Rh5DG194900

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006725
Physical Location & Seq
Forward (+)
21604911 .. 21608712
3802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG194900.1

Sequence Viewer

Length: 717 bp
ATGGGAGAGCGTAAGGTTCTCAACAAGTACTACCCGCCGGACTTCGACCCGTCCAAGCTGCCCCGAGTCCGGCGGCCCAAGAACCAGCAGATTAAGGTCCGTATGATGCTTCCGATGAGCATCAGATGCAACACCTGCGGCAACTACATCTACAAGGGCACCAAATTCAACTCCCGGAAAGAAGATGTCGTCGGCGAGACGTACTTGGGAATTCAAATCTTTAGATTTTACTTCAAGTGCACCAAGTGCTCAGCGGAGCTTGCGATGAAGACGGACCCGCAGAACTCAGACTATGTGGTGGAGGCCGGGGCTACACGAAATTTCGAACCTTGGCGTAATGAGGATGAGGAAGTGGATAAGGAGAAGCAGAAGAGGGAGGCCGAAGAAATGGGAGATGCCATGAAATCTTTGGAGAACAGAACCTTGGATTCGAAAAGAGAAATGGATATCCTTGCCGCATTGGATGAGATGAAGTCCATGAAGTCCAGGCATGCAACTGTGAGTGTGGATGAGATGTTGGAGGCTTTGCAACATACGGTTGCACACAAGGAGAAAAGGCTGGAAGAGGAGGATGAAGCAGTCATAAAATCGATCGTATTCCATAACTCAAAAGATTATGTTCGAAGGATTGATGATGATGATTTAGATGATGATGAAGATTTATTAGAGCCGTTAAGTGGCAATGGTGAAACGTCTAATCATCTGTCAAAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

238

Amino Acids

27.7

Weight (kDa)

