Rw5G017780

Coiled-coil domain-containing protein 94 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006725
Physical Location & Seq
Forward (+)
22697352 .. 22700767
3416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G017780.1

Sequence Viewer

Length: 957 bp
ATGGGAGAGCGTAAGGTTCTCAACAAGTACTACCCGCCGGACTTCGACCCGTCCAAGCTGCCCCGAGTCCGGCGGCCCAAGAACCAGCAGATTAAGGTCCGTATGATGCTTCCGATGAGCATCAGATGCAACACCTGCGGCAACTACATCTACAAGGGCACCAAATTCAACTCGCGGAAAGAAGATGTCGTCGGCGAGACGTACTTGGGAATTCAAATCTTTAGATTTTACTTCAAGTGCACCAAGTGCTCGGCGGAGCTTGCGATGAAGACGGACCCGCAGAACTCAGACTATGTGGTGGAGGCCGGGGCTACACGAAATTTCGAACCTTGGCGTAATGAGGATGAGGAAGTGGATAAGGAGAAGCAGAAGAGGGAGGCCGAAGAAATGGGAGATGCCATGAAATCTTTGGAGAACAGAACCTTGGATTCTAAAAGAGAGATGGATATCCTTGCCGCATTGGATGAGATGAAGTCCATGAAGTCCAGGCATGCAACTGTGAGTGTGGATGAGATGTTGGAGGCTTTACAACATACGGTTGCGCACAAGGAGAAAAGGCTGGAAGAGGAGGATGAAGCAGTCATAAAATCGATCGTATTCCATCCGTTAAGTGGCAATGGTGAAACGTCTAATCATCTGTCAAAGAAACAAAAGCTTACTCAAGATAATAGCAAACCAACAGATTTGTTGACAAAAACCAGTATTTCTGATAGCTCAAGCAGCAGAGATTCTAAAATCATTTTCACCTCTCCAACTGTTAGGCTTTCAGTAGTTAAGAAACCAGTGGTGGCTAAAGTTAACAATTTGGCAAAAGTGGACGAGAAAGAAGTTGGAGAGGAGTCTAAAAGTTTGACTAAAGATGAGAAAGAAGAGGATAGCGAGAATAAGACCGACGGTGGAAGCAACGTATTACAATCGCTGTTCCAAAACTACAACAGTGATGATAGTGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF572

Protein Analysis

318

Amino Acids

36.28

Weight (kDa)

