MD10G1253800.v1.1

Serine threonine-protein phosphatase 6 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002321
Physical Location & Seq
Forward (+)
34628075 .. 34637254
9180 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1253800.v1.1.491

Sequence Viewer

Length: 2331 bp
ATGTTTTGGCGTATGGCGGGGTTGTCCACGGCGTCTGCGGTGGAGACAATTTTGGACAAGGATCATTTCACGTTGGAAGAGCTACTTGATGAAGATGAAATTATTCAAGAATGCAAAGCTCTTAATGGCCGCCTAATCAATTTTTTGCGAGAAAGAGCCCAGGTTGAGCAACTCATCCGATACATTGTTGAAGAAGCTCCCGAGGATGCTGAAAATAGACGAACTTTCAAATTTCCTTTTATTGCATGTGAGATTTTTACTTGTGAAGTTGATATCATACTCAAAACATTGGTAGAGGATGAGGAGTTGATGAACTTGTTGTTCTCCTTTTTGGAACCAAATAATTCTCACAGCACACTCTTGGCTGGGTACTTTAGCAAGGTTGTTGTTTGCCTGCTGTTGCGCAAGGCAGTTCCTTTTCTGCAGTATATTCAGGGTCATCAAGATATTGTCAAGAAGCTAGTTGACCTGATTGGAATTACATCTATTATGGAGGTTTTGATACGTCTGGTTGGTGCTGATGAACATATGTATTCTAACTATACTGATGCAATGCACTGGATACAAGATACAGATGTGCTAGAGATGATTGTGGACAAGTTCAGCTCTTCGGATTCTCCTGAAGTGCATGCTAATGCAGCGGAGACACTTTGTGCTATAACACGGTTTGCTCCGCCTGGACTTTCTTCAAAAATCTCCAGCCCAAGCTTTATAGGAAGATTGTTTCGGCATGCTTTGGAGGATTCACGGCCAAAATCTGTTCTTGTTAACTCATTGTCGGTATGCATCTCTTTGTTAGACCCTAGGAGGCTAACCTTGGGAACATATCATATGTATGGACGTCAGATGACTCATGGATCCACAGTTACCGCTAATCCTGAGACTGTTGTGGGCATGCTGGAGAGCCTAGGTGATTTGCTGAAGCTGTTGGATGTTTCGTCGGTGGATAACATGCTGTTAACTACATATGGCAAGTTACAGCCGCCTCTTGGAAAACATCGGTTAAAGGTTGTAGAGTTCATTTCAGTATTACTTACGGTTGGCAGTGAAGCTGCTGAGAAGGAATTGATTCGTCTTGGAGCTGTACAAAGAATTTTAGACTTGTTTTTCAAGTACCCATATAACAACTTTGTACATCACCACATAGAGAACATAATTGTGTCGTGTTTGGAAAGCAAGAATGTTCCTCTTGTCGAACATCTTCTTCATGAGTGTGACCTTGTTGGAAAAATACTTCAAGCAGAAAAGAATTTCACATTGGCAGCCGACTCGGACAAGCCAACTGTTCCTGTTGAGGGTAGATCCCCACCCAGAATTGGGAACATTGGACATTTAACACGTATATCCAACAAACTCATTCAGCTAGGAAATAACAATAGCGAGATTCAGACGCTGCTGCAGGAAAACAGTGAGTGGGCGGAGTGGCAGGCAAGTGTCCTGTCAAAACGTAATGCAGTCGAGAACGTCTATCAGTGGGCCTGTGGGCGGCCAACTGCTTTACATGATCGAAATAGGGATAGTGATGATGATGACTATCAAGATAGAGATTATGATGTTGCAGCCCTTGCAAATAACTTAAGCCAGGCTTTCCGATATGGAATTTACAACAATGATGAGTTAGATGAGGGCCATGGCTCACTTGAACGAGATGACGAGGATGTCTACTTTGATGATGAATCTGCTGAAGTCGTCATATCTTCATTGCGTTTGGGAGATGACCAGGAAAGTGGTTCTCTGTTTACAAATTCCAACTGGTTTGCTTTTGAGGATGACAGGGCTGCCACTGATCACTCAACTGGCTCTCTTGCTTCTCCATCACCTCATACGGCGGAGTCTAATGTTGTCAATGGTGGCGGGGATGATGATGATGTCATTGTTGGTGAAGATGATGACTTAGTTGACACCGCAACATCTTCTCCGGAACCAGGAATAAAACCAGAAGATTCGGAAGTTGCCGTCCCAAACAATTCGTTAGAATTGGGTCCCGTTGAAACTGAAAAACCACCTGAGTGGGTTGAATGGAGGGAGACTTCAGACTCTGGGGAACCCTCTGATGCCCTAGCAAATGGTGAACTTCAAACGAAATCTGCCGATGCAGCTAAATGTTCATCATCTTCTGAGCCATTGATGAAAGATGATAAAGTTGTTACCGAGCCTCAAGCTGAATCAACGGTTGAGGGAACTTCAAAGCCGCCTGAGCTGTCAGAATCAGGCAATGAGAAGCCAAGTTCTGAGTCAACCACTTCTGATGCTGCAGCTGCCGACGTGGGAACAAAAGGCAGTCAAGAAGCGGCTCCAGGAGATAAAGAGAAAGATAAGGAAGGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAPS

Protein Analysis

777

Amino Acids

85.79

Weight (kDa)

4.52

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAPS PF04499 131 - 489 1.6e-63 SIT4 phosphatase-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1658
AatII GACGTC 1 cut(s) 844
Acc16I TGCGCA 1 cut(s) 404
AccI GTMKAC 1 cut(s) 1662
AccIII TCCGGA 1 cut(s) 1918
AclWI GGATC 4 cut(s) 69, 852, 865, 1296
AcoI YGGCCR 3 cut(s) 127, 749, 1487
AcsI RAATTY 5 cut(s) 230, 1092, 1249, 1599, 1744
AcuI CTGAAG 4 cut(s) 642, 941, 1704, 2016
AcyI GRCGYC 2 cut(s) 32, 841
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 4 cut(s) 371, 1086, 1115, 1134
AfiI CCNNNNNNNGG 6 cut(s) 989, 1295, 1316, 1317, 1485, 1925
AflII CTTAAG 1 cut(s) 1576
AflIII ACRYGT 1 cut(s) 1337
AjiI CACGTC 1 cut(s) 2266
AjnI CCWGG 6 cut(s) 159, 676, 1581, 1719, 1924, 2296
AjuI GAANNNNNNNTTGG 2 cut(s) 1241, 1273
AleI CACNNNNGTG 1 cut(s) 2008
Alw26I GTCTC 4 cut(s) 38, 638, 875, 2021
AlwI GGATC 4 cut(s) 69, 852, 865, 1296
AlwNI CAGNNNCTG 2 cut(s) 1393, 2039
Ama87I CYCGRG 1 cut(s) 200
Aor13HI TCCGGA 1 cut(s) 1918
AoxI GGCC 5 cut(s) 127, 749, 1476, 1487, 1627
ApoI RAATTY 5 cut(s) 230, 1092, 1249, 1599, 1744
Asp700I GAANNNNTTC 3 cut(s) 102, 1068, 1200
AspA2I CCTAGG 2 cut(s) 803, 907
AspLEI GCGC 1 cut(s) 405
AspS9I GGNCC 3 cut(s) 1476, 1627, 1982
AsuHPI GGTGA 5 cut(s) 923, 1130, 1809, 1892, 2081
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 1 cut(s) 1982
AvrII CCTAGG 2 cut(s) 803, 907
BaeI ACNNNNGTAYC 2 cut(s) 561, 594
BamHI GGATCC 1 cut(s) 857
BanII GRGCYC 1 cut(s) 160
BccI CCATC 1 cut(s) 1822
BceAI ACGGC 4 cut(s) 45, 764, 1842, 1940
BcgI CGANNNNNNTGC 2 cut(s) 1251, 1285
BciT130I CCWGG 6 cut(s) 161, 678, 1583, 1721, 1926, 2298
BciVI GTATCC 1 cut(s) 555
BclI TGATCA 1 cut(s) 1786
