FvH4_3g20820

Phytohormone-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004227
Physical Location & Seq
Forward (+)
13903899 .. 13904811
913 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20820.t1

Sequence Viewer

Length: 468 bp
ATGGTTAAGGAAATCAAAACAGAAACCAAAGTTGGTGTTGGGATTGCAGCCTTATGGAAGGCCTTGTCAAAAGATTTGAGATTTCTGGCTCCGAAAATTGCACCTAATTTGATCAAGGATGTGGAACTCATAGAAGGAGATGGTACCAGCACCGGTACAGTCTTGCTCTTCAGCTTTGGCACCGATGCATCAAATATAGTTTACCAGGAGGAGAAGATTGTGGAGCTTGATGAGTCTTTGCATAGATTTGGCCTACAGGTGTTAAAAGGAGGCCACTTGAACCATGGGTTTTCTTCATACAAAACAAGTTTCCAACTTGCTGGAATTCAAGAGCAGGAGACCCTGGTGAGTATCACAGTCACTTATGAGTCTGCAGTTGAAGATACTACCATGCCATCAAAATCAACCCAAGCTTCTATAGCCTTCATCAAGAGCCTGGAATCCTATTTGTTAAATGCTGCCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

156

Amino Acids

16.84

Weight (kDa)

5.19

Isoelectric Point (pI)

