MD10G1195300.v1.1

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006725
Physical Location & Seq
Reverse (-)
29386373 .. 29390194
3822 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1195300.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGGGAGAACGTAAGGTTCTGAACAAGTACTATCCGCCGGACTTCGACCCTTCGAAGCTGCCCCGGATCAGGAGGCCGAAGAATCAACAGATAAAGGTGCGGATGATGCTTCCGATGAGCATTCGGTGCAACACCTGCGGCAATTACATCTACAAAGGCACCAAGTTCAATTCTCGCAAAGAAGATGTCGTTGGAGAGACGTATTTGGGAATTCAAATATTCAGATTTTACTTCAAATGTACCAAGTGCTCTGCGGAACTCGCGATGAAGACAGACCCTCAGAATTCTGACTATGTTGTTGAGGCCGGTGCGACGCGTAATTTTGAACCCTGGCGCAATGAAGATGAGGAAGTAGATAAGGAGAAACAGAAAAGGGAATCCGAAGAAATGGGAGATGCCATGAGAGCCTTGGAGAACAGAACACAGGATTCAAAGAGAGAGATGGACGTCATGGCTGCTTTGGATGAGATGAAGTCCATGAAGTCCAGGCATGCGACTGTGAGTGTGGATGCTATGTTGGAGGCTTTGAAACAAACTTCTGCCCAAAAGGAAAAAAGGATTGAAGACGAGGATGAAGCAGTCATAAAATCAATAGTATTCCATAACTCAAAAGATTATATTAAAAGGATTCATGATGAAGATTTAGATGATGAAGAAGATATGATTGAGCCTTTAAGTGGCAACAGCGAAACATCAACCCATAAATTGAAGCGGCAAAAGCTTACTCAAGGGCTTCCTAGCAATCCGACAGATTGGTTGACAAAAAGCAGTATTTCTGATACCTCAAGCAACGGAGGGAATCGAAATACTTTCAAGTCTTCGAATGTTAGGGTTGCTGTAGTTAAGAAACCAGTTGTGGCTGAAGGTAAAAAGCCAGCGGAACCAGAAGACAAGAAACAATTGGGAGAGGAGGTTCAAAATTCGAAAAAACCAAATGAGAAGAAACAAGAGGGAGAGAATAAAAGCAGTGCTACAGCCAATGTATTGCAATCATTGTGCCAAAACTATGATAGCGACGACAGTGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

344

Amino Acids

39.23

Weight (kDa)

6.03

Isoelectric Point (pI)