BcoDI GTCTC 4 cut(s) 38, 638, 875, 2021
BfaI CTAG 7 cut(s) 461, 581, 804, 908, 1364, 2060, 2329
BfmI CTRYAG 3 cut(s) 422, 1397, 2253
BfrI CTTAAG 1 cut(s) 1576
BfuI GTATCC 1 cut(s) 555
BlnI CCTAGG 2 cut(s) 803, 907
Bme1390I CCNGG 6 cut(s) 161, 678, 1583, 1721, 1926, 2298
Bme18I GGWCC 1 cut(s) 1982
BmeT110I CYCGRG 1 cut(s) 200
BmgBI CACGTC 1 cut(s) 2266
BmgT120I GGNCC 3 cut(s) 1476, 1627, 1982
BmiI GGNNCC 7 cut(s) 336, 859, 1923, 1983, 1984, 2046, 2295
BmrFI CCNGG 6 cut(s) 161, 678, 1583, 1721, 1926, 2298
BmsI GCATC 6 cut(s) 196, 538, 795, 2044, 2083, 2239
BpmI CTGGAG 3 cut(s) 682, 920, 2280
Bpu10I CCTNAGC 1 cut(s) 2196
BpuEI CTTGAG 1 cut(s) 2142
BsaAI YACGTR 1 cut(s) 1340
BsaBI GATNNNNATC 1 cut(s) 1533
BsaHI GRCGYC 2 cut(s) 32, 841
BsaJI CCNNGG 7 cut(s) 27, 159, 201, 803, 816, 907, 1630
BsaWI WCCGGW 1 cut(s) 1918
BsaXI ACNNNNNCTCC 2 cut(s) 1900, 1930
Bsc4I CCNNNNNNNGG 6 cut(s) 989, 1295, 1316, 1317, 1485, 1925
Bse1I ACTGG 3 cut(s) 563, 1757, 1801
Bse3DI GCAATG 3 cut(s) 558, 1700, 2221
Bse8I GATNNNNATC 1 cut(s) 1533
BseAI TCCGGA 1 cut(s) 1918
BseBI CCWGG 6 cut(s) 161, 678, 1583, 1721, 1926, 2298
BseDI CCNNGG 7 cut(s) 27, 159, 201, 803, 816, 907, 1630
BseGI GGATG 7 cut(s) 174, 211, 304, 937, 1663, 1774, 1864
BseJI GATNNNNATC 1 cut(s) 1533
BseLI CCNNNNNNNGG 6 cut(s) 989, 1295, 1316, 1317, 1485, 1925
BseMI GCAATG 3 cut(s) 558, 1700, 2221
BseMII CTCAG 6 cut(s) 870, 1047, 1998, 2109, 2187, 2223
BseNI ACTGG 3 cut(s) 563, 1757, 1801
BseRI GAGGAG 1 cut(s) 317
BseYI CCCAGC 1 cut(s) 365
BshFI GGCC 5 cut(s) 129, 751, 1478, 1489, 1629
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 1 cut(s) 1919
BslFI GGGAC 2 cut(s) 1943, 1968
BslI CCNNNNNNNGG 6 cut(s) 989, 1295, 1316, 1317, 1485, 1925
BsmAI GTCTC 4 cut(s) 38, 638, 875, 2021
BsmFI GGGAC 2 cut(s) 1943, 1968
BsmI GAATGC 1 cut(s) 116
BsnI GGCC 5 cut(s) 129, 751, 1478, 1489, 1629
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 1 cut(s) 160
Bsp13I TCCGGA 1 cut(s) 1918
Bsp1407I TGTACA 2 cut(s) 1084, 1132
Bsp143I GATC 5 cut(s) 61, 857, 1301, 1504, 1786
Bsp19I CCATGG 1 cut(s) 1630
BspANI GGCC 5 cut(s) 129, 751, 1478, 1489, 1629
BspCNI CTCAG 6 cut(s) 871, 1048, 1999, 2110, 2188, 2224
BspEI TCCGGA 1 cut(s) 1918
