MD03G1220500.v1.1

Phytohormone-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004227
Physical Location & Seq
Reverse (-)
30465091 .. 30466045
955 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1220500.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGTTGTCAAAACGAAACAAGATCAGATAACAGTTGGTGTTGCAGTTGAAGCCCTGTGGAAAGCTATGGCTTTGGATGCAGTTATTGTCATACCAAAGATAATGTCTAATATAGTCCGCAGTATTGATGTGATTGAAGGAGATGGTGGCCTTGGTTCCGTTCTGCTCTTCAACCTTGCCACTTATAGTGTAGAGTCCAATCGAGTACAGACGGAAAAGATTGTGGAACTTGATGAATCTCAGTATCGATATGCTTTACAAGTCGTGAAAGGCCCGGCGCTAACACTGCGCAATTTTTCGGCATTGACATCATTTTTCCAACTGAGGAAAATGGGAGAGCAGGAGACATTGGTTGATATGAAAGTGGAATACGAGACTGAAAAAGAAGAAGCTAATAGCGGCGAGATTGCAATGCAGCCTGTAACTTCTTACATTCAACTTCTGGAGAAATATTTGTTAGAATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

156

Amino Acids

17.34

Weight (kDa)

4.69

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 154 2.3e-11 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 290
AciI CCGC 2 cut(s) 118, 399
AfaI GTAC 1 cut(s) 207
AgsI TTSAA 4 cut(s) 50, 137, 172, 437
AluBI AGCT 2 cut(s) 65, 392
AluI AGCT 2 cut(s) 65, 392
Alw26I GTCTC 2 cut(s) 338, 368
AoxI GGCC 2 cut(s) 148, 271
ApeKI GCWGC 1 cut(s) 415
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
AspLEI GCGC 2 cut(s) 280, 291
AspS9I GGNCC 1 cut(s) 272
AsuC2I CCSGG 1 cut(s) 275
BbvI GCAGC 1 cut(s) 427
BccI CCATC 1 cut(s) 137
BcnI CCSGG 1 cut(s) 275
BcoDI GTCTC 2 cut(s) 338, 368
BfoI RGCGCY 1 cut(s) 281
BisI GCNGC 2 cut(s) 400, 416
BlsI GCNGC 2 cut(s) 401, 417
Bme1390I CCNGG 1 cut(s) 275
BmgT120I GGNCC 1 cut(s) 272
BmiI GGNNCC 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 275
BmsI GCATC 1 cut(s) 67
BpmI CTGGAG 1 cut(s) 464
BpuMI CCSGG 1 cut(s) 275
Bsa29I ATCGAT 1 cut(s) 247
BsaJI CCNNGG 1 cut(s) 151
Bse3DI GCAATG 1 cut(s) 417
BseCI ATCGAT 1 cut(s) 247
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 1 cut(s) 82
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 2 cut(s) 254, 314
BseXI GCAGC 1 cut(s) 427
BshFI GGCC 2 cut(s) 150, 273
BshVI ATCGAT 1 cut(s) 247
BsiSI CCGG 1 cut(s) 275
BsmAI GTCTC 2 cut(s) 338, 368
BsnI GGCC 2 cut(s) 150, 273
Bsp143I GATC 1 cut(s) 22
BspACI CCGC 2 cut(s) 118, 399
BspANI GGCC 2 cut(s) 150, 273
BspCNI CTCAG 2 cut(s) 253, 315
BspDI ATCGAT 1 cut(s) 247
BspLI GGNNCC 1 cut(s) 157
BspQI GCTCTTC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 417
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 1 cut(s) 22
BssT1I CCWWGG 1 cut(s) 151
Bst4CI ACNGT 1 cut(s) 34
Bst6I CTCTTC 1 cut(s) 173
BstDEI CTNAG 2 cut(s) 240, 323
BstF5I GGATG 1 cut(s) 82
BstH2I RGCGCY 1 cut(s) 281
BstHHI GCGC 2 cut(s) 280, 291
BstKTI GATC 1 cut(s) 25
BstMAI GTCTC 2 cut(s) 338, 368
BstMBI GATC 1 cut(s) 22
BstMWI GCNNNNNNNGC 3 cut(s) 50, 77, 286
BstSCI CCNGG 1 cut(s) 273
BstV1I GCAGC 1 cut(s) 427
Bsu15I ATCGAT 1 cut(s) 247
BsuRI GGCC 2 cut(s) 150, 273
BsuTUI ATCGAT 1 cut(s) 247
