Rh5AG324000

Serine threonine-protein phosphatase 6 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002321
Physical Location & Seq
Reverse (-)
48459151 .. 48476620
17470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG324000.1

Sequence Viewer

Length: 1536 bp
ATGGAAAAAATAAATAAAAATACACGAGTTATATTCATGCAGCCTAAGGGCTTCTTGCCTTCTTGGTTTATTTTCCCTATTAACATATTTGGACTGTCAACTTGTGGGATAATTCTGAGACAATTAATGCCTTGTTATCTGGGAGATGATGCTGTGATGTGTTGCTTGCTAAAAAGTAAAAAACTCTTATCTAGTCTGGTGGTAAAGTTCTACTTATTAATACAACACTCATATGGTAAACTCTCTTACAAATTGTCAAATTCCACTCCACCTACTCCCCATGGAAAATTATGTCCAAGTCTTGTCCAAAGTCGGGGATCACAGCAAGGGTCAAATTCAATGGAACATGGGAGGAACTCTCAGTTTTCACTGAGATTGAGAATGGTGTCAGACCTTACCAAAATGGATTCTCTAGAGGTGCATGTTAATGCAGCAGAAACCCTTTGTGCTATAACAAGGCTTGCTCCACCTGGACTTGCTACTAAAATCTGTAATCCGAGTTTTATACAAAGATTGCTTCAGCATGCTATGGAGGACTCTCAGCGAAATTCTGTTCTCACTAGCTCATTATCAGTTTGCATCTCTTTGTTAGACCGTGAGAGGGAAGCTTTGAGAACAAATACATGTACTGGTGAACACCAGCCTGCTCATGGACCTACAATTACTGTTAATAACGAGATTGTTGTGGCCATGCTCAACAGCCTAGGTGATTTGCTGAAACTTTTAGATGTTTCATCGGCAGACAATATGTTGTTAACTACATATGGCAAGTTACAGCCACCTCTTGGAAAACATCGTTTGAAGATTGTAGAGTTCATTTCAGTATTACTGTCAGTTCGTAGTCAAGCTGCTGAGAAGGAATTGATTCGTATTGCAGCAATACAAAAAGTCTTAGACTTGTTTTTTAAGTACCCATGTAACAATTTTTTGCATCACCACATAGAGAACATAATCATATCATGTTTGGAAAGCAAAAATGGGCCTCTTGTACAACATCTTCTTCATGAATGTGATCTTGTTGGAAAAATACTTGAAGCAGAAAAGAATTTCACACTGGCCGCTGATTCATTTATGCCAACAATTCCTGCTAAGGGTAGATCCCCACCCAGGAACGGCAATATTGGACATTTGACATGTATATCCAACAAAATCATTCAACTGGGAAACAACAATAGGGAGATTCAGGCATATCTGCAGGAAAACAGTGTCTGGACTGAATGGCATACAGAAGTCCTATTAAAGCGTAATGCAGTGGAGAATGTCTCCCAGTGGGCGTGCGGGCGGCCTATATCATCTCTGGATCGTGGACGCTCAAGCTATGTCTTGGCGGCCATTGCTCTCTCCCTCTTTCTCGGTCTTAAGGGTGACCCTGTGCCTTGCGAGAGGTGTGCTGGCAATGGTGGTACAAAATGTGTCTTCTGTAACAATGGTAAAATGAAGCTAGAAACAGGCTTGGTTGATTGCAAAGTGTGCAAGGGCGCAGGATTGGTACTTTGCAAGAAATGTGCAGGTTCTGGATATTCTCGACGGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAPS

Protein Analysis

511

Amino Acids

56.44

Weight (kDa)

