MD05G1208800.v1.1

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006725
Physical Location & Seq
Reverse (-)
34072849 .. 34076356
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1208800.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGGGAGAACGTAAGGTTCTGAACAAGTACTATCCGCCGGACTTCGACCCCTCGAAGCTGCCCAGGATCAGGAGGCCAAAGAATCAACAGATAAAGGTGCGGATGATGCTTCCGATGAGCATTCGCTGCAACACCTGCGGCAATTACATCTACAAAGGCACCAAATTTAATTCTCGCAAAGAAGATGTCGTTGGAGAGACGTATTTGGGAATTCAAATATTCAGATTTTACTTCAAATGTACCAAGTGCTCTGCCGAGCTCGCGATGAAGACAGACCCACAGAATTCTGACTATGTTGTTGAGGCTGGTGCGACACGTAATTTTGAACCCTGGCGCAATGAAGATGAGGAAGTAGATAAGGAGAAACAGAAAAGGGAGTCCGAAGAAATGGGAGATGCTATGAGAGCCTTGGAGAATAGAACACAGGATTCAAAAAGAGAGATGGACGTCATGGCTGCCTTGGATGAGATGAAGTCCATGAAGTCCAGGCATGCGACTGTGAGTGTGGATGCTATGTTGGAGGCTTTGAAACAAACATCCGTGCAAAAGGAAAAAAGGATTGAAGAGGAGGATGAAGCAGTCATAAAATCAATAGTATTCCATAACTCAAAAGACTATATTAAAAGAATTCACGATGAAGATTTAGATGATGAAGAAGATATGATTGAGCCTTTAAGTGGCAACGGTGAAACCTCACCGAAGCGGAAAAAGCTTACTCACGGGCTTCCTACCAATTCAACAGATTGGTTGACAAACAGCAGTATTTCTGATAGAGGGAATCGAAATGGCTCAGAAGCTGCTAAATTCCCTTTTAAGTCCTCCACTGTTAGGGTTGCTATAGTTAAGAAACCAGTTGCGGTTGAAGGCAAAAAGTCAGCGGAAGCAGAAGACAAGAAGAAACAATCGGGAGAGGAGGTTCAAAATTCGACAAAACCAGATAAGAGCAAAACCAATGCTACAACCAATGTTTTACAATCATTGTGCCAATATGACAGTGACGATAGTGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

338

Amino Acids

38.52

Weight (kDa)

