Rroxscaffold_1G00051890

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006725
Physical Location & Seq
Reverse (-)
72652881 .. 72656663
3783 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00051890.1

Sequence Viewer

Length: 1050 bp
ATGGGAGAGCGTAAGGTTCTCAACAAGTACTACCCGCCGGACTTCGACCTGTCCAAGCTGCCCCGAGTCCGGCGGCCCAAGAACCAGCAGATTAAGGTCCGTATGATGCTTCCGATGAGCATCAGATGCAACACCTGCGGCAACTACATCTACAAGGGCACCAAATTCAACTCGCGGAAAGAAGATGTCGTCGGCGAGACGTACTTGGGAATTCAAATCTTTAGATTTTACTTCAAGTGCAAGTGCACCAAGTGCTCGGCGGAGCTTGCGATGAAGACGGACCCGCAGAACTCAGACTATGTGGTGGAGGCCGGGGCTACACGAAATTTCGAACCTTGGCGTAATGAGGATGAGGAAGTGGATAAGGAGAAGCAGAAGAGGGAGGCCGAAGAAATGGGAGATGCCATGAAATCTTTGGAGAACAGAACCTTGGATTCAAAAAGAGAAATGGATATCCTTGCCGCTTTGGATGAGATGAAGTCCATGAAGTCCAGGCATGCAACTGTGAGTGTGGATGAGATGTTGGAGGCTTTGCAACATACGGTTGCGCACAAGGAGAAAAGGCTGGAAGAGGAGGATGAAGCAGTCATAAAATCGATCGTATTCCATAACTCAAAAGATTATGTTCGAAGGATTGATGATGATGATTTGGATGATGATGAAGATTTATTACAGCCGGTAAGTGGCAATGGTGAAACGTCTAATCATCTGTCAAAGAAACAAAAGCTTACTCAAGATAATAGCAAACCAACAGATTTGTTGACAAAAACCAGTATTTCTGATAGCTCAAGCGGCAGAGGAAAACAGAATGGTTCAGAAGATTCTAAAATCATTTTCAAGTCTCCAACTGTTAGGCTTTCAGTGGTTAAGAAACCAGTGGTGGCTAAAGTTAACAATTTGGCGAAAGTGGATGAGAAAGAAGTTGGAGAGGAGTCCAAAAGTTTGACTAAAGATGAGAAAGAAGAGGACGACAAGAATACGACCAACGGTGGAAGCAACGTATTACAATCGCTGTTCCAAAACTACAACAGTGATGATAGTGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

349

Amino Acids

39.86

Weight (kDa)

