FvH4_3g16590

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006725
Physical Location & Seq
Forward (+)
10457763 .. 10461444
3682 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16590.t1

Sequence Viewer

Length: 1062 bp
ATGGGAGAGCGTAAGGTTCTGAACAAGTACTACCCGCCGGACTTCGACCCGTCGAAGCTCCCCCGAGTCCGGCGACCCAAGAATCAACAGATCAAGGTCCGTATGATGCTTCCGATGAGCATCCGATGCAACACCTGCGGCAACTACATCTACAAAGGCACCAAGTTCAACTCGAGGAAAGAAGACGTCGTCGGCGAGACGTATTTGGGGATTCAAATCTTTAGGTTTTACTTCAAGTGCACCAGGTGCTCGGCGGAGCTTGCAATGAAGACGGACCCGCAGAACTCGGATTATGTGGTGGAGGCCGGGGCGACTCGGAATTTCGAGCCGTGGCGTAATGAGGATGAGGAAGCGGATAAGGAGAAGCAGAAGAGGGAGTCGGAAGAAATGGGAGATGCTATGAAGTCTTTGGAGAATAGAACATTGGATTCGAAGAGAGAGATGGATATTCTTGCGGCTTTGGATGAGATGAAGTCCATGAAGTCCAGGCATGCCACTGTGAGTGTGGATGAGATGTTGACGGCTTTGCAGCATACGGTTGCGCACAAGGAGGAAAGGATAGAAGAGGAGGATGAAGCAATCTTAAAATCAATTGTATTCCATAACTCAAAAGATTATGTTCGAAGGATTCATGATGATGATTTAGATGATGATTTAGATGATGATGAAGACTTATTAGAGCCTTTTAGCAGCAATGGTGAAACGTCTAATCATCTGTCAAAGAGACAAAAGCTTACTCAAGATAATAGCAAACCAACAGATTCATTGACAAAAACCAGTATTTTTGATAGCTCAAGCAGCAGAGGAAAACAGAATGGTTCAGAAGATACTAAAATCATTTTCAAGTCTCCAACTGTTAGGCTGTCTGTTGTTAAGAAACCAGTGGTGGCTAAAGTTAACAATTTGGTGAAGGTAGAAGACCAGAAACAAGTTGGTGAGGAGTCTAAAAGTTTGACTGAACCAGATGAGAAAGAACAGGACGGCGAGAATAATATCAACAGTGGAAGCAACGTATTACAATCATTGTGCCAAAACTACAACAGTGATGATAGTGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

354

Amino Acids

40.49

Weight (kDa)

