Rroxscaffold_1G00032370

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002321
Physical Location & Seq
Forward (+)
46311507 .. 46316228
4722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00032370.1

Sequence Viewer

Length: 855 bp
ATGGCGATTGGGAACATTGAGAACAGCATTGCTGAGCTGCAGGACAAGTTAAAGCCATTGGACGAGGATATGAAGATTGGGAATTTCGGCCCTTCGTTGGAAGAAGATGGCATTATTCTTCCAGTCACCTCTTTCTCTACTTTCTTTAAGAAAAATGAAGAAATATATCTGCAAAACGCTTGGCCTATGGTGAAATCTGCTTTAGAAGACTATGGTATTTCGTGCGCCCTGAATCTGGTTGAGGGTTCCATGACAGTTTCAACAACAACAAAGACTAGGGACCCAGATATTATTGAAAAGGCCAAGGAGCTTACTGAACTTTTGTCATTATCTGTTCCCGCAGATGAGGAAATAAGAATACTGGATGCCAAAATGAAATGTGATTTCCTTGAGATTGGAACAAAGAGAGGTGGGCTATGCGTAAAATATGGGATCGCCATGGAGCAATTTCGTGAACGGTGGGAACGTTTCCTAGGCCCCAAGAACACCGGCTTGAAGGTGTACAAAGTGATCCAACACAAACTTACGAAGAAGCTATTTGATCAAGGTGGATATAGATCTATCATGGAGGCATCAGTATTCATGAAAACCTCATTTGATTTTCATGAACATCTATCTACAAAGTTTGTGGAAGCACTGCATCAGATACAAGATTCAGATATAGAGGCAATTGCAGATAAGTTCAGGTCTTTGGATTCTCTAGAGGTGCATGTTAATGCAGCAGAAACCCTTTGTGCTATAACAAGGCTTGCTCCACCTGGACTTGCTACTAAAATGTGCAATCCGAGGTGTCATTTTCTTACGCACTCTACAATAATGTACAATATATGTACTTCTGCTCTAGCAATCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

284

Amino Acids

31.91

Weight (kDa)