5.48

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 9 - 346 4.5e-99 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
Acc16I TGCGCA 1 cut(s) 549
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 158
AccII CGCG 1 cut(s) 175
AciI CCGC 8 cut(s) 35, 73, 138, 175, 260, 284, 462, 792
AcsI RAATTY 3 cut(s) 164, 210, 325
AdeI CACNNNGTG 1 cut(s) 252
AfaI GTAC 2 cut(s) 29, 203
AfiI CCNNNNNNNGG 2 cut(s) 69, 683
AgsI TTSAA 5 cut(s) 169, 215, 235, 438, 838
AjnI CCWGG 1 cut(s) 491
AjuI GAANNNNNNNTTGG 2 cut(s) 413, 445
AluBI AGCT 4 cut(s) 58, 265, 727, 786
AluI AGCT 4 cut(s) 58, 265, 727, 786
Alw21I GWGCWC 2 cut(s) 248, 257
Alw26I GTCTC 2 cut(s) 191, 846
Alw44I GTGCAC 1 cut(s) 244
Ama87I CYCGRG 1 cut(s) 63
AoxI GGCC 3 cut(s) 74, 309, 384
ApaLI GTGCAC 1 cut(s) 244
ApeKI GCWGC 1 cut(s) 58
ApoI RAATTY 3 cut(s) 164, 210, 325
AspLEI GCGC 1 cut(s) 550
AspS9I GGNCC 3 cut(s) 75, 97, 280
AsuC2I CCSGG 1 cut(s) 313
AsuHPI GGTGA 1 cut(s) 704
AsuII TTCGAA 2 cut(s) 330, 628
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 2 cut(s) 97, 280
BaeGI GKGCMC 2 cut(s) 161, 248
BanI GGYRCC 1 cut(s) 158
BarI GAAGNNNNNNTAC 2 cut(s) 654, 686
BbsI GAAGAC 1 cut(s) 281
Bbv12I GWGCWC 2 cut(s) 248, 257
BbvI GCAGC 1 cut(s) 45
BciT130I CCWGG 1 cut(s) 493
BcnI CCSGG 1 cut(s) 313
BcoDI GTCTC 2 cut(s) 191, 846
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 5 cut(s) 59, 74, 139, 462, 793
BlsI GCNGC 5 cut(s) 60, 75, 140, 463, 794
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 2 cut(s) 313, 493
Bme18I GGWCC 2 cut(s) 97, 280
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 3 cut(s) 75, 97, 280
BmiI GGNNCC 2 cut(s) 160, 282
BmrFI CCNGG 2 cut(s) 313, 493
BmsI GCATC 4 cut(s) 96, 116, 129, 391
BpiI GAAGAC 1 cut(s) 281
Bpu14I TTCGAA 2 cut(s) 330, 628
BpuEI CTTGAG 2 cut(s) 717, 772
BpuMI CCSGG 1 cut(s) 313
Bsa29I ATCGAT 1 cut(s) 596
BsaBI GATNNNNATC 1 cut(s) 119
BsaJI CCNNGG 3 cut(s) 312, 335, 429
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 683
Bse118I RCCGGY 1 cut(s) 676
Bse1I ACTGG 2 cut(s) 771, 875
Bse3DI GCAATG 1 cut(s) 694
Bse8I GATNNNNATC 1 cut(s) 119
BseBI CCWGG 1 cut(s) 493
BseCI ATCGAT 1 cut(s) 596
BseDI CCNNGG 3 cut(s) 312, 335, 429
BseGI GGATG 6 cut(s) 355, 475, 520, 583, 658, 916
BseJI GATNNNNATC 1 cut(s) 119
BseLI CCNNNNNNNGG 2 cut(s) 69, 683
BseMI GCAATG 1 cut(s) 694
BseMII CTCAG 1 cut(s) 306
BseNI ACTGG 2 cut(s) 771, 875
BseRI GAGGAG 2 cut(s) 587, 944
BseSI GKGCMC 2 cut(s) 161, 248
BseXI GCAGC 1 cut(s) 45
Bsh1236I CGCG 1 cut(s) 175
Bsh1285I CGRYCG 1 cut(s) 600
BshFI GGCC 3 cut(s) 76, 311, 386
BshNI GGYRCC 1 cut(s) 158
BshVI ATCGAT 1 cut(s) 596
BsiEI CGRYCG 1 cut(s) 600
BsiHKAI GWGCWC 2 cut(s) 248, 257
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 4 cut(s) 38, 70, 312, 677
BslI CCNNNNNNNGG 2 cut(s) 69, 683
BsmAI GTCTC 2 cut(s) 191, 846
BsmBI CGTCTC 1 cut(s) 191
BsnI GGCC 3 cut(s) 76, 311, 386
BsoBI CYCGRG 1 cut(s) 63
Bsp119I TTCGAA 2 cut(s) 330, 628
Bsp1286I GDGCHC 3 cut(s) 161, 248, 257
Bsp143I GATC 1 cut(s) 597
BspACI CCGC 8 cut(s) 35, 73, 138, 175, 260, 284, 462, 792
