RchiOBHm_Chr5g0049281

Serine threonine-protein phosphatase 6 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002321
Physical Location & Seq
Reverse (-)
46823778 .. 46828498
4721 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32695

Sequence Viewer

Length: 1314 bp
ATGGAAAAAATAAATAAAAATACACACGAGTTATATTCATGCAGCCTAAGGGCTTCTTGGTTTATTTTCCCTATTAACATATTTGGACTGTCAACTTGTGGGATAATTCTGAGACGATTAATGCCTTGTTATCTGGGAGATGATGCTGCGATGTGTTGCTTGCTAAAAAGTAAAAAACTCTTATCTAGTCTCGTGGTAAAGTTCTACTTATTAATACAACACTCATATGGTAAACTCTCTTACAAATTGTCAAATTCCACTCCACCTACTCCCCATGGAAAATTATGTCCAAGTCTTGTCCAAAGTCGGGGATCACAGCAAGGGTCAAATTCAATGGAACATGGGAGGAACTCTCAGTTTTCACTGAGATTGAGAATGGTGTCAGACCTTACCAAAATGGATTCTCTAGAGGTGCATGTTAATGCAGCAGAAACCCTTTGTGCTATAACAAGGCTTGCTCCACCTGGACTTGCTACTAAAATCTGTAATCCGAGTTTTATACAAAGATTGCTTCAGCATGCTATGGAGGACTCTCAACGAAAATCTGTTCTCACTAGCTCATTATCAGTTTGCATCTCTTTGTTAGACCGTGAGAGGGAAGCTTTGAGAACAAATGCATGTACTGGTGAACACCAGCCTGCTCATGGATCTACAATTACTGTTAATAACGAGATTGTTGTGGCCATGCTCAACAGCCTAGGTGATTTGCTGAAACTTTTAGATGTTTCATCGGCGGACAATATGTTGTTAACTACATATGGCAAGTTACAGCCACCGCTTGGAAAACATCGTTTGAAGATTGTAGAGTTCATTTCAGTATTACTGTCAGTTCGTAGTCAAGCTGCTGAGAAGGAATTGATTCGTTTTGCAGCAATACAAAAAGTCTTAGACTTATTTTTTAAGTACCCATATAACAATTTTTTGCATCACCACATAGAGAACATAATCATATCATGTTTGGAAAGCAAAAATGGGCCTCTTGTACAACATATTCTTCGTGAATGTGATCTTGTTGGAAAAATACTTGAAGCAGAAAAGAATTTCACACTGGCCGCTGATTCATTTATGCCAACAATTCCTGCTAAGGGTCGATCCCCACCCAGGAACGGCAATATTGGACATTTGACATGTATATCCAACAAAATCATTCAACTGGGAAACAACAATAGGGAGATTCAGGCATATCTGCAGGAAAACAGTGTCTGGACTGAATGGCATACAGAAGTCCTATTAAAGCGTAATGCAGTGGAGAATGTCTCCCAGTGGGCGTGCGGGCGGCCTATATCATCTCTGGATCGTGGACGCTCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAPS

Protein Analysis

437

Amino Acids

48.82

Weight (kDa)

