(s) 1556
HpyAV CCTTC 2 cut(s) 1054, 1718
HpyCH4III ACNGT 5 cut(s) 865, 886, 1039, 1952, 2287
HpyCH4IV ACGT 5 cut(s) 505, 841, 953, 1339, 1554
HpyF10VI GCNNNNNNNGC 4 cut(s) 375, 638, 1565, 2246
HpyF3I CTNAG 6 cut(s) 610, 812, 1056, 1270, 1293, 2160
HpySE526I ACGT 5 cut(s) 505, 841, 953, 1339, 1554
Hsp92I GRCGYC 2 cut(s) 32, 841
KflI GGGWCCC 1 cut(s) 1794
KroI GCCGGC 1 cut(s) 17
KroNI GCCGGC 1 cut(s) 19
KspAI GTTAAC 2 cut(s) 769, 960
Kzo9I GATC 8 cut(s) 252, 857, 1216, 1301, 1504, 1780, 1967, 2104
LguI GCTCTTC 1 cut(s) 72
LmnI GCTCC 6 cut(s) 202, 611, 676, 749, 1079, 1189
Lsp1109I GCAGC 6 cut(s) 650, 1039, 1571, 2224, 2252, 2263
LweI GCATC 4 cut(s) 196, 1207, 2029, 2068
MaeI CTAG 5 cut(s) 461, 581, 699, 804, 908
MaeII ACGT 5 cut(s) 505, 841, 953, 1339, 1554
MaeIII GTNAC 4 cut(s) 865, 975, 1831, 2083
MalI GATC 8 cut(s) 254, 859, 1218, 1303, 1506, 1782, 1969, 2106
MboI GATC 8 cut(s) 252, 857, 1216, 1301, 1504, 1780, 1967, 2104
MboII GAAGA 8 cut(s) 89, 203, 729, 1193, 1196, 1883, 1937, 2282
MflI RGATCY 4 cut(s) 252, 857, 1301, 1967
MhlI GDGCHC 2 cut(s) 746, 1637
MlyI GAGTC 3 cut(s) 445, 844, 1262
MmeI TCCRAC 3 cut(s) 879, 1204, 1773
Mph1103I ATGCAT 2 cut(s) 788, 1456
MroNI GCCGGC 1 cut(s) 17
MroXI GAANNNNTTC 2 cut(s) 81, 102
MseI TTAA 6 cut(s) 123, 228, 768, 959, 1004, 1334
MslI CAYNNNNRTG 2 cut(s) 633, 1993
MspA1I CMGCKG 3 cut(s) 1265, 1363, 2159
MspI CCGG 3 cut(s) 18, 38, 748
MspR9I CCNGG 7 cut(s) 678, 748, 1312, 1583, 1631, 1721, 1820
Mva1269I GAATGC 3 cut(s) 116, 1186, 1217
MvaI CCWGG 6 cut(s) 678, 1312, 1583, 1631, 1721, 1820
MvnI CGCG 1 cut(s) 1209
MwoI GCNNNNNNNGC 4 cut(s) 375, 638, 1565, 2246
NaeI GCCGGC 1 cut(s) 19
NciI CCSGG 1 cut(s) 748
NdeI CATATG 2 cut(s) 528, 967
NdeII GATC 8 cut(s) 252, 857, 1216, 1301, 1504, 1780, 1967, 2104
NgoMIV GCCGGC 1 cut(s) 17
NlaIV GGNNCC 7 cut(s) 336, 745, 859, 1795, 1796, 1969, 2031
NmuCI GTSAC 1 cut(s) 2083
NruI TCGCGA 1 cut(s) 1209
NsiI ATGCAT 2 cut(s) 788, 1456
NspI RCATGY 5 cut(s) 249, 632, 734, 898, 1482
OliI CACNNNNGTG 1 cut(s) 1993
PaeI GCATGC 2 cut(s) 632, 734
PaqCI CACCTGC 1 cut(s) 151
PciSI GCTCTTC 1 cut(s) 72
PctI GAATGC 3 cut(s) 116, 1186, 1217
PdiI GCCGGC 1 cut(s) 19
PdmI GAANNNNTTC 2 cut(s) 81, 102
PfeI GAWTC 6 cut(s) 1676, 1928, 2021, 2192, 2206, 2219
PkrI GCNGC 8 cut(s) 132, 640, 984, 1054, 1561, 2239, 2242, 2278
PleI GAGTC 3 cut(s) 444, 844, 1262
PpsI GAGTC 3 cut(s) 444, 844, 1262
Ppu21I YACGTR 1 cut(s) 1340
PpuMI RGGWCCY 1 cut(s) 1794