BtsCI GGATG 1 cut(s) 82
BtsI GCAGTG 1 cut(s) 284
BtsIMutI CAGTG 1 cut(s) 284
CfoI GCGC 2 cut(s) 280, 291
Cfr13I GGNCC 1 cut(s) 272
ClaI ATCGAT 1 cut(s) 247
Csp6I GTAC 1 cut(s) 206
CviJI RGCY 7 cut(s) 53, 65, 71, 150, 273, 392, 418
CviKI_1 RGCY 7 cut(s) 53, 65, 71, 150, 273, 392, 418
CviQI GTAC 1 cut(s) 206
DdeI CTNAG 2 cut(s) 240, 323
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
Eam1104I CTCTTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 151
EcoT14I CCWWGG 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 151
FaiI YATR 6 cut(s) 68, 92, 113, 186, 252, 359
Fnu4HI GCNGC 2 cut(s) 400, 416
FokI GGATG 1 cut(s) 89
Fsp4HI GCNGC 2 cut(s) 400, 416
FspI TGCGCA 1 cut(s) 290
GlaI GCGC 2 cut(s) 279, 290
GluI GCNGC 2 cut(s) 400, 416
GsuI CTGGAG 1 cut(s) 464
HaeII RGCGCY 1 cut(s) 281
HaeIII GGCC 2 cut(s) 150, 273
HapII CCGG 1 cut(s) 275
HhaI GCGC 2 cut(s) 280, 291
Hin6I GCGC 2 cut(s) 278, 289
HinP1I GCGC 2 cut(s) 278, 289
HinfI GANTC 3 cut(s) 194, 236, 461
HpaII CCGG 1 cut(s) 275
Hpy188I TCNGA 1 cut(s) 27
Hpy188III TCNNGA 2 cut(s) 265, 443
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4V TGCA 4 cut(s) 44, 80, 410, 415
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 77, 286
HpyF3I CTNAG 2 cut(s) 240, 323
HspAI GCGC 2 cut(s) 278, 289
Kzo9I GATC 1 cut(s) 22
LguI GCTCTTC 1 cut(s) 173
LpnPI CCDG 5 cut(s) 68, 288, 326, 428, 432
Lsp1109I GCAGC 1 cut(s) 427
LweI GCATC 1 cut(s) 67
MaeIII GTNAC 1 cut(s) 421
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 2 cut(s) 160, 398
MluCI AATT 1 cut(s) 292
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 1 cut(s) 318
MseI TTAA 1 cut(s) 466
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 1 cut(s) 275
MwoI GCNNNNNNNGC 3 cut(s) 50, 77, 286
NciI CCSGG 1 cut(s) 275
NdeII GATC 1 cut(s) 22
NlaIV GGNNCC 1 cut(s) 157
NsbI TGCGCA 1 cut(s) 290
PciSI GCTCTTC 1 cut(s) 173
PfeI GAWTC 2 cut(s) 236, 461
PkrI GCNGC 2 cut(s) 401, 417
PleI GAGTC 1 cut(s) 202
PpsI GAGTC 1 cut(s) 202
PspN4I GGNNCC 1 cut(s) 157
PspPI GGNCC 1 cut(s) 272
RsaI GTAC 1 cut(s) 207
RsaNI GTAC 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 173
SaqAI TTAA 1 cut(s) 466
SatI GCNGC 2 cut(s) 400, 416
Sau3AI GATC 1 cut(s) 22
Sau96I GGNCC 1 cut(s) 272
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 275
SetI ASST 3 cut(s) 67, 177, 394
SfaNI GCATC 1 cut(s) 67
Sse9I AATT 1 cut(s) 292
SsiI CCGC 2 cut(s) 118, 399
SspI AATATT 1 cut(s) 452
StyD4I CCNGG 1 cut(s) 273
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 34
TaqI TCGA 2 cut(s) 202, 247
TasI AATT 1 cut(s) 292
TatI WGTACW 1 cut(s) 205
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 2 cut(s) 236, 461
Tru1I TTAA 1 cut(s) 466
Tru9I TTAA 1 cut(s) 466
TscAI CASTG 1 cut(s) 291
TseI GCWGC 1 cut(s) 415
TspDTI ATGAA 2 cut(s) 249, 374
TspGWI ACGGA 2 cut(s) 148, 227
TspRI CASTG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.