MD05G1274100.v1.1

Serine threonine-protein phosphatase 6 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002321
Physical Location & Seq
Forward (+)
40877018 .. 40886042
9025 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1274100.v1.1.491

Sequence Viewer

Length: 2334 bp
ATGTTCTGGCGTATGGCGGGGTTGTCCACGGCGTCTCCGGTGGAGACAATTTTGGACAAGGATAATTTCACGTTGGAAGAGCTACTTGATGAAGATGAAATTATTCAAGAATGCAAAGCTCTTAATGGCCGCCTAATCAATTTTTTGCGAGAAAGAGCCCAGGTTGAGCAACTCATCCGATACATAGTTGAAGAAGCTCCTGAGGATGCTGAAAAGAGACGAACTTTCAAATTTCCTTTTATTGCATGTGAGATTTTTACTTGTGAAGTTGATATCATACTCAAAACATTGGTAGAGGATGAGGAGTTGATGAACTTGTTGTTCTCCTTTTTGGAACCAAATAATTCTCACAGCACACTCTTGGCTGGGTACTTTAGCAAGGTTGTTGTTTGTCTGCTGTTGCGCAAGACAGTTCCTTTCCTGCAGTATATTCAGGGTCATCAAGATATTGTTAAGAAGCTAGTTGACCTGATTGGAATTACATCTATCATGGAGGTTTTGATACGTCTGGTTGGTGCTGACGAACACATGTATTCTAATCATACGGAGGCAATGCACTGGATACAAGATACAAATGTGCTCGAGATGATTGTGGACAAGTTCAGCTCTTCAGATTCTCCTGAAGTGCATGCTAATGCAGCGGAGACACTTTGTGCTATAACACGGTTTGCTCCGCCTGGACTTTCTTCTAAAATCTCCAGCCCAAGTTTTATAGGAAGATTGTTTTGGCATGCTTTGGAGGACTCACGGCCAAAATCTGTTCTTGTTAACTCGTTGTCAGTATGCATCTCTTTGCTAGACCCTAGGAGGCAAACTTTGGGAACATATCATATGTATGGCCGTCAGATGACTCATGGATCCACAGTTACTGCTAATCCTGAGACTGTTGTGGGCATGCTGGAGAGCCTAGGTGATTTGCTGAAGCTGTTGGATGTTTCATCGGTGGATAACACGCTGTTAACTACATATGGCAAGTTGCAGCCGCCTCTTGGAAAACATCGGTTAAAGGTTGTAGAGTTCATTTCAGTATTACTTACGGTTGGTAGTGAAGCTGCTGAGAAGGAATTGATTCGTCTTGGTGCTGTACAGAGAATTTTAGACTTGTTTTTTAAGTACCCATATAACAACTTTGTACATCACCACATAGAGAACATAATTGTGTCGTGTTTGGAAAGCAAGAATATTCCTCTTGTTGAACATCTTCTTCGTGAGTGTGACCTTGTTGGAAAAATACTTCAAGCAGAAAAGAATTTTACATTGGCAGCTGACGCGGACAAGGTGCCAACTGTTCCTGCTGAGGGTAGATCCCCACCCAGAATAGGGAACATTGGACATTTAACACGCATATCCAACAAACTCATTCAGCTTGGAAATAACAATAGCGAGATTCAGACGCTGCTGCAGGAAAACATTGAGTGGGCGGAGTGGCAGGCAAGTGTCCTGTCAAAGCGTAATACAGTCGAGAACGTCTATCAATGGGCCTGTGGGCGGCCAACTGCATTACATGATCGAAATAGGGATAGTGATGATGATGACTATCAAGATAGAGATTACGATGTTGCAGCCCTTGCAAATAACCTAAGCCAGGCTTTCCGATATGGAATATACAACAATGACGAGTTAGATGAGGGCCATGGCTCACTTGAACGGGATGATGAGGATGTCTACTTTGATGATGAATCTGCTGAAGTTGTTATATCTTCACTGCGTTTGGGAGATGACCAGGAAAGTGGTTCTCTGTTTACAAATTCCAACTGGTTTGCGTTTGAGGATGACAGGGCTGCCACTGATCGCTCAACTGGTTCTCTTGCTTCTCCATCACCAAATACGGTGGAGTCTAGTGCTATCAGTGGTGGCAGTGATGATGATGTCATTGTTGGTGAAGATGATGACTTGGTTGACACCGCAACATCTTCTCCAGAACCAGGAACAAAACCAGAAGATTCAGAAGTTGCCGTCCCCAACAATTCGCCAGAAGTGGGACCCGTTGAAACTGAAAAACCACCAGAGTGGGTTGAATGGAGGGAGAGTTCAGACTCTGGGGAACCATCTGATGTCCTACCAAATGGTGAGCATCAAACGGAATCTGCAGATACAGCTAAATGTTCTCCGTCCACTTCTGAGCCGCTGATAAAAGATGATAAAGTTGTTACTGAGCCTCAAGCTGAATCAACAGTTGAGGGAACTTCAAAGCCGCCTGAGCTGTCAGAATCAGGCAACGAGAAGCCAAGTTCTGAGTCGACCACTTCTGATGCTGCAGCTGCCGACGTGGGAACAAAAGGCAGTCAAGAAGCGGCTGCAAGTGATAAAGAGAAAGACAAGGAAGGGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAPS

Protein Analysis

778

Amino Acids

86.01

Weight (kDa)

4.52

Isoelectric Point (pI)

44.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAPS PF04499 131 - 490 2.8e-64 SIT4 phosphatase-associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 1279
AccI GTMKAC 2 cut(s) 1665, 2240
AccII CGCG 1 cut(s) 1271
AclWI GGATC 3 cut(s) 852, 865, 1299
AcoI YGGCCR 4 cut(s) 127, 749, 838, 1490
AcsI RAATTY 4 cut(s) 230, 1092, 1249, 1747
AcuI CTGAAG 4 cut(s) 594, 642, 941, 1707
AcyI GRCGYC 1 cut(s) 32
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 4 cut(s) 371, 1086, 1115, 1134
AfiI CCNNNNNNNGG 8 cut(s) 989, 1298, 1319, 1320, 1488, 1585, 1925, 1979
AflIII ACRYGT 1 cut(s) 528
AgsI TTSAA 9 cut(s) 107, 191, 229, 1196, 1238, 1646, 1991, 2018, 2190
AjiI CACGTC 1 cut(s) 2269
AjnI CCWGG 5 cut(s) 159, 676, 1584, 1722, 1924
AjuI GAANNNNNNNTTGG 4 cut(s) 709, 741, 1241, 1273
AleI CACNNNNGTG 1 cut(s) 2008
AloI GAACNNNNNNTCC 2 cut(s) 2014, 2046
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 5 cut(s) 38, 39, 211, 638, 875
AlwI GGATC 3 cut(s) 852, 865, 1299
AlwNI CAGNNNCTG 3 cut(s) 869, 1396, 2039
Ama87I CYCGRG 1 cut(s) 581
AoxI GGCC 6 cut(s) 127, 749, 838, 1479, 1490, 1630
ApoI RAATTY 4 cut(s) 230, 1092, 1249, 1747
Asp700I GAANNNNTTC 3 cut(s) 102, 1068, 1200
AspA2I CCTAGG 2 cut(s) 803, 907
AspLEI GCGC 1 cut(s) 405
AspS9I GGNCC 3 cut(s) 1479, 1630, 1982
AsuHPI GGTGA 5 cut(s) 923, 1130, 1812, 1892, 2081
AvaI CYCGRG 1 cut(s) 581
AvaII GGWCC 1 cut(s) 1982
AvrII CCTAGG 2 cut(s) 803, 907
AxyI CCTNAGG 1 cut(s) 201
BaeI ACNNNNGTAYC 2 cut(s) 561, 594
BamHI GGATCC 1 cut(s) 857
BanI GGYRCC 1 cut(s) 1279
BanII GRGCYC 1 cut(s) 160
Bbv12I GWGCWC 1 cut(s) 582
BbvCI CCTCAGC 1 cut(s) 1296
BccI CCATC 2 cut(s) 1825, 2056
BceAI ACGGC 4 cut(s) 45, 764, 825, 1940