BspHI TCATGA 1 cut(s) 1207
BspLI GGNNCC 7 cut(s) 336, 859, 1923, 1983, 1984, 2046, 2295
BspMAI CTGCAG 3 cut(s) 426, 1401, 2257
BspPI GGATC 4 cut(s) 69, 852, 865, 1296
BspQI GCTCTTC 2 cut(s) 72, 613
BspTI CTTAAG 1 cut(s) 1576
BsrDI GCAATG 3 cut(s) 558, 1700, 2221
BsrGI TGTACA 2 cut(s) 1084, 1132
BsrI ACTGG 3 cut(s) 563, 1757, 1801
BssECI CCNNGG 7 cut(s) 27, 159, 201, 803, 816, 907, 1630
BssMI GATC 5 cut(s) 61, 857, 1301, 1504, 1786
BssNI GRCGYC 2 cut(s) 32, 841
BssT1I CCWWGG 4 cut(s) 803, 816, 907, 1630
Bst2UI CCWGG 6 cut(s) 161, 678, 1583, 1721, 1926, 2298
Bst4CI ACNGT 7 cut(s) 666, 865, 886, 1039, 1285, 1409, 2173
Bst6I CTCTTC 2 cut(s) 72, 613
BstACI GRCGYC 2 cut(s) 32, 841
BstAFI CTTAAG 1 cut(s) 1576
BstAPI GCANNNNNTGC 1 cut(s) 1565
BstAUI TGTACA 2 cut(s) 1084, 1132
BstBAI YACGTR 1 cut(s) 1340
BstC8I GCNNGC 5 cut(s) 395, 630, 732, 896, 1428
BstDEI CTNAG 7 cut(s) 879, 1056, 1894, 2007, 2118, 2196, 2232
BstDSI CCRYGG 2 cut(s) 27, 1630
BstF5I GGATG 7 cut(s) 174, 211, 304, 937, 1663, 1774, 1864
BstHHI GCGC 1 cut(s) 405
BstKTI GATC 5 cut(s) 64, 860, 1304, 1507, 1789
BstMAI GTCTC 4 cut(s) 38, 638, 875, 2021
BstMBI GATC 5 cut(s) 61, 857, 1301, 1504, 1786
BstMWI GCNNNNNNNGC 5 cut(s) 638, 1565, 2096, 2197, 2258
BstNI CCWGG 6 cut(s) 161, 678, 1583, 1721, 1926, 2298
BstNSI RCATGY 5 cut(s) 249, 632, 734, 898, 955
BstSCI CCNGG 6 cut(s) 159, 676, 1581, 1719, 1924, 2296
BstSFI CTRYAG 3 cut(s) 422, 1397, 2253
BstX2I RGATCY 2 cut(s) 857, 1301
BstXI CCANNNNNNTGG 2 cut(s) 1727, 2010
BstYI RGATCY 2 cut(s) 857, 1301
BsuI GTATCC 1 cut(s) 555
BsuRI GGCC 5 cut(s) 129, 751, 1478, 1489, 1629
BtgI CCRYGG 2 cut(s) 27, 1630
BtrI CACGTC 1 cut(s) 2266
BtsCI GGATG 7 cut(s) 174, 211, 304, 937, 1663, 1774, 1864
BtsI GCAGTG 1 cut(s) 1051
BtsIMutI CAGTG 5 cut(s) 556, 1051, 1414, 1478, 1782
Cac8I GCNNGC 5 cut(s) 395, 630, 732, 896, 1428
CaiI CAGNNNCTG 2 cut(s) 1393, 2039
CciI TCATGA 1 cut(s) 1207
CfoI GCGC 1 cut(s) 405
Cfr13I GGNCC 3 cut(s) 1476, 1627, 1982
CseI GACGC 2 cut(s) 21, 1399
Csp6I GTAC 4 cut(s) 370, 1085, 1114, 1133
CviAII CATG 9 cut(s) 246, 629, 731, 854, 895, 952, 1208, 1502, 1631
CviQI GTAC 4 cut(s) 370, 1085, 1114, 1133
DdeI CTNAG 7 cut(s) 879, 1056, 1894, 2007, 2118, 2196, 2232
