MD03G1220400.v1.1

Phytohormone-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004227
Physical Location & Seq
Reverse (-)
30459694 .. 30461245
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1220400.v1.1.491

Sequence Viewer

Length: 456 bp
ATGGTTGTCAAAACGAAACAAGATCAGATAACAGTTGGCGTTGCAGTTGAAGCCCTGTGGAAAGCTATGGCTTTGGATGCAGTTACTGTCATACCAAAGATAATGTCTAGTGTCCGCAGTATCGATGTGATTGAAGGAGATGGTGGCCTTGGTTCCGTTCTGCGCTTCAACCTTGCCACTTGGTCCGATGGAGCACAGACAGAAAAGATTGTGGAACTTGATGAGTCTCATTATCGATATGCTTTACAAGTCATAAAAGGCCCGGCGCTAACACTGCGCAATTTTTCGGCATTGACAACATTTTTCCAACTGAAGAAAATCGGAGAGCAGGAGACATTGGTTGATATGAAAGTGGTATACGAGACTGAGAAAGAAGAAGTTAATAGCAGCGAGATTGCAATGCAGCCTGTAACTTCTTACGTTCAACTTCTCGAGAAATATTTGCTAGAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

152

Amino Acids

16.82

Weight (kDa)

4.84

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 150 1.7e-11 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 278
AccI GTMKAC 1 cut(s) 357
AciI CCGC 1 cut(s) 115
AcuI CTGAAG 1 cut(s) 332
AgsI TTSAA 4 cut(s) 50, 134, 169, 425
AluBI AGCT 1 cut(s) 65
AluI AGCT 1 cut(s) 65
Alw21I GWGCWC 1 cut(s) 196
Alw26I GTCTC 3 cut(s) 231, 326, 356
AlwNI CAGNNNCTG 1 cut(s) 86
Ama87I CYCGRG 1 cut(s) 431
AoxI GGCC 2 cut(s) 145, 259
ApeKI GCWGC 2 cut(s) 387, 403
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
AspLEI GCGC 3 cut(s) 165, 268, 279
AspS9I GGNCC 2 cut(s) 183, 260
AsuC2I CCSGG 1 cut(s) 263
AvaI CYCGRG 1 cut(s) 431
AvaII GGWCC 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 196
BbvI GCAGC 2 cut(s) 399, 415
BccI CCATC 2 cut(s) 134, 182
BcnI CCSGG 1 cut(s) 263
BcoDI GTCTC 3 cut(s) 231, 326, 356
BfaI CTAG 2 cut(s) 108, 446
BfoI RGCGCY 1 cut(s) 269
BisI GCNGC 2 cut(s) 388, 404
BlsI GCNGC 2 cut(s) 389, 405
Bme1390I CCNGG 1 cut(s) 263
Bme18I GGWCC 1 cut(s) 183
BmeT110I CYCGRG 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 183, 260
BmiI GGNNCC 1 cut(s) 154
BmrFI CCNGG 1 cut(s) 263
BmsI GCATC 1 cut(s) 67
BpuMI CCSGG 1 cut(s) 263
Bsa29I ATCGAT 2 cut(s) 123, 235
BsaJI CCNNGG 1 cut(s) 148
Bse3DI GCAATG 1 cut(s) 405
BseCI ATCGAT 2 cut(s) 123, 235
BseDI CCNNGG 1 cut(s) 148
BseGI GGATG 1 cut(s) 82
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 1 cut(s) 357
BseXI GCAGC 2 cut(s) 399, 415
BshFI GGCC 2 cut(s) 147, 261
BshVI ATCGAT 2 cut(s) 123, 235
BsiHKAI GWGCWC 1 cut(s) 196
BsiHKCI CYCGRG 1 cut(s) 431
BsiSI CCGG 1 cut(s) 263
BsmAI GTCTC 3 cut(s) 231, 326, 356
BsnI GGCC 2 cut(s) 147, 261
BsoBI CYCGRG 1 cut(s) 431
Bsp1286I GDGCHC 1 cut(s) 196
Bsp143I GATC 1 cut(s) 22
BspACI CCGC 1 cut(s) 115
BspANI GGCC 2 cut(s) 147, 261
BspCNI CTCAG 1 cut(s) 358
BspDI ATCGAT 2 cut(s) 123, 235
BspLI GGNNCC 1 cut(s) 154
BsrDI GCAATG 1 cut(s) 405
BssECI CCNNGG 1 cut(s) 148
BssMI GATC 1 cut(s) 22
BssNAI GTATAC 1 cut(s) 358
BssT1I CCWWGG 1 cut(s) 148
Bst1107I GTATAC 1 cut(s) 358
Bst4CI ACNGT 2 cut(s) 34, 88
BstDEI CTNAG 1 cut(s) 366
BstF5I GGATG 1 cut(s) 82
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 3 cut(s) 165, 268, 279
BstKTI GATC 1 cut(s) 25
BstMAI GTCTC 3 cut(s) 231, 326, 356
BstMBI GATC 1 cut(s) 22
BstMWI GCNNNNNNNGC 3 cut(s) 50, 77, 274
BstSCI CCNGG 1 cut(s) 261
BstV1I GCAGC 2 cut(s) 399, 415
BstZ17I GTATAC 1 cut(s) 358
Bsu15I ATCGAT 2 cut(s) 123, 235
BsuRI GGCC 2 cut(s) 147, 261
BsuTUI ATCGAT 2 cut(s) 123, 235
BtsCI GGATG 1 cut(s) 82
BtsI GCAGTG 1 cut(s) 272