BciT130I CCWGG 5 cut(s) 161, 678, 1586, 1724, 1926
BciVI GTATCC 1 cut(s) 555
BcoDI GTCTC 5 cut(s) 38, 39, 211, 638, 875
BfaI CTAG 5 cut(s) 461, 797, 804, 908, 1839
BfmI CTRYAG 4 cut(s) 422, 1400, 2088, 2256
BfuI GTATCC 1 cut(s) 555
BlnI CCTAGG 2 cut(s) 803, 907
Bme1390I CCNGG 5 cut(s) 161, 678, 1586, 1724, 1926
Bme18I GGWCC 1 cut(s) 1982
BmeT110I CYCGRG 1 cut(s) 581
BmgBI CACGTC 1 cut(s) 2269
BmgT120I GGNCC 3 cut(s) 1479, 1630, 1982
BmiI GGNNCC 6 cut(s) 336, 859, 1281, 1983, 1984, 2046
BmrFI CCNGG 5 cut(s) 161, 678, 1586, 1724, 1926
BmsI GCATC 4 cut(s) 196, 795, 2083, 2242
BpmI CTGGAG 3 cut(s) 682, 920, 1902
Bpu10I CCTNAGC 3 cut(s) 1296, 1580, 2199
BpuEI CTTGAG 1 cut(s) 2145
BsaBI GATNNNNATC 1 cut(s) 1536
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 5 cut(s) 27, 159, 803, 907, 1633
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 6 cut(s) 19, 49, 1900, 1930, 2014, 2044
Bsc4I CCNNNNNNNGG 8 cut(s) 989, 1298, 1319, 1320, 1488, 1585, 1925, 1979
Bse1I ACTGG 3 cut(s) 563, 1760, 1804
Bse21I CCTNAGG 1 cut(s) 201
Bse3DI GCAATG 1 cut(s) 558
Bse8I GATNNNNATC 1 cut(s) 1536
BseBI CCWGG 5 cut(s) 161, 678, 1586, 1724, 1926
BseDI CCNNGG 5 cut(s) 27, 159, 803, 907, 1633
BseGI GGATG 7 cut(s) 174, 211, 304, 937, 1657, 1666, 1777
BseJI GATNNNNATC 1 cut(s) 1536
BseLI CCNNNNNNNGG 8 cut(s) 989, 1298, 1319, 1320, 1488, 1585, 1925, 1979
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 8 cut(s) 192, 870, 1047, 1287, 2112, 2145, 2190, 2226
BseNI ACTGG 3 cut(s) 563, 1760, 1804
BseRI GAGGAG 1 cut(s) 317
BseYI CCCAGC 1 cut(s) 365
Bsh1236I CGCG 1 cut(s) 1271
BshFI GGCC 6 cut(s) 129, 751, 840, 1481, 1492, 1632
BshNI GGYRCC 1 cut(s) 1279
BsiHKAI GWGCWC 1 cut(s) 582
BsiHKCI CYCGRG 1 cut(s) 581
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 2 cut(s) 1943, 1995
BslI CCNNNNNNNGG 8 cut(s) 989, 1298, 1319, 1320, 1488, 1585, 1925, 1979
BsmAI GTCTC 5 cut(s) 38, 39, 211, 638, 875
BsmBI CGTCTC 2 cut(s) 39, 211
BsmFI GGGAC 2 cut(s) 1943, 1995
BsmI GAATGC 1 cut(s) 116
BsnI GGCC 6 cut(s) 129, 751, 840, 1481, 1492, 1632
BsoBI CYCGRG 1 cut(s) 581
Bsp1286I GDGCHC 2 cut(s) 160, 582
Bsp1407I TGTACA 2 cut(s) 1084, 1132
Bsp143I GATC 4 cut(s) 857, 1304, 1507, 1789
Bsp19I CCATGG 1 cut(s) 1633