46.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 9 - 340 4.4e-103 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
AatII GACGTC 1 cut(s) 450
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 158
AccII CGCG 2 cut(s) 263, 316
AciI CCGC 6 cut(s) 35, 100, 138, 254, 712, 878
AclWI GGATC 1 cut(s) 74
AcsI RAATTY 3 cut(s) 210, 283, 919
AcuI CTGAAG 1 cut(s) 882
AcyI GRCGYC 1 cut(s) 447
AfaI GTAC 2 cut(s) 29, 241
AfiI CCNNNNNNNGG 2 cut(s) 69, 677
AflIII ACRYGT 1 cut(s) 314
AjnI CCWGG 2 cut(s) 329, 485
AjuI GAANNNNNNNTTGG 2 cut(s) 174, 206
AloI GAACNNNNNNTCC 2 cut(s) 897, 929
AluBI AGCT 2 cut(s) 58, 721
AluI AGCT 2 cut(s) 58, 721
Alw21I GWGCWC 1 cut(s) 251
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 1 cut(s) 74
AoxI GGCC 2 cut(s) 74, 303
ApeKI GCWGC 2 cut(s) 58, 455
ApoI RAATTY 3 cut(s) 210, 283, 919
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AspLEI GCGC 1 cut(s) 336
AsuC2I CCSGG 1 cut(s) 64
AsuII TTCGAA 3 cut(s) 53, 821, 923
BanI GGYRCC 1 cut(s) 158
BbsI GAAGAC 4 cut(s) 275, 570, 810, 894
Bbv12I GWGCWC 1 cut(s) 251
BbvI GCAGC 2 cut(s) 45, 442
BccI CCATC 1 cut(s) 436
BciT130I CCWGG 2 cut(s) 331, 487
BcnI CCSGG 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 1 cut(s) 738
BfmI CTRYAG 2 cut(s) 837, 972
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 4 cut(s) 59, 139, 456, 713
BlsI GCNGC 4 cut(s) 60, 140, 457, 714
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 3 cut(s) 64, 331, 487
BmiI GGNNCC 2 cut(s) 160, 882
BmrFI CCNGG 3 cut(s) 64, 331, 487
BmsI GCATC 3 cut(s) 96, 385, 499
BpiI GAAGAC 4 cut(s) 275, 570, 810, 894
Bpu14I TTCGAA 3 cut(s) 53, 821, 923
BpuEI CTTGAG 2 cut(s) 711, 769
BpuMI CCSGG 1 cut(s) 64
BsaHI GRCGYC 1 cut(s) 447
BsaJI CCNNGG 3 cut(s) 62, 329, 408
BsaXI ACNNNNNCTCC 2 cut(s) 897, 927
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 677
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 1 cut(s) 851
Bse3DI GCAATG 1 cut(s) 343
BseBI CCWGG 2 cut(s) 331, 487
BseDI CCNNGG 3 cut(s) 62, 329, 408
BseGI GGATG 4 cut(s) 108, 469, 514, 577
BseLI CCNNNNNNNGG 2 cut(s) 69, 677
BseMI GCAATG 1 cut(s) 343
BseMII CTCAG 1 cut(s) 293
BseNI ACTGG 1 cut(s) 851
BseRI GAGGAG 1 cut(s) 923
BseXI GCAGC 2 cut(s) 45, 442
Bsh1236I CGCG 2 cut(s) 263, 316
BshFI GGCC 2 cut(s) 76, 305
BshNI GGYRCC 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 251
BsiSI CCGG 3 cut(s) 38, 64, 306
BslI CCNNNNNNNGG 2 cut(s) 69, 677
BsmAI GTCTC 1 cut(s) 191
BsmBI CGTCTC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 120
BsnI GGCC 2 cut(s) 76, 305
Bsp119I TTCGAA 3 cut(s) 53, 821, 923
Bsp1286I GDGCHC 1 cut(s) 251
Bsp143I GATC 1 cut(s) 66
Bsp68I TCGCGA 1 cut(s) 263
BspACI CCGC 6 cut(s) 35, 100, 138, 254, 712, 878
BspANI GGCC 2 cut(s) 76, 305
BspCNI CTCAG 1 cut(s) 292
BspFNI CGCG 2 cut(s) 263, 316
BspHI TCATGA 1 cut(s) 631