6.24

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 9 - 335 6.3e-103 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
AatII GACGTC 1 cut(s) 450
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 158
AccII CGCG 1 cut(s) 263
AciI CCGC 6 cut(s) 35, 100, 138, 703, 857, 878
AclWI GGATC 1 cut(s) 74
AcsI RAATTY 6 cut(s) 164, 210, 283, 627, 803, 922
AcyI GRCGYC 1 cut(s) 447
AfaI GTAC 2 cut(s) 29, 241
AfiI CCNNNNNNNGG 3 cut(s) 69, 677, 828
AflIII ACRYGT 1 cut(s) 314
AgsI TTSAA 9 cut(s) 215, 235, 326, 432, 529, 563, 738, 863, 920
AjnI CCWGG 3 cut(s) 62, 329, 485
AjuI GAANNNNNNNTTGG 2 cut(s) 174, 206
AloI GAACNNNNNNTCC 2 cut(s) 900, 932
AluBI AGCT 4 cut(s) 58, 259, 712, 797
AluI AGCT 4 cut(s) 58, 259, 712, 797
Alw21I GWGCWC 2 cut(s) 251, 261
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 1 cut(s) 74
AlwNI CAGNNNCTG 1 cut(s) 797
AoxI GGCC 1 cut(s) 74
ApeKI GCWGC 4 cut(s) 58, 126, 455, 797
ApoI RAATTY 6 cut(s) 164, 210, 283, 627, 803, 922
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AspLEI GCGC 1 cut(s) 336
AsuHPI GGTGA 2 cut(s) 687, 698
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 261
BbsI GAAGAC 2 cut(s) 275, 894
Bbv12I GWGCWC 2 cut(s) 251, 261
BbvI GCAGC 4 cut(s) 45, 113, 442, 784
BccI CCATC 1 cut(s) 436
BciT130I CCWGG 3 cut(s) 64, 331, 487
BcoDI GTCTC 1 cut(s) 191
BfmI CTRYAG 1 cut(s) 837
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 5 cut(s) 59, 127, 139, 456, 798
BlsI GCNGC 5 cut(s) 60, 128, 140, 457, 799
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 3 cut(s) 64, 331, 487
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 3 cut(s) 64, 331, 487
BmsI GCATC 3 cut(s) 96, 385, 499
BpiI GAAGAC 2 cut(s) 275, 894
BsaAI YACGTR 1 cut(s) 317
BsaHI GRCGYC 1 cut(s) 447
BsaJI CCNNGG 4 cut(s) 62, 329, 408, 459
BsaXI ACNNNNNCTCC 2 cut(s) 900, 930
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 677, 828
Bse1I ACTGG 1 cut(s) 851
Bse3DI GCAATG 1 cut(s) 343
BseBI CCWGG 3 cut(s) 64, 331, 487
BseDI CCNNGG 4 cut(s) 62, 329, 408, 459
BseGI GGATG 5 cut(s) 108, 469, 514, 536, 577
BseLI CCNNNNNNNGG 3 cut(s) 69, 677, 828
BseMI GCAATG 1 cut(s) 343
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 1 cut(s) 851
BseRI GAGGAG 2 cut(s) 581, 926
BseXI GCAGC 4 cut(s) 45, 113, 442, 784
Bsh1236I CGCG 1 cut(s) 263
BshFI GGCC 1 cut(s) 76
BshNI GGYRCC 1 cut(s) 158
BsiHKAI GWGCWC 2 cut(s) 251, 261
BsiSI CCGG 1 cut(s) 38
BslI CCNNNNNNNGG 3 cut(s) 69, 677, 828
BsmAI GTCTC 1 cut(s) 191
BsmBI CGTCTC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 120
BsnI GGCC 1 cut(s) 76
Bsp1286I GDGCHC 2 cut(s) 251, 261
Bsp143I GATC 1 cut(s) 66
Bsp68I TCGCGA 1 cut(s) 263
BspACI CCGC 6 cut(s) 35, 100, 138, 703, 857, 878
BspANI GGCC 1 cut(s) 76
BspCNI CTCAG 1 cut(s) 803
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 1 cut(s) 160
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 1 cut(s) 74
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 343
BsrI ACTGG 1 cut(s) 851
BssECI CCNNGG 4 cut(s) 62, 329, 408, 459
BssMI GATC 1 cut(s) 66
BssNI GRCGYC 1 cut(s) 447
BssT1I CCWWGG 2 cut(s) 408, 459
Bst2UI CCWGG 3 cut(s) 64, 331, 487
Bst4CI ACNGT 4 cut(s) 499, 686, 826, 995
Bst6I CTCTTC 1 cut(s) 558
BstACI GRCGYC 1 cut(s) 447
BstAPI GCANNNNNTGC 2 cut(s) 126, 135
BstBAI YACGTR 1 cut(s) 317
BstC8I GCNNGC 2 cut(s) 261, 492
BstDEI CTNAG 1 cut(s) 790
BstF5I GGATG 5 cut(s) 108, 469, 514, 536, 577
BstFNI CGCG 1 cut(s) 263
BstHHI GCGC 1 cut(s) 336
BstKTI GATC 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 6 cut(s) 106, 126, 135, 260, 404, 709
BstNI CCWGG 3 cut(s) 64, 331, 487
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 3 cut(s) 62, 329, 485
BstSFI CTRYAG 1 cut(s) 837
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 4 cut(s) 45, 113, 442, 784
BstV2I GAAGAC 2 cut(s) 275, 894
BsuRI GGCC 1 cut(s) 76
BtgZI GCGATG 1 cut(s) 278
BtsCI GGATG 5 cut(s) 108, 469, 514, 536, 577
BtsIMutI CAGTG 2 cut(s) 822, 1000
BtuMI TCGCGA 1 cut(s) 263
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 261, 492
CaiI CAGNNNCTG 1 cut(s) 797
CfoI GCGC 1 cut(s) 336
Csp6I GTAC 2 cut(s) 28, 240
CviAII CATG 3 cut(s) 451, 478, 491
CviQI GTAC 2 cut(s) 28, 240
