Rmu_sc0017546.1_g000001

Coiled-coil domain-containing protein 94

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0017546.1
OG0006725
Physical Location & Seq
Forward (+)
340 .. 558
219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0017546.1_g000001.1.cds

Sequence Viewer

Length: 219 bp
atgggagagcgtaaggttctcaacaagtactacccgccggacttcgacccgtccaagctgccccgagtccggcggcccaagaaccagcagattaaggtccgtatgatgcttccgatgagcatcagatgcaacacctgcggcaactacatctacagggcaccaaattcaactcccggaaagaagatgtcgtcggcgaggtacgtggatcaccaatcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

72

Amino Acids

8.45

Weight (kDa)

10.29

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 157
AciI CCGC 3 cut(s) 35, 73, 138
AclWI GGATC 1 cut(s) 213
AcsI RAATTY 1 cut(s) 163
AfaI GTAC 2 cut(s) 29, 200
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 1 cut(s) 168
AluBI AGCT 1 cut(s) 58
AluI AGCT 1 cut(s) 58
AlwI GGATC 1 cut(s) 213
Ama87I CYCGRG 1 cut(s) 63
AoxI GGCC 1 cut(s) 74
ApeKI GCWGC 1 cut(s) 58
ApoI RAATTY 1 cut(s) 163
AspS9I GGNCC 2 cut(s) 75, 97
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 200
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 1 cut(s) 97
BaeGI GKGCMC 1 cut(s) 160
BanI GGYRCC 1 cut(s) 157
BbvI GCAGC 1 cut(s) 45
BcnI CCSGG 1 cut(s) 174
BfmI CTRYAG 1 cut(s) 151
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 3 cut(s) 59, 74, 139
BlsI GCNGC 3 cut(s) 60, 75, 140
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 1 cut(s) 174
Bme18I GGWCC 1 cut(s) 97
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 2 cut(s) 75, 97
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 3 cut(s) 96, 116, 129
BpuMI CCSGG 1 cut(s) 174
BsaAI YACGTR 1 cut(s) 202
BsaBI GATNNNNATC 1 cut(s) 119
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse8I GATNNNNATC 1 cut(s) 119
BseJI GATNNNNATC 1 cut(s) 119
BseLI CCNNNNNNNGG 1 cut(s) 69
BseSI GKGCMC 1 cut(s) 160
BseXI GCAGC 1 cut(s) 45
BshFI GGCC 1 cut(s) 76
BshNI GGYRCC 1 cut(s) 157
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 3 cut(s) 38, 70, 174
BslI CCNNNNNNNGG 1 cut(s) 69
BsnI GGCC 1 cut(s) 76
BsoBI CYCGRG 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 160
Bsp143I GATC 1 cut(s) 205
BspACI CCGC 3 cut(s) 35, 73, 138
BspANI GGCC 1 cut(s) 76
BspLI GGNNCC 1 cut(s) 159
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 1 cut(s) 213
BspT107I GGYRCC 1 cut(s) 157
BssMI GATC 1 cut(s) 205
BstAPI GCANNNNNTGC 2 cut(s) 126, 135
BstBAI YACGTR 1 cut(s) 202
BstKTI GATC 1 cut(s) 208
BstMBI GATC 1 cut(s) 205
BstMWI GCNNNNNNNGC 2 cut(s) 126, 135
BstSCI CCNGG 1 cut(s) 172
BstSFI CTRYAG 1 cut(s) 151
BstSLI GKGCMC 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 45
BsuRI GGCC 1 cut(s) 76
BveI ACCTGC 1 cut(s) 143
Cfr13I GGNCC 2 cut(s) 75, 97
Csp6I GTAC 2 cut(s) 28, 199
CviJI RGCY 2 cut(s) 58, 76
CviKI_1 RGCY 2 cut(s) 58, 76
CviQI GTAC 2 cut(s) 28, 199
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
Eco47I GGWCC 1 cut(s) 97
Eco88I CYCGRG 1 cut(s) 63
FaiI YATR 1 cut(s) 104
FauI CCCGC 1 cut(s) 42
Fnu4HI GCNGC 3 cut(s) 59, 74, 139
Fsp4HI GCNGC 3 cut(s) 59, 74, 139
GluI GCNGC 3 cut(s) 59, 74, 139
HaeIII GGCC 1 cut(s) 76
HapII CCGG 3 cut(s) 38, 70, 174
HinfI GANTC 1 cut(s) 66
HpaII CCGG 3 cut(s) 38, 70, 174
HphI GGTGA 1 cut(s) 200
Hpy188I TCNGA 2 cut(s) 114, 125
Hpy188III TCNNGA 1 cut(s) 216
Hpy99I CGWCG 1 cut(s) 193
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 1 cut(s) 129
HpyF10VI GCNNNNNNNGC 2 cut(s) 126, 135
HpySE526I ACGT 1 cut(s) 201
Kzo9I GATC 1 cut(s) 205
LpnPI CCDG 6 cut(s) 51, 83, 98, 139, 148, 187
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 3 cut(s) 96, 116, 129
MaeII ACGT 1 cut(s) 201
MalI GATC 1 cut(s) 207
MboI GATC 1 cut(s) 205
MboII GAAGA 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 1 cut(s) 163
MlyI GAGTC 1 cut(s) 75
MnlI CCTC 1 cut(s) 189
MseI TTAA 1 cut(s) 93
MspI CCGG 3 cut(s) 38, 70, 174
MspR9I CCNGG 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 126, 135
NciI CCSGG 1 cut(s) 174
NdeII GATC 1 cut(s) 205
NlaIV GGNNCC 1 cut(s) 159
PaqCI CACCTGC 1 cut(s) 143
PfoI TCCNGGA 1 cut(s) 172
PkrI GCNGC 3 cut(s) 60, 75, 140
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
Ppu21I YACGTR 1 cut(s) 202
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 2 cut(s) 75, 97
RsaI GTAC 2 cut(s) 29, 200
RsaNI GTAC 2 cut(s) 28, 199
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 3 cut(s) 59, 74, 139
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 2 cut(s) 75, 97
ScaI AGTACT 1 cut(s) 29
SchI GAGTC 1 cut(s) 75
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 1 cut(s) 160
SetI ASST 6 cut(s) 18, 60, 99, 137, 200, 204
SfaNI GCATC 3 cut(s) 96, 116, 129
SfcI CTRYAG 1 cut(s) 151
SinI GGWCC 1 cut(s) 97
Sse9I AATT 1 cut(s) 163
SsiI CCGC 3 cut(s) 35, 73, 138
StyD4I CCNGG 1 cut(s) 172
TaiI ACGT 1 cut(s) 204
TaqI TCGA 1 cut(s) 45
TasI AATT 1 cut(s) 163
TatI WGTACW 1 cut(s) 27
TauI GCSGC 2 cut(s) 76, 141
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TseI GCWGC 1 cut(s) 58
TspGWI ACGGA 1 cut(s) 89
VpaK11BI GGWCC 1 cut(s) 97
XapI RAATTY 1 cut(s) 163
ZrmI AGTACT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.