29.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 2 - 152 1.1e-15 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 143
AccB1I GGYRCC 2 cut(s) 143, 179
AciI CCGC 1 cut(s) 462
AcsI RAATTY 1 cut(s) 324
AcuI CTGAAG 1 cut(s) 154
AfaI GTAC 2 cut(s) 145, 157
AgeI ACCGGT 1 cut(s) 152
AgsI TTSAA 3 cut(s) 280, 329, 380
AjnI CCWGG 3 cut(s) 204, 342, 435
AjuI GAANNNNNNNTTGG 2 cut(s) 15, 47
AluBI AGCT 3 cut(s) 174, 226, 413
AluI AGCT 3 cut(s) 174, 226, 413
Alw26I GTCTC 1 cut(s) 332
AoxI GGCC 3 cut(s) 60, 250, 271
ApeKI GCWGC 2 cut(s) 47, 458
ApoI RAATTY 1 cut(s) 324
AsiGI ACCGGT 1 cut(s) 152
Asp718I GGTACC 1 cut(s) 143
AsuHPI GGTGA 1 cut(s) 358
BanI GGYRCC 2 cut(s) 143, 179
BbvI GCAGC 2 cut(s) 59, 445
BccI CCATC 2 cut(s) 134, 403
BciT130I CCWGG 3 cut(s) 206, 344, 437
BclI TGATCA 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 332
BfaI CTAG 1 cut(s) 466
BfmI CTRYAG 3 cut(s) 254, 372, 417
BisI GCNGC 3 cut(s) 48, 459, 462
BlsI GCNGC 3 cut(s) 49, 460, 463
Bme1390I CCNGG 3 cut(s) 206, 344, 437
BmiI GGNNCC 3 cut(s) 90, 145, 181
BmrFI CCNGG 3 cut(s) 206, 344, 437
BmsI GCATC 2 cut(s) 175, 197
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 2 cut(s) 283, 342
BsaWI WCCGGW 1 cut(s) 152
Bse118I RCCGGY 1 cut(s) 152
BseBI CCWGG 3 cut(s) 206, 344, 437
BseDI CCNNGG 2 cut(s) 283, 342
BseGI GGATG 1 cut(s) 124
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 2 cut(s) 59, 445
BshFI GGCC 3 cut(s) 62, 252, 273
BshNI GGYRCC 2 cut(s) 143, 179
BshTI ACCGGT 1 cut(s) 152
BsiSI CCGG 1 cut(s) 153
BsmAI GTCTC 1 cut(s) 332
BsnI GGCC 3 cut(s) 62, 252, 273
Bso31I GGTCTC 1 cut(s) 332
Bsp143I GATC 1 cut(s) 111
Bsp19I CCATGG 1 cut(s) 283
BspACI CCGC 1 cut(s) 462
BspANI GGCC 3 cut(s) 62, 252, 273
BspLI GGNNCC 3 cut(s) 90, 145, 181
BspMAI CTGCAG 1 cut(s) 376
BspQI GCTCTTC 1 cut(s) 173
BspT107I GGYRCC 2 cut(s) 143, 179
BspTNI GGTCTC 1 cut(s) 332
BsrFI RCCGGY 1 cut(s) 152
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 2 cut(s) 283, 342
BssMI GATC 1 cut(s) 111
BssT1I CCWWGG 1 cut(s) 283
Bst2UI CCWGG 3 cut(s) 206, 344, 437
Bst4CI ACNGT 2 cut(s) 160, 358
Bst6I CTCTTC 1 cut(s) 173
BstDSI CCRYGG 1 cut(s) 283
BstF5I GGATG 1 cut(s) 124
BstKTI GATC 1 cut(s) 114
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 1 cut(s) 111
BstMWI GCNNNNNNNGC 1 cut(s) 419
BstNI CCWGG 3 cut(s) 206, 344, 437
BstSCI CCNGG 3 cut(s) 204, 342, 435
BstSFI CTRYAG 3 cut(s) 254, 372, 417
BstV1I GCAGC 2 cut(s) 59, 445
BstXI CCANNNNNNTGG 1 cut(s) 320
BsuRI GGCC 3 cut(s) 62, 252, 273
BtgI CCRYGG 1 cut(s) 283
BtsCI GGATG 1 cut(s) 124
Cfr10I RCCGGY 1 cut(s) 152
Csp6I GTAC 2 cut(s) 144, 156
CspAI ACCGGT 1 cut(s) 152
CviAII CATG 2 cut(s) 284, 391
CviQI GTAC 2 cut(s) 144, 156
DpnI GATC 1 cut(s) 113
DpnII GATC 1 cut(s) 111
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 283
Eco147I AGGCCT 1 cut(s) 62
Eco31I GGTCTC 1 cut(s) 332
Eco57I CTGAAG 1 cut(s) 154
EcoRI GAATTC 1 cut(s) 324
EcoRII CCWGG 3 cut(s) 204, 342, 435
EcoT14I CCWWGG 1 cut(s) 283
EcoT22I ATGCAT 1 cut(s) 190
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 2 cut(s) 287, 394
FaiI YATR 9 cut(s) 55, 131, 197, 243, 285, 298, 366, 392, 419
FatI CATG 2 cut(s) 283, 390
FbaI TGATCA 1 cut(s) 111
Fnu4HI GCNGC 3 cut(s) 48, 459, 462
FokI GGATG 1 cut(s) 131
Fsp4HI GCNGC 3 cut(s) 48, 459, 462
FspBI CTAG 1 cut(s) 466
GluI GCNGC 3 cut(s) 48, 459, 462
HaeIII GGCC 3 cut(s) 62, 252, 273
HapII CCGG 1 cut(s) 153
Hin1II CATG 2 cut(s) 287, 394
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 3 cut(s) 233, 368, 440
HpaII CCGG 1 cut(s) 153
HphI GGTGA 1 cut(s) 358
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 1 cut(s) 93
Hpy188III TCNNGA 2 cut(s) 329, 430
Hpy8I GTNNAC 1 cut(s) 202
HpyAV CCTTC 3 cut(s) 52, 128, 433
HpyCH4III ACNGT 2 cut(s) 160, 358
HpyCH4V TGCA 5 cut(s) 47, 101, 188, 241, 374
HpyF10VI GCNNNNNNNGC 1 cut(s) 419
Hsp92II CATG 2 cut(s) 287, 394
KpnI GGTACC 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 111
Kzo9I GATC 1 cut(s) 111
LguI GCTCTTC 1 cut(s) 173
LmnI GCTCC 2 cut(s) 94, 223
Lsp1109I GCAGC 2 cut(s) 59, 445
LweI GCATC 2 cut(s) 175, 197
MaeI CTAG 1 cut(s) 466
MaeIII GTNAC 1 cut(s) 358
MalI GATC 1 cut(s) 113
MboI GATC 1 cut(s) 111
MboII GAAGA 4 cut(s) 160, 226, 285, 392
MluCI AATT 3 cut(s) 96, 106, 324
MlyI GAGTC 2 cut(s) 242, 377
MmeI TCCRAC 1 cut(s) 337
MnlI CCTC 2 cut(s) 202, 263
Mph1103I ATGCAT 1 cut(s) 190
MseI TTAA 3 cut(s) 6, 263, 452
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 3 cut(s) 206, 344, 437
MvaI CCWGG 3 cut(s) 206, 344, 437
MwoI GCNNNNNNNGC 1 cut(s) 419
NcoI CCATGG 1 cut(s) 283
NdeII GATC 1 cut(s) 111
NlaIII CATG 2 cut(s) 287, 394
NlaIV GGNNCC 3 cut(s) 90, 145, 181
NmuCI GTSAC 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 190
PceI AGGCCT 1 cut(s) 62
PciSI GCTCTTC 1 cut(s) 173
PfeI GAWTC 1 cut(s) 440
PinAI ACCGGT 1 cut(s) 152
PkrI GCNGC 3 cut(s) 49, 460, 463
PleI GAGTC 2 cut(s) 241, 376
PpsI GAGTC 2 cut(s) 241, 376
Psp6I CCWGG 3 cut(s) 204, 342, 435
PspGI CCWGG 3 cut(s) 204, 342, 435
PspN4I GGNNCC 3 cut(s) 90, 145, 181
PstI CTGCAG 1 cut(s) 376
RsaI GTAC 2 cut(s) 145, 157
RsaNI GTAC 2 cut(s) 144, 156
SapI GCTCTTC 1 cut(s) 173
SaqAI TTAA 3 cut(s) 6, 263, 452
SatI GCNGC 3 cut(s) 48, 459, 462
Sau3AI GATC 1 cut(s) 111
SchI GAGTC 2 cut(s) 242, 377
ScrFI CCNGG 3 cut(s) 206, 344, 437
SetI ASST 5 cut(s) 106, 176, 228, 261, 415
SfaNI GCATC 2 cut(s) 175, 197
SfcI CTRYAG 3 cut(s) 254, 372, 417
Sse9I AATT 3 cut(s) 96, 106, 324
SseBI AGGCCT 1 cut(s) 62
SsiI CCGC 1 cut(s) 462
SspMI CTAG 1 cut(s) 466
StuI AGGCCT 1 cut(s) 62
StyD4I CCNGG 3 cut(s) 204, 342, 435
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 2 cut(s) 160, 358
TasI AATT 3 cut(s) 96, 106, 324
TauI GCSGC 1 cut(s) 464
TfiI GAWTC 1 cut(s) 440
Tru1I TTAA 3 cut(s) 6, 263, 452
Tru9I TTAA 3 cut(s) 6, 263, 452
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 2 cut(s) 47, 458
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 2 cut(s) 285, 415
XapI RAATTY 1 cut(s) 324
XcmI CCANNNNNNNNNTGG 1 cut(s) 281
XspI CTAG 1 cut(s) 466
Zsp2I ATGCAT 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.