DpnI GATC 5 cut(s) 63, 859, 1303, 1506, 1788
DpnII GATC 5 cut(s) 61, 857, 1301, 1504, 1786
DraIII CACNNNGTG 1 cut(s) 653
DrdI GACNNNNNNGTC 1 cut(s) 1658
DseDI GACNNNNNNGTC 1 cut(s) 1658
EaeI YGGCCR 3 cut(s) 127, 749, 1487
Eam1104I CTCTTC 2 cut(s) 72, 613
EarI CTCTTC 2 cut(s) 72, 613
EciI GGCGGA 3 cut(s) 663, 1433, 1844
Eco130I CCWWGG 4 cut(s) 803, 816, 907, 1630
Eco24I GRGCYC 1 cut(s) 160
Eco32I GATATC 1 cut(s) 274
Eco47I GGWCC 1 cut(s) 1982
Eco57I CTGAAG 4 cut(s) 642, 941, 1704, 2016
Eco88I CYCGRG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 1982
EcoRII CCWGG 6 cut(s) 159, 676, 1581, 1719, 1924, 2296
EcoRV GATATC 1 cut(s) 274
EcoT14I CCWWGG 4 cut(s) 803, 816, 907, 1630
EcoT22I ATGCAT 1 cut(s) 788
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 4 cut(s) 803, 816, 907, 1630
FaeI CATG 9 cut(s) 249, 632, 734, 857, 898, 955, 1211, 1505, 1634
FalI AAGNNNNNCTT 4 cut(s) 69, 101, 1570, 1602
FaqI GGGAC 2 cut(s) 1943, 1968
FatI CATG 9 cut(s) 245, 628, 730, 853, 894, 951, 1207, 1501, 1630
FauI CCCGC 2 cut(s) 10, 1847
FauNDI CATATG 3 cut(s) 528, 831, 967
FbaI TGATCA 1 cut(s) 1786
FblI GTMKAC 1 cut(s) 1662
FokI GGATG 7 cut(s) 161, 218, 311, 944, 1670, 1781, 1871
FriOI GRGCYC 1 cut(s) 160
FspBI CTAG 7 cut(s) 461, 581, 804, 908, 1364, 2060, 2329
FspI TGCGCA 1 cut(s) 404
GlaI GCGC 1 cut(s) 404
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 3 cut(s) 682, 920, 2280
HaeIII GGCC 5 cut(s) 129, 751, 1478, 1489, 1629
HapII CCGG 1 cut(s) 1919
HgaI GACGC 2 cut(s) 21, 1399
HhaI GCGC 1 cut(s) 405
Hin1I GRCGYC 2 cut(s) 32, 841
Hin1II CATG 9 cut(s) 249, 632, 734, 857, 898, 955, 1211, 1505, 1634
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HincII GTYRAC 5 cut(s) 466, 769, 960, 1900, 2238
HindII GTYRAC 5 cut(s) 466, 769, 960, 1900, 2238
HindIII AAGCTT 1 cut(s) 706
HpaI GTTAAC 2 cut(s) 769, 960
HpaII CCGG 1 cut(s) 1919
HphI GGTGA 5 cut(s) 923, 1130, 1809, 1892, 2081
Hpy99I CGWCG 2 cut(s) 943, 2267
HpyAV CCTTC 2 cut(s) 1054, 2315
HpyCH4III ACNGT 7 cut(s) 666, 865, 886, 1039, 1285, 1409, 2173
HpyCH4IV ACGT 7 cut(s) 71, 505, 841, 1339, 1447, 1464, 2265
HpyF10VI GCNNNNNNNGC 5 cut(s) 638, 1565, 2096, 2197, 2258
HpyF3I CTNAG 7 cut(s) 879, 1056, 1894, 2007, 2118, 2196, 2232