9.12

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAPS PF04499 125 - 419 6.8e-48 SIT4 phosphatase-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 779
AciI CCGC 5 cut(s) 734, 776, 1053, 1272, 1276
AclWI GGATC 4 cut(s) 319, 655, 1086, 1302
AcoI YGGCCR 2 cut(s) 681, 1050
AcsI RAATTY 3 cut(s) 253, 328, 1039
AcuI CTGAAG 1 cut(s) 497
AfaI GTAC 3 cut(s) 622, 905, 984
AfiI CCNNNNNNNGG 7 cut(s) 307, 595, 644, 779, 1085, 1101, 1106
AflIII ACRYGT 1 cut(s) 1127
AgsI TTSAA 4 cut(s) 333, 796, 1028, 1151
AjnI CCWGG 2 cut(s) 463, 1100
AluBI AGCT 4 cut(s) 558, 602, 842, 1311
AluI AGCT 4 cut(s) 558, 602, 842, 1311
Alw26I GTCTC 3 cut(s) 106, 194, 1261
AlwI GGATC 4 cut(s) 319, 655, 1086, 1302
AlwNI CAGNNNCTG 1 cut(s) 1203
AoxI GGCC 4 cut(s) 681, 974, 1050, 1277
ApeKI GCWGC 5 cut(s) 42, 146, 425, 842, 869
ApoI RAATTY 3 cut(s) 253, 328, 1039
AseI ATTAAT 2 cut(s) 119, 212
Asp700I GAANNNNTTC 1 cut(s) 858
AspA2I CCTAGG 1 cut(s) 697
AspS9I GGNCC 1 cut(s) 974
AsuHPI GGTGA 3 cut(s) 638, 713, 920
AvrII CCTAGG 1 cut(s) 697
AxyI CCTNAGG 1 cut(s) 47
BalI TGGCCA 1 cut(s) 683
BauI CACGAG 2 cut(s) 26, 191
BbvI GCAGC 5 cut(s) 54, 133, 437, 829, 881
BceAI ACGGC 1 cut(s) 1123
BciT130I CCWGG 2 cut(s) 465, 1102
BcoDI GTCTC 3 cut(s) 106, 194, 1261
BfaI CTAG 5 cut(s) 186, 407, 555, 698, 1312
BfmI CTRYAG 1 cut(s) 1187
BisI GCNGC 7 cut(s) 43, 147, 426, 843, 870, 1053, 1277
BlnI CCTAGG 1 cut(s) 697
BlsI GCNGC 7 cut(s) 44, 148, 427, 844, 871, 1054, 1278
Bme1390I CCNGG 2 cut(s) 465, 1102
BmgT120I GGNCC 1 cut(s) 974
BmrFI CCNGG 2 cut(s) 465, 1102
BmrI ACTGGG 2 cut(s) 1163, 1255
BmsI GCATC 3 cut(s) 133, 582, 934
BmuI ACTGGG 2 cut(s) 1163, 1255
BplI GAGNNNNNCTC 4 cut(s) 337, 369, 1241, 1273
Bpu10I CCTNAGC 1 cut(s) 1083
BpuEI CTTGAG 1 cut(s) 1291
BsaJI CCNNGG 3 cut(s) 274, 697, 1100
Bsc4I CCNNNNNNNGG 7 cut(s) 307, 595, 644, 779, 1085, 1101, 1106
Bse1I ACTGG 4 cut(s) 628, 1053, 1158, 1261
Bse21I CCTNAGG 1 cut(s) 47
BseBI CCWGG 2 cut(s) 465, 1102
BseDI CCNNGG 3 cut(s) 274, 697, 1100
BseLI CCNNNNNNNGG 7 cut(s) 307, 595, 644, 779, 1085, 1101, 1106
BseMII CTCAG 4 cut(s) 101, 356, 368, 837
BseNI ACTGG 4 cut(s) 628, 1053, 1158, 1261
BseXI GCAGC 5 cut(s) 54, 133, 437, 829, 881
BshFI GGCC 4 cut(s) 683, 976, 1052, 1279
BslI CCNNNNNNNGG 7 cut(s) 307, 595, 644, 779, 1085, 1101, 1106
BsmAI GTCTC 3 cut(s) 106, 194, 1261
BsmBI CGTCTC 1 cut(s) 106
BsnI GGCC 4 cut(s) 683, 976, 1052, 1279
Bsp1407I TGTACA 1 cut(s) 982
Bsp143I GATC 5 cut(s) 311, 647, 1006, 1091, 1294
Bsp19I CCATGG 1 cut(s) 274
BspACI CCGC 5 cut(s) 734, 776, 1053, 1272, 1276
BspANI GGCC 4 cut(s) 683, 976, 1052, 1279
BspCNI CTCAG 4 cut(s) 102, 357, 367, 838
BspMAI CTGCAG 1 cut(s) 1191
BspPI GGATC 4 cut(s) 319, 655, 1086, 1302
BsrGI TGTACA 1 cut(s) 982
BsrI ACTGG 4 cut(s) 628, 1053, 1158, 1261
BssECI CCNNGG 3 cut(s) 274, 697, 1100