BspANI GGCC 6 cut(s) 129, 751, 840, 1481, 1492, 1632
BspCNI CTCAG 8 cut(s) 193, 871, 1048, 1288, 2113, 2146, 2191, 2227
BspFNI CGCG 1 cut(s) 1271
BspLI GGNNCC 6 cut(s) 336, 859, 1281, 1983, 1984, 2046
BspMAI CTGCAG 4 cut(s) 426, 1404, 2092, 2260
BspPI GGATC 3 cut(s) 852, 865, 1299
BspQI GCTCTTC 2 cut(s) 72, 613
BspT107I GGYRCC 1 cut(s) 1279
BsrDI GCAATG 1 cut(s) 558
BsrGI TGTACA 2 cut(s) 1084, 1132
BsrI ACTGG 3 cut(s) 563, 1760, 1804
BssECI CCNNGG 5 cut(s) 27, 159, 803, 907, 1633
BssMI GATC 4 cut(s) 857, 1304, 1507, 1789
BssNI GRCGYC 1 cut(s) 32
BssT1I CCWWGG 3 cut(s) 803, 907, 1633
Bst2UI CCWGG 5 cut(s) 161, 678, 1586, 1724, 1926
Bst4CI ACNGT 9 cut(s) 412, 666, 865, 886, 1039, 1288, 1459, 1831, 2176
Bst6I CTCTTC 2 cut(s) 72, 613
BstACI GRCGYC 1 cut(s) 32
BstAPI GCANNNNNTGC 1 cut(s) 1568
BstAUI TGTACA 2 cut(s) 1084, 1132
BstC8I GCNNGC 4 cut(s) 630, 732, 896, 1431
BstDEI CTNAG 9 cut(s) 201, 879, 1056, 1296, 1580, 2121, 2154, 2199, 2235
BstDSI CCRYGG 2 cut(s) 27, 1633
BstENI CCTNNNNNAGG 1 cut(s) 1583
BstF5I GGATG 7 cut(s) 174, 211, 304, 937, 1657, 1666, 1777
BstFNI CGCG 1 cut(s) 1271
BstHHI GCGC 1 cut(s) 405
BstKTI GATC 4 cut(s) 860, 1307, 1510, 1792
BstMAI GTCTC 5 cut(s) 38, 39, 211, 638, 875
BstMBI GATC 4 cut(s) 857, 1304, 1507, 1789
BstMWI GCNNNNNNNGC 6 cut(s) 638, 1268, 1568, 2096, 2200, 2261
BstNI CCWGG 5 cut(s) 161, 678, 1586, 1724, 1926
BstNSI RCATGY 5 cut(s) 249, 532, 632, 734, 898
BstSCI CCNGG 5 cut(s) 159, 676, 1584, 1722, 1924
BstSFI CTRYAG 4 cut(s) 422, 1400, 2088, 2256
BstUI CGCG 1 cut(s) 1271
BstX2I RGATCY 2 cut(s) 857, 1304
BstXI CCANNNNNNTGG 2 cut(s) 1730, 2010
BstYI RGATCY 2 cut(s) 857, 1304
Bsu36I CCTNAGG 1 cut(s) 201
BsuI GTATCC 1 cut(s) 555
BsuRI GGCC 6 cut(s) 129, 751, 840, 1481, 1492, 1632
BtgI CCRYGG 2 cut(s) 27, 1633
BtrI CACGTC 1 cut(s) 2269
BtsCI GGATG 7 cut(s) 174, 211, 304, 937, 1657, 1666, 1777
BtsI GCAGTG 2 cut(s) 1703, 1864
BtsIMutI CAGTG 5 cut(s) 556, 1703, 1785, 1855, 1864
Cac8I GCNNGC 4 cut(s) 630, 732, 896, 1431
CaiI CAGNNNCTG 3 cut(s) 869, 1396, 2039
CfoI GCGC 1 cut(s) 405
Cfr13I GGNCC 3 cut(s) 1479, 1630, 1982
CseI GACGC 3 cut(s) 21, 1277, 1402
Csp6I GTAC 4 cut(s) 370, 1085, 1114, 1133
CspCI CAANNNNNGTGG 2 cut(s) 1812, 1847