5.93

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Saf4_Yju2 PF04502 9 - 315 2.1e-97 Saf4/Yju2 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
Acc16I TGCGCA 1 cut(s) 543
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 158
AccII CGCG 1 cut(s) 175
AciI CCGC 7 cut(s) 35, 73, 138, 175, 254, 278, 456
AcsI RAATTY 3 cut(s) 164, 210, 319
AdeI CACNNNGTG 1 cut(s) 246
AfaI GTAC 2 cut(s) 29, 203
AfiI CCNNNNNNNGG 2 cut(s) 69, 611
AgsI TTSAA 3 cut(s) 169, 215, 235
AjnI CCWGG 1 cut(s) 485
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AluBI AGCT 4 cut(s) 58, 259, 655, 714
AluI AGCT 4 cut(s) 58, 259, 655, 714
Alw21I GWGCWC 2 cut(s) 242, 251
Alw26I GTCTC 1 cut(s) 191
Alw44I GTGCAC 1 cut(s) 238
Ama87I CYCGRG 1 cut(s) 63
AoxI GGCC 3 cut(s) 74, 303, 378
ApaLI GTGCAC 1 cut(s) 238
ApeKI GCWGC 2 cut(s) 58, 720
ApoI RAATTY 3 cut(s) 164, 210, 319
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 3 cut(s) 75, 97, 274
AsuC2I CCSGG 1 cut(s) 307
AsuHPI GGTGA 2 cut(s) 632, 736
AsuII TTCGAA 1 cut(s) 324
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 2 cut(s) 97, 274
BaeGI GKGCMC 2 cut(s) 161, 242
BanI GGYRCC 1 cut(s) 158
BbsI GAAGAC 1 cut(s) 275
Bbv12I GWGCWC 2 cut(s) 242, 251
BbvI GCAGC 2 cut(s) 45, 732
BccI CCATC 2 cut(s) 436, 609
BciT130I CCWGG 1 cut(s) 487
BcnI CCSGG 1 cut(s) 307
BcoDI GTCTC 1 cut(s) 191
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 5 cut(s) 59, 74, 139, 456, 721
BlsI GCNGC 5 cut(s) 60, 75, 140, 457, 722
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 2 cut(s) 307, 487
Bme18I GGWCC 2 cut(s) 97, 274
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 3 cut(s) 75, 97, 274
BmiI GGNNCC 2 cut(s) 160, 276
BmrFI CCNGG 2 cut(s) 307, 487
BmsI GCATC 4 cut(s) 96, 116, 129, 385
BpiI GAAGAC 1 cut(s) 275
Bpu14I TTCGAA 1 cut(s) 324
BpuEI CTTGAG 2 cut(s) 645, 700
BpuMI CCSGG 1 cut(s) 307
Bsa29I ATCGAT 1 cut(s) 590
BsaBI GATNNNNATC 2 cut(s) 119, 446
BsaJI CCNNGG 3 cut(s) 306, 329, 423
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 611
Bse1I ACTGG 2 cut(s) 699, 782
Bse3DI GCAATG 1 cut(s) 622
Bse8I GATNNNNATC 2 cut(s) 119, 446
BseBI CCWGG 1 cut(s) 487
BseCI ATCGAT 1 cut(s) 590
BseDI CCNNGG 3 cut(s) 306, 329, 423
BseGI GGATG 5 cut(s) 349, 469, 514, 577, 601
BseJI GATNNNNATC 2 cut(s) 119, 446
BseLI CCNNNNNNNGG 2 cut(s) 69, 611
BseMI GCAATG 1 cut(s) 622
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 2 cut(s) 699, 782
BseRI GAGGAG 2 cut(s) 581, 851
BseSI GKGCMC 2 cut(s) 161, 242
BseXI GCAGC 2 cut(s) 45, 732
Bsh1236I CGCG 1 cut(s) 175
Bsh1285I CGRYCG 1 cut(s) 594
BshFI GGCC 3 cut(s) 76, 305, 380
BshNI GGYRCC 1 cut(s) 158
BshVI ATCGAT 1 cut(s) 590
BsiEI CGRYCG 1 cut(s) 594
BsiHKAI GWGCWC 2 cut(s) 242, 251
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 3 cut(s) 38, 70, 306
BslI CCNNNNNNNGG 2 cut(s) 69, 611
BsmAI GTCTC 1 cut(s) 191
BsmBI CGTCTC 1 cut(s) 191
BsnI GGCC 3 cut(s) 76, 305, 380
BsoBI CYCGRG 1 cut(s) 63
Bsp119I TTCGAA 1 cut(s) 324
Bsp1286I GDGCHC 3 cut(s) 161, 242, 251