5.14

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 9 - 350 7.7e-102 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
AatII GACGTC 1 cut(s) 189
Acc16I TGCGCA 1 cut(s) 543
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 158
AciI CCGC 6 cut(s) 35, 138, 254, 278, 353, 455
AcsI RAATTY 1 cut(s) 319
AcyI GRCGYC 1 cut(s) 186
AdeI CACNNNGTG 1 cut(s) 246
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 4 cut(s) 169, 215, 235, 844
AjnI CCWGG 2 cut(s) 242, 485
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AluBI AGCT 4 cut(s) 58, 259, 733, 792
AluI AGCT 4 cut(s) 58, 259, 733, 792
Alw21I GWGCWC 2 cut(s) 242, 251
Alw26I GTCTC 3 cut(s) 191, 718, 852
Alw44I GTGCAC 1 cut(s) 238
Ama87I CYCGRG 2 cut(s) 63, 172
AoxI GGCC 1 cut(s) 303
ApaLI GTGCAC 1 cut(s) 238
ApeKI GCWGC 3 cut(s) 529, 690, 798
ApoI RAATTY 1 cut(s) 319
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 2 cut(s) 97, 274
AsuC2I CCSGG 1 cut(s) 307
AsuHPI GGTGA 3 cut(s) 710, 919, 947
AsuII TTCGAA 2 cut(s) 431, 622
AvaI CYCGRG 2 cut(s) 63, 172
AvaII GGWCC 2 cut(s) 97, 274
BaeGI GKGCMC 1 cut(s) 242
BanI GGYRCC 1 cut(s) 158
BbsI GAAGAC 4 cut(s) 189, 275, 675, 924
Bbv12I GWGCWC 2 cut(s) 242, 251
BbvI GCAGC 3 cut(s) 541, 702, 810
BccI CCATC 1 cut(s) 436
BceAI ACGGC 3 cut(s) 313, 537, 997
BciT130I CCWGG 2 cut(s) 244, 487
BcnI CCSGG 1 cut(s) 307
BcoDI GTCTC 3 cut(s) 191, 718, 852
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 5 cut(s) 139, 456, 530, 691, 799
BlsI GCNGC 5 cut(s) 140, 457, 531, 692, 800
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 3 cut(s) 244, 307, 487
Bme18I GGWCC 2 cut(s) 97, 274
BmeT110I CYCGRG 2 cut(s) 63, 172
BmgT120I GGNCC 2 cut(s) 97, 274
BmiI GGNNCC 2 cut(s) 160, 276
BmrFI CCNGG 3 cut(s) 244, 307, 487
BmsI GCATC 4 cut(s) 96, 116, 129, 385
BpiI GAAGAC 4 cut(s) 189, 275, 675, 924
Bpu14I TTCGAA 2 cut(s) 431, 622
BpuEI CTTGAG 2 cut(s) 723, 778
BpuMI CCSGG 1 cut(s) 307
BsaBI GATNNNNATC 2 cut(s) 119, 215
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 2 cut(s) 306, 329
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse1I ACTGG 2 cut(s) 777, 881
Bse3DI GCAATG 2 cut(s) 270, 700
Bse8I GATNNNNATC 2 cut(s) 119, 215
BseBI CCWGG 2 cut(s) 244, 487
BseDI CCNNGG 2 cut(s) 306, 329
BseGI GGATG 5 cut(s) 120, 349, 469, 514, 577
BseJI GATNNNNATC 2 cut(s) 119, 215
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMI GCAATG 2 cut(s) 270, 700
BseNI ACTGG 2 cut(s) 777, 881
BseRI GAGGAG 2 cut(s) 581, 953
BseSI GKGCMC 1 cut(s) 242
BseXI GCAGC 3 cut(s) 541, 702, 810
BshFI GGCC 1 cut(s) 305
BshNI GGYRCC 1 cut(s) 158
BsiHKAI GWGCWC 2 cut(s) 242, 251
BsiHKCI CYCGRG 2 cut(s) 63, 172
BsiSI CCGG 3 cut(s) 38, 70, 306
BslI CCNNNNNNNGG 1 cut(s) 69
BsmAI GTCTC 3 cut(s) 191, 718, 852
BsmBI CGTCTC 1 cut(s) 191
BsnI GGCC 1 cut(s) 305
BsoBI CYCGRG 2 cut(s) 63, 172
Bsp119I TTCGAA 2 cut(s) 431, 622
Bsp1286I GDGCHC 2 cut(s) 242, 251
Bsp143I GATC 1 cut(s) 90
BspACI CCGC 6 cut(s) 35, 138, 254, 278, 353, 455
BspANI GGCC 1 cut(s) 305
BspHI TCATGA 1 cut(s) 631
BspLI GGNNCC 2 cut(s) 160, 276
BspMI ACCTGC 1 cut(s) 143