BtsIMutI CAGTG 1 cut(s) 272
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 3 cut(s) 165, 268, 279
Cfr13I GGNCC 2 cut(s) 183, 260
ClaI ATCGAT 2 cut(s) 123, 235
CviJI RGCY 6 cut(s) 53, 65, 71, 147, 261, 406
CviKI_1 RGCY 6 cut(s) 53, 65, 71, 147, 261, 406
DdeI CTNAG 1 cut(s) 366
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 332
Eco88I CYCGRG 1 cut(s) 431
EcoT14I CCWWGG 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 148
FaiI YATR 6 cut(s) 68, 92, 240, 254, 347, 358
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 2 cut(s) 388, 404
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 2 cut(s) 388, 404
FspBI CTAG 2 cut(s) 108, 446
FspI TGCGCA 1 cut(s) 278
GlaI GCGC 3 cut(s) 164, 267, 278
GluI GCNGC 2 cut(s) 388, 404
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 2 cut(s) 147, 261
HapII CCGG 1 cut(s) 263
HhaI GCGC 3 cut(s) 165, 268, 279
Hin6I GCGC 3 cut(s) 163, 266, 277
HinP1I GCGC 3 cut(s) 163, 266, 277
HinfI GANTC 2 cut(s) 224, 449
HpaII CCGG 1 cut(s) 263
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 3 cut(s) 27, 187, 323
Hpy188III TCNNGA 2 cut(s) 431, 433
Hpy8I GTNNAC 1 cut(s) 358
HpyAV CCTTC 1 cut(s) 128
HpyCH4III ACNGT 2 cut(s) 34, 88
HpyCH4IV ACGT 1 cut(s) 420
HpyCH4V TGCA 4 cut(s) 44, 80, 398, 403
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 77, 274
HpyF3I CTNAG 1 cut(s) 366
HpySE526I ACGT 1 cut(s) 420
HspAI GCGC 3 cut(s) 163, 266, 277
Kzo9I GATC 1 cut(s) 22
LmnI GCTCC 1 cut(s) 191
LpnPI CCDG 4 cut(s) 68, 276, 314, 420
Lsp1109I GCAGC 2 cut(s) 399, 415
LweI GCATC 1 cut(s) 67
MaeI CTAG 2 cut(s) 108, 446
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 2 cut(s) 82, 409
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 2 cut(s) 325, 386
MhlI GDGCHC 1 cut(s) 196
MluCI AATT 1 cut(s) 280
MlyI GAGTC 1 cut(s) 233
MmeI TCCRAC 1 cut(s) 331
MseI TTAA 2 cut(s) 381, 454
MspI CCGG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 263
MwoI GCNNNNNNNGC 3 cut(s) 50, 77, 274
NciI CCSGG 1 cut(s) 263
NdeII GATC 1 cut(s) 22
NlaIV GGNNCC 1 cut(s) 154
NsbI TGCGCA 1 cut(s) 278
PaeR7I CTCGAG 1 cut(s) 431
PfeI GAWTC 1 cut(s) 449
PkrI GCNGC 2 cut(s) 389, 405
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
PspN4I GGNNCC 1 cut(s) 154
PspPI GGNCC 2 cut(s) 183, 260
PstNI CAGNNNCTG 1 cut(s) 86
SaqAI TTAA 2 cut(s) 381, 454
SatI GCNGC 2 cut(s) 388, 404
Sau3AI GATC 1 cut(s) 22
Sau96I GGNCC 2 cut(s) 183, 260
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 1 cut(s) 263
SduI GDGCHC 1 cut(s) 196
SetI ASST 3 cut(s) 67, 174, 423
SfaNI GCATC 1 cut(s) 67
Sfr274I CTCGAG 1 cut(s) 431
SinI GGWCC 1 cut(s) 183
SlaI CTCGAG 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
Sse9I AATT 1 cut(s) 280
SsiI CCGC 1 cut(s) 115
SspI AATATT 1 cut(s) 440
SspMI CTAG 2 cut(s) 108, 446
StyD4I CCNGG 1 cut(s) 261
StyI CCWWGG 1 cut(s) 148
TaaI ACNGT 2 cut(s) 34, 88
TaiI ACGT 1 cut(s) 423
TaqI TCGA 3 cut(s) 123, 235, 432
TasI AATT 1 cut(s) 280
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 2 cut(s) 381, 454
Tru9I TTAA 2 cut(s) 381, 454
TscAI CASTG 1 cut(s) 279
TseI GCWGC 2 cut(s) 387, 403
TspDTI ATGAA 1 cut(s) 362
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 1 cut(s) 279
VpaK11BI GGWCC 1 cut(s) 183
XhoI CTCGAG 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 357
XspI CTAG 2 cut(s) 108, 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.