Bsp143I GATC 1 cut(s) 591
BspACI CCGC 7 cut(s) 35, 73, 138, 175, 254, 278, 456
BspANI GGCC 3 cut(s) 76, 305, 380
BspCNI CTCAG 1 cut(s) 299
BspDI ATCGAT 1 cut(s) 590
BspFNI CGCG 1 cut(s) 175
BspLI GGNNCC 2 cut(s) 160, 276
BspMI ACCTGC 1 cut(s) 143
BspT104I TTCGAA 1 cut(s) 324
BspT107I GGYRCC 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 622
BsrI ACTGG 2 cut(s) 699, 782
BssECI CCNNGG 3 cut(s) 306, 329, 423
BssMI GATC 1 cut(s) 591
BssT1I CCWWGG 2 cut(s) 329, 423
Bst2UI CCWGG 1 cut(s) 487
Bst4CI ACNGT 5 cut(s) 499, 538, 757, 896, 938
Bst6I CTCTTC 3 cut(s) 365, 558, 864
BstAPI GCANNNNNTGC 3 cut(s) 126, 135, 246
BstBI TTCGAA 1 cut(s) 324
BstC8I GCNNGC 2 cut(s) 261, 492
BstDEI CTNAG 1 cut(s) 286
BstF5I GGATG 5 cut(s) 349, 469, 514, 577, 601
BstFNI CGCG 1 cut(s) 175
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 1 cut(s) 594
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 1 cut(s) 591
BstMCI CGRYCG 1 cut(s) 594
BstMWI GCNNNNNNNGC 5 cut(s) 126, 135, 246, 260, 720
BstNI CCWGG 1 cut(s) 487
BstNSI RCATGY 1 cut(s) 494
BstSCI CCNGG 2 cut(s) 305, 485
BstSLI GKGCMC 2 cut(s) 161, 242
BstUI CGCG 1 cut(s) 175
BstV1I GCAGC 2 cut(s) 45, 732
BstV2I GAAGAC 1 cut(s) 275
Bsu15I ATCGAT 1 cut(s) 590
BsuRI GGCC 3 cut(s) 76, 305, 380
BsuTUI ATCGAT 1 cut(s) 590
BtgZI GCGATG 1 cut(s) 278
BtsCI GGATG 5 cut(s) 349, 469, 514, 577, 601
BtsIMutI CAGTG 2 cut(s) 789, 943
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 261, 492
CfoI GCGC 1 cut(s) 544
Cfr13I GGNCC 3 cut(s) 75, 97, 274
ClaI ATCGAT 1 cut(s) 590
Csp6I GTAC 2 cut(s) 28, 202
CviAII CATG 3 cut(s) 400, 478, 491
CviQI GTAC 2 cut(s) 28, 202
DdeI CTNAG 1 cut(s) 286
DpnI GATC 1 cut(s) 593
DpnII GATC 1 cut(s) 591
DraIII CACNNNGTG 1 cut(s) 246
Eam1104I CTCTTC 3 cut(s) 365, 558, 864
EarI CTCTTC 3 cut(s) 365, 558, 864
EciI GGCGGA 1 cut(s) 269
Eco130I CCWWGG 2 cut(s) 329, 423
Eco32I GATATC 1 cut(s) 448
Eco47I GGWCC 2 cut(s) 97, 274
Eco88I CYCGRG 1 cut(s) 63
EcoRI GAATTC 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 485
EcoRV GATATC 1 cut(s) 448
EcoT14I CCWWGG 2 cut(s) 329, 423
ErhI CCWWGG 2 cut(s) 329, 423
Esp3I CGTCTC 1 cut(s) 191
FaeI CATG 3 cut(s) 403, 481, 494
FaiI YATR 7 cut(s) 104, 294, 401, 479, 492, 534, 584
FatI CATG 3 cut(s) 399, 477, 490
FauI CCCGC 2 cut(s) 42, 285
Fnu4HI GCNGC 5 cut(s) 59, 74, 139, 456, 721
FokI GGATG 5 cut(s) 356, 476, 521, 584, 588
Fsp4HI GCNGC 5 cut(s) 59, 74, 139, 456, 721
FspI TGCGCA 1 cut(s) 543
GlaI GCGC 1 cut(s) 543
GluI GCNGC 5 cut(s) 59, 74, 139, 456, 721
HaeIII GGCC 3 cut(s) 76, 305, 380
HapII CCGG 3 cut(s) 38, 70, 306
HhaI GCGC 1 cut(s) 544
Hin1II CATG 3 cut(s) 403, 481, 494
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 2 cut(s) 690, 799
HindII GTYRAC 2 cut(s) 690, 799
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 4 cut(s) 66, 428, 728, 839
HpaI GTTAAC 1 cut(s) 799
HpaII CCGG 3 cut(s) 38, 70, 306
HphI GGTGA 2 cut(s) 632, 736
Hpy166II GTNNAC 4 cut(s) 240, 690, 799, 817
Hpy188I TCNGA 4 cut(s) 114, 125, 289, 709