BssMI GATC 5 cut(s) 311, 647, 1006, 1091, 1294
BssSI CACGAG 2 cut(s) 26, 191
BssT1I CCWWGG 2 cut(s) 274, 697
Bst2BI CACGAG 2 cut(s) 26, 191
Bst2UI CCWGG 2 cut(s) 465, 1102
Bst4CI ACNGT 5 cut(s) 90, 590, 661, 825, 1199
BstAUI TGTACA 1 cut(s) 982
BstC8I GCNNGC 6 cut(s) 161, 456, 519, 639, 1270, 1274
BstDEI CTNAG 7 cut(s) 47, 110, 354, 365, 846, 886, 1083
BstDSI CCRYGG 1 cut(s) 274
BstKTI GATC 5 cut(s) 314, 650, 1009, 1094, 1297
BstMAI GTCTC 3 cut(s) 106, 194, 1261
BstMBI GATC 5 cut(s) 311, 647, 1006, 1091, 1294
BstNI CCWGG 2 cut(s) 465, 1102
BstNSI RCATGY 4 cut(s) 419, 521, 621, 1131
BstSCI CCNGG 2 cut(s) 463, 1100
BstSFI CTRYAG 1 cut(s) 1187
BstV1I GCAGC 5 cut(s) 54, 133, 437, 829, 881
BstX2I RGATCY 1 cut(s) 647
BstYI RGATCY 1 cut(s) 647
Bsu36I CCTNAGG 1 cut(s) 47
BsuRI GGCC 4 cut(s) 683, 976, 1052, 1279
BtgI CCRYGG 1 cut(s) 274
BtgZI GCGATG 1 cut(s) 164
BtsI GCAGTG 1 cut(s) 1251
BtsIMutI CAGTG 5 cut(s) 362, 1046, 1204, 1251, 1268
Cac8I GCNNGC 6 cut(s) 161, 456, 519, 639, 1270, 1274
CaiI CAGNNNCTG 1 cut(s) 1203
Cfr13I GGNCC 1 cut(s) 974
Csp6I GTAC 3 cut(s) 621, 904, 983
CviQI GTAC 3 cut(s) 621, 904, 983
DdeI CTNAG 7 cut(s) 47, 110, 354, 365, 846, 886, 1083
DpnI GATC 5 cut(s) 313, 649, 1008, 1093, 1296
DpnII GATC 5 cut(s) 311, 647, 1006, 1091, 1294
EaeI YGGCCR 2 cut(s) 681, 1050
EciI GGCGGA 1 cut(s) 749
Eco130I CCWWGG 2 cut(s) 274, 697
Eco57I CTGAAG 1 cut(s) 497
Eco81I CCTNAGG 1 cut(s) 47
EcoRII CCWGG 2 cut(s) 463, 1100
EcoT14I CCWWGG 2 cut(s) 274, 697
EcoT22I ATGCAT 1 cut(s) 619
ErhI CCWWGG 2 cut(s) 274, 697
Esp3I CGTCTC 1 cut(s) 106
FalI AAGNNNNNCTT 4 cut(s) 40, 72, 191, 223
FauI CCCGC 1 cut(s) 1265
FauNDI CATATG 2 cut(s) 226, 757
Fnu4HI GCNGC 7 cut(s) 43, 147, 426, 843, 870, 1053, 1277
Fsp4HI GCNGC 7 cut(s) 43, 147, 426, 843, 870, 1053, 1277
FspBI CTAG 5 cut(s) 186, 407, 555, 698, 1312
GluI GCNGC 7 cut(s) 43, 147, 426, 843, 870, 1053, 1277
HaeIII GGCC 4 cut(s) 683, 976, 1052, 1279
HincII GTYRAC 2 cut(s) 93, 750
HindII GTYRAC 2 cut(s) 93, 750
HindIII AAGCTT 1 cut(s) 600
HinfI GANTC 5 cut(s) 401, 530, 859, 1058, 1174
HpaI GTTAAC 1 cut(s) 750
HphI GGTGA 3 cut(s) 638, 713, 920
Hpy166II GTNNAC 5 cut(s) 93, 233, 629, 750, 1301
Hpy188I TCNGA 3 cut(s) 111, 385, 492
Hpy188III TCNNGA 4 cut(s) 407, 998, 1204, 1292
Hpy8I GTNNAC 5 cut(s) 93, 233, 629, 750, 1301
HpyAV CCTTC 1 cut(s) 844
HpyCH4III ACNGT 5 cut(s) 90, 590, 661, 825, 1199
HpyCH4V TGCA 9 cut(s) 42, 415, 425, 573, 617, 869, 925, 1189, 1244
HpyF3I CTNAG 7 cut(s) 47, 110, 354, 365, 846, 886, 1083
KspAI GTTAAC 1 cut(s) 750
Kzo9I GATC 5 cut(s) 311, 647, 1006, 1091, 1294
LmnI GCTCC 1 cut(s) 463
Lsp1109I GCAGC 5 cut(s) 54, 133, 437, 829, 881
LweI GCATC 3 cut(s) 133, 582, 934
MaeI CTAG 5 cut(s) 186, 407, 555, 698, 1312
MaeIII GTNAC 1 cut(s) 765
MalI GATC 5 cut(s) 313, 649, 1008, 1093, 1296