5.59

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_KRR1_1st PF17903 44 - 122 3.5e-18 Krr1 KH1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 339
AclI AACGTT 1 cut(s) 466
AclWI GGATC 2 cut(s) 440, 505
AcsI RAATTY 1 cut(s) 82
AfaI GTAC 3 cut(s) 503, 821, 832
AfiI CCNNNNNNNGG 2 cut(s) 97, 235
AgsI TTSAA 3 cut(s) 261, 296, 496
AjnI CCWGG 1 cut(s) 757
AluBI AGCT 3 cut(s) 37, 310, 535
AluI AGCT 3 cut(s) 37, 310, 535
AlwI GGATC 2 cut(s) 440, 505
AoxI GGCC 4 cut(s) 88, 182, 300, 475
ApeKI GCWGC 2 cut(s) 37, 719
ApoI RAATTY 1 cut(s) 82
AspA2I CCTAGG 1 cut(s) 472
AspLEI GCGC 1 cut(s) 227
AspS9I GGNCC 3 cut(s) 89, 280, 476
AsuHPI GGTGA 2 cut(s) 118, 202
AvaII GGWCC 1 cut(s) 280
AvrII CCTAGG 1 cut(s) 472
BbsI GAAGAC 1 cut(s) 213
BbvI GCAGC 2 cut(s) 24, 731
BccI CCATC 1 cut(s) 101
BciT130I CCWGG 1 cut(s) 759
BclI TGATCA 1 cut(s) 541
BfaI CTAG 4 cut(s) 276, 473, 701, 842
BfmI CTRYAG 1 cut(s) 38
BglII AGATCT 1 cut(s) 557
BisI GCNGC 2 cut(s) 38, 720
BlnI CCTAGG 1 cut(s) 472
BlpI GCTNAGC 1 cut(s) 33
BlsI GCNGC 2 cut(s) 39, 721
Bme1390I CCNGG 1 cut(s) 759
Bme18I GGWCC 1 cut(s) 280
BmgT120I GGNCC 3 cut(s) 89, 280, 476
BmiI GGNNCC 4 cut(s) 247, 281, 282, 478
BmrFI CCNGG 1 cut(s) 759
BmsI GCATC 3 cut(s) 355, 581, 649
BpiI GAAGAC 1 cut(s) 213
Bpu1102I GCTNAGC 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 410
BsaBI GATNNNNATC 1 cut(s) 556
BsaJI CCNNGG 4 cut(s) 303, 438, 472, 785
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 235
Bse118I RCCGGY 1 cut(s) 488
Bse1I ACTGG 2 cut(s) 122, 366
Bse3DI GCAATG 1 cut(s) 27
Bse8I GATNNNNATC 1 cut(s) 556
BseBI CCWGG 1 cut(s) 759
BseDI CCNNGG 4 cut(s) 303, 438, 472, 785
BseGI GGATG 1 cut(s) 370
BseJI GATNNNNATC 1 cut(s) 556
BseLI CCNNNNNNNGG 2 cut(s) 97, 235
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 1 cut(s) 24
BseNI ACTGG 2 cut(s) 122, 366
BseXI GCAGC 2 cut(s) 24, 731
BshFI GGCC 4 cut(s) 90, 184, 302, 477
BsiSI CCGG 1 cut(s) 489
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 2 cut(s) 97, 235
BsmFI GGGAC 1 cut(s) 293
BsnI GGCC 4 cut(s) 90, 184, 302, 477
Bsp1407I TGTACA 2 cut(s) 501, 819
Bsp143I GATC 4 cut(s) 432, 510, 541, 557
Bsp1720I GCTNAGC 1 cut(s) 33
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 1 cut(s) 339
BspANI GGCC 4 cut(s) 90, 184, 302, 477
BspCNI CTCAG 1 cut(s) 25
BspHI TCATGA 2 cut(s) 582, 604
BspLI GGNNCC 4 cut(s) 247, 281, 282, 478
BspMAI CTGCAG 1 cut(s) 42
BspPI GGATC 2 cut(s) 440, 505
BsrDI GCAATG 1 cut(s) 27
BsrFI RCCGGY 1 cut(s) 488
BsrGI TGTACA 2 cut(s) 501, 819
BsrI ACTGG 2 cut(s) 122, 366
BssAI RCCGGY 1 cut(s) 488
BssECI CCNNGG 4 cut(s) 303, 438, 472, 785
BssMI GATC 4 cut(s) 432, 510, 541, 557
BssT1I CCWWGG 3 cut(s) 303, 438, 472
Bst2UI CCWGG 1 cut(s) 759
Bst4CI ACNGT 2 cut(s) 256, 459
BstAUI TGTACA 2 cut(s) 501, 819
BstC8I GCNNGC 1 cut(s) 750
BstDEI CTNAG 1 cut(s) 33
BstDSI CCRYGG 1 cut(s) 438
BstF5I GGATG 1 cut(s) 370
BstHHI GCGC 1 cut(s) 227
BstKTI GATC 4 cut(s) 435, 513, 544, 560
BstMBI GATC 4 cut(s) 432, 510, 541, 557
BstNI CCWGG 1 cut(s) 759
BstNSI RCATGY 1 cut(s) 713
BstSCI CCNGG 1 cut(s) 757
BstSFI CTRYAG 1 cut(s) 38
BstV1I GCAGC 2 cut(s) 24, 731
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 557
BstYI RGATCY 1 cut(s) 557
BsuRI GGCC 4 cut(s) 90, 184, 302, 477
BtgI CCRYGG 1 cut(s) 438
BtsCI GGATG 1 cut(s) 370
BtsI GCAGTG 1 cut(s) 635
BtsIMutI CAGTG 1 cut(s) 635
Cac8I GCNNGC 1 cut(s) 750
CciI TCATGA 2 cut(s) 582, 604
CfoI GCGC 1 cut(s) 227
Cfr10I RCCGGY 1 cut(s) 488
Cfr13I GGNCC 3 cut(s) 89, 280, 476
Csp6I GTAC 3 cut(s) 502, 820, 831
CspCI CAANNNNNGTGG 2 cut(s) 609, 644
CviAII CATG 6 cut(s) 250, 439, 565, 583, 605, 710
CviQI GTAC 3 cut(s) 502, 820, 831
DdeI CTNAG 1 cut(s) 33
DpnI GATC 4 cut(s) 434, 512, 543, 559
DpnII GATC 4 cut(s) 432, 510, 541, 557
Eco130I CCWWGG 3 cut(s) 303, 438, 472