BspT104I TTCGAA 2 cut(s) 431, 622
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 2 cut(s) 270, 700
BsrI ACTGG 2 cut(s) 777, 881
BssECI CCNNGG 2 cut(s) 306, 329
BssMI GATC 1 cut(s) 90
BssNI GRCGYC 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 244, 487
Bst4CI ACNGT 5 cut(s) 499, 538, 856, 1001, 1043
Bst6I CTCTTC 3 cut(s) 365, 428, 558
BstACI GRCGYC 1 cut(s) 186
BstAPI GCANNNNNTGC 3 cut(s) 126, 135, 246
BstBI TTCGAA 2 cut(s) 431, 622
BstC8I GCNNGC 2 cut(s) 261, 492
BstDSI CCRYGG 1 cut(s) 329
BstF5I GGATG 5 cut(s) 120, 349, 469, 514, 577
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 1 cut(s) 93
BstMAI GTCTC 3 cut(s) 191, 718, 852
BstMBI GATC 1 cut(s) 90
BstMWI GCNNNNNNNGC 5 cut(s) 126, 135, 246, 260, 798
BstNI CCWGG 2 cut(s) 244, 487
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 3 cut(s) 242, 305, 485
BstSLI GKGCMC 1 cut(s) 242
BstV1I GCAGC 3 cut(s) 541, 702, 810
BstV2I GAAGAC 4 cut(s) 189, 275, 675, 924
BsuRI GGCC 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 329
BtsCI GGATG 5 cut(s) 120, 349, 469, 514, 577
BtsIMutI CAGTG 4 cut(s) 495, 888, 1006, 1048
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 261, 492
CciI TCATGA 1 cut(s) 631
CfoI GCGC 1 cut(s) 544
Cfr13I GGNCC 2 cut(s) 97, 274
CsiI ACCWGGT 1 cut(s) 242
Csp6I GTAC 1 cut(s) 28
CviAII CATG 3 cut(s) 478, 491, 632
CviQI GTAC 1 cut(s) 28
DpnI GATC 1 cut(s) 92
DpnII GATC 1 cut(s) 90
DraIII CACNNNGTG 1 cut(s) 246
Eam1104I CTCTTC 3 cut(s) 365, 428, 558
EarI CTCTTC 3 cut(s) 365, 428, 558
EciI GGCGGA 1 cut(s) 269
Eco47I GGWCC 2 cut(s) 97, 274
Eco88I CYCGRG 2 cut(s) 63, 172
EcoRII CCWGG 2 cut(s) 242, 485
Esp3I CGTCTC 1 cut(s) 191
FaeI CATG 3 cut(s) 481, 494, 635
FaiI YATR 9 cut(s) 104, 294, 401, 479, 492, 534, 603, 618, 633
FatI CATG 3 cut(s) 477, 490, 631
FauI CCCGC 2 cut(s) 42, 285
Fnu4HI GCNGC 5 cut(s) 139, 456, 530, 691, 799
FokI GGATG 5 cut(s) 107, 356, 476, 521, 584
Fsp4HI GCNGC 5 cut(s) 139, 456, 530, 691, 799
FspI TGCGCA 1 cut(s) 543
GlaI GCGC 1 cut(s) 543
GluI GCNGC 5 cut(s) 139, 456, 530, 691, 799
HaeIII GGCC 1 cut(s) 305
HapII CCGG 3 cut(s) 38, 70, 306
HhaI GCGC 1 cut(s) 544
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 3 cut(s) 481, 494, 635
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 2 cut(s) 519, 898
HindII GTYRAC 2 cut(s) 519, 898
HindIII AAGCTT 1 cut(s) 731
HinfI GANTC 9 cut(s) 66, 82, 211, 313, 377, 428, 628, 761, 941
HpaI GTTAAC 1 cut(s) 898
HpaII CCGG 3 cut(s) 38, 70, 306
HphI GGTGA 3 cut(s) 710, 919, 947
Hpy166II GTNNAC 3 cut(s) 240, 519, 898
Hpy188I TCNGA 7 cut(s) 21, 114, 125, 289, 318, 382, 823
Hpy188III TCNNGA 2 cut(s) 632, 740
Hpy8I GTNNAC 3 cut(s) 240, 519, 898
Hpy99I CGWCG 3 cut(s) 55, 191, 194
HpyAV CCTTC 2 cut(s) 618, 904
HpyCH4III ACNGT 5 cut(s) 499, 538, 856, 1001, 1043
HpyCH4IV ACGT 4 cut(s) 186, 200, 704, 1011
HpyCH4V TGCA 4 cut(s) 129, 240, 263, 529
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 135, 246, 260, 798
HpySE526I ACGT 4 cut(s) 186, 200, 704, 1011
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 3 cut(s) 481, 494, 635
HspAI GCGC 1 cut(s) 542