MboI GATC 5 cut(s) 311, 647, 1006, 1091, 1294
MboII GAAGA 2 cut(s) 808, 986
MflI RGATCY 1 cut(s) 647
MlsI TGGCCA 1 cut(s) 683
MluNI TGGCCA 1 cut(s) 683
MlyI GAGTC 1 cut(s) 524
MmeI TCCRAC 2 cut(s) 994, 1161
MnlI CCTC 5 cut(s) 339, 403, 520, 588, 987
Mox20I TGGCCA 1 cut(s) 683
Mph1103I ATGCAT 1 cut(s) 619
MroXI GAANNNNTTC 1 cut(s) 858
MscI TGGCCA 1 cut(s) 683
MseI TTAA 8 cut(s) 75, 119, 212, 420, 663, 749, 900, 1232
MslI CAYNNNNRTG 2 cut(s) 225, 420
Msp20I TGGCCA 1 cut(s) 683
MspA1I CMGCKG 1 cut(s) 1055
MspR9I CCNGG 2 cut(s) 465, 1102
MvaI CCWGG 2 cut(s) 465, 1102
NcoI CCATGG 1 cut(s) 274
NdeI CATATG 2 cut(s) 226, 757
NdeII GATC 5 cut(s) 311, 647, 1006, 1091, 1294
NsiI ATGCAT 1 cut(s) 619
NspI RCATGY 4 cut(s) 419, 521, 621, 1131
PaeI GCATGC 1 cut(s) 521
PciI ACATGT 1 cut(s) 1127
PdmI GAANNNNTTC 1 cut(s) 858
PfeI GAWTC 4 cut(s) 401, 859, 1058, 1174
PflMI CCANNNNNTGG 1 cut(s) 779
PkrI GCNGC 7 cut(s) 44, 148, 427, 844, 871, 1054, 1278
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
PscI ACATGT 1 cut(s) 1127
PshBI ATTAAT 2 cut(s) 119, 212
Psp6I CCWGG 2 cut(s) 463, 1100
PspGI CCWGG 2 cut(s) 463, 1100
PspPI GGNCC 1 cut(s) 974
PstI CTGCAG 1 cut(s) 1191
PstNI CAGNNNCTG 1 cut(s) 1203
PsuI RGATCY 1 cut(s) 647
RsaI GTAC 3 cut(s) 622, 905, 984
RsaNI GTAC 3 cut(s) 621, 904, 983
RseI CAYNNNNRTG 2 cut(s) 225, 420
SaqAI TTAA 8 cut(s) 75, 119, 212, 420, 663, 749, 900, 1232
SatI GCNGC 7 cut(s) 43, 147, 426, 843, 870, 1053, 1277
Sau3AI GATC 5 cut(s) 311, 647, 1006, 1091, 1294
Sau96I GGNCC 1 cut(s) 974
SchI GAGTC 1 cut(s) 524
ScrFI CCNGG 2 cut(s) 465, 1102
SetI ASST 9 cut(s) 268, 390, 414, 466, 560, 604, 703, 844, 1313
SfaNI GCATC 3 cut(s) 133, 582, 934
SfcI CTRYAG 1 cut(s) 1187
SmiMI CAYNNNNRTG 2 cut(s) 225, 420
SmlI CTYRAG 1 cut(s) 1306
SmoI CTYRAG 1 cut(s) 1306
SphI GCATGC 1 cut(s) 521
SsiI CCGC 5 cut(s) 734, 776, 1053, 1272, 1276
SspI AATATT 1 cut(s) 1114
SspMI CTAG 5 cut(s) 186, 407, 555, 698, 1312
StyD4I CCNGG 2 cut(s) 463, 1100
StyI CCWWGG 2 cut(s) 274, 697
TaaI ACNGT 5 cut(s) 90, 590, 661, 825, 1199
TaqI TCGA 1 cut(s) 1090
TatI WGTACW 2 cut(s) 620, 982
TauI GCSGC 2 cut(s) 1055, 1279
TfiI GAWTC 4 cut(s) 401, 859, 1058, 1174
Tru1I TTAA 8 cut(s) 75, 119, 212, 420, 663, 749, 900, 1232
Tru9I TTAA 8 cut(s) 75, 119, 212, 420, 663, 749, 900, 1232
TscAI CASTG 5 cut(s) 369, 1053, 1204, 1251, 1268
TseI GCWGC 5 cut(s) 42, 146, 425, 842, 869
TspDTI ATGAA 4 cut(s) 27, 717, 799, 1050
TspRI CASTG 5 cut(s) 369, 1053, 1204, 1251, 1268
Van91I CCANNNNNTGG 1 cut(s) 779
VspI ATTAAT 2 cut(s) 119, 212
XapI RAATTY 3 cut(s) 253, 328, 1039
XbaI TCTAGA 1 cut(s) 406
XceI RCATGY 4 cut(s) 419, 521, 621, 1131
XcmI CCANNNNNNNNNTGG 1 cut(s) 641
XmaJI CCTAGG 1 cut(s) 697
XmnI GAANNNNTTC 1 cut(s) 858
XspI CTAG 5 cut(s) 186, 407, 555, 698, 1312
Zsp2I ATGCAT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.