5.35

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 9 - 220 1.8e-96 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 158
AciI CCGC 6 cut(s) 35, 73, 138, 254, 278, 456
AcsI RAATTY 3 cut(s) 164, 210, 319
AdeI CACNNNGTG 1 cut(s) 246
AfaI GTAC 2 cut(s) 29, 203
AfiI CCNNNNNNNGG 2 cut(s) 69, 677
AgsI TTSAA 3 cut(s) 169, 215, 235
AjnI CCWGG 1 cut(s) 485
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AluBI AGCT 2 cut(s) 58, 259
AluI AGCT 2 cut(s) 58, 259
Alw21I GWGCWC 2 cut(s) 242, 251
Alw26I GTCTC 1 cut(s) 191
Alw44I GTGCAC 1 cut(s) 238
Ama87I CYCGRG 1 cut(s) 63
AoxI GGCC 3 cut(s) 74, 303, 378
ApaLI GTGCAC 1 cut(s) 238
ApeKI GCWGC 1 cut(s) 58
ApoI RAATTY 3 cut(s) 164, 210, 319
AspS9I GGNCC 3 cut(s) 75, 97, 274
AsuC2I CCSGG 2 cut(s) 175, 307
AsuHPI GGTGA 1 cut(s) 698
AsuII TTCGAA 3 cut(s) 324, 431, 622
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 2 cut(s) 97, 274
BaeGI GKGCMC 2 cut(s) 161, 242
BanI GGYRCC 1 cut(s) 158
BbsI GAAGAC 1 cut(s) 275
Bbv12I GWGCWC 2 cut(s) 242, 251
BbvI GCAGC 1 cut(s) 45
BceAI ACGGC 1 cut(s) 655
BciT130I CCWGG 1 cut(s) 487
BcnI CCSGG 2 cut(s) 175, 307
BcoDI GTCTC 1 cut(s) 191
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 4 cut(s) 59, 74, 139, 456
BlpI GCTNAGC 1 cut(s) 250
BlsI GCNGC 4 cut(s) 60, 75, 140, 457
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 3 cut(s) 175, 307, 487
Bme18I GGWCC 2 cut(s) 97, 274
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 3 cut(s) 75, 97, 274
BmiI GGNNCC 2 cut(s) 160, 276
BmrFI CCNGG 3 cut(s) 175, 307, 487
BmsI GCATC 4 cut(s) 96, 116, 129, 385
BpiI GAAGAC 1 cut(s) 275
Bpu1102I GCTNAGC 1 cut(s) 250
Bpu14I TTCGAA 3 cut(s) 324, 431, 622
BpuMI CCSGG 2 cut(s) 175, 307
Bsa29I ATCGAT 1 cut(s) 590
BsaBI GATNNNNATC 1 cut(s) 119
BsaJI CCNNGG 3 cut(s) 306, 329, 423
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 677
Bse3DI GCAATG 1 cut(s) 688
Bse8I GATNNNNATC 1 cut(s) 119
BseBI CCWGG 1 cut(s) 487
BseCI ATCGAT 1 cut(s) 590
BseDI CCNNGG 3 cut(s) 306, 329, 423
BseGI GGATG 4 cut(s) 349, 469, 514, 577
BseJI GATNNNNATC 1 cut(s) 119
BseLI CCNNNNNNNGG 2 cut(s) 69, 677
BseMI GCAATG 1 cut(s) 688
BseMII CTCAG 2 cut(s) 264, 300
BseRI GAGGAG 1 cut(s) 581
BseSI GKGCMC 2 cut(s) 161, 242
BseXI GCAGC 1 cut(s) 45
Bsh1285I CGRYCG 1 cut(s) 594
BshFI GGCC 3 cut(s) 76, 305, 380
BshNI GGYRCC 1 cut(s) 158
BshVI ATCGAT 1 cut(s) 590
BsiEI CGRYCG 1 cut(s) 594
BsiHKAI GWGCWC 2 cut(s) 242, 251
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 4 cut(s) 38, 70, 175, 306
BslI CCNNNNNNNGG 2 cut(s) 69, 677
BsmAI GTCTC 1 cut(s) 191
BsmBI CGTCTC 1 cut(s) 191
BsnI GGCC 3 cut(s) 76, 305, 380
BsoBI CYCGRG 1 cut(s) 63
Bsp119I TTCGAA 3 cut(s) 324, 431, 622
Bsp1286I GDGCHC 3 cut(s) 161, 242, 251
Bsp143I GATC 1 cut(s) 591
Bsp1720I GCTNAGC 1 cut(s) 250
BspACI CCGC 6 cut(s) 35, 73, 138, 254, 278, 456
BspANI GGCC 3 cut(s) 76, 305, 380
BspCNI CTCAG 2 cut(s) 263, 299
BspDI ATCGAT 1 cut(s) 590
BspLI GGNNCC 2 cut(s) 160, 276
BspMI ACCTGC 1 cut(s) 143
BspT104I TTCGAA 3 cut(s) 324, 431, 622
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 688
BssECI CCNNGG 3 cut(s) 306, 329, 423
BssMI GATC 1 cut(s) 591
BssT1I CCWWGG 2 cut(s) 329, 423
Bst2UI CCWGG 1 cut(s) 487
Bst4CI ACNGT 2 cut(s) 499, 538
Bst6I CTCTTC 2 cut(s) 365, 558
BstAPI GCANNNNNTGC 3 cut(s) 126, 135, 246
BstBI TTCGAA 3 cut(s) 324, 431, 622
BstC8I GCNNGC 2 cut(s) 261, 492
BstDEI CTNAG 2 cut(s) 250, 286
BstF5I GGATG 4 cut(s) 349, 469, 514, 577
BstKTI GATC 1 cut(s) 594
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 1 cut(s) 591
BstMCI CGRYCG 1 cut(s) 594
BstMWI GCNNNNNNNGC 4 cut(s) 126, 135, 246, 260
BstNI CCWGG 1 cut(s) 487
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 3 cut(s) 173, 305, 485
BstSLI GKGCMC 2 cut(s) 161, 242
BstV1I GCAGC 1 cut(s) 45
BstV2I GAAGAC 1 cut(s) 275
Bsu15I ATCGAT 1 cut(s) 590
BsuRI GGCC 3 cut(s) 76, 305, 380
BsuTUI ATCGAT 1 cut(s) 590
BtgZI GCGATG 1 cut(s) 278
BtsCI GGATG 4 cut(s) 349, 469, 514, 577
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 261, 492
Cfr13I GGNCC 3 cut(s) 75, 97, 274
ClaI ATCGAT 1 cut(s) 590