9.15

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAPS PF04499 127 - 421 6.9e-47 SIT4 phosphatase-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1499
AccB7I CCANNNNNTGG 1 cut(s) 785
AciI CCGC 4 cut(s) 1059, 1278, 1282, 1328
AclWI GGATC 3 cut(s) 325, 1092, 1308
AcoI YGGCCR 3 cut(s) 687, 1056, 1329
AcsI RAATTY 4 cut(s) 259, 334, 547, 1045
AcuI CTGAAG 1 cut(s) 503
AfaI GTAC 5 cut(s) 628, 911, 990, 1405, 1491
AfiI CCNNNNNNNGG 7 cut(s) 313, 601, 650, 785, 1091, 1107, 1112
AflII CTTAAG 1 cut(s) 1358
AflIII ACRYGT 2 cut(s) 623, 1133
AgsI TTSAA 4 cut(s) 339, 802, 1034, 1157
AjnI CCWGG 2 cut(s) 469, 1106
AluBI AGCT 5 cut(s) 564, 608, 848, 1317, 1441
AluI AGCT 5 cut(s) 564, 608, 848, 1317, 1441
Alw26I GTCTC 2 cut(s) 112, 1267
AlwI GGATC 3 cut(s) 325, 1092, 1308
AlwNI CAGNNNCTG 2 cut(s) 1209, 1514
AoxI GGCC 5 cut(s) 687, 980, 1056, 1283, 1329
ApeKI GCWGC 4 cut(s) 40, 431, 848, 875
ApoI RAATTY 4 cut(s) 259, 334, 547, 1045
AseI ATTAAT 2 cut(s) 125, 218
Asp700I GAANNNNTTC 1 cut(s) 864
AspA2I CCTAGG 1 cut(s) 703
AspLEI GCGC 1 cut(s) 1481
AspS9I GGNCC 2 cut(s) 653, 980
AsuHPI GGTGA 4 cut(s) 644, 719, 926, 1376
AvaII GGWCC 1 cut(s) 653
AvrII CCTAGG 1 cut(s) 703
AxyI CCTNAGG 1 cut(s) 45
BalI TGGCCA 1 cut(s) 689
BarI GAAGNNNNNNTAC 2 cut(s) 981, 1013
BauI CACGAG 1 cut(s) 24
BbsI GAAGAC 1 cut(s) 1408
BbvI GCAGC 4 cut(s) 52, 443, 835, 887
BceAI ACGGC 1 cut(s) 1129
BcgI CGANNNNNNTGC 2 cut(s) 1370, 1404
BciT130I CCWGG 2 cut(s) 471, 1108
BcoDI GTCTC 2 cut(s) 112, 1267
BfaI CTAG 5 cut(s) 192, 413, 561, 704, 1442
BfmI CTRYAG 1 cut(s) 1193
BfrI CTTAAG 1 cut(s) 1358
BfuAI ACCTGC 1 cut(s) 1499
BisI GCNGC 7 cut(s) 41, 432, 849, 876, 1059, 1283, 1329
BlnI CCTAGG 1 cut(s) 703
BlsI GCNGC 7 cut(s) 42, 433, 850, 877, 1060, 1284, 1330
Bme1390I CCNGG 2 cut(s) 471, 1108
Bme18I GGWCC 1 cut(s) 653
BmgT120I GGNCC 2 cut(s) 653, 980
BmrFI CCNGG 2 cut(s) 471, 1108
BmrI ACTGGG 2 cut(s) 1169, 1261
BmsI GCATC 3 cut(s) 139, 588, 940
BmuI ACTGGG 2 cut(s) 1169, 1261
BpiI GAAGAC 1 cut(s) 1408
BplI GAGNNNNNCTC 4 cut(s) 343, 375, 1247, 1279
Bpu10I CCTNAGC 1 cut(s) 1089
BpuEI CTTGAG 1 cut(s) 1297
BsaJI CCNNGG 3 cut(s) 280, 703, 1106
Bsc4I CCNNNNNNNGG 7 cut(s) 313, 601, 650, 785, 1091, 1107, 1112
Bse1I ACTGG 4 cut(s) 634, 1059, 1164, 1267
Bse21I CCTNAGG 1 cut(s) 45
Bse3DI GCAATG 2 cut(s) 1332, 1402
BseBI CCWGG 2 cut(s) 471, 1108
BseDI CCNNGG 3 cut(s) 280, 703, 1106
BseLI CCNNNNNNNGG 7 cut(s) 313, 601, 650, 785, 1091, 1107, 1112