CviAII CATG 9 cut(s) 246, 490, 529, 629, 731, 854, 895, 1505, 1634
CviQI GTAC 4 cut(s) 370, 1085, 1114, 1133
DdeI CTNAG 9 cut(s) 201, 879, 1056, 1296, 1580, 2121, 2154, 2199, 2235
DpnI GATC 4 cut(s) 859, 1306, 1509, 1791
DpnII GATC 4 cut(s) 857, 1304, 1507, 1789
DraIII CACNNNGTG 1 cut(s) 653
EaeI YGGCCR 4 cut(s) 127, 749, 838, 1490
Eam1104I CTCTTC 2 cut(s) 72, 613
EarI CTCTTC 2 cut(s) 72, 613
EciI GGCGGA 2 cut(s) 663, 1436
Eco130I CCWWGG 3 cut(s) 803, 907, 1633
Eco24I GRGCYC 1 cut(s) 160
Eco32I GATATC 1 cut(s) 274
Eco47I GGWCC 1 cut(s) 1982
Eco57I CTGAAG 4 cut(s) 594, 642, 941, 1707
Eco81I CCTNAGG 1 cut(s) 201
Eco88I CYCGRG 1 cut(s) 581
EcoNI CCTNNNNNAGG 1 cut(s) 1583
EcoO109I RGGNCCY 1 cut(s) 1982
EcoRII CCWGG 5 cut(s) 159, 676, 1584, 1722, 1924
EcoRV GATATC 1 cut(s) 274
EcoT14I CCWWGG 3 cut(s) 803, 907, 1633
EcoT22I ATGCAT 1 cut(s) 788
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 3 cut(s) 803, 907, 1633
Esp3I CGTCTC 2 cut(s) 39, 211
FaeI CATG 9 cut(s) 249, 493, 532, 632, 734, 857, 898, 1508, 1637
FalI AAGNNNNNCTT 4 cut(s) 69, 101, 1573, 1605
FaqI GGGAC 2 cut(s) 1943, 1995
FatI CATG 9 cut(s) 245, 489, 528, 628, 730, 853, 894, 1504, 1633
FauI CCCGC 1 cut(s) 10
FauNDI CATATG 2 cut(s) 831, 967
FblI GTMKAC 2 cut(s) 1665, 2240
FokI GGATG 7 cut(s) 161, 218, 311, 944, 1664, 1673, 1784
FriOI GRGCYC 1 cut(s) 160
FspBI CTAG 5 cut(s) 461, 797, 804, 908, 1839
FspI TGCGCA 1 cut(s) 404
GlaI GCGC 1 cut(s) 404
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 3 cut(s) 682, 920, 1902
HaeIII GGCC 6 cut(s) 129, 751, 840, 1481, 1492, 1632
HapII CCGG 1 cut(s) 38
HgaI GACGC 3 cut(s) 21, 1277, 1402
HhaI GCGC 1 cut(s) 405
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 9 cut(s) 249, 493, 532, 632, 734, 857, 898, 1508, 1637
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HincII GTYRAC 5 cut(s) 466, 769, 960, 1900, 2241
HindII GTYRAC 5 cut(s) 466, 769, 960, 1900, 2241
HpaI GTTAAC 2 cut(s) 769, 960
HpaII CCGG 1 cut(s) 38
HphI GGTGA 5 cut(s) 923, 1130, 1812, 1892, 2081
Hpy99I CGWCG 1 cut(s) 2270
HpyAV CCTTC 2 cut(s) 1054, 2318
HpyCH4III ACNGT 9 cut(s) 412, 666, 865, 886, 1039, 1288, 1459, 1831, 2176
HpyCH4IV ACGT 4 cut(s) 71, 505, 1467, 2268
HpyF10VI GCNNNNNNNGC 6 cut(s) 638, 1268, 1568, 2096, 2200, 2261