BspLI GGNNCC 2 cut(s) 160, 882
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 1 cut(s) 74
BspT104I TTCGAA 3 cut(s) 53, 821, 923
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 1 cut(s) 851
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 3 cut(s) 62, 329, 408
BssMI GATC 1 cut(s) 66
BssNI GRCGYC 1 cut(s) 447
BssT1I CCWWGG 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 331, 487
Bst4CI ACNGT 2 cut(s) 499, 1022
BstACI GRCGYC 1 cut(s) 447
BstAPI GCANNNNNTGC 2 cut(s) 126, 135
BstBI TTCGAA 3 cut(s) 53, 821, 923
BstC8I GCNNGC 2 cut(s) 492, 876
BstDEI CTNAG 1 cut(s) 279
BstF5I GGATG 4 cut(s) 108, 469, 514, 577
BstFNI CGCG 2 cut(s) 263, 316
BstHHI GCGC 1 cut(s) 336
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 6 cut(s) 106, 126, 135, 260, 404, 718
BstNI CCWGG 2 cut(s) 331, 487
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 3 cut(s) 62, 329, 485
BstSFI CTRYAG 2 cut(s) 837, 972
BstUI CGCG 2 cut(s) 263, 316
BstV1I GCAGC 2 cut(s) 45, 442
BstV2I GAAGAC 4 cut(s) 275, 570, 810, 894
BsuRI GGCC 2 cut(s) 76, 305
BtgZI GCGATG 1 cut(s) 278
BtsCI GGATG 4 cut(s) 108, 469, 514, 577
BtsI GCAGTG 1 cut(s) 973
BtsIMutI CAGTG 2 cut(s) 973, 1027
BtuMI TCGCGA 1 cut(s) 263
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 492, 876
CciI TCATGA 1 cut(s) 631
CfoI GCGC 1 cut(s) 336
Cfr10I RCCGGY 1 cut(s) 305
CseI GACGC 1 cut(s) 322
Csp6I GTAC 2 cut(s) 28, 240
CviAII CATG 5 cut(s) 400, 451, 478, 491, 632
CviQI GTAC 2 cut(s) 28, 240
DdeI CTNAG 1 cut(s) 279
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
EciI GGCGGA 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 408
Eco57I CTGAAG 1 cut(s) 882
EcoRI GAATTC 2 cut(s) 210, 283
EcoRII CCWGG 2 cut(s) 329, 485
EcoT14I CCWWGG 1 cut(s) 408
ErhI CCWWGG 1 cut(s) 408
Esp3I CGTCTC 1 cut(s) 191
FaeI CATG 5 cut(s) 403, 454, 481, 494, 635
FatI CATG 5 cut(s) 399, 450, 477, 490, 631
Fnu4HI GCNGC 4 cut(s) 59, 139, 456, 713
FokI GGATG 4 cut(s) 115, 476, 521, 584
Fsp4HI GCNGC 4 cut(s) 59, 139, 456, 713
FspBI CTAG 1 cut(s) 738
GlaI GCGC 1 cut(s) 335
GluI GCNGC 4 cut(s) 59, 139, 456, 713
HaeIII GGCC 2 cut(s) 76, 305
HapII CCGG 3 cut(s) 38, 64, 306
HgaI GACGC 1 cut(s) 322
HhaI GCGC 1 cut(s) 336
Hin1I GRCGYC 1 cut(s) 447
Hin1II CATG 5 cut(s) 403, 454, 481, 494, 635
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HincII GTYRAC 1 cut(s) 759
HindII GTYRAC 1 cut(s) 759
HindIII AAGCTT 1 cut(s) 719
HinfI GANTC 5 cut(s) 82, 377, 428, 628, 799
HpaII CCGG 3 cut(s) 38, 64, 306
Hpy166II GTNNAC 1 cut(s) 759
Hpy188I TCNGA 8 cut(s) 21, 114, 224, 282, 289, 382, 747, 778
Hpy188III TCNNGA 3 cut(s) 70, 262, 632
Hpy8I GTNNAC 1 cut(s) 759
Hpy99I CGWCG 2 cut(s) 316, 1019
HpyAV CCTTC 2 cut(s) 60, 857
HpyCH4III ACNGT 2 cut(s) 499, 1022
HpyCH4IV ACGT 3 cut(s) 10, 200, 447
HpyCH4V TGCA 2 cut(s) 129, 988