BseMI GCAATG 2 cut(s) 1332, 1402
BseMII CTCAG 5 cut(s) 107, 362, 374, 554, 843
BseNI ACTGG 4 cut(s) 634, 1059, 1164, 1267
BseXI GCAGC 4 cut(s) 52, 443, 835, 887
BsgI GTGCAG 1 cut(s) 1527
BshFI GGCC 5 cut(s) 689, 982, 1058, 1285, 1331
BslI CCNNNNNNNGG 7 cut(s) 313, 601, 650, 785, 1091, 1107, 1112
BsmAI GTCTC 2 cut(s) 112, 1267
BsnI GGCC 5 cut(s) 689, 982, 1058, 1285, 1331
Bsp1407I TGTACA 1 cut(s) 988
Bsp143I GATC 4 cut(s) 317, 1012, 1097, 1300
Bsp19I CCATGG 1 cut(s) 280
BspACI CCGC 4 cut(s) 1059, 1278, 1282, 1328
BspANI GGCC 5 cut(s) 689, 982, 1058, 1285, 1331
BspCNI CTCAG 5 cut(s) 108, 363, 373, 553, 844
BspHI TCATGA 1 cut(s) 1003
BspMAI CTGCAG 1 cut(s) 1197
BspMI ACCTGC 1 cut(s) 1499
BspPI GGATC 3 cut(s) 325, 1092, 1308
BspTI CTTAAG 1 cut(s) 1358
BsrDI GCAATG 2 cut(s) 1332, 1402
BsrGI TGTACA 1 cut(s) 988
BsrI ACTGG 4 cut(s) 634, 1059, 1164, 1267
BssECI CCNNGG 3 cut(s) 280, 703, 1106
BssMI GATC 4 cut(s) 317, 1012, 1097, 1300
BssSI CACGAG 1 cut(s) 24
BssT1I CCWWGG 2 cut(s) 280, 703
Bst2BI CACGAG 1 cut(s) 24
Bst2UI CCWGG 2 cut(s) 471, 1108
Bst4CI ACNGT 6 cut(s) 96, 596, 667, 831, 1205, 1530
BstAFI CTTAAG 1 cut(s) 1358
BstAPI GCANNNNNTGC 1 cut(s) 1470
BstAUI TGTACA 1 cut(s) 988
BstC8I GCNNGC 7 cut(s) 167, 462, 525, 645, 1276, 1280, 1393
BstDEI CTNAG 8 cut(s) 45, 116, 360, 371, 540, 852, 892, 1089
BstDSI CCRYGG 1 cut(s) 280
BstEII GGTNACC 1 cut(s) 1364
BstHHI GCGC 1 cut(s) 1481
BstKTI GATC 4 cut(s) 320, 1015, 1100, 1303
BstMAI GTCTC 2 cut(s) 112, 1267
BstMBI GATC 4 cut(s) 317, 1012, 1097, 1300
BstMWI GCNNNNNNNGC 2 cut(s) 1334, 1470
BstNI CCWGG 2 cut(s) 471, 1108
BstNSI RCATGY 4 cut(s) 425, 527, 627, 1137
BstPI GGTNACC 1 cut(s) 1364
BstSCI CCNGG 2 cut(s) 469, 1106
BstSFI CTRYAG 1 cut(s) 1193
BstV1I GCAGC 4 cut(s) 52, 443, 835, 887
BstV2I GAAGAC 1 cut(s) 1408
BstX2I RGATCY 1 cut(s) 1097
BstYI RGATCY 1 cut(s) 1097
Bsu36I CCTNAGG 1 cut(s) 45
BsuRI GGCC 5 cut(s) 689, 982, 1058, 1285, 1331
BtgI CCRYGG 1 cut(s) 280
BtsI GCAGTG 1 cut(s) 1257
BtsIMutI CAGTG 5 cut(s) 368, 1052, 1210, 1257, 1274
BveI ACCTGC 1 cut(s) 1499
Cac8I GCNNGC 7 cut(s) 167, 462, 525, 645, 1276, 1280, 1393
CaiI CAGNNNCTG 2 cut(s) 1209, 1514
CciI TCATGA 1 cut(s) 1003
CfoI GCGC 1 cut(s) 1481
Cfr13I GGNCC 2 cut(s) 653, 980
CseI GACGC 1 cut(s) 1317
Csp6I GTAC 5 cut(s) 627, 910, 989, 1404, 1490
CviQI GTAC 5 cut(s) 627, 910, 989, 1404, 1490
DdeI CTNAG 8 cut(s) 45, 116, 360, 371, 540, 852, 892, 1089
DpnI GATC 4 cut(s) 319, 1014, 1099, 1302
DpnII GATC 4 cut(s) 317, 1012, 1097, 1300