Csp6I GTAC 2 cut(s) 28, 202
CviAII CATG 3 cut(s) 400, 478, 491
CviJI RGCY 9 cut(s) 58, 76, 259, 305, 311, 380, 524, 559, 670
CviKI_1 RGCY 9 cut(s) 58, 76, 259, 305, 311, 380, 524, 559, 670
CviQI GTAC 2 cut(s) 28, 202
DdeI CTNAG 2 cut(s) 250, 286
DpnI GATC 1 cut(s) 593
DpnII GATC 1 cut(s) 591
DraIII CACNNNGTG 1 cut(s) 246
Eam1104I CTCTTC 2 cut(s) 365, 558
EarI CTCTTC 2 cut(s) 365, 558
Eco130I CCWWGG 2 cut(s) 329, 423
Eco32I GATATC 1 cut(s) 448
Eco47I GGWCC 2 cut(s) 97, 274
Eco88I CYCGRG 1 cut(s) 63
EcoRI GAATTC 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 485
EcoRV GATATC 1 cut(s) 448
EcoT14I CCWWGG 2 cut(s) 329, 423
ErhI CCWWGG 2 cut(s) 329, 423
Esp3I CGTCTC 1 cut(s) 191
FaeI CATG 3 cut(s) 403, 481, 494
FatI CATG 3 cut(s) 399, 477, 490
FauI CCCGC 2 cut(s) 42, 285
Fnu4HI GCNGC 4 cut(s) 59, 74, 139, 456
FokI GGATG 4 cut(s) 356, 476, 521, 584
Fsp4HI GCNGC 4 cut(s) 59, 74, 139, 456
GluI GCNGC 4 cut(s) 59, 74, 139, 456
HaeIII GGCC 3 cut(s) 76, 305, 380
HapII CCGG 4 cut(s) 38, 70, 175, 306
Hin1II CATG 3 cut(s) 403, 481, 494
HinfI GANTC 2 cut(s) 66, 428
HpaII CCGG 4 cut(s) 38, 70, 175, 306
HphI GGTGA 1 cut(s) 698
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 3 cut(s) 114, 125, 289
Hpy8I GTNNAC 1 cut(s) 240
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 1 cut(s) 618
HpyCH4III ACNGT 2 cut(s) 499, 538
HpyCH4IV ACGT 2 cut(s) 200, 692
HpyCH4V TGCA 5 cut(s) 129, 240, 494, 529, 542
HpyF10VI GCNNNNNNNGC 4 cut(s) 126, 135, 246, 260
HpyF3I CTNAG 2 cut(s) 250, 286
HpySE526I ACGT 2 cut(s) 200, 692
Hsp92II CATG 3 cut(s) 403, 481, 494
Kzo9I GATC 1 cut(s) 591
LmnI GCTCC 1 cut(s) 256
LpnPI CCDG 9 cut(s) 51, 83, 98, 148, 188, 319, 472, 499, 545
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 4 cut(s) 96, 116, 129, 385
MaeII ACGT 2 cut(s) 200, 692
MalI GATC 1 cut(s) 593
MboI GATC 1 cut(s) 591
MboII GAAGA 6 cut(s) 194, 280, 382, 395, 575, 668
MhlI GDGCHC 3 cut(s) 161, 242, 251
MluCI AATT 3 cut(s) 164, 210, 319
MlyI GAGTC 1 cut(s) 75
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 8 cut(s) 295, 334, 340, 366, 370, 514, 559, 562
MseI TTAA 2 cut(s) 93, 674
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 4 cut(s) 38, 70, 175, 306
MspR9I CCNGG 3 cut(s) 175, 307, 487
MvaI CCWGG 1 cut(s) 487
MwoI GCNNNNNNNGC 4 cut(s) 126, 135, 246, 260
NciI CCSGG 2 cut(s) 175, 307
NdeII GATC 1 cut(s) 591
NlaIII CATG 3 cut(s) 403, 481, 494
NlaIV GGNNCC 2 cut(s) 160, 276
NspI RCATGY 1 cut(s) 494
NspV TTCGAA 3 cut(s) 324, 431, 622
PaeI GCATGC 1 cut(s) 494
PaqCI CACCTGC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 428
PfoI TCCNGGA 1 cut(s) 173
PkrI GCNGC 4 cut(s) 60, 75, 140, 457
Ple19I CGATCG 1 cut(s) 594
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
Psp6I CCWGG 1 cut(s) 485
PspGI CCWGG 1 cut(s) 485
PspN4I GGNNCC 2 cut(s) 160, 276
PspPI GGNCC 3 cut(s) 75, 97, 274
PvuI CGATCG 1 cut(s) 594
RsaI GTAC 2 cut(s) 29, 203
RsaNI GTAC 2 cut(s) 28, 202
SaqAI TTAA 2 cut(s) 93, 674
SatI GCNGC 4 cut(s) 59, 74, 139, 456
Sau3AI GATC 1 cut(s) 591
Sau96I GGNCC 3 cut(s) 75, 97, 274
ScaI AGTACT 1 cut(s) 29
SchI GAGTC 1 cut(s) 75
ScrFI CCNGG 3 cut(s) 175, 307, 487
SduI GDGCHC 3 cut(s) 161, 242, 251
SfaNI GCATC 4 cut(s) 96, 116, 129, 385
SfuI TTCGAA 3 cut(s) 324, 431, 622
SinI GGWCC 2 cut(s) 97, 274
SphI GCATGC 1 cut(s) 494
Sse9I AATT 3 cut(s) 164, 210, 319
SsiI CCGC 6 cut(s) 35, 73, 138, 254, 278, 456
StyD4I CCNGG 3 cut(s) 173, 305, 485
StyI CCWWGG 2 cut(s) 329, 423
TaaI ACNGT 2 cut(s) 499, 538
TaiI ACGT 2 cut(s) 203, 695
TaqI TCGA 5 cut(s) 45, 324, 431, 590, 622
TasI AATT 3 cut(s) 164, 210, 319
TatI WGTACW 1 cut(s) 27
TauI GCSGC 3 cut(s) 76, 141, 458
TfiI GAWTC 1 cut(s) 428
Tru1I TTAA 2 cut(s) 93, 674
Tru9I TTAA 2 cut(s) 93, 674
TseI GCWGC 1 cut(s) 58
TspDTI ATGAA 6 cut(s) 281, 416, 485, 494, 588, 669
TspGWI ACGGA 2 cut(s) 89, 287
VneI GTGCAC 1 cut(s) 238
VpaK11BI GGWCC 2 cut(s) 97, 274
XapI RAATTY 3 cut(s) 164, 210, 319
XceI RCATGY 1 cut(s) 494
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
ZrmI AGTACT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.