HpySE526I ACGT 7 cut(s) 71, 505, 841, 1339, 1447, 1464, 2265
Hsp92I GRCGYC 2 cut(s) 32, 841
Hsp92II CATG 9 cut(s) 249, 632, 734, 857, 898, 955, 1211, 1505, 1634
HspAI GCGC 1 cut(s) 403
KflI GGGWCCC 1 cut(s) 1982
Kpn2I TCCGGA 1 cut(s) 1918
Ksp22I TGATCA 1 cut(s) 1786
KspAI GTTAAC 2 cut(s) 769, 960
Kzo9I GATC 5 cut(s) 61, 857, 1301, 1504, 1786
LguI GCTCTTC 2 cut(s) 72, 613
LmnI GCTCC 4 cut(s) 202, 676, 1079, 2299
LweI GCATC 6 cut(s) 196, 538, 795, 2044, 2083, 2239
MaeI CTAG 7 cut(s) 461, 581, 804, 908, 1364, 2060, 2329
MaeII ACGT 7 cut(s) 71, 505, 841, 1339, 1447, 1464, 2265
MaeIII GTNAC 4 cut(s) 865, 975, 1214, 2146
MalI GATC 5 cut(s) 63, 859, 1303, 1506, 1788
MboI GATC 5 cut(s) 61, 857, 1301, 1504, 1786
MflI RGATCY 2 cut(s) 857, 1301
MhlI GDGCHC 1 cut(s) 160
MlyI GAGTC 5 cut(s) 844, 1262, 1841, 2030, 2243
MmeI TCCRAC 5 cut(s) 54, 909, 1204, 1371, 1773
Mph1103I ATGCAT 1 cut(s) 788
MroI TCCGGA 1 cut(s) 1918
MroXI GAANNNNTTC 3 cut(s) 102, 1068, 1200
MseI TTAA 6 cut(s) 123, 768, 959, 1004, 1334, 1577
MslI CAYNNNNRTG 5 cut(s) 633, 834, 1157, 1212, 2008
MspA1I CMGCKG 2 cut(s) 641, 2258
MspCI CTTAAG 1 cut(s) 1576
MspI CCGG 1 cut(s) 1919
MspR9I CCNGG 6 cut(s) 161, 678, 1583, 1721, 1926, 2298
Mva1269I GAATGC 1 cut(s) 116
MvaI CCWGG 6 cut(s) 161, 678, 1583, 1721, 1926, 2298
MwoI GCNNNNNNNGC 5 cut(s) 638, 1565, 2096, 2197, 2258
NcoI CCATGG 1 cut(s) 1630
NdeI CATATG 3 cut(s) 528, 831, 967
NdeII GATC 5 cut(s) 61, 857, 1301, 1504, 1786
NlaIII CATG 9 cut(s) 249, 632, 734, 857, 898, 955, 1211, 1505, 1634
NlaIV GGNNCC 7 cut(s) 336, 859, 1923, 1983, 1984, 2046, 2295
NmuCI GTSAC 1 cut(s) 1214
NsbI TGCGCA 1 cut(s) 404
NsiI ATGCAT 1 cut(s) 788
NspI RCATGY 5 cut(s) 249, 632, 734, 898, 955
OliI CACNNNNGTG 1 cut(s) 2008
PaeI GCATGC 3 cut(s) 632, 734, 898
PagI TCATGA 1 cut(s) 1207
PciSI GCTCTTC 2 cut(s) 72, 613
PctI GAATGC 1 cut(s) 116
PdmI GAANNNNTTC 3 cut(s) 102, 1068, 1200
PfeI GAWTC 8 cut(s) 614, 743, 1069, 1384, 1676, 1943, 2165, 2207
PfoI TCCNGGA 1 cut(s) 2296
PleI GAGTC 5 cut(s) 844, 1262, 1840, 2030, 2242
PpsI GAGTC 5 cut(s) 844, 1262, 1840, 2030, 2242
Ppu21I YACGTR 1 cut(s) 1340
PpuMI RGGWCCY 1 cut(s) 1982
Psp5II RGGWCCY 1 cut(s) 1982
