Rw5G017750

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006725
Physical Location & Seq
Forward (+)
22665394 .. 22670285
4892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G017750.1

Sequence Viewer

Length: 906 bp
ATGACGCTTCCGATGAGCATCAGATGCAACACCTGCGGCAACTACATCTACAAGGGCACCAAATTCAACTCCCGGAAAGAAGATGTCGTCGGCGAGTTGGGAATTCAAATCTTTAGATTTTACTTCAAGTGCACCAAGTGCTCGGCGGAGCTTGCGATGAAGACGGACCCGCAGAACTCAGACTATGTGGTGGAGGCCGGGGCTACACGAAATTTCGAACCTTGGCGAAATGAGGATGAGGAAGTGGATAAGGAGAAGCAGAAGAGGGAGGCCGAAGAAATGGGAGATGCCATGAAATCTTTGGAGAACAGAACCTTGGATTCGAAAAGAGAGATGGATATCCTTGCCGCTTTGGATGAGATGAAGTCCATGAAGTCCAGGCATGCAACTGTGAGTGTGGATGAGATGTTGGAGGCTTTACAACATACGGTTGCGCACAAGGCTGGAAGAGGGGATGAAGCAGTCATAAAATCGATCGTATTCCATAACTCAAAAGATTATGTTCGAAGGATTGATGATGATGATTTAGATGATGATGAAGATTTATTAGAGCCGTTAAGTGGCAATGGTGAAACGTCTAATCATCTGTCAAAGAAACAAAAGCTTACTCAAGATAATAGCAAACCAACAGATTTGTTGACAAAAACCAGTATTTCTGATAGCTCAAGCAGCAGAGATTCTAAAATCATTTTCACGTCTCCAACTGTTAGGCTTTCAGTAGTTAAGAAACCAGTGGTGGCTAAAGTTAACAATTTGGCAAAAGTGGACGAGAAAGAAGTTGGAGAGGAGTCTAAAAGTTTGACTAAAGATGAGAAAGAAGAGGACGGCGAGAATAAGACCGACGGTGGAAGCAACGTATTACAATCGCTGTTCCAAAACTACAACAGTGATGATAGTGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

301

Amino Acids

33.9

Weight (kDa)