Eco47I GGWCC 1 cut(s) 280
EcoO109I RGGNCCY 2 cut(s) 280, 476
EcoRII CCWGG 1 cut(s) 757
EcoT14I CCWWGG 3 cut(s) 303, 438, 472
ErhI CCWWGG 3 cut(s) 303, 438, 472
FaeI CATG 6 cut(s) 253, 442, 568, 586, 608, 713
FaqI GGGAC 1 cut(s) 293
FatI CATG 6 cut(s) 249, 438, 564, 582, 604, 709
FauI CCCGC 1 cut(s) 346
FbaI TGATCA 1 cut(s) 541
Fnu4HI GCNGC 2 cut(s) 38, 720
FokI GGATG 1 cut(s) 377
Fsp4HI GCNGC 2 cut(s) 38, 720
FspBI CTAG 4 cut(s) 276, 473, 701, 842
GlaI GCGC 1 cut(s) 226
GluI GCNGC 2 cut(s) 38, 720
HaeIII GGCC 4 cut(s) 90, 184, 302, 477
HapII CCGG 1 cut(s) 489
HhaI GCGC 1 cut(s) 227
Hin1II CATG 6 cut(s) 253, 442, 568, 586, 608, 713
Hin6I GCGC 1 cut(s) 225
HinP1I GCGC 1 cut(s) 225
HinfI GANTC 3 cut(s) 232, 653, 695
HpaII CCGG 1 cut(s) 489
HphI GGTGA 2 cut(s) 118, 202
Hpy166II GTNNAC 2 cut(s) 455, 502
Hpy188I TCNGA 3 cut(s) 645, 658, 786
Hpy188III TCNNGA 4 cut(s) 452, 583, 605, 701
Hpy8I GTNNAC 2 cut(s) 455, 502
HpyAV CCTTC 2 cut(s) 102, 490
HpyCH4III ACNGT 2 cut(s) 256, 459
HpyCH4IV ACGT 1 cut(s) 466
HpyCH4V TGCA 7 cut(s) 40, 172, 640, 674, 709, 719, 780
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 1 cut(s) 466
Hsp92II CATG 6 cut(s) 253, 442, 568, 586, 608, 713
HspAI GCGC 1 cut(s) 225
KflI GGGWCCC 1 cut(s) 280
Ksp22I TGATCA 1 cut(s) 541
Kzo9I GATC 4 cut(s) 432, 510, 541, 557
LmnI GCTCC 3 cut(s) 307, 442, 757
Lsp1109I GCAGC 2 cut(s) 24, 731
LweI GCATC 3 cut(s) 355, 581, 649
MaeI CTAG 4 cut(s) 276, 473, 701, 842
MaeII ACGT 1 cut(s) 466
MaeIII GTNAC 1 cut(s) 124
MalI GATC 4 cut(s) 434, 512, 543, 559
MboI GATC 4 cut(s) 432, 510, 541, 557
MboII GAAGA 7 cut(s) 85, 110, 113, 116, 170, 218, 541
MfeI CAATTG 1 cut(s) 669
MflI RGATCY 1 cut(s) 557
MluCI AATT 3 cut(s) 82, 446, 669
MmeI TCCRAC 2 cut(s) 78, 538
MseI TTAA 3 cut(s) 50, 147, 714
MslI CAYNNNNRTG 1 cut(s) 714
MspI CCGG 1 cut(s) 489
MspR9I CCNGG 1 cut(s) 759
MunI CAATTG 1 cut(s) 669
MvaI CCWGG 1 cut(s) 759
NcoI CCATGG 1 cut(s) 438
NdeII GATC 4 cut(s) 432, 510, 541, 557
NlaIII CATG 6 cut(s) 253, 442, 568, 586, 608, 713
NlaIV GGNNCC 4 cut(s) 247, 281, 282, 478
NmuCI GTSAC 1 cut(s) 124
NspI RCATGY 1 cut(s) 713
PagI TCATGA 2 cut(s) 582, 604
PcsI WCGNNNNNNNCGW 1 cut(s) 463
PfeI GAWTC 3 cut(s) 232, 653, 695
PkrI GCNGC 2 cut(s) 39, 721
PpuMI RGGWCCY 1 cut(s) 280
Psp1406I AACGTT 1 cut(s) 466
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 757
PspGI CCWGG 1 cut(s) 757
PspN4I GGNNCC 4 cut(s) 247, 281, 282, 478
PspPI GGNCC 3 cut(s) 89, 280, 476
PspPPI RGGWCCY 1 cut(s) 280
PstI CTGCAG 1 cut(s) 42
PsuI RGATCY 1 cut(s) 557
RsaI GTAC 3 cut(s) 503, 821, 832
RsaNI GTAC 3 cut(s) 502, 820, 831
RseI CAYNNNNRTG 1 cut(s) 714
SaqAI TTAA 3 cut(s) 50, 147, 714
SatI GCNGC 2 cut(s) 38, 720
Sau3AI GATC 4 cut(s) 432, 510, 541, 557
Sau96I GGNCC 3 cut(s) 89, 280, 476
ScrFI CCNGG 1 cut(s) 759
SfaNI GCATC 3 cut(s) 355, 581, 649
SfcI CTRYAG 1 cut(s) 38
SinI GGWCC 1 cut(s) 280
SmiMI CAYNNNNRTG 1 cut(s) 714
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 3 cut(s) 82, 446, 669
SsiI CCGC 1 cut(s) 339
SspMI CTAG 4 cut(s) 276, 473, 701, 842
StyD4I CCNGG 1 cut(s) 757
StyI CCWWGG 3 cut(s) 303, 438, 472
TaaI ACNGT 2 cut(s) 256, 459
TaiI ACGT 1 cut(s) 469
TasI AATT 3 cut(s) 82, 446, 669
TatI WGTACW 3 cut(s) 501, 819, 830
TfiI GAWTC 3 cut(s) 232, 653, 695
Tru1I TTAA 3 cut(s) 50, 147, 714
Tru9I TTAA 3 cut(s) 50, 147, 714
TscAI CASTG 1 cut(s) 642
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 2 cut(s) 37, 719
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 7 cut(s) 86, 171, 389, 571, 593, 599, 621
TspRI CASTG 1 cut(s) 642
VpaK11BI GGWCC 1 cut(s) 280
XapI RAATTY 1 cut(s) 82
XbaI TCTAGA 1 cut(s) 700
XceI RCATGY 1 cut(s) 713
XmaJI CCTAGG 1 cut(s) 472
XspI CTAG 4 cut(s) 276, 473, 701, 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.