Psp6I CCWGG 6 cut(s) 159, 676, 1581, 1719, 1924, 2296
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 6 cut(s) 159, 676, 1581, 1719, 1924, 2296
PspN4I GGNNCC 7 cut(s) 336, 859, 1923, 1983, 1984, 2046, 2295
PspPI GGNCC 3 cut(s) 1476, 1627, 1982
PspPPI RGGWCCY 1 cut(s) 1982
PstI CTGCAG 3 cut(s) 426, 1401, 2257
PstNI CAGNNNCTG 2 cut(s) 1393, 2039
PsuI RGATCY 2 cut(s) 857, 1301
PvuII CAGCTG 1 cut(s) 2258
RsaI GTAC 4 cut(s) 371, 1086, 1115, 1134
RsaNI GTAC 4 cut(s) 370, 1085, 1114, 1133
RseI CAYNNNNRTG 5 cut(s) 633, 834, 1157, 1212, 2008
SapI GCTCTTC 2 cut(s) 72, 613
SaqAI TTAA 6 cut(s) 123, 768, 959, 1004, 1334, 1577
Sau3AI GATC 5 cut(s) 61, 857, 1301, 1504, 1786
Sau96I GGNCC 3 cut(s) 1476, 1627, 1982
SchI GAGTC 5 cut(s) 844, 1262, 1841, 2030, 2243
ScrFI CCNGG 6 cut(s) 161, 678, 1583, 1721, 1926, 2298
SduI GDGCHC 1 cut(s) 160
SfaNI GCATC 6 cut(s) 196, 538, 795, 2044, 2083, 2239
SfcI CTRYAG 3 cut(s) 422, 1397, 2253
SinI GGWCC 1 cut(s) 1982
SmiMI CAYNNNNRTG 5 cut(s) 633, 834, 1157, 1212, 2008
SmlI CTYRAG 2 cut(s) 1576, 2157
SmoI CTYRAG 2 cut(s) 1576, 2157
SphI GCATGC 3 cut(s) 632, 734, 898
SspMI CTAG 7 cut(s) 461, 581, 804, 908, 1364, 2060, 2329
StyD4I CCNGG 6 cut(s) 159, 676, 1581, 1719, 1924, 2296
StyI CCWWGG 4 cut(s) 803, 816, 907, 1630
TaaI ACNGT 7 cut(s) 666, 865, 886, 1039, 1285, 1409, 2173
TaiI ACGT 7 cut(s) 74, 508, 844, 1342, 1450, 1467, 2268
TaqI TCGA 3 cut(s) 1194, 1458, 1507
TatI WGTACW 2 cut(s) 1084, 1132
TauI GCSGC 5 cut(s) 132, 985, 1489, 2194, 2294
TfiI GAWTC 8 cut(s) 614, 743, 1069, 1384, 1676, 1943, 2165, 2207
Tru1I TTAA 6 cut(s) 123, 768, 959, 1004, 1334, 1577
Tru9I TTAA 6 cut(s) 123, 768, 959, 1004, 1334, 1577
TscAI CASTG 5 cut(s) 563, 1051, 1414, 1478, 1789
TseFI GTSAC 1 cut(s) 1214
Tsp45I GTSAC 1 cut(s) 1214
TspRI CASTG 5 cut(s) 563, 1051, 1414, 1478, 1789
Vha464I CTTAAG 1 cut(s) 1576
VpaK11BI GGWCC 1 cut(s) 1982
XapI RAATTY 5 cut(s) 230, 1092, 1249, 1599, 1744
XceI RCATGY 5 cut(s) 249, 632, 734, 898, 955
XmaJI CCTAGG 2 cut(s) 803, 907
XmiI GTMKAC 1 cut(s) 1662
XmnI GAANNNNTTC 3 cut(s) 102, 1068, 1200
XspI CTAG 7 cut(s) 461, 581, 804, 908, 1364, 2060, 2329
ZraI GACGTC 1 cut(s) 842
Zsp2I ATGCAT 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.