HpyF3I CTNAG 9 cut(s) 201, 879, 1056, 1296, 1580, 2121, 2154, 2199, 2235
HpySE526I ACGT 4 cut(s) 71, 505, 1467, 2268
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 9 cut(s) 249, 493, 532, 632, 734, 857, 898, 1508, 1637
HspAI GCGC 1 cut(s) 403
KflI GGGWCCC 1 cut(s) 1982
KspAI GTTAAC 2 cut(s) 769, 960
Kzo9I GATC 4 cut(s) 857, 1304, 1507, 1789
LguI GCTCTTC 2 cut(s) 72, 613
LmnI GCTCC 2 cut(s) 202, 676
LweI GCATC 4 cut(s) 196, 795, 2083, 2242
MaeI CTAG 5 cut(s) 461, 797, 804, 908, 1839
MaeII ACGT 4 cut(s) 71, 505, 1467, 2268
MaeIII GTNAC 3 cut(s) 865, 1214, 2149
MalI GATC 4 cut(s) 859, 1306, 1509, 1791
MboI GATC 4 cut(s) 857, 1304, 1507, 1789
MflI RGATCY 2 cut(s) 857, 1304
MhlI GDGCHC 2 cut(s) 160, 582
MlyI GAGTC 5 cut(s) 737, 844, 1844, 2030, 2246
MmeI TCCRAC 5 cut(s) 54, 909, 1204, 1374, 1776
Mph1103I ATGCAT 1 cut(s) 788
MroXI GAANNNNTTC 3 cut(s) 102, 1068, 1200
MseI TTAA 7 cut(s) 123, 453, 768, 959, 1004, 1110, 1337
MslI CAYNNNNRTG 4 cut(s) 633, 834, 1157, 2008
MspA1I CMGCKG 4 cut(s) 641, 1265, 2128, 2261
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 5 cut(s) 161, 678, 1586, 1724, 1926
Mva1269I GAATGC 1 cut(s) 116
MvaI CCWGG 5 cut(s) 161, 678, 1586, 1724, 1926
MvnI CGCG 1 cut(s) 1271
MwoI GCNNNNNNNGC 6 cut(s) 638, 1268, 1568, 2096, 2200, 2261
NcoI CCATGG 1 cut(s) 1633
NdeI CATATG 2 cut(s) 831, 967
NdeII GATC 4 cut(s) 857, 1304, 1507, 1789
NlaIII CATG 9 cut(s) 249, 493, 532, 632, 734, 857, 898, 1508, 1637
NlaIV GGNNCC 6 cut(s) 336, 859, 1281, 1983, 1984, 2046
NmuCI GTSAC 1 cut(s) 1214
NsbI TGCGCA 1 cut(s) 404
NsiI ATGCAT 1 cut(s) 788
NspI RCATGY 5 cut(s) 249, 532, 632, 734, 898
OliI CACNNNNGTG 1 cut(s) 2008
PaeI GCATGC 3 cut(s) 632, 734, 898
PaeR7I CTCGAG 1 cut(s) 581
PciI ACATGT 1 cut(s) 528
PciSI GCTCTTC 2 cut(s) 72, 613
PctI GAATGC 1 cut(s) 116
PdmI GAANNNNTTC 3 cut(s) 102, 1068, 1200
PfeI GAWTC 8 cut(s) 614, 1069, 1387, 1679, 1943, 2084, 2168, 2210
PleI GAGTC 5 cut(s) 737, 844, 1843, 2030, 2245
PpsI GAGTC 5 cut(s) 737, 844, 1843, 2030, 2245
PpuMI RGGWCCY 1 cut(s) 1982
PscI ACATGT 1 cut(s) 528
Psp5II RGGWCCY 1 cut(s) 1982
Psp6I CCWGG 5 cut(s) 159, 676, 1584, 1722, 1924
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 5 cut(s) 159, 676, 1584, 1722, 1924