DdeI CTNAG 1 cut(s) 790
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
EciI GGCGGA 1 cut(s) 24
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 2 cut(s) 408, 459
Eco24I GRGCYC 1 cut(s) 261
Eco53kI GAGCTC 1 cut(s) 259
EcoICRI GAGCTC 1 cut(s) 259
EcoRI GAATTC 3 cut(s) 210, 283, 627
EcoRII CCWGG 3 cut(s) 62, 329, 485
EcoT14I CCWWGG 2 cut(s) 408, 459
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 2 cut(s) 408, 459
Esp3I CGTCTC 1 cut(s) 191
FaeI CATG 3 cut(s) 454, 481, 494
FatI CATG 3 cut(s) 450, 477, 490
Fnu4HI GCNGC 5 cut(s) 59, 127, 139, 456, 798
FokI GGATG 5 cut(s) 115, 476, 521, 523, 584
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 5 cut(s) 59, 127, 139, 456, 798
GlaI GCGC 1 cut(s) 335
GluI GCNGC 5 cut(s) 59, 127, 139, 456, 798
HaeIII GGCC 1 cut(s) 76
HapII CCGG 1 cut(s) 38
HhaI GCGC 1 cut(s) 336
Hin1I GRCGYC 1 cut(s) 447
Hin1II CATG 3 cut(s) 454, 481, 494
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HincII GTYRAC 1 cut(s) 750
HindII GTYRAC 1 cut(s) 750
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 4 cut(s) 82, 377, 428, 778
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 687, 698
Hpy166II GTNNAC 1 cut(s) 750
Hpy188I TCNGA 7 cut(s) 21, 114, 224, 289, 382, 769, 793
Hpy188III TCNNGA 4 cut(s) 70, 262, 632, 906
Hpy8I GTNNAC 1 cut(s) 750
HpyAV CCTTC 1 cut(s) 857
HpyCH4III ACNGT 4 cut(s) 499, 686, 826, 995
HpyCH4IV ACGT 4 cut(s) 10, 200, 316, 447
HpyCH4V TGCA 2 cut(s) 129, 544
HpyF10VI GCNNNNNNNGC 6 cut(s) 106, 126, 135, 260, 404, 709
HpyF3I CTNAG 1 cut(s) 790
HpySE526I ACGT 4 cut(s) 10, 200, 316, 447
Hsp92I GRCGYC 1 cut(s) 447
Hsp92II CATG 3 cut(s) 454, 481, 494
HspAI GCGC 1 cut(s) 334
Kzo9I GATC 1 cut(s) 66
Lsp1109I GCAGC 4 cut(s) 45, 113, 442, 784
LweI GCATC 3 cut(s) 96, 385, 499
MaeII ACGT 4 cut(s) 10, 200, 316, 447
MaeIII GTNAC 1 cut(s) 995
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MhlI GDGCHC 2 cut(s) 251, 261
MlyI GAGTC 1 cut(s) 386
MmeI TCCRAC 2 cut(s) 172, 498
MseI TTAA 5 cut(s) 168, 621, 674, 813, 843
MspA1I CMGCKG 1 cut(s) 878
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 3 cut(s) 64, 331, 487
Mva1269I GAATGC 1 cut(s) 120
MvaI CCWGG 3 cut(s) 64, 331, 487
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 6 cut(s) 106, 126, 135, 260, 404, 709
NdeII GATC 1 cut(s) 66
NlaIII CATG 3 cut(s) 454, 481, 494
NlaIV GGNNCC 1 cut(s) 160
NmeAIII GCCGAG 1 cut(s) 280
NmuCI GTSAC 1 cut(s) 995
NruI TCGCGA 1 cut(s) 263
NspI RCATGY 1 cut(s) 494
PaeI GCATGC 1 cut(s) 494
PaqCI CACCTGC 1 cut(s) 143
PctI GAATGC 1 cut(s) 120
PfeI GAWTC 3 cut(s) 82, 428, 778
PkrI GCNGC 5 cut(s) 60, 128, 140, 457, 799
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
Ppu21I YACGTR 1 cut(s) 317
Psp124BI GAGCTC 1 cut(s) 261
Psp6I CCWGG 3 cut(s) 62, 329, 485
PspGI CCWGG 3 cut(s) 62, 329, 485
PspN4I GGNNCC 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 797
RruI TCGCGA 1 cut(s) 263
RsaI GTAC 2 cut(s) 29, 241
RsaNI GTAC 2 cut(s) 28, 240
SacI GAGCTC 1 cut(s) 261
SaqAI TTAA 5 cut(s) 168, 621, 674, 813, 843
SatI GCNGC 5 cut(s) 59, 127, 139, 456, 798
Sau3AI GATC 1 cut(s) 66
ScaI AGTACT 1 cut(s) 29
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 3 cut(s) 64, 331, 487
SduI GDGCHC 2 cut(s) 251, 261
SfaNI GCATC 3 cut(s) 96, 385, 499
SfcI CTRYAG 1 cut(s) 837
SphI GCATGC 1 cut(s) 494
SsiI CCGC 6 cut(s) 35, 100, 138, 703, 857, 878
SspI AATATT 1 cut(s) 219
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 3 cut(s) 62, 329, 485
StyI CCWWGG 2 cut(s) 408, 459
TaaI ACNGT 4 cut(s) 499, 686, 826, 995
TaiI ACGT 4 cut(s) 13, 203, 319, 450
TaqI TCGA 4 cut(s) 45, 53, 781, 926
TatI WGTACW 1 cut(s) 27
TauI GCSGC 1 cut(s) 141
TfiI GAWTC 3 cut(s) 82, 428, 778
Tru1I TTAA 5 cut(s) 168, 621, 674, 813, 843
Tru9I TTAA 5 cut(s) 168, 621, 674, 813, 843
TscAI CASTG 2 cut(s) 829, 1000
TseFI GTSAC 1 cut(s) 995
TseI GCWGC 4 cut(s) 58, 126, 455, 797
Tsp45I GTSAC 1 cut(s) 995
TspDTI ATGAA 7 cut(s) 281, 354, 485, 494, 588, 651, 666
TspGWI ACGGA 1 cut(s) 529
TspRI CASTG 2 cut(s) 829, 1000
XapI RAATTY 6 cut(s) 164, 210, 283, 627, 803, 922
XceI RCATGY 1 cut(s) 494
ZraI GACGTC 1 cut(s) 448
ZrmI AGTACT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.