KspAI GTTAAC 1 cut(s) 898
Kzo9I GATC 1 cut(s) 90
LmnI GCTCC 2 cut(s) 63, 256
Lsp1109I GCAGC 3 cut(s) 541, 702, 810
LweI GCATC 4 cut(s) 96, 116, 129, 385
MabI ACCWGGT 1 cut(s) 242
MaeII ACGT 4 cut(s) 186, 200, 704, 1011
MalI GATC 1 cut(s) 92
MboI GATC 1 cut(s) 90
MboII GAAGA 9 cut(s) 194, 280, 382, 395, 445, 575, 680, 836, 929
MfeI CAATTG 1 cut(s) 591
MhlI GDGCHC 2 cut(s) 242, 251
MluCI AATT 3 cut(s) 319, 591, 901
MlyI GAGTC 4 cut(s) 75, 307, 386, 950
MmeI TCCRAC 2 cut(s) 360, 875
MseI TTAA 3 cut(s) 584, 873, 897
MslI CAYNNNNRTG 1 cut(s) 636
MspI CCGG 3 cut(s) 38, 70, 306
MspR9I CCNGG 3 cut(s) 244, 307, 487
MunI CAATTG 1 cut(s) 591
MvaI CCWGG 2 cut(s) 244, 487
MwoI GCNNNNNNNGC 5 cut(s) 126, 135, 246, 260, 798
NciI CCSGG 1 cut(s) 307
NdeII GATC 1 cut(s) 90
NlaIII CATG 3 cut(s) 481, 494, 635
NlaIV GGNNCC 2 cut(s) 160, 276
NmeAIII GCCGAG 1 cut(s) 230
NsbI TGCGCA 1 cut(s) 543
NspI RCATGY 1 cut(s) 494
NspV TTCGAA 2 cut(s) 431, 622
PaeI GCATGC 1 cut(s) 494
PaeR7I CTCGAG 1 cut(s) 172
PagI TCATGA 1 cut(s) 631
PaqCI CACCTGC 1 cut(s) 143
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PfeI GAWTC 5 cut(s) 82, 211, 428, 628, 761
PflFI GACNNNGTC 1 cut(s) 188
PkrI GCNGC 5 cut(s) 140, 457, 531, 692, 800
PleI GAGTC 4 cut(s) 74, 307, 385, 949
PpsI GAGTC 4 cut(s) 74, 307, 385, 949
Psp6I CCWGG 2 cut(s) 242, 485
PspGI CCWGG 2 cut(s) 242, 485
PspN4I GGNNCC 2 cut(s) 160, 276
PspPI GGNCC 2 cut(s) 97, 274
PspXI VCTCGAGB 1 cut(s) 172
PsrI GAACNNNNNNTAC 2 cut(s) 14, 46
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
RseI CAYNNNNRTG 1 cut(s) 636
SaqAI TTAA 3 cut(s) 584, 873, 897
SatI GCNGC 5 cut(s) 139, 456, 530, 691, 799
Sau3AI GATC 1 cut(s) 90
Sau96I GGNCC 2 cut(s) 97, 274
ScaI AGTACT 1 cut(s) 29
SchI GAGTC 4 cut(s) 75, 307, 386, 950
ScrFI CCNGG 3 cut(s) 244, 307, 487
SduI GDGCHC 2 cut(s) 242, 251
SexAI ACCWGGT 1 cut(s) 242
SfaNI GCATC 4 cut(s) 96, 116, 129, 385
Sfr274I CTCGAG 1 cut(s) 172
SfuI TTCGAA 2 cut(s) 431, 622
SinI GGWCC 2 cut(s) 97, 274
SlaI CTCGAG 1 cut(s) 172
SmiMI CAYNNNNRTG 1 cut(s) 636
SmlI CTYRAG 3 cut(s) 172, 738, 793
SmoI CTYRAG 3 cut(s) 172, 738, 793
SphI GCATGC 1 cut(s) 494
Sse9I AATT 3 cut(s) 319, 591, 901
SsiI CCGC 6 cut(s) 35, 138, 254, 278, 353, 455
StyD4I CCNGG 3 cut(s) 242, 305, 485
TaaI ACNGT 5 cut(s) 499, 538, 856, 1001, 1043
TaiI ACGT 4 cut(s) 189, 203, 707, 1014
TaqI TCGA 6 cut(s) 45, 53, 173, 324, 431, 622
TasI AATT 3 cut(s) 319, 591, 901
TatI WGTACW 1 cut(s) 27
TauI GCSGC 2 cut(s) 141, 458
TfiI GAWTC 5 cut(s) 82, 211, 428, 628, 761
Tru1I TTAA 3 cut(s) 584, 873, 897
Tru9I TTAA 3 cut(s) 584, 873, 897
TscAI CASTG 4 cut(s) 502, 888, 1006, 1048
TseI GCWGC 3 cut(s) 529, 690, 798
TspDTI ATGAA 8 cut(s) 281, 416, 485, 494, 588, 620, 681, 753
TspGWI ACGGA 2 cut(s) 89, 287
TspRI CASTG 4 cut(s) 502, 888, 1006, 1048
Tth111I GACNNNGTC 1 cut(s) 188
VneI GTGCAC 1 cut(s) 238
VpaK11BI GGWCC 2 cut(s) 97, 274
XapI RAATTY 1 cut(s) 319
XceI RCATGY 1 cut(s) 494
XcmI CCANNNNNNNNNTGG 2 cut(s) 502, 929
XhoI CTCGAG 1 cut(s) 172
ZraI GACGTC 1 cut(s) 187
ZrmI AGTACT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.