BspANI GGCC 3 cut(s) 76, 311, 386
BspCNI CTCAG 1 cut(s) 305
BspDI ATCGAT 1 cut(s) 596
BspFNI CGCG 1 cut(s) 175
BspLI GGNNCC 2 cut(s) 160, 282
BspMI ACCTGC 1 cut(s) 143
BspT104I TTCGAA 2 cut(s) 330, 628
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 694
BsrFI RCCGGY 1 cut(s) 676
BsrI ACTGG 2 cut(s) 771, 875
BssAI RCCGGY 1 cut(s) 676
BssECI CCNNGG 3 cut(s) 312, 335, 429
BssMI GATC 1 cut(s) 597
BssT1I CCWWGG 2 cut(s) 335, 429
Bst2UI CCWGG 1 cut(s) 493
Bst4CI ACNGT 5 cut(s) 505, 544, 850, 989, 1031
Bst6I CTCTTC 3 cut(s) 371, 564, 957
BstAPI GCANNNNNTGC 3 cut(s) 126, 135, 252
BstBI TTCGAA 2 cut(s) 330, 628
BstC8I GCNNGC 2 cut(s) 267, 498
BstDEI CTNAG 1 cut(s) 292
BstF5I GGATG 6 cut(s) 355, 475, 520, 583, 658, 916
BstFNI CGCG 1 cut(s) 175
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 1 cut(s) 600
BstMAI GTCTC 2 cut(s) 191, 846
BstMBI GATC 1 cut(s) 597
BstMCI CGRYCG 1 cut(s) 600
BstMWI GCNNNNNNNGC 5 cut(s) 126, 135, 252, 266, 792
BstNI CCWGG 1 cut(s) 493
BstNSI RCATGY 1 cut(s) 500
BstSCI CCNGG 2 cut(s) 311, 491
BstSLI GKGCMC 2 cut(s) 161, 248
BstUI CGCG 1 cut(s) 175
BstV1I GCAGC 1 cut(s) 45
BstV2I GAAGAC 1 cut(s) 281
Bsu15I ATCGAT 1 cut(s) 596
BsuRI GGCC 3 cut(s) 76, 311, 386
BsuTUI ATCGAT 1 cut(s) 596
BtgZI GCGATG 1 cut(s) 284
BtsCI GGATG 6 cut(s) 355, 475, 520, 583, 658, 916
BtsIMutI CAGTG 3 cut(s) 867, 882, 1036
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 267, 498
CfoI GCGC 1 cut(s) 550
Cfr10I RCCGGY 1 cut(s) 676
Cfr13I GGNCC 3 cut(s) 75, 97, 280
ClaI ATCGAT 1 cut(s) 596
Csp6I GTAC 2 cut(s) 28, 202
CviAII CATG 3 cut(s) 406, 484, 497
CviQI GTAC 2 cut(s) 28, 202
DdeI CTNAG 1 cut(s) 292
DpnI GATC 1 cut(s) 599
DpnII GATC 1 cut(s) 597
DraIII CACNNNGTG 1 cut(s) 252
Eam1104I CTCTTC 3 cut(s) 371, 564, 957
EarI CTCTTC 3 cut(s) 371, 564, 957
EciI GGCGGA 1 cut(s) 275
Eco130I CCWWGG 2 cut(s) 335, 429
Eco32I GATATC 1 cut(s) 454
Eco47I GGWCC 2 cut(s) 97, 280
Eco88I CYCGRG 1 cut(s) 63
EcoRI GAATTC 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 491
EcoRV GATATC 1 cut(s) 454
EcoT14I CCWWGG 2 cut(s) 335, 429
ErhI CCWWGG 2 cut(s) 335, 429
Esp3I CGTCTC 1 cut(s) 191
FaeI CATG 3 cut(s) 409, 487, 500
FaiI YATR 9 cut(s) 104, 300, 407, 485, 498, 540, 590, 609, 624
FatI CATG 3 cut(s) 405, 483, 496
FauI CCCGC 2 cut(s) 42, 291
Fnu4HI GCNGC 5 cut(s) 59, 74, 139, 462, 793
FokI GGATG 6 cut(s) 362, 482, 527, 590, 665, 923
Fsp4HI GCNGC 5 cut(s) 59, 74, 139, 462, 793
FspI TGCGCA 1 cut(s) 549
GlaI GCGC 1 cut(s) 549
GluI GCNGC 5 cut(s) 59, 74, 139, 462, 793
HaeIII GGCC 3 cut(s) 76, 311, 386
HapII CCGG 4 cut(s) 38, 70, 312, 677
HhaI GCGC 1 cut(s) 550
Hin1II CATG 3 cut(s) 409, 487, 500
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HincII GTYRAC 2 cut(s) 762, 892
HindII GTYRAC 2 cut(s) 762, 892
HindIII AAGCTT 1 cut(s) 725
HinfI GANTC 4 cut(s) 66, 434, 821, 932
HpaI GTTAAC 1 cut(s) 892
HpaII CCGG 4 cut(s) 38, 70, 312, 677
HphI GGTGA 1 cut(s) 704
Hpy166II GTNNAC 3 cut(s) 246, 762, 892
Hpy188I TCNGA 5 cut(s) 114, 125, 295, 781, 817