EaeI YGGCCR 3 cut(s) 687, 1056, 1329
Eco130I CCWWGG 2 cut(s) 280, 703
Eco47I GGWCC 1 cut(s) 653
Eco57I CTGAAG 1 cut(s) 503
Eco81I CCTNAGG 1 cut(s) 45
Eco91I GGTNACC 1 cut(s) 1364
EcoO65I GGTNACC 1 cut(s) 1364
EcoRII CCWGG 2 cut(s) 469, 1106
EcoT14I CCWWGG 2 cut(s) 280, 703
ErhI CCWWGG 2 cut(s) 280, 703
FalI AAGNNNNNCTT 4 cut(s) 38, 70, 197, 229
FauI CCCGC 1 cut(s) 1271
FauNDI CATATG 2 cut(s) 232, 763
Fnu4HI GCNGC 7 cut(s) 41, 432, 849, 876, 1059, 1283, 1329
Fsp4HI GCNGC 7 cut(s) 41, 432, 849, 876, 1059, 1283, 1329
FspBI CTAG 5 cut(s) 192, 413, 561, 704, 1442
GlaI GCGC 1 cut(s) 1480
GluI GCNGC 7 cut(s) 41, 432, 849, 876, 1059, 1283, 1329
HaeIII GGCC 5 cut(s) 689, 982, 1058, 1285, 1331
HgaI GACGC 1 cut(s) 1317
HhaI GCGC 1 cut(s) 1481
Hin6I GCGC 1 cut(s) 1479
HinP1I GCGC 1 cut(s) 1479
HincII GTYRAC 2 cut(s) 99, 756
HindII GTYRAC 2 cut(s) 99, 756
HindIII AAGCTT 1 cut(s) 606
HinfI GANTC 5 cut(s) 407, 536, 865, 1064, 1180
HpaI GTTAAC 1 cut(s) 756
HphI GGTGA 4 cut(s) 644, 719, 926, 1376
Hpy166II GTNNAC 5 cut(s) 99, 239, 635, 756, 1307
Hpy188I TCNGA 3 cut(s) 117, 391, 498
Hpy188III TCNNGA 6 cut(s) 413, 1004, 1210, 1298, 1515, 1524
Hpy8I GTNNAC 5 cut(s) 99, 239, 635, 756, 1307
Hpy99I CGWCG 1 cut(s) 1530
HpyAV CCTTC 2 cut(s) 69, 850
HpyCH4III ACNGT 6 cut(s) 96, 596, 667, 831, 1205, 1530
HpyF10VI GCNNNNNNNGC 2 cut(s) 1334, 1470
HpyF3I CTNAG 8 cut(s) 45, 116, 360, 371, 540, 852, 892, 1089
HspAI GCGC 1 cut(s) 1479
KspAI GTTAAC 1 cut(s) 756
Kzo9I GATC 4 cut(s) 317, 1012, 1097, 1300
LmnI GCTCC 1 cut(s) 469
Lsp1109I GCAGC 4 cut(s) 52, 443, 835, 887
LweI GCATC 3 cut(s) 139, 588, 940
MaeI CTAG 5 cut(s) 192, 413, 561, 704, 1442
MaeIII GTNAC 4 cut(s) 771, 917, 1364, 1421
MalI GATC 4 cut(s) 319, 1014, 1099, 1302
MboI GATC 4 cut(s) 317, 1012, 1097, 1300
MboII GAAGA 4 cut(s) 814, 989, 992, 1408
MflI RGATCY 1 cut(s) 1097
MlsI TGGCCA 1 cut(s) 689
MluNI TGGCCA 1 cut(s) 689
MlyI GAGTC 1 cut(s) 530
MmeI TCCRAC 2 cut(s) 1000, 1167
MnlI CCTC 8 cut(s) 345, 409, 526, 594, 792, 993, 1355, 1377
Mox20I TGGCCA 1 cut(s) 689
MroXI GAANNNNTTC 1 cut(s) 864
MscI TGGCCA 1 cut(s) 689
MseI TTAA 9 cut(s) 81, 125, 218, 426, 669, 755, 906, 1238, 1359
MslI CAYNNNNRTG 3 cut(s) 231, 426, 1008
Msp20I TGGCCA 1 cut(s) 689
MspA1I CMGCKG 1 cut(s) 1061
MspCI CTTAAG 1 cut(s) 1358
MspR9I CCNGG 2 cut(s) 471, 1108
MvaI CCWGG 2 cut(s) 471, 1108
MwoI GCNNNNNNNGC 2 cut(s) 1334, 1470
NcoI CCATGG 1 cut(s) 280
NdeI CATATG 2 cut(s) 232, 763