HpyF10VI GCNNNNNNNGC 6 cut(s) 106, 126, 135, 260, 404, 718
HpyF3I CTNAG 1 cut(s) 279
HpySE526I ACGT 3 cut(s) 10, 200, 447
Hsp92I GRCGYC 1 cut(s) 447
Hsp92II CATG 5 cut(s) 403, 454, 481, 494, 635
HspAI GCGC 1 cut(s) 334
Kzo9I GATC 1 cut(s) 66
Lsp1109I GCAGC 2 cut(s) 45, 442
LweI GCATC 3 cut(s) 96, 385, 499
MaeI CTAG 1 cut(s) 738
MaeII ACGT 3 cut(s) 10, 200, 447
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MfeI CAATTG 1 cut(s) 899
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 8 cut(s) 142, 169, 210, 283, 319, 704, 899, 919
MluI ACGCGT 1 cut(s) 314
MmeI TCCRAC 3 cut(s) 172, 498, 770
MseI TTAA 3 cut(s) 621, 674, 843
MspA1I CMGCKG 1 cut(s) 878
MspI CCGG 3 cut(s) 38, 64, 306
MspR9I CCNGG 3 cut(s) 64, 331, 487
MunI CAATTG 1 cut(s) 899
Mva1269I GAATGC 1 cut(s) 120
MvaI CCWGG 2 cut(s) 331, 487
MvnI CGCG 2 cut(s) 263, 316
MwoI GCNNNNNNNGC 6 cut(s) 106, 126, 135, 260, 404, 718
NciI CCSGG 1 cut(s) 64
NdeII GATC 1 cut(s) 66
NlaIII CATG 5 cut(s) 403, 454, 481, 494, 635
NlaIV GGNNCC 2 cut(s) 160, 882
NruI TCGCGA 1 cut(s) 263
NspI RCATGY 1 cut(s) 494
NspV TTCGAA 3 cut(s) 53, 821, 923
PaeI GCATGC 1 cut(s) 494
PagI TCATGA 1 cut(s) 631
PaqCI CACCTGC 1 cut(s) 143
PctI GAATGC 1 cut(s) 120
PfeI GAWTC 5 cut(s) 82, 377, 428, 628, 799
PkrI GCNGC 4 cut(s) 60, 140, 457, 714
Psp6I CCWGG 2 cut(s) 329, 485
PspGI CCWGG 2 cut(s) 329, 485
PspN4I GGNNCC 2 cut(s) 160, 882
RruI TCGCGA 1 cut(s) 263
RsaI GTAC 2 cut(s) 29, 241
RsaNI GTAC 2 cut(s) 28, 240
SaqAI TTAA 3 cut(s) 621, 674, 843
SatI GCNGC 4 cut(s) 59, 139, 456, 713
Sau3AI GATC 1 cut(s) 66
ScaI AGTACT 1 cut(s) 29
ScrFI CCNGG 3 cut(s) 64, 331, 487
SduI GDGCHC 1 cut(s) 251
SfaNI GCATC 3 cut(s) 96, 385, 499
SfcI CTRYAG 2 cut(s) 837, 972
SfuI TTCGAA 3 cut(s) 53, 821, 923
SmlI CTYRAG 2 cut(s) 726, 784
SmoI CTYRAG 2 cut(s) 726, 784
SphI GCATGC 1 cut(s) 494
Sse9I AATT 8 cut(s) 142, 169, 210, 283, 319, 704, 899, 919
SsiI CCGC 6 cut(s) 35, 100, 138, 254, 712, 878
SspI AATATT 1 cut(s) 219
SspMI CTAG 1 cut(s) 738
StyD4I CCNGG 3 cut(s) 62, 329, 485
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 2 cut(s) 499, 1022
TaiI ACGT 3 cut(s) 13, 203, 450
TaqI TCGA 5 cut(s) 45, 53, 802, 821, 923
TasI AATT 8 cut(s) 142, 169, 210, 283, 319, 704, 899, 919
TatI WGTACW 1 cut(s) 27
TauI GCSGC 2 cut(s) 141, 715
TfiI GAWTC 5 cut(s) 82, 377, 428, 628, 799
Tru1I TTAA 3 cut(s) 621, 674, 843
Tru9I TTAA 3 cut(s) 621, 674, 843
TscAI CASTG 2 cut(s) 973, 1027
TseI GCWGC 2 cut(s) 58, 455
TspDTI ATGAA 8 cut(s) 281, 354, 485, 494, 588, 620, 651, 666
TspGWI ACGGA 1 cut(s) 807
TspRI CASTG 2 cut(s) 973, 1027
XapI RAATTY 3 cut(s) 210, 283, 919
XceI RCATGY 1 cut(s) 494
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XspI CTAG 1 cut(s) 738
ZraI GACGTC 1 cut(s) 448
ZrmI AGTACT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.