Hpy188III TCNNGA 1 cut(s) 734
Hpy8I GTNNAC 3 cut(s) 246, 762, 892
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 1 cut(s) 624
HpyCH4III ACNGT 5 cut(s) 505, 544, 850, 989, 1031
HpyCH4IV ACGT 3 cut(s) 200, 698, 999
HpyCH4V TGCA 5 cut(s) 129, 240, 246, 500, 535
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 135, 252, 266, 792
HpyF3I CTNAG 1 cut(s) 292
HpySE526I ACGT 3 cut(s) 200, 698, 999
Hsp92II CATG 3 cut(s) 409, 487, 500
HspAI GCGC 1 cut(s) 548
KspAI GTTAAC 1 cut(s) 892
Kzo9I GATC 1 cut(s) 597
LmnI GCTCC 1 cut(s) 262
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 4 cut(s) 96, 116, 129, 391
MaeII ACGT 3 cut(s) 200, 698, 999
MalI GATC 1 cut(s) 599
MboI GATC 1 cut(s) 597
MboII GAAGA 8 cut(s) 194, 286, 388, 401, 581, 674, 830, 974
MhlI GDGCHC 3 cut(s) 161, 248, 257
MluCI AATT 4 cut(s) 164, 210, 325, 895
MlyI GAGTC 2 cut(s) 75, 941
MmeI TCCRAC 3 cut(s) 504, 869, 904
MseI TTAA 3 cut(s) 93, 867, 891
MspI CCGG 4 cut(s) 38, 70, 312, 677
MspR9I CCNGG 2 cut(s) 313, 493
MvaI CCWGG 1 cut(s) 493
MvnI CGCG 1 cut(s) 175
MwoI GCNNNNNNNGC 5 cut(s) 126, 135, 252, 266, 792
NciI CCSGG 1 cut(s) 313
NdeII GATC 1 cut(s) 597
NlaIII CATG 3 cut(s) 409, 487, 500
NlaIV GGNNCC 2 cut(s) 160, 282
NmeAIII GCCGAG 1 cut(s) 236
NsbI TGCGCA 1 cut(s) 549
NspI RCATGY 1 cut(s) 500
NspV TTCGAA 2 cut(s) 330, 628
PaeI GCATGC 1 cut(s) 500
PaqCI CACCTGC 1 cut(s) 143
PfeI GAWTC 2 cut(s) 434, 821
PkrI GCNGC 5 cut(s) 60, 75, 140, 463, 794
Ple19I CGATCG 1 cut(s) 600
PleI GAGTC 2 cut(s) 74, 940
PpsI GAGTC 2 cut(s) 74, 940
Psp6I CCWGG 1 cut(s) 491
PspGI CCWGG 1 cut(s) 491
PspN4I GGNNCC 2 cut(s) 160, 282
PspPI GGNCC 3 cut(s) 75, 97, 280
PvuI CGATCG 1 cut(s) 600
RsaI GTAC 2 cut(s) 29, 203
RsaNI GTAC 2 cut(s) 28, 202
SaqAI TTAA 3 cut(s) 93, 867, 891
SatI GCNGC 5 cut(s) 59, 74, 139, 462, 793
Sau3AI GATC 1 cut(s) 597
Sau96I GGNCC 3 cut(s) 75, 97, 280
ScaI AGTACT 1 cut(s) 29
SchI GAGTC 2 cut(s) 75, 941
ScrFI CCNGG 2 cut(s) 313, 493
SduI GDGCHC 3 cut(s) 161, 248, 257
SfaNI GCATC 4 cut(s) 96, 116, 129, 391
SfuI TTCGAA 2 cut(s) 330, 628
SinI GGWCC 2 cut(s) 97, 280
SmlI CTYRAG 2 cut(s) 732, 787
SmoI CTYRAG 2 cut(s) 732, 787
SphI GCATGC 1 cut(s) 500
Sse9I AATT 4 cut(s) 164, 210, 325, 895
SsiI CCGC 8 cut(s) 35, 73, 138, 175, 260, 284, 462, 792
StyD4I CCNGG 2 cut(s) 311, 491
StyI CCWWGG 2 cut(s) 335, 429
TaaI ACNGT 5 cut(s) 505, 544, 850, 989, 1031
TaiI ACGT 3 cut(s) 203, 701, 1002
TaqI TCGA 4 cut(s) 45, 330, 596, 628
TasI AATT 4 cut(s) 164, 210, 325, 895
TatI WGTACW 1 cut(s) 27
TauI GCSGC 4 cut(s) 76, 141, 464, 795
TfiI GAWTC 2 cut(s) 434, 821
Tru1I TTAA 3 cut(s) 93, 867, 891
Tru9I TTAA 3 cut(s) 93, 867, 891
TscAI CASTG 3 cut(s) 867, 882, 1036
TseI GCWGC 1 cut(s) 58
TspDTI ATGAA 6 cut(s) 287, 422, 491, 500, 594, 675
TspGWI ACGGA 2 cut(s) 89, 293
TspRI CASTG 3 cut(s) 867, 882, 1036
VneI GTGCAC 1 cut(s) 244
VpaK11BI GGWCC 2 cut(s) 97, 280
XapI RAATTY 3 cut(s) 164, 210, 325
XceI RCATGY 1 cut(s) 500
XcmI CCANNNNNNNNNTGG 1 cut(s) 412
ZrmI AGTACT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.