NdeII GATC 4 cut(s) 317, 1012, 1097, 1300
NmuCI GTSAC 1 cut(s) 1364
NspI RCATGY 4 cut(s) 425, 527, 627, 1137
PaeI GCATGC 1 cut(s) 527
PagI TCATGA 1 cut(s) 1003
PciI ACATGT 2 cut(s) 623, 1133
PdmI GAANNNNTTC 1 cut(s) 864
PfeI GAWTC 4 cut(s) 407, 865, 1064, 1180
PflMI CCANNNNNTGG 1 cut(s) 785
PkrI GCNGC 7 cut(s) 42, 433, 850, 877, 1060, 1284, 1330
PleI GAGTC 1 cut(s) 530
PpsI GAGTC 1 cut(s) 530
PscI ACATGT 2 cut(s) 623, 1133
PshBI ATTAAT 2 cut(s) 125, 218
Psp6I CCWGG 2 cut(s) 469, 1106
PspEI GGTNACC 1 cut(s) 1364
PspGI CCWGG 2 cut(s) 469, 1106
PspPI GGNCC 2 cut(s) 653, 980
PstI CTGCAG 1 cut(s) 1197
PstNI CAGNNNCTG 2 cut(s) 1209, 1514
PsuI RGATCY 1 cut(s) 1097
RsaI GTAC 5 cut(s) 628, 911, 990, 1405, 1491
RsaNI GTAC 5 cut(s) 627, 910, 989, 1404, 1490
RseI CAYNNNNRTG 3 cut(s) 231, 426, 1008
SaqAI TTAA 9 cut(s) 81, 125, 218, 426, 669, 755, 906, 1238, 1359
SatI GCNGC 7 cut(s) 41, 432, 849, 876, 1059, 1283, 1329
Sau3AI GATC 4 cut(s) 317, 1012, 1097, 1300
Sau96I GGNCC 2 cut(s) 653, 980
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 2 cut(s) 471, 1108
SfaNI GCATC 3 cut(s) 139, 588, 940
SfcI CTRYAG 1 cut(s) 1193
SinI GGWCC 1 cut(s) 653
SmiMI CAYNNNNRTG 3 cut(s) 231, 426, 1008
SmlI CTYRAG 2 cut(s) 1312, 1358
SmoI CTYRAG 2 cut(s) 1312, 1358
SphI GCATGC 1 cut(s) 527
SsiI CCGC 4 cut(s) 1059, 1278, 1282, 1328
SspI AATATT 1 cut(s) 1120
SspMI CTAG 5 cut(s) 192, 413, 561, 704, 1442
StyD4I CCNGG 2 cut(s) 469, 1106
StyI CCWWGG 2 cut(s) 280, 703
TaaI ACNGT 6 cut(s) 96, 596, 667, 831, 1205, 1530
TaqI TCGA 1 cut(s) 1525
TaqII GACCGA 1 cut(s) 1343
TatI WGTACW 2 cut(s) 626, 988
TauI GCSGC 3 cut(s) 1061, 1285, 1331
TfiI GAWTC 4 cut(s) 407, 865, 1064, 1180
Tru1I TTAA 9 cut(s) 81, 125, 218, 426, 669, 755, 906, 1238, 1359
Tru9I TTAA 9 cut(s) 81, 125, 218, 426, 669, 755, 906, 1238, 1359
TscAI CASTG 5 cut(s) 375, 1059, 1210, 1257, 1274
TseFI GTSAC 1 cut(s) 1364
TseI GCWGC 4 cut(s) 40, 431, 848, 875
Tsp45I GTSAC 1 cut(s) 1364
TspDTI ATGAA 7 cut(s) 25, 723, 805, 992, 1020, 1056, 1451
TspRI CASTG 5 cut(s) 375, 1059, 1210, 1257, 1274
Van91I CCANNNNNTGG 1 cut(s) 785
Vha464I CTTAAG 1 cut(s) 1358
VpaK11BI GGWCC 1 cut(s) 653
VspI ATTAAT 2 cut(s) 125, 218
XapI RAATTY 4 cut(s) 259, 334, 547, 1045
XbaI TCTAGA 1 cut(s) 412
XceI RCATGY 4 cut(s) 425, 527, 627, 1137
XcmI CCANNNNNNNNNTGG 1 cut(s) 647
XmaJI CCTAGG 1 cut(s) 703
XmnI GAANNNNTTC 1 cut(s) 864
XspI CTAG 5 cut(s) 192, 413, 561, 704, 1442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.