Hpy188III TCNNGA 1 cut(s) 662
Hpy8I GTNNAC 4 cut(s) 240, 690, 799, 817
Hpy99I CGWCG 2 cut(s) 194, 896
HpyCH4III ACNGT 5 cut(s) 499, 538, 757, 896, 938
HpyCH4IV ACGT 3 cut(s) 200, 626, 906
HpyCH4V TGCA 3 cut(s) 129, 240, 494
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 135, 246, 260, 720
HpyF3I CTNAG 1 cut(s) 286
HpySE526I ACGT 3 cut(s) 200, 626, 906
Hsp92II CATG 3 cut(s) 403, 481, 494
HspAI GCGC 1 cut(s) 542
KspAI GTTAAC 1 cut(s) 799
Kzo9I GATC 1 cut(s) 591
LmnI GCTCC 1 cut(s) 256
Lsp1109I GCAGC 2 cut(s) 45, 732
LweI GCATC 4 cut(s) 96, 116, 129, 385
MaeII ACGT 3 cut(s) 200, 626, 906
MalI GATC 1 cut(s) 593
MboI GATC 1 cut(s) 591
MboII GAAGA 6 cut(s) 194, 280, 382, 395, 575, 881
MhlI GDGCHC 3 cut(s) 161, 242, 251
MluCI AATT 4 cut(s) 164, 210, 319, 802
MlyI GAGTC 2 cut(s) 75, 848
MmeI TCCRAC 3 cut(s) 498, 776, 811
MseI TTAA 4 cut(s) 93, 608, 774, 798
MspI CCGG 3 cut(s) 38, 70, 306
MspR9I CCNGG 2 cut(s) 307, 487
MvaI CCWGG 1 cut(s) 487
MvnI CGCG 1 cut(s) 175
MwoI GCNNNNNNNGC 5 cut(s) 126, 135, 246, 260, 720
NciI CCSGG 1 cut(s) 307
NdeII GATC 1 cut(s) 591
NlaIII CATG 3 cut(s) 403, 481, 494
NlaIV GGNNCC 2 cut(s) 160, 276
NmeAIII GCCGAG 1 cut(s) 230
NsbI TGCGCA 1 cut(s) 543
NspI RCATGY 1 cut(s) 494
NspV TTCGAA 1 cut(s) 324
PaeI GCATGC 1 cut(s) 494
PaqCI CACCTGC 1 cut(s) 143
PfeI GAWTC 2 cut(s) 428, 728
PkrI GCNGC 5 cut(s) 60, 75, 140, 457, 722
Ple19I CGATCG 1 cut(s) 594
PleI GAGTC 2 cut(s) 74, 847
PpsI GAGTC 2 cut(s) 74, 847
Psp6I CCWGG 1 cut(s) 485
PspGI CCWGG 1 cut(s) 485
PspN4I GGNNCC 2 cut(s) 160, 276
PspPI GGNCC 3 cut(s) 75, 97, 274
PvuI CGATCG 1 cut(s) 594
RsaI GTAC 2 cut(s) 29, 203
RsaNI GTAC 2 cut(s) 28, 202
SaqAI TTAA 4 cut(s) 93, 608, 774, 798
SatI GCNGC 5 cut(s) 59, 74, 139, 456, 721
Sau3AI GATC 1 cut(s) 591
Sau96I GGNCC 3 cut(s) 75, 97, 274
ScaI AGTACT 1 cut(s) 29
SchI GAGTC 2 cut(s) 75, 848
ScrFI CCNGG 2 cut(s) 307, 487
SduI GDGCHC 3 cut(s) 161, 242, 251
SfaNI GCATC 4 cut(s) 96, 116, 129, 385
SfuI TTCGAA 1 cut(s) 324
SinI GGWCC 2 cut(s) 97, 274
SmlI CTYRAG 2 cut(s) 660, 715
SmoI CTYRAG 2 cut(s) 660, 715
SphI GCATGC 1 cut(s) 494
Sse9I AATT 4 cut(s) 164, 210, 319, 802
SsiI CCGC 7 cut(s) 35, 73, 138, 175, 254, 278, 456
StyD4I CCNGG 2 cut(s) 305, 485
StyI CCWWGG 2 cut(s) 329, 423
TaaI ACNGT 5 cut(s) 499, 538, 757, 896, 938
TaiI ACGT 3 cut(s) 203, 629, 909
TaqI TCGA 3 cut(s) 45, 324, 590
TaqII GACCGA 1 cut(s) 905
TasI AATT 4 cut(s) 164, 210, 319, 802
TatI WGTACW 1 cut(s) 27
TauI GCSGC 3 cut(s) 76, 141, 458
TfiI GAWTC 2 cut(s) 428, 728
Tru1I TTAA 4 cut(s) 93, 608, 774, 798
Tru9I TTAA 4 cut(s) 93, 608, 774, 798
TscAI CASTG 2 cut(s) 789, 943
TseI GCWGC 2 cut(s) 58, 720
TspDTI ATGAA 5 cut(s) 281, 416, 485, 494, 588
TspGWI ACGGA 3 cut(s) 89, 287, 594
TspRI CASTG 2 cut(s) 789, 943
VneI GTGCAC 1 cut(s) 238
VpaK11BI GGWCC 2 cut(s) 97, 274
XapI RAATTY 3 cut(s) 164, 210, 319
XceI RCATGY 1 cut(s) 494
XcmI CCANNNNNNNNNTGG 2 cut(s) 406, 608
ZrmI AGTACT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.