PspN4I GGNNCC 6 cut(s) 336, 859, 1281, 1983, 1984, 2046
PspPI GGNCC 3 cut(s) 1479, 1630, 1982
PspPPI RGGWCCY 1 cut(s) 1982
PstI CTGCAG 4 cut(s) 426, 1404, 2092, 2260
PstNI CAGNNNCTG 3 cut(s) 869, 1396, 2039
PsuI RGATCY 2 cut(s) 857, 1304
PvuII CAGCTG 2 cut(s) 1265, 2261
RsaI GTAC 4 cut(s) 371, 1086, 1115, 1134
RsaNI GTAC 4 cut(s) 370, 1085, 1114, 1133
RseI CAYNNNNRTG 4 cut(s) 633, 834, 1157, 2008
SalI GTCGAC 1 cut(s) 2239
SapI GCTCTTC 2 cut(s) 72, 613
SaqAI TTAA 7 cut(s) 123, 453, 768, 959, 1004, 1110, 1337
Sau3AI GATC 4 cut(s) 857, 1304, 1507, 1789
Sau96I GGNCC 3 cut(s) 1479, 1630, 1982
SchI GAGTC 5 cut(s) 737, 844, 1844, 2030, 2246
ScrFI CCNGG 5 cut(s) 161, 678, 1586, 1724, 1926
SduI GDGCHC 2 cut(s) 160, 582
SfaNI GCATC 4 cut(s) 196, 795, 2083, 2242
SfcI CTRYAG 4 cut(s) 422, 1400, 2088, 2256
Sfr274I CTCGAG 1 cut(s) 581
SinI GGWCC 1 cut(s) 1982
SlaI CTCGAG 1 cut(s) 581
SmiMI CAYNNNNRTG 4 cut(s) 633, 834, 1157, 2008
SmlI CTYRAG 2 cut(s) 581, 2160
SmoI CTYRAG 2 cut(s) 581, 2160
SphI GCATGC 3 cut(s) 632, 734, 898
SspI AATATT 1 cut(s) 1183
SspMI CTAG 5 cut(s) 461, 797, 804, 908, 1839
StyD4I CCNGG 5 cut(s) 159, 676, 1584, 1722, 1924
StyI CCWWGG 3 cut(s) 803, 907, 1633
TaaI ACNGT 9 cut(s) 412, 666, 865, 886, 1039, 1288, 1459, 1831, 2176
TaiI ACGT 4 cut(s) 74, 508, 1470, 2271
TaqI TCGA 4 cut(s) 582, 1461, 1510, 2240
TatI WGTACW 2 cut(s) 1084, 1132
TauI GCSGC 6 cut(s) 132, 985, 1492, 2128, 2197, 2297
TfiI GAWTC 8 cut(s) 614, 1069, 1387, 1679, 1943, 2084, 2168, 2210
Tru1I TTAA 7 cut(s) 123, 453, 768, 959, 1004, 1110, 1337
Tru9I TTAA 7 cut(s) 123, 453, 768, 959, 1004, 1110, 1337
TscAI CASTG 5 cut(s) 563, 1710, 1792, 1855, 1864
TseFI GTSAC 1 cut(s) 1214
Tsp45I GTSAC 1 cut(s) 1214
TspDTI ATGAA 6 cut(s) 105, 111, 326, 927, 1009, 1692
TspGWI ACGGA 3 cut(s) 560, 2096, 2100
TspRI CASTG 5 cut(s) 563, 1710, 1792, 1855, 1864
VpaK11BI GGWCC 1 cut(s) 1982
XagI CCTNNNNNAGG 1 cut(s) 1583
XapI RAATTY 4 cut(s) 230, 1092, 1249, 1747
XceI RCATGY 5 cut(s) 249, 532, 632, 734, 898
XhoI CTCGAG 1 cut(s) 581
XmaJI CCTAGG 2 cut(s) 803, 907
XmiI GTMKAC 2 cut(s) 1665, 2240
XmnI GAANNNNTTC 3 cut(s) 102, 1068, 1200
XspI CTAG 5 cut(s) 461, 797, 804, 908, 1839
Zsp2I ATGCAT 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.