4.93

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 1 - 298 6.6e-76 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 41
Acc16I TGCGCA 1 cut(s) 435
Acc36I ACCTGC 1 cut(s) 41
AccB1I GGYRCC 1 cut(s) 56
AciI CCGC 4 cut(s) 36, 146, 170, 348
AcsI RAATTY 3 cut(s) 62, 102, 211
AdeI CACNNNGTG 1 cut(s) 138
AfiI CCNNNNNNNGG 1 cut(s) 560
AgsI TTSAA 3 cut(s) 67, 107, 127
AjiI CACGTC 1 cut(s) 696
AjnI CCWGG 1 cut(s) 377
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AluBI AGCT 3 cut(s) 151, 604, 663
AluI AGCT 3 cut(s) 151, 604, 663
Alw21I GWGCWC 2 cut(s) 134, 143
Alw26I GTCTC 1 cut(s) 702
Alw44I GTGCAC 1 cut(s) 130
AoxI GGCC 2 cut(s) 195, 270
ApaLI GTGCAC 1 cut(s) 130
ApeKI GCWGC 1 cut(s) 669
ApoI RAATTY 3 cut(s) 62, 102, 211
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 1 cut(s) 166
AsuC2I CCSGG 2 cut(s) 73, 199
AsuHPI GGTGA 1 cut(s) 581
AsuII TTCGAA 3 cut(s) 216, 323, 505
AvaII GGWCC 1 cut(s) 166
BaeGI GKGCMC 2 cut(s) 59, 134
BanI GGYRCC 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 167
Bbv12I GWGCWC 2 cut(s) 134, 143
BbvI GCAGC 1 cut(s) 681
BccI CCATC 1 cut(s) 328
BceAI ACGGC 2 cut(s) 538, 841
BciT130I CCWGG 1 cut(s) 379
BcnI CCSGG 2 cut(s) 73, 199
BcoDI GTCTC 1 cut(s) 702
BfuAI ACCTGC 1 cut(s) 41
BisI GCNGC 3 cut(s) 37, 348, 670
BlsI GCNGC 3 cut(s) 38, 349, 671
Bme1390I CCNGG 3 cut(s) 73, 199, 379
Bme18I GGWCC 1 cut(s) 166
BmgBI CACGTC 1 cut(s) 696
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 2 cut(s) 58, 168
BmrFI CCNGG 3 cut(s) 73, 199, 379
BmsI GCATC 3 cut(s) 14, 27, 277
BpiI GAAGAC 1 cut(s) 167
Bpu14I TTCGAA 3 cut(s) 216, 323, 505
BpuEI CTTGAG 2 cut(s) 594, 649
BpuMI CCSGG 2 cut(s) 73, 199
Bsa29I ATCGAT 1 cut(s) 473
BsaBI GATNNNNATC 2 cut(s) 17, 338
BsaJI CCNNGG 3 cut(s) 198, 221, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 560
Bse1I ACTGG 2 cut(s) 648, 731
Bse3DI GCAATG 1 cut(s) 571
Bse8I GATNNNNATC 2 cut(s) 17, 338
BseBI CCWGG 1 cut(s) 379
BseCI ATCGAT 1 cut(s) 473
BseDI CCNNGG 3 cut(s) 198, 221, 315
BseGI GGATG 4 cut(s) 241, 361, 406, 460
BseJI GATNNNNATC 2 cut(s) 17, 338
BseLI CCNNNNNNNGG 1 cut(s) 560
BseMI GCAATG 1 cut(s) 571
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 2 cut(s) 648, 731
BseRI GAGGAG 1 cut(s) 800
BseSI GKGCMC 2 cut(s) 59, 134
BseXI GCAGC 1 cut(s) 681
Bsh1285I CGRYCG 1 cut(s) 477
BshFI GGCC 2 cut(s) 197, 272
BshNI GGYRCC 1 cut(s) 56
BshVI ATCGAT 1 cut(s) 473
BsiEI CGRYCG 1 cut(s) 477
BsiHKAI GWGCWC 2 cut(s) 134, 143
BsiSI CCGG 2 cut(s) 73, 198
BslI CCNNNNNNNGG 1 cut(s) 560
BsmAI GTCTC 1 cut(s) 702
BsmBI CGTCTC 1 cut(s) 702
BsnI GGCC 2 cut(s) 197, 272
Bsp119I TTCGAA 3 cut(s) 216, 323, 505
Bsp1286I GDGCHC 3 cut(s) 59, 134, 143
Bsp143I GATC 1 cut(s) 474
BspACI CCGC 4 cut(s) 36, 146, 170, 348
BspANI GGCC 2 cut(s) 197, 272
BspCNI CTCAG 1 cut(s) 191
BspDI ATCGAT 1 cut(s) 473
BspLI GGNNCC 2 cut(s) 58, 168
BspMI ACCTGC 1 cut(s) 41
BspT104I TTCGAA 3 cut(s) 216, 323, 505
BspT107I GGYRCC 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 571
BsrI ACTGG 2 cut(s) 648, 731
BssECI CCNNGG 3 cut(s) 198, 221, 315
BssMI GATC 1 cut(s) 474
BssT1I CCWWGG 2 cut(s) 221, 315
Bst2UI CCWGG 1 cut(s) 379
Bst4CI ACNGT 5 cut(s) 391, 430, 706, 845, 887
Bst6I CTCTTC 3 cut(s) 257, 442, 813
BstAPI GCANNNNNTGC 3 cut(s) 24, 33, 138
BstBI TTCGAA 3 cut(s) 216, 323, 505
BstC8I GCNNGC 2 cut(s) 153, 384
BstDEI CTNAG 1 cut(s) 178
BstF5I GGATG 4 cut(s) 241, 361, 406, 460
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 1 cut(s) 477
BstMAI GTCTC 1 cut(s) 702
BstMBI GATC 1 cut(s) 474
BstMCI CGRYCG 1 cut(s) 477
BstMWI GCNNNNNNNGC 6 cut(s) 24, 33, 138, 152, 440, 669
BstNI CCWGG 1 cut(s) 379
BstNSI RCATGY 1 cut(s) 386
BstSCI CCNGG 3 cut(s) 71, 197, 377
BstSLI GKGCMC 2 cut(s) 59, 134
BstV1I GCAGC 1 cut(s) 681
BstV2I GAAGAC 1 cut(s) 167
Bsu15I ATCGAT 1 cut(s) 473
BsuRI GGCC 2 cut(s) 197, 272
BsuTUI ATCGAT 1 cut(s) 473
BtgZI GCGATG 1 cut(s) 170
BtrI CACGTC 1 cut(s) 696
BtsCI GGATG 4 cut(s) 241, 361, 406, 460
BtsIMutI CAGTG 2 cut(s) 738, 892
BveI ACCTGC 1 cut(s) 41
Cac8I GCNNGC 2 cut(s) 153, 384
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 1 cut(s) 166
ClaI ATCGAT 1 cut(s) 473
CseI GACGC 1 cut(s) 13
CviAII CATG 3 cut(s) 292, 370, 383
DdeI CTNAG 1 cut(s) 178
DpnI GATC 1 cut(s) 476
DpnII GATC 1 cut(s) 474
DraIII CACNNNGTG 1 cut(s) 138
Eam1104I CTCTTC 3 cut(s) 257, 442, 813
EarI CTCTTC 3 cut(s) 257, 442, 813
EciI GGCGGA 1 cut(s) 161
Eco130I CCWWGG 2 cut(s) 221, 315
Eco32I GATATC 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 377
EcoRV GATATC 1 cut(s) 340
EcoT14I CCWWGG 2 cut(s) 221, 315
ErhI CCWWGG 2 cut(s) 221, 315
Esp3I CGTCTC 1 cut(s) 702
FaeI CATG 3 cut(s) 295, 373, 386
FaiI YATR 8 cut(s) 186, 293, 371, 384, 426, 467, 486, 501
FatI CATG 3 cut(s) 291, 369, 382
FauI CCCGC 1 cut(s) 177
Fnu4HI GCNGC 3 cut(s) 37, 348, 670
FokI GGATG 4 cut(s) 248, 368, 413, 467
Fsp4HI GCNGC 3 cut(s) 37, 348, 670
FspI TGCGCA 1 cut(s) 435
GlaI GCGC 1 cut(s) 435
GluI GCNGC 3 cut(s) 37, 348, 670
HaeIII GGCC 2 cut(s) 197, 272
HapII CCGG 2 cut(s) 73, 198
HgaI GACGC 1 cut(s) 13
HhaI GCGC 1 cut(s) 436
Hin1II CATG 3 cut(s) 295, 373, 386
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HincII GTYRAC 2 cut(s) 639, 748
HindII GTYRAC 2 cut(s) 639, 748
HindIII AAGCTT 1 cut(s) 602
HinfI GANTC 3 cut(s) 320, 677, 788
HpaI GTTAAC 1 cut(s) 748
HpaII CCGG 2 cut(s) 73, 198
HphI GGTGA 1 cut(s) 581
Hpy166II GTNNAC 4 cut(s) 132, 639, 748, 766
Hpy188I TCNGA 4 cut(s) 12, 23, 181, 658
Hpy188III TCNNGA 1 cut(s) 611
Hpy8I GTNNAC 4 cut(s) 132, 639, 748, 766
Hpy99I CGWCG 2 cut(s) 92, 845
HpyAV CCTTC 1 cut(s) 501
HpyCH4III ACNGT 5 cut(s) 391, 430, 706, 845, 887
HpyCH4IV ACGT 3 cut(s) 575, 695, 855
HpyCH4V TGCA 3 cut(s) 27, 132, 386
HpyF10VI GCNNNNNNNGC 6 cut(s) 24, 33, 138, 152, 440, 669
HpyF3I CTNAG 1 cut(s) 178
HpySE526I ACGT 3 cut(s) 575, 695, 855
Hsp92II CATG 3 cut(s) 295, 373, 386
HspAI GCGC 1 cut(s) 434
KspAI GTTAAC 1 cut(s) 748
Kzo9I GATC 1 cut(s) 474
LmnI GCTCC 1 cut(s) 148
LpnPI CCDG 8 cut(s) 46, 86, 211, 364, 391, 429, 661, 744
Lsp1109I GCAGC 1 cut(s) 681
LweI GCATC 3 cut(s) 14, 27, 277
MaeII ACGT 3 cut(s) 575, 695, 855
MalI GATC 1 cut(s) 476
MboI GATC 1 cut(s) 474
MboII GAAGA 7 cut(s) 92, 172, 274, 287, 459, 551, 830
MhlI GDGCHC 3 cut(s) 59, 134, 143
MluCI AATT 4 cut(s) 62, 102, 211, 751
MlyI GAGTC 1 cut(s) 797
MmeI TCCRAC 3 cut(s) 390, 725, 760
MnlI CCTC 9 cut(s) 187, 226, 232, 258, 262, 406, 443, 778, 814
MseI TTAA 3 cut(s) 557, 723, 747
MspI CCGG 2 cut(s) 73, 198
MspR9I CCNGG 3 cut(s) 73, 199, 379
MvaI CCWGG 1 cut(s) 379
MwoI GCNNNNNNNGC 6 cut(s) 24, 33, 138, 152, 440, 669
NciI CCSGG 2 cut(s) 73, 199
NdeII GATC 1 cut(s) 474
NlaIII CATG 3 cut(s) 295, 373, 386
NlaIV GGNNCC 2 cut(s) 58, 168
NmeAIII GCCGAG 1 cut(s) 122
NsbI TGCGCA 1 cut(s) 435
NspI RCATGY 1 cut(s) 386
NspV TTCGAA 3 cut(s) 216, 323, 505
PaeI GCATGC 1 cut(s) 386
PaqCI CACCTGC 1 cut(s) 41
PfeI GAWTC 2 cut(s) 320, 677
PfoI TCCNGGA 1 cut(s) 71
PkrI GCNGC 3 cut(s) 38, 349, 671
Ple19I CGATCG 1 cut(s) 477
PleI GAGTC 1 cut(s) 796
PpsI GAGTC 1 cut(s) 796
Psp6I CCWGG 1 cut(s) 377
PspGI CCWGG 1 cut(s) 377
PspN4I GGNNCC 2 cut(s) 58, 168
PspPI GGNCC 1 cut(s) 166
PvuI CGATCG 1 cut(s) 477
SaqAI TTAA 3 cut(s) 557, 723, 747
SatI GCNGC 3 cut(s) 37, 348, 670
Sau3AI GATC 1 cut(s) 474
Sau96I GGNCC 1 cut(s) 166
SchI GAGTC 1 cut(s) 797
ScrFI CCNGG 3 cut(s) 73, 199, 379
SduI GDGCHC 3 cut(s) 59, 134, 143
SetI ASST 9 cut(s) 35, 153, 223, 317, 578, 606, 665, 698, 858
SfaNI GCATC 3 cut(s) 14, 27, 277
SfuI TTCGAA 3 cut(s) 216, 323, 505
SinI GGWCC 1 cut(s) 166
SmlI CTYRAG 2 cut(s) 609, 664
SmoI CTYRAG 2 cut(s) 609, 664
SphI GCATGC 1 cut(s) 386
Sse9I AATT 4 cut(s) 62, 102, 211, 751
SsiI CCGC 4 cut(s) 36, 146, 170, 348
StyD4I CCNGG 3 cut(s) 71, 197, 377
StyI CCWWGG 2 cut(s) 221, 315
TaaI ACNGT 5 cut(s) 391, 430, 706, 845, 887
TaiI ACGT 3 cut(s) 578, 698, 858
TaqI TCGA 4 cut(s) 216, 323, 473, 505
TaqII GACCGA 1 cut(s) 854
TasI AATT 4 cut(s) 62, 102, 211, 751
TauI GCSGC 2 cut(s) 39, 350
TfiI GAWTC 2 cut(s) 320, 677
Tru1I TTAA 3 cut(s) 557, 723, 747
Tru9I TTAA 3 cut(s) 557, 723, 747
TscAI CASTG 2 cut(s) 738, 892
TseI GCWGC 1 cut(s) 669
TspDTI ATGAA 6 cut(s) 173, 308, 377, 386, 471, 552
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 2 cut(s) 738, 892
VneI GTGCAC 1 cut(s) 130
VpaK11BI GGWCC 1 cut(s) 166
XapI RAATTY 3 cut(s) 62, 102, 211
XceI RCATGY 1 cut(s) 386
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.