Psp5II RGGWCCY 1 cut(s) 1794
Psp6I CCWGG 6 cut(s) 676, 1310, 1581, 1629, 1719, 1818
PspFI CCCAGC 1 cut(s) 1363
PspGI CCWGG 6 cut(s) 676, 1310, 1581, 1629, 1719, 1818
PspN4I GGNNCC 7 cut(s) 336, 745, 859, 1795, 1796, 1969, 2031
PspPI GGNCC 2 cut(s) 1627, 1794
PspPPI RGGWCCY 1 cut(s) 1794
PstI CTGCAG 3 cut(s) 1401, 2242, 2281
PstNI CAGNNNCTG 3 cut(s) 869, 2024, 2195
PsuI RGATCY 4 cut(s) 252, 857, 1301, 1967
PvuII CAGCTG 3 cut(s) 1265, 1363, 2159
RigI GGCCGGCC 1 cut(s) 21
RruI TCGCGA 1 cut(s) 1209
RsaI GTAC 2 cut(s) 1115, 1134
RsaNI GTAC 2 cut(s) 1114, 1133
RseI CAYNNNNRTG 2 cut(s) 633, 1993
SapI GCTCTTC 1 cut(s) 72
SaqAI TTAA 6 cut(s) 123, 228, 768, 959, 1004, 1334
SatI GCNGC 8 cut(s) 131, 639, 983, 1053, 1560, 2238, 2241, 2277
Sau3AI GATC 8 cut(s) 252, 857, 1216, 1301, 1504, 1780, 1967, 2104
Sau96I GGNCC 2 cut(s) 1627, 1794
SchI GAGTC 3 cut(s) 445, 844, 1262
ScrFI CCNGG 7 cut(s) 678, 748, 1312, 1583, 1631, 1721, 1820
SduI GDGCHC 2 cut(s) 746, 1637
SfaNI GCATC 4 cut(s) 196, 1207, 2029, 2068
SfcI CTRYAG 4 cut(s) 1397, 1956, 2238, 2277
SinI GGWCC 1 cut(s) 1794
SmiMI CAYNNNNRTG 2 cut(s) 633, 1993
SmlI CTYRAG 1 cut(s) 1576
SmoI CTYRAG 1 cut(s) 1576
SphI GCATGC 2 cut(s) 632, 734
SsiI CCGC 4 cut(s) 130, 983, 1305, 1728
SspI AATATT 1 cut(s) 1324
SspMI CTAG 5 cut(s) 461, 581, 699, 804, 908
StyD4I CCNGG 7 cut(s) 676, 746, 1310, 1581, 1629, 1719, 1818
StyI CCWWGG 2 cut(s) 803, 907
TaaI ACNGT 5 cut(s) 865, 886, 1039, 1952, 2287
TaiI ACGT 5 cut(s) 508, 844, 956, 1342, 1557
TaqI TCGA 1 cut(s) 1507
TatI WGTACW 1 cut(s) 1132
TauI GCSGC 2 cut(s) 133, 985
TfiI GAWTC 6 cut(s) 1676, 1928, 2021, 2192, 2206, 2219
Tru1I TTAA 6 cut(s) 123, 228, 768, 959, 1004, 1334
Tru9I TTAA 6 cut(s) 123, 228, 768, 959, 1004, 1334
TscAI CASTG 6 cut(s) 563, 1478, 1707, 1978, 2088, 2272
TseFI GTSAC 1 cut(s) 2083
TseI GCWGC 6 cut(s) 638, 1052, 1559, 2237, 2240, 2276
Tsp45I GTSAC 1 cut(s) 2083
TspDTI ATGAA 7 cut(s) 111, 326, 537, 700, 1009, 1689, 2157
TspGWI ACGGA 2 cut(s) 405, 560
TspRI CASTG 6 cut(s) 563, 1478, 1707, 1978, 2088, 2272
VpaK11BI GGWCC 1 cut(s) 1794
XapI RAATTY 7 cut(s) 230, 313, 1016, 1092, 1249, 1744, 1906
XceI RCATGY 5 cut(s) 249, 632, 734, 898, 1482
XmaJI CCTAGG 2 cut(s) 803, 907
XmiI GTMKAC 2 cut(s) 945, 1662
XmnI GAANNNNTTC 2 cut(s) 81, 102
XspI CTAG 5 cut(s) 461, 581, 699, 804, 908
ZraI GACGTC 1 cut(s) 842
Zsp2I ATGCAT 